Bhendi yellow vein Haryana virus [2003:Karnal]

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Bhendi yellow vein Haryana virus

Average proteome isoelectric point is 8.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2DJ45|D2DJ45_9GEMI AC4 OS=Bhendi yellow vein Haryana virus [2003:Karnal] OX=658650 GN=AC4 PE=3 SV=1
MM1 pKa = 7.68APPSRR6 pKa = 11.84FRR8 pKa = 11.84INAKK12 pKa = 10.3NYY14 pKa = 8.19FLTYY18 pKa = 9.06PKK20 pKa = 10.53CSLTKK25 pKa = 10.69EE26 pKa = 4.09EE27 pKa = 5.47ALSQLQTLEE36 pKa = 4.24TPTSKK41 pKa = 10.73KK42 pKa = 9.91FIKK45 pKa = 9.76ICRR48 pKa = 11.84EE49 pKa = 3.71LHH51 pKa = 6.27EE52 pKa = 5.93DD53 pKa = 4.19GSPHH57 pKa = 5.9IHH59 pKa = 6.36VLIQFEE65 pKa = 5.43GKK67 pKa = 9.21FQCKK71 pKa = 8.51NNRR74 pKa = 11.84FFDD77 pKa = 3.93LVSPSRR83 pKa = 11.84SAHH86 pKa = 4.9FHH88 pKa = 6.45PNIQGAKK95 pKa = 8.99SASDD99 pKa = 3.16VKK101 pKa = 11.13AYY103 pKa = 9.63IDD105 pKa = 3.49KK106 pKa = 11.26DD107 pKa = 3.58GDD109 pKa = 3.84VVEE112 pKa = 4.67WGVFQIDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGQKK128 pKa = 8.47TANDD132 pKa = 4.16AYY134 pKa = 10.58AQAINTGNKK143 pKa = 9.54DD144 pKa = 3.43DD145 pKa = 4.06AFKK148 pKa = 10.94VLKK151 pKa = 10.35EE152 pKa = 4.17FAPKK156 pKa = 10.28DD157 pKa = 3.64YY158 pKa = 11.17VLQFHH163 pKa = 6.59NLITNLDD170 pKa = 4.15RR171 pKa = 11.84IFQPRR176 pKa = 11.84SEE178 pKa = 4.54IYY180 pKa = 10.3VSPFSILSFDD190 pKa = 3.63RR191 pKa = 11.84VPPEE195 pKa = 3.62LVDD198 pKa = 3.41WVSSNVVCAAARR210 pKa = 11.84PFRR213 pKa = 11.84PISVVIEE220 pKa = 4.03GDD222 pKa = 3.38SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84CLGPHH237 pKa = 6.84NYY239 pKa = 10.25LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.15VYY251 pKa = 11.07SNDD254 pKa = 2.25AWYY257 pKa = 10.92NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.47YY287 pKa = 10.28GKK289 pKa = 9.35PVMIKK294 pKa = 10.55GGIPTIFLCNKK305 pKa = 9.29GPNSSYY311 pKa = 11.07KK312 pKa = 10.45EE313 pKa = 3.93YY314 pKa = 11.01LDD316 pKa = 3.84EE317 pKa = 4.8EE318 pKa = 4.44KK319 pKa = 10.99NAALKK324 pKa = 9.93QWAIKK329 pKa = 9.99NAVFITLEE337 pKa = 3.92EE338 pKa = 3.96PLYY341 pKa = 10.82SGRR344 pKa = 11.84EE345 pKa = 3.92NIAPPEE351 pKa = 3.97EE352 pKa = 4.3EE353 pKa = 4.39EE354 pKa = 4.22EE355 pKa = 4.28HH356 pKa = 7.87SEE358 pKa = 4.1EE359 pKa = 4.81ASS361 pKa = 3.25

Molecular weight: 41.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2DJ41|D2DJ41_9GEMI AC5 OS=Bhendi yellow vein Haryana virus [2003:Karnal] OX=658650 GN=AC5 PE=4 SV=1
MM1 pKa = 7.9AKK3 pKa = 9.82RR4 pKa = 11.84AADD7 pKa = 3.46IVISTPASKK16 pKa = 10.29VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFGSPYY27 pKa = 7.94TSRR30 pKa = 11.84VAAPIVRR37 pKa = 11.84VTKK40 pKa = 9.91QQAWTNRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 7.8PRR54 pKa = 11.84MYY56 pKa = 10.68RR57 pKa = 11.84MYY59 pKa = 10.44RR60 pKa = 11.84SPDD63 pKa = 3.21VPRR66 pKa = 11.84GCEE69 pKa = 4.34GPCKK73 pKa = 10.01VQSFEE78 pKa = 4.02SRR80 pKa = 11.84HH81 pKa = 5.54DD82 pKa = 3.51VVHH85 pKa = 6.75IGKK88 pKa = 9.41VMCISDD94 pKa = 3.53VTRR97 pKa = 11.84GVGLTHH103 pKa = 7.14RR104 pKa = 11.84VGKK107 pKa = 9.66RR108 pKa = 11.84FCVKK112 pKa = 9.94SVYY115 pKa = 10.52VLGKK119 pKa = 9.41IWMDD123 pKa = 3.35EE124 pKa = 4.04NIKK127 pKa = 9.32TKK129 pKa = 10.6NHH131 pKa = 5.78TNSVMFFLVRR141 pKa = 11.84DD142 pKa = 3.84RR143 pKa = 11.84RR144 pKa = 11.84PVDD147 pKa = 3.13KK148 pKa = 10.28PQDD151 pKa = 3.56FGEE154 pKa = 4.33VFNMFDD160 pKa = 4.29NEE162 pKa = 4.11PSTATVKK169 pKa = 10.61NMHH172 pKa = 7.0RR173 pKa = 11.84DD174 pKa = 3.31RR175 pKa = 11.84YY176 pKa = 8.78QVLRR180 pKa = 11.84KK181 pKa = 8.43WHH183 pKa = 5.58ATVTGGQYY191 pKa = 10.85ASKK194 pKa = 10.24EE195 pKa = 3.73QALVKK200 pKa = 10.68KK201 pKa = 9.39FVRR204 pKa = 11.84VNNYY208 pKa = 7.82VVYY211 pKa = 9.96NQQEE215 pKa = 3.8AGKK218 pKa = 10.1YY219 pKa = 8.23EE220 pKa = 4.02NHH222 pKa = 6.47TEE224 pKa = 3.99NALMLYY230 pKa = 7.52MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.64FYY251 pKa = 11.34DD252 pKa = 3.32SVTNN256 pKa = 3.98

Molecular weight: 29.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1239

97

361

177.0

20.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.6 ± 1.016

2.26 ± 0.399

4.681 ± 0.457

4.197 ± 0.787

4.439 ± 0.406

4.6 ± 0.278

4.036 ± 0.431

5.488 ± 0.789

6.618 ± 0.461

7.667 ± 1.415

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.663 ± 0.803

5.65 ± 0.426

5.327 ± 0.67

4.6 ± 0.648

6.457 ± 1.071

8.152 ± 1.19

5.811 ± 0.901

7.506 ± 1.188

1.291 ± 0.117

3.955 ± 0.544
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski