Bromus-associated circular DNA virus 4

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4U841|A0A0B4U841_9VIRU Replication-associated protein OS=Bromus-associated circular DNA virus 4 OX=1590172 PE=3 SV=1
MM1 pKa = 7.71AGEE4 pKa = 4.12LSEE7 pKa = 4.67FCPHH11 pKa = 6.12SNPWRR16 pKa = 11.84HH17 pKa = 5.54EE18 pKa = 4.17PGEE21 pKa = 4.29GRR23 pKa = 11.84HH24 pKa = 5.31EE25 pKa = 4.04EE26 pKa = 3.95HH27 pKa = 7.24AKK29 pKa = 10.3VCGEE33 pKa = 3.87QDD35 pKa = 3.33SLRR38 pKa = 11.84PADD41 pKa = 4.36PEE43 pKa = 3.97HH44 pKa = 5.87VQPGRR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84DD52 pKa = 3.89LIRR55 pKa = 11.84CGYY58 pKa = 8.81EE59 pKa = 3.74EE60 pKa = 4.15QRR62 pKa = 11.84ATQPYY67 pKa = 8.37PRR69 pKa = 11.84NGNEE73 pKa = 3.87LARR76 pKa = 11.84SRR78 pKa = 11.84SVEE81 pKa = 3.7GLGIRR86 pKa = 11.84TSDD89 pKa = 3.47RR90 pKa = 11.84ADD92 pKa = 3.0SGGPLLGDD100 pKa = 4.75PIRR103 pKa = 11.84CHH105 pKa = 6.59WYY107 pKa = 9.57HH108 pKa = 6.45AVHH111 pKa = 6.48VDD113 pKa = 3.54GVLQTVPHH121 pKa = 6.44CKK123 pKa = 9.82GHH125 pKa = 6.01EE126 pKa = 4.04EE127 pKa = 5.16NLVFWCGPSPPHH139 pKa = 6.73YY140 pKa = 10.59SEE142 pKa = 4.03ATADD146 pKa = 3.59VRR148 pKa = 11.84HH149 pKa = 6.32SGEE152 pKa = 4.17AVCGRR157 pKa = 11.84DD158 pKa = 3.52SAGPGHH164 pKa = 6.19NRR166 pKa = 11.84PDD168 pKa = 3.35RR169 pKa = 11.84GFLAWALSPRR179 pKa = 11.84HH180 pKa = 5.53EE181 pKa = 4.79WIHH184 pKa = 6.28DD185 pKa = 3.82LSSVGLYY192 pKa = 7.81WQQQ195 pKa = 2.8

Molecular weight: 21.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4U9L1|A0A0B4U9L1_9VIRU Putative capsid protein OS=Bromus-associated circular DNA virus 4 OX=1590172 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84PSHH5 pKa = 5.97GRR7 pKa = 11.84AIPQRR12 pKa = 11.84LHH14 pKa = 6.58AATKK18 pKa = 9.93FAYY21 pKa = 9.6HH22 pKa = 6.88HH23 pKa = 6.55ARR25 pKa = 11.84GAAVKK30 pKa = 10.61AMAKK34 pKa = 9.06YY35 pKa = 9.95ARR37 pKa = 11.84SRR39 pKa = 11.84ATRR42 pKa = 11.84KK43 pKa = 10.01HH44 pKa = 5.42GGGFKK49 pKa = 10.25KK50 pKa = 10.23RR51 pKa = 11.84YY52 pKa = 6.39TPKK55 pKa = 9.93TVLGKK60 pKa = 10.6RR61 pKa = 11.84KK62 pKa = 9.03IASSAGGVSKK72 pKa = 10.54RR73 pKa = 11.84RR74 pKa = 11.84KK75 pKa = 8.31VYY77 pKa = 9.06SSKK80 pKa = 10.67RR81 pKa = 11.84IKK83 pKa = 10.23PYY85 pKa = 10.12SKK87 pKa = 10.19RR88 pKa = 11.84GGKK91 pKa = 9.25RR92 pKa = 11.84RR93 pKa = 11.84GGSFSNAIAKK103 pKa = 9.72VDD105 pKa = 3.53SSAFSLGPGPRR116 pKa = 11.84LFSGFRR122 pKa = 11.84RR123 pKa = 11.84TQQPTTVNCTISSRR137 pKa = 11.84VSSTPSQQAVQLLPTYY153 pKa = 9.86FFAGDD158 pKa = 4.13GSAGDD163 pKa = 4.38PSRR166 pKa = 11.84QSAFTIDD173 pKa = 3.91SYY175 pKa = 11.56ILNRR179 pKa = 11.84AEE181 pKa = 3.37RR182 pKa = 11.84WLANFQNSVPTQTPGGTNQGRR203 pKa = 11.84DD204 pKa = 3.4GMRR207 pKa = 11.84NTQKK211 pKa = 10.48FVVNKK216 pKa = 9.84IHH218 pKa = 7.04YY219 pKa = 7.39DD220 pKa = 3.4QQIRR224 pKa = 11.84NMSNQDD230 pKa = 2.77VDD232 pKa = 4.23VILYY236 pKa = 10.21DD237 pKa = 3.33VVMRR241 pKa = 11.84NNVQPNLTRR250 pKa = 11.84ATGTNLPVADD260 pKa = 4.75PLKK263 pKa = 10.44DD264 pKa = 3.38WEE266 pKa = 4.57SGLLIEE272 pKa = 5.08QIQGGLYY279 pKa = 10.31LGTRR283 pKa = 11.84LGAIGTTPFMSTAFCKK299 pKa = 10.34LYY301 pKa = 10.7RR302 pKa = 11.84IAKK305 pKa = 5.97VTKK308 pKa = 8.33KK309 pKa = 8.41TLSSGAVHH317 pKa = 6.42HH318 pKa = 6.08HH319 pKa = 7.05HH320 pKa = 6.32ITVKK324 pKa = 9.17PRR326 pKa = 11.84QMFDD330 pKa = 3.39TQVKK334 pKa = 9.83RR335 pKa = 11.84YY336 pKa = 9.66ADD338 pKa = 3.66EE339 pKa = 4.16TAPDD343 pKa = 4.48LDD345 pKa = 3.96TTGLIGVSSRR355 pKa = 11.84GPYY358 pKa = 9.77LPGMSGFTILVASGSIGNNNVEE380 pKa = 4.39KK381 pKa = 10.77AQIGYY386 pKa = 8.14TSTALDD392 pKa = 3.82VVTKK396 pKa = 10.78GSFSFSNFTRR406 pKa = 11.84EE407 pKa = 4.33RR408 pKa = 11.84RR409 pKa = 11.84FHH411 pKa = 6.97LNFDD415 pKa = 3.99GLATNVNQIVTDD427 pKa = 3.8DD428 pKa = 3.72TDD430 pKa = 5.08LIGPVQEE437 pKa = 4.28AA438 pKa = 3.48

Molecular weight: 47.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

990

195

438

330.0

36.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.475 ± 0.279

2.02 ± 0.791

5.859 ± 0.765

4.444 ± 1.522

4.141 ± 0.682

7.879 ± 1.437

3.838 ± 1.025

4.646 ± 0.721

5.152 ± 1.068

6.97 ± 0.504

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.313 ± 0.277

4.444 ± 0.489

5.556 ± 0.818

4.04 ± 0.511

7.677 ± 0.701

6.97 ± 0.998

6.465 ± 1.262

6.162 ± 0.234

1.818 ± 0.687

3.131 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski