Pseudomonas sp. LPB0260

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; unclassified Pseudomonas

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2912 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D5EEY3|A0A7D5EEY3_9PSED Electron transfer flavoprotein subunit beta/FixA family protein OS=Pseudomonas sp. LPB0260 OX=2614442 GN=LPB260_13180 PE=3 SV=1
MM1 pKa = 7.65EE2 pKa = 4.65EE3 pKa = 4.63VIDD6 pKa = 4.47QLRR9 pKa = 11.84EE10 pKa = 3.89LNEE13 pKa = 3.93PVPVPLEE20 pKa = 4.33LPDD23 pKa = 4.23EE24 pKa = 4.32EE25 pKa = 4.57TLVDD29 pKa = 3.51IQEE32 pKa = 4.35QILIHH37 pKa = 6.57LPYY40 pKa = 10.77ALRR43 pKa = 11.84EE44 pKa = 4.01FLLTVSDD51 pKa = 3.85VVYY54 pKa = 10.97GRR56 pKa = 11.84LEE58 pKa = 4.18PVTAADD64 pKa = 4.21PQSHH68 pKa = 6.45TYY70 pKa = 10.0LPEE73 pKa = 3.75VAATAWSLGVPRR85 pKa = 11.84EE86 pKa = 3.99YY87 pKa = 11.35VPLCEE92 pKa = 4.82DD93 pKa = 2.74QGNYY97 pKa = 9.09YY98 pKa = 10.28VVEE101 pKa = 4.02EE102 pKa = 5.09DD103 pKa = 3.63GTVLLWEE110 pKa = 5.1ADD112 pKa = 3.19TGEE115 pKa = 4.18INEE118 pKa = 4.32DD119 pKa = 3.76EE120 pKa = 4.38SWEE123 pKa = 4.53SVWHH127 pKa = 5.12WVRR130 pKa = 11.84DD131 pKa = 3.58VWLEE135 pKa = 3.97SS136 pKa = 3.39

Molecular weight: 15.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D5EET9|A0A7D5EET9_9PSED Molecular chaperone DnaJ OS=Pseudomonas sp. LPB0260 OX=2614442 GN=LPB260_12335 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.66RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2912

0

2912

970066

23

2448

333.1

36.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.667 ± 0.056

1.074 ± 0.017

5.163 ± 0.032

6.148 ± 0.042

3.564 ± 0.026

8.107 ± 0.044

2.27 ± 0.022

4.572 ± 0.035

3.159 ± 0.04

12.48 ± 0.091

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.178 ± 0.021

2.657 ± 0.027

4.841 ± 0.03

4.801 ± 0.043

6.869 ± 0.049

5.586 ± 0.033

4.156 ± 0.032

6.776 ± 0.033

1.427 ± 0.02

2.507 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski