Pedobacter sp. AR-3-17

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Pedobacter; unclassified Pedobacter

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3357 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U1BTU2|A0A4U1BTU2_9SPHI Uncharacterized protein OS=Pedobacter sp. AR-3-17 OX=2571271 GN=FA046_17095 PE=4 SV=1
MM1 pKa = 7.71RR2 pKa = 11.84KK3 pKa = 9.21IFSTFIMIICLGVSVQSFAQTCNTVPVINSFSPNTGFIGSKK44 pKa = 10.28VSIFGANFDD53 pKa = 4.87PNTISNNVVYY63 pKa = 10.36FGSTKK68 pKa = 9.88ATVVSATFGQLEE80 pKa = 4.53VIVPVGASTAPISVTNQCNRR100 pKa = 11.84TAYY103 pKa = 9.47STVSFNGIFCPTPLDD118 pKa = 3.43NQTYY122 pKa = 9.64NNRR125 pKa = 11.84SFDD128 pKa = 3.65LQGISGAYY136 pKa = 10.53NMISQDD142 pKa = 3.78LDD144 pKa = 3.99LDD146 pKa = 4.02GKK148 pKa = 10.2PEE150 pKa = 4.28VISSNGGSLTVARR163 pKa = 11.84NNSTPGALSFTALNTNGGGAGIYY186 pKa = 10.34AADD189 pKa = 4.04FDD191 pKa = 5.23GDD193 pKa = 3.9GKK195 pKa = 11.17KK196 pKa = 10.62DD197 pKa = 3.34ILATSQVSRR206 pKa = 11.84NTSTLGNISLATPQSIPGVSGYY228 pKa = 10.53QIAAGDD234 pKa = 3.85FNNDD238 pKa = 2.55GKK240 pKa = 10.7IDD242 pKa = 4.03IIGEE246 pKa = 3.93SGGFVRR252 pKa = 11.84VAFNTSTGPGNISFGPAQLFGNGSVTFRR280 pKa = 11.84CTGIQVADD288 pKa = 3.39VDD290 pKa = 4.55GDD292 pKa = 3.87GKK294 pKa = 9.47TDD296 pKa = 4.04FLASQGDD303 pKa = 3.86GNRR306 pKa = 11.84AVTIRR311 pKa = 11.84NTTTVGSFTPSAEE324 pKa = 3.99APEE327 pKa = 4.86FWASDD332 pKa = 3.42SNAADD337 pKa = 3.61GFGTYY342 pKa = 9.78PYY344 pKa = 9.77RR345 pKa = 11.84SQIADD350 pKa = 3.3FDD352 pKa = 4.48KK353 pKa = 11.06DD354 pKa = 3.5GKK356 pKa = 10.57IDD358 pKa = 3.85FTSCNYY364 pKa = 10.92SGDD367 pKa = 3.71TNVAIWRR374 pKa = 11.84NISTVGNITFAPTVNFPSPINNYY397 pKa = 9.87RR398 pKa = 11.84IGVGDD403 pKa = 3.71VDD405 pKa = 3.85GDD407 pKa = 4.11GYY409 pKa = 10.25PDD411 pKa = 3.72VVTKK415 pKa = 10.79SLGVNVFSVYY425 pKa = 10.95KK426 pKa = 9.19NTTSAVGAPTFATRR440 pKa = 11.84IDD442 pKa = 4.14YY443 pKa = 8.43TSSWQAEE450 pKa = 4.02VSGIVIGDD458 pKa = 3.81LDD460 pKa = 4.43GDD462 pKa = 4.44FVPDD466 pKa = 3.31IATSGTNSSRR476 pKa = 11.84ILFHH480 pKa = 7.4RR481 pKa = 11.84NTSFQADD488 pKa = 3.57NTAPTAIAQNVVAALAPNGTVTVTAAQVDD517 pKa = 4.1NGSSDD522 pKa = 3.22ACGIKK527 pKa = 9.78TITLSQTVFTCANIGANPVTLTVTDD552 pKa = 3.4NAGNVTTANAIVNVQPAAIIVSGQTTVCQGGTIPLIANLGDD593 pKa = 3.59TYY595 pKa = 11.17QWYY598 pKa = 10.15NNGVAISGATNQNYY612 pKa = 7.48TATQTGSYY620 pKa = 8.47TVAVTNSGGCSGTSLPTVVTVNDD643 pKa = 3.65NPVVEE648 pKa = 4.04ITGRR652 pKa = 11.84VDD654 pKa = 3.84AYY656 pKa = 11.19CPGEE660 pKa = 4.05ALIASQSSLYY670 pKa = 9.59QWSKK674 pKa = 10.83NGVIVANATLRR685 pKa = 11.84EE686 pKa = 4.53FIPNGPGTYY695 pKa = 9.7SVAVIDD701 pKa = 5.53LFGCSASGSVTISPDD716 pKa = 3.16TTIPTIALNGAAQLTVGSDD735 pKa = 3.38TQFNDD740 pKa = 3.54PSVTANDD747 pKa = 3.53NCSTNIVVTSNLNLSTPGVYY767 pKa = 9.72TISYY771 pKa = 9.08KK772 pKa = 10.88AVDD775 pKa = 3.72GSGNEE780 pKa = 4.13SVTITRR786 pKa = 11.84QVTVLDD792 pKa = 3.5ATAPIVNAGRR802 pKa = 11.84VTVQLDD808 pKa = 3.31ANGQVTVVPASLNNGSIDD826 pKa = 3.65NSNGALTFSLDD837 pKa = 3.63KK838 pKa = 11.29SVFDD842 pKa = 6.04CIDD845 pKa = 3.78LNSGTGGTLTAITSVGTVIRR865 pKa = 11.84EE866 pKa = 4.13NYY868 pKa = 9.84SVGAGFNPNTGEE880 pKa = 3.55YY881 pKa = 9.73WMPQWSGSTVYY892 pKa = 11.03KK893 pKa = 10.58LDD895 pKa = 3.23VAGNVLGSFNSGQNQMMQLWMDD917 pKa = 4.2ADD919 pKa = 4.11SEE921 pKa = 4.52TDD923 pKa = 3.6YY924 pKa = 11.0YY925 pKa = 8.77TANWTSNNPYY935 pKa = 10.1NFTRR939 pKa = 11.84INEE942 pKa = 4.18NGQKK946 pKa = 9.55VWTYY950 pKa = 11.06NVANGQYY957 pKa = 10.73ASAISTDD964 pKa = 3.04ANFAYY969 pKa = 10.73VIGYY973 pKa = 8.46GGNRR977 pKa = 11.84IDD979 pKa = 4.74VLNKK983 pKa = 7.99TTGQFQRR990 pKa = 11.84SISLPGQIYY999 pKa = 10.17LYY1001 pKa = 10.77GSLVVANDD1009 pKa = 4.19HH1010 pKa = 7.03IYY1012 pKa = 10.82FGGQTNGWGNVPNTWNAIHH1031 pKa = 6.53EE1032 pKa = 4.38FKK1034 pKa = 10.5IDD1036 pKa = 3.52GTYY1039 pKa = 10.38VGSISTPQAVYY1050 pKa = 10.13NASFDD1055 pKa = 4.32GEE1057 pKa = 4.72VIWASAFSNQNYY1069 pKa = 9.59GYY1071 pKa = 10.36KK1072 pKa = 10.03ISDD1075 pKa = 3.4GNAYY1079 pKa = 10.3SGNKK1083 pKa = 9.18GIEE1086 pKa = 4.29VVLTATDD1093 pKa = 3.43AAGNSATDD1101 pKa = 3.14VGTVVVEE1108 pKa = 4.62DD1109 pKa = 3.98NLAPTITLNGNQVVQVVVGATFTDD1133 pKa = 4.13PGANAIDD1140 pKa = 3.83NCSATVEE1147 pKa = 4.39TSGSVNVNLAGTYY1160 pKa = 8.91TILYY1164 pKa = 9.05KK1165 pKa = 10.84AIDD1168 pKa = 3.89GSGNEE1173 pKa = 4.01SAQISRR1179 pKa = 11.84TVQVIEE1185 pKa = 5.28LDD1187 pKa = 3.8VKK1189 pKa = 9.84TKK1191 pKa = 10.85NITVKK1196 pKa = 10.65LDD1198 pKa = 3.5ANGSYY1203 pKa = 9.27TLDD1206 pKa = 3.65PSEE1209 pKa = 4.35VDD1211 pKa = 2.97NGSILGGGSLSLNITQLDD1229 pKa = 4.0CSNIGNPVTVTVTATSSSGNTASGTAIVTVEE1260 pKa = 4.65DD1261 pKa = 3.44KK1262 pKa = 10.64FAPIVVTQNVTIQLNAEE1279 pKa = 4.26GFGSISPSSINNGSSDD1295 pKa = 3.38NCGIATIGLSKK1306 pKa = 9.65TAFDD1310 pKa = 4.68CSNIGTNTVTLTVIDD1325 pKa = 3.83VNGNVSTATAVVTVEE1340 pKa = 4.43DD1341 pKa = 3.83KK1342 pKa = 11.04VVPIVVTQNRR1352 pKa = 11.84TIQLDD1357 pKa = 3.5AAGNASITAAAINNGSSDD1375 pKa = 3.24NCAIASVVLDD1385 pKa = 3.67KK1386 pKa = 11.38TSFTCEE1392 pKa = 3.65NIGANTVTLTVTDD1405 pKa = 3.47VNGNVSSATAVVTVEE1420 pKa = 5.01DD1421 pKa = 4.55KK1422 pKa = 10.67IAPIVITQNRR1432 pKa = 11.84TIQLDD1437 pKa = 3.5AAGNASITAAAINNGSSDD1455 pKa = 3.32NCAIATVVLDD1465 pKa = 3.64QTSFTCEE1472 pKa = 3.41QVGANTVTLTVTDD1485 pKa = 3.47VNGNVSSATAVVTVEE1500 pKa = 5.01DD1501 pKa = 4.55KK1502 pKa = 10.67IAPIVITQNRR1512 pKa = 11.84TIQLDD1517 pKa = 3.5AAGNASITAAAINNGSSDD1535 pKa = 3.32NCAIATVVLDD1545 pKa = 3.64QTSFTCEE1552 pKa = 3.54QVGVNTVTLTVTDD1565 pKa = 3.47VNGNVSSATAVVTVEE1580 pKa = 5.01DD1581 pKa = 4.55KK1582 pKa = 10.67IAPIVITQNRR1592 pKa = 11.84TIQLDD1597 pKa = 3.5AAGNASITAAAINNGSSDD1615 pKa = 3.32NCAIATVVLDD1625 pKa = 3.64QTSFTCEE1632 pKa = 3.54QVGVNTVTLTVTDD1645 pKa = 3.53VNGNVSTATAVVTVEE1660 pKa = 5.17DD1661 pKa = 4.27NIKK1664 pKa = 10.49PIVITQNATIQLDD1677 pKa = 3.63AAGNASITAVAINNGSTDD1695 pKa = 2.93NCAIATIVLSKK1706 pKa = 9.74TAFDD1710 pKa = 4.37CSNVGANTVTLTVTDD1725 pKa = 3.53VNGNVSTATAVVTVVDD1741 pKa = 5.76AIAPIITQLAFQEE1754 pKa = 4.33ICFVASNTYY1763 pKa = 10.64SLPTLTATDD1772 pKa = 3.66NCSVASVNYY1781 pKa = 8.75VLSGATSRR1789 pKa = 11.84IGTGTDD1795 pKa = 2.97ASGTLNTGLTTISWTVTDD1813 pKa = 3.46VNGNVSTSSNDD1824 pKa = 2.75IRR1826 pKa = 11.84IWPLPVLSITPSNADD1841 pKa = 2.95AFF1843 pKa = 4.26

Molecular weight: 189.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U1C526|A0A4U1C526_9SPHI Translation initiation factor OS=Pedobacter sp. AR-3-17 OX=2571271 GN=FA046_04900 PE=3 SV=1
MM1 pKa = 7.57AHH3 pKa = 7.64PKK5 pKa = 10.37RR6 pKa = 11.84KK7 pKa = 8.88ISKK10 pKa = 8.89SRR12 pKa = 11.84RR13 pKa = 11.84DD14 pKa = 3.27KK15 pKa = 11.24RR16 pKa = 11.84RR17 pKa = 11.84THH19 pKa = 6.02YY20 pKa = 10.71KK21 pKa = 10.15AVAPSLWTCKK31 pKa = 8.07EE32 pKa = 3.85TGAVHH37 pKa = 6.83LPHH40 pKa = 7.23RR41 pKa = 11.84AYY43 pKa = 10.88NVDD46 pKa = 2.83GNLYY50 pKa = 10.45FRR52 pKa = 11.84GKK54 pKa = 10.67LIIEE58 pKa = 4.3NTSIAA63 pKa = 3.65

Molecular weight: 7.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3357

0

3357

1173386

32

2876

349.5

39.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.956 ± 0.053

0.757 ± 0.014

5.275 ± 0.03

5.936 ± 0.046

5.272 ± 0.039

6.547 ± 0.048

1.683 ± 0.025

7.99 ± 0.038

7.936 ± 0.046

9.527 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.104 ± 0.024

6.251 ± 0.05

3.488 ± 0.024

3.674 ± 0.022

3.261 ± 0.028

6.526 ± 0.039

5.665 ± 0.05

6.087 ± 0.033

1.086 ± 0.016

3.981 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski