Amino acid dipepetide frequency for Pseudomonas sp. MF4836

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.818AlaAla: 12.818 ± 0.117	1.213AlaCys: 1.213 ± 0.028	5.785AlaAsp: 5.785 ± 0.058	6.607AlaGlu: 6.607 ± 0.065	3.763AlaPhe: 3.763 ± 0.043	8.986AlaGly: 8.986 ± 0.083	2.202AlaHis: 2.202 ± 0.038	5.123AlaIle: 5.123 ± 0.055	3.628AlaLys: 3.628 ± 0.06	14.126AlaLeu: 14.126 ± 0.117	2.815AlaMet: 2.815 ± 0.034	2.949AlaAsn: 2.949 ± 0.046	4.936AlaPro: 4.936 ± 0.062	5.641AlaGln: 5.641 ± 0.055	6.937AlaArg: 6.937 ± 0.072	6.5AlaSer: 6.5 ± 0.068	5.003AlaThr: 5.003 ± 0.095	7.362AlaVal: 7.362 ± 0.068	1.649AlaTrp: 1.649 ± 0.031	2.644AlaTyr: 2.644 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	1.12CysAla: 1.12 ± 0.025	0.139CysCys: 0.139 ± 0.008	0.542CysAsp: 0.542 ± 0.015	0.523CysGlu: 0.523 ± 0.018	0.334CysPhe: 0.334 ± 0.012	0.952CysGly: 0.952 ± 0.023	0.257CysHis: 0.257 ± 0.014	0.444CysIle: 0.444 ± 0.014	0.276CysLys: 0.276 ± 0.013	1.145CysLeu: 1.145 ± 0.025	0.22CysMet: 0.22 ± 0.011	0.259CysAsn: 0.259 ± 0.01	0.465CysPro: 0.465 ± 0.015	0.404CysGln: 0.404 ± 0.014	0.586CysArg: 0.586 ± 0.017	0.611CysSer: 0.611 ± 0.017	0.462CysThr: 0.462 ± 0.014	0.684CysVal: 0.684 ± 0.016	0.16CysTrp: 0.16 ± 0.009	0.26CysTyr: 0.26 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	5.497AspAla: 5.497 ± 0.069	0.542AspCys: 0.542 ± 0.016	2.723AspAsp: 2.723 ± 0.042	3.186AspGlu: 3.186 ± 0.041	2.141AspPhe: 2.141 ± 0.037	4.4AspGly: 4.4 ± 0.059	1.104AspHis: 1.104 ± 0.023	2.568AspIle: 2.568 ± 0.034	1.851AspLys: 1.851 ± 0.028	5.9AspLeu: 5.9 ± 0.065	1.143AspMet: 1.143 ± 0.025	1.661AspAsn: 1.661 ± 0.033	2.907AspPro: 2.907 ± 0.04	2.322AspGln: 2.322 ± 0.036	2.935AspArg: 2.935 ± 0.039	3.082AspSer: 3.082 ± 0.048	2.351AspThr: 2.351 ± 0.037	3.34AspVal: 3.34 ± 0.04	0.961AspTrp: 0.961 ± 0.023	1.764AspTyr: 1.764 ± 0.032	0.0AspXaa: 0.0 ± 0.0
Glu	6.079GluAla: 6.079 ± 0.061	0.427GluCys: 0.427 ± 0.013	2.355GluAsp: 2.355 ± 0.033	2.84GluGlu: 2.84 ± 0.053	1.804GluPhe: 1.804 ± 0.028	3.714GluGly: 3.714 ± 0.044	1.738GluHis: 1.738 ± 0.033	2.656GluIle: 2.656 ± 0.038	1.946GluLys: 1.946 ± 0.04	6.786GluLeu: 6.786 ± 0.066	1.219GluMet: 1.219 ± 0.023	1.477GluAsn: 1.477 ± 0.027	2.509GluPro: 2.509 ± 0.037	3.989GluGln: 3.989 ± 0.057	4.547GluArg: 4.547 ± 0.06	2.664GluSer: 2.664 ± 0.038	2.351GluThr: 2.351 ± 0.032	4.0GluVal: 4.0 ± 0.046	0.702GluTrp: 0.702 ± 0.019	1.217GluTyr: 1.217 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	4.029PheAla: 4.029 ± 0.043	0.451PheCys: 0.451 ± 0.015	2.472PheAsp: 2.472 ± 0.035	2.092PheGlu: 2.092 ± 0.032	1.532PhePhe: 1.532 ± 0.034	3.237PheGly: 3.237 ± 0.046	0.72PheHis: 0.72 ± 0.017	1.93PheIle: 1.93 ± 0.031	1.35PheLys: 1.35 ± 0.03	3.16PheLeu: 3.16 ± 0.048	0.835PheMet: 0.835 ± 0.02	1.482PheAsn: 1.482 ± 0.029	1.418PhePro: 1.418 ± 0.028	1.244PheGln: 1.244 ± 0.026	1.681PheArg: 1.681 ± 0.032	2.776PheSer: 2.776 ± 0.038	1.901PheThr: 1.901 ± 0.051	2.534PheVal: 2.534 ± 0.036	0.584PheTrp: 0.584 ± 0.019	1.11PheTyr: 1.11 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	7.683GlyAla: 7.683 ± 0.071	0.947GlyCys: 0.947 ± 0.02	3.783GlyAsp: 3.783 ± 0.051	4.463GlyGlu: 4.463 ± 0.042	3.396GlyPhe: 3.396 ± 0.047	5.918GlyGly: 5.918 ± 0.086	1.842GlyHis: 1.842 ± 0.03	4.062GlyIle: 4.062 ± 0.048	3.195GlyLys: 3.195 ± 0.044	9.74GlyLeu: 9.74 ± 0.082	2.078GlyMet: 2.078 ± 0.035	2.44GlyAsn: 2.44 ± 0.042	2.773GlyPro: 2.773 ± 0.036	3.96GlyGln: 3.96 ± 0.047	4.795GlyArg: 4.795 ± 0.059	4.941GlySer: 4.941 ± 0.069	3.849GlyThr: 3.849 ± 0.069	5.995GlyVal: 5.995 ± 0.062	1.396GlyTrp: 1.396 ± 0.031	2.543GlyTyr: 2.543 ± 0.04	0.0GlyXaa: 0.0 ± 0.0
His	2.198HisAla: 2.198 ± 0.033	0.317HisCys: 0.317 ± 0.014	1.22HisAsp: 1.22 ± 0.025	1.179HisGlu: 1.179 ± 0.024	0.969HisPhe: 0.969 ± 0.023	2.05HisGly: 2.05 ± 0.032	0.626HisHis: 0.626 ± 0.022	0.989HisIle: 0.989 ± 0.022	0.656HisLys: 0.656 ± 0.018	2.674HisLeu: 2.674 ± 0.04	0.506HisMet: 0.506 ± 0.015	0.67HisAsn: 0.67 ± 0.017	1.434HisPro: 1.434 ± 0.029	1.052HisGln: 1.052 ± 0.025	1.268HisArg: 1.268 ± 0.024	1.443HisSer: 1.443 ± 0.028	0.94HisThr: 0.94 ± 0.021	1.263HisVal: 1.263 ± 0.024	0.475HisTrp: 0.475 ± 0.017	0.774HisTyr: 0.774 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	5.825IleAla: 5.825 ± 0.064	0.538IleCys: 0.538 ± 0.015	3.277IleAsp: 3.277 ± 0.043	3.312IleGlu: 3.312 ± 0.042	1.483IlePhe: 1.483 ± 0.027	4.585IleGly: 4.585 ± 0.052	0.962IleHis: 0.962 ± 0.021	2.041IleIle: 2.041 ± 0.036	1.772IleLys: 1.772 ± 0.031	4.042IleLeu: 4.042 ± 0.05	0.773IleMet: 0.773 ± 0.02	1.782IleAsn: 1.782 ± 0.031	2.179IlePro: 2.179 ± 0.038	1.618IleGln: 1.618 ± 0.028	2.737IleArg: 2.737 ± 0.038	3.213IleSer: 3.213 ± 0.041	2.51IleThr: 2.51 ± 0.04	3.088IleVal: 3.088 ± 0.04	0.535IleTrp: 0.535 ± 0.018	1.157IleTyr: 1.157 ± 0.02	0.0IleXaa: 0.0 ± 0.0
Lys	3.924LysAla: 3.924 ± 0.053	0.176LysCys: 0.176 ± 0.009	1.754LysAsp: 1.754 ± 0.031	1.579LysGlu: 1.579 ± 0.034	0.909LysPhe: 0.909 ± 0.024	2.514LysGly: 2.514 ± 0.038	0.77LysHis: 0.77 ± 0.018	1.621LysIle: 1.621 ± 0.03	1.374LysLys: 1.374 ± 0.036	3.689LysLeu: 3.689 ± 0.05	0.688LysMet: 0.688 ± 0.021	1.087LysAsn: 1.087 ± 0.025	2.035LysPro: 2.035 ± 0.042	1.533LysGln: 1.533 ± 0.027	2.242LysArg: 2.242 ± 0.036	1.903LysSer: 1.903 ± 0.032	1.864LysThr: 1.864 ± 0.032	2.746LysVal: 2.746 ± 0.037	0.346LysTrp: 0.346 ± 0.012	0.714LysTyr: 0.714 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	14.454LeuAla: 14.454 ± 0.108	1.236LeuCys: 1.236 ± 0.023	6.69LeuAsp: 6.69 ± 0.069	6.495LeuGlu: 6.495 ± 0.068	4.198LeuPhe: 4.198 ± 0.056	9.636LeuGly: 9.636 ± 0.093	2.56LeuHis: 2.56 ± 0.037	5.693LeuIle: 5.693 ± 0.065	4.391LeuLys: 4.391 ± 0.052	14.223LeuLeu: 14.223 ± 0.143	2.578LeuMet: 2.578 ± 0.042	3.79LeuAsn: 3.79 ± 0.045	6.448LeuPro: 6.448 ± 0.07	5.129LeuGln: 5.129 ± 0.055	7.3LeuArg: 7.3 ± 0.068	7.721LeuSer: 7.721 ± 0.066	5.765LeuThr: 5.765 ± 0.054	7.8LeuVal: 7.8 ± 0.072	1.431LeuTrp: 1.431 ± 0.027	2.68LeuTyr: 2.68 ± 0.036	0.0LeuXaa: 0.0 ± 0.0
Met	2.54MetAla: 2.54 ± 0.037	0.173MetCys: 0.173 ± 0.009	0.99MetAsp: 0.99 ± 0.022	0.88MetGlu: 0.88 ± 0.019	0.72MetPhe: 0.72 ± 0.023	1.635MetGly: 1.635 ± 0.032	0.491MetHis: 0.491 ± 0.015	1.112MetIle: 1.112 ± 0.021	0.847MetLys: 0.847 ± 0.022	2.676MetLeu: 2.676 ± 0.041	0.469MetMet: 0.469 ± 0.017	0.867MetAsn: 0.867 ± 0.022	1.302MetPro: 1.302 ± 0.027	1.035MetGln: 1.035 ± 0.021	1.399MetArg: 1.399 ± 0.025	1.733MetSer: 1.733 ± 0.031	1.413MetThr: 1.413 ± 0.023	1.475MetVal: 1.475 ± 0.028	0.165MetTrp: 0.165 ± 0.009	0.363MetTyr: 0.363 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.194AsnAla: 3.194 ± 0.041	0.295AsnCys: 0.295 ± 0.012	1.595AsnAsp: 1.595 ± 0.032	1.407AsnGlu: 1.407 ± 0.03	1.105AsnPhe: 1.105 ± 0.034	2.651AsnGly: 2.651 ± 0.053	0.606AsnHis: 0.606 ± 0.016	1.455AsnIle: 1.455 ± 0.029	0.943AsnLys: 0.943 ± 0.02	3.614AsnLeu: 3.614 ± 0.042	0.553AsnMet: 0.553 ± 0.017	1.039AsnAsn: 1.039 ± 0.032	2.006AsnPro: 2.006 ± 0.032	1.395AsnGln: 1.395 ± 0.029	1.885AsnArg: 1.885 ± 0.032	1.795AsnSer: 1.795 ± 0.037	1.524AsnThr: 1.524 ± 0.029	1.91AsnVal: 1.91 ± 0.038	0.482AsnTrp: 0.482 ± 0.016	0.883AsnTyr: 0.883 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	5.724ProAla: 5.724 ± 0.064	0.363ProCys: 0.363 ± 0.011	2.716ProAsp: 2.716 ± 0.038	3.168ProGlu: 3.168 ± 0.044	1.762ProPhe: 1.762 ± 0.028	4.039ProGly: 4.039 ± 0.045	1.103ProHis: 1.103 ± 0.022	2.008ProIle: 2.008 ± 0.03	1.537ProLys: 1.537 ± 0.027	6.042ProLeu: 6.042 ± 0.069	1.149ProMet: 1.149 ± 0.025	1.332ProAsn: 1.332 ± 0.027	1.982ProPro: 1.982 ± 0.035	2.659ProGln: 2.659 ± 0.041	2.655ProArg: 2.655 ± 0.035	2.745ProSer: 2.745 ± 0.036	2.273ProThr: 2.273 ± 0.034	3.663ProVal: 3.663 ± 0.054	0.786ProTrp: 0.786 ± 0.019	1.22ProTyr: 1.22 ± 0.022	0.0ProXaa: 0.0 ± 0.0
Gln	6.445GlnAla: 6.445 ± 0.071	0.344GlnCys: 0.344 ± 0.013	1.97GlnAsp: 1.97 ± 0.032	1.973GlnGlu: 1.973 ± 0.034	1.408GlnPhe: 1.408 ± 0.027	3.76GlnGly: 3.76 ± 0.051	1.216GlnHis: 1.216 ± 0.023	2.224GlnIle: 2.224 ± 0.036	1.267GlnLys: 1.267 ± 0.025	6.044GlnLeu: 6.044 ± 0.064	1.095GlnMet: 1.095 ± 0.021	1.166GlnAsn: 1.166 ± 0.026	2.62GlnPro: 2.62 ± 0.043	3.09GlnGln: 3.09 ± 0.053	3.853GlnArg: 3.853 ± 0.051	2.434GlnSer: 2.434 ± 0.034	2.073GlnThr: 2.073 ± 0.033	3.985GlnVal: 3.985 ± 0.056	0.794GlnTrp: 0.794 ± 0.021	1.033GlnTyr: 1.033 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	5.801ArgAla: 5.801 ± 0.054	0.596ArgCys: 0.596 ± 0.018	3.342ArgAsp: 3.342 ± 0.038	3.969ArgGlu: 3.969 ± 0.053	2.67ArgPhe: 2.67 ± 0.036	4.0ArgGly: 4.0 ± 0.042	1.69ArgHis: 1.69 ± 0.031	3.193ArgIle: 3.193 ± 0.04	2.009ArgLys: 2.009 ± 0.031	8.295ArgLeu: 8.295 ± 0.086	1.534ArgMet: 1.534 ± 0.029	1.914ArgAsn: 1.914 ± 0.029	2.721ArgPro: 2.721 ± 0.045	3.559ArgGln: 3.559 ± 0.047	4.062ArgArg: 4.062 ± 0.054	3.582ArgSer: 3.582 ± 0.038	2.643ArgThr: 2.643 ± 0.037	4.289ArgVal: 4.289 ± 0.049	1.052ArgTrp: 1.052 ± 0.024	2.037ArgTyr: 2.037 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	6.693SerAla: 6.693 ± 0.067	0.474SerCys: 0.474 ± 0.016	2.972SerAsp: 2.972 ± 0.039	3.057SerGlu: 3.057 ± 0.038	2.358SerPhe: 2.358 ± 0.034	5.533SerGly: 5.533 ± 0.065	1.37SerHis: 1.37 ± 0.025	2.967SerIle: 2.967 ± 0.038	1.824SerLys: 1.824 ± 0.033	7.486SerLeu: 7.486 ± 0.065	1.384SerMet: 1.384 ± 0.03	1.861SerAsn: 1.861 ± 0.039	2.818SerPro: 2.818 ± 0.034	2.698SerGln: 2.698 ± 0.033	3.759SerArg: 3.759 ± 0.046	3.79SerSer: 3.79 ± 0.058	3.093SerThr: 3.093 ± 0.052	4.145SerVal: 4.145 ± 0.051	0.848SerTrp: 0.848 ± 0.019	1.615SerTyr: 1.615 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	5.132ThrAla: 5.132 ± 0.063	0.442ThrCys: 0.442 ± 0.015	2.336ThrAsp: 2.336 ± 0.055	2.317ThrGlu: 2.317 ± 0.036	1.809ThrPhe: 1.809 ± 0.035	3.968ThrGly: 3.968 ± 0.058	1.037ThrHis: 1.037 ± 0.022	1.871ThrIle: 1.871 ± 0.031	1.082ThrLys: 1.082 ± 0.027	6.816ThrLeu: 6.816 ± 0.062	0.74ThrMet: 0.74 ± 0.021	1.165ThrAsn: 1.165 ± 0.028	3.162ThrPro: 3.162 ± 0.045	2.201ThrGln: 2.201 ± 0.036	3.058ThrArg: 3.058 ± 0.044	2.807ThrSer: 2.807 ± 0.056	2.356ThrThr: 2.356 ± 0.04	3.427ThrVal: 3.427 ± 0.059	0.754ThrTrp: 0.754 ± 0.02	1.305ThrTyr: 1.305 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	7.728ValAla: 7.728 ± 0.063	0.699ValCys: 0.699 ± 0.021	3.812ValAsp: 3.812 ± 0.048	4.245ValGlu: 4.245 ± 0.044	2.566ValPhe: 2.566 ± 0.033	5.19ValGly: 5.19 ± 0.058	1.428ValHis: 1.428 ± 0.028	3.779ValIle: 3.779 ± 0.047	2.264ValLys: 2.264 ± 0.04	8.329ValLeu: 8.329 ± 0.083	1.643ValMet: 1.643 ± 0.032	2.118ValAsn: 2.118 ± 0.04	3.208ValPro: 3.208 ± 0.041	2.906ValGln: 2.906 ± 0.04	4.134ValArg: 4.134 ± 0.04	4.389ValSer: 4.389 ± 0.053	3.506ValThr: 3.506 ± 0.049	5.149ValVal: 5.149 ± 0.063	0.842ValTrp: 0.842 ± 0.018	1.553ValTyr: 1.553 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	1.234TrpAla: 1.234 ± 0.023	0.164TrpCys: 0.164 ± 0.01	0.644TrpAsp: 0.644 ± 0.017	0.56TrpGlu: 0.56 ± 0.016	0.545TrpPhe: 0.545 ± 0.019	0.927TrpGly: 0.927 ± 0.021	0.416TrpHis: 0.416 ± 0.013	0.665TrpIle: 0.665 ± 0.018	0.459TrpLys: 0.459 ± 0.015	2.298TrpLeu: 2.298 ± 0.044	0.375TrpMet: 0.375 ± 0.014	0.485TrpAsn: 0.485 ± 0.015	0.686TrpPro: 0.686 ± 0.017	0.995TrpGln: 0.995 ± 0.025	1.087TrpArg: 1.087 ± 0.024	0.903TrpSer: 0.903 ± 0.023	0.639TrpThr: 0.639 ± 0.018	0.997TrpVal: 0.997 ± 0.022	0.255TrpTrp: 0.255 ± 0.01	0.33TrpTyr: 0.33 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.564TyrAla: 2.564 ± 0.036	0.307TyrCys: 0.307 ± 0.013	1.398TyrAsp: 1.398 ± 0.033	1.17TyrGlu: 1.17 ± 0.02	1.065TyrPhe: 1.065 ± 0.026	2.103TyrGly: 2.103 ± 0.033	0.568TyrHis: 0.568 ± 0.015	1.026TyrIle: 1.026 ± 0.026	0.8TyrLys: 0.8 ± 0.021	3.135TyrLeu: 3.135 ± 0.043	0.489TyrMet: 0.489 ± 0.016	0.796TyrAsn: 0.796 ± 0.021	1.317TyrPro: 1.317 ± 0.024	1.366TyrGln: 1.366 ± 0.026	2.035TyrArg: 2.035 ± 0.035	1.711TyrSer: 1.711 ± 0.037	1.267TyrThr: 1.267 ± 0.039	1.638TyrVal: 1.638 ± 0.028	0.447TyrTrp: 0.447 ± 0.014	0.736TyrTyr: 0.736 ± 0.02	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6768 proteins (2209861 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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