Streptomyces viridochromogenes (strain DSM 40736 / JCM 4977 / BCRC 1201 / Tue 494)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces viridochromogenes

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7706 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D9X1L9|D9X1L9_STRVT Malto-oligosyltrehalose synthase OS=Streptomyces viridochromogenes (strain DSM 40736 / JCM 4977 / BCRC 1201 / Tue 494) OX=591159 GN=SSQG_06196 PE=4 SV=1
MM1 pKa = 7.27TSAANSPSGDD11 pKa = 3.52DD12 pKa = 3.92RR13 pKa = 11.84SPSGAGYY20 pKa = 10.64DD21 pKa = 3.81GTDD24 pKa = 3.69DD25 pKa = 3.93SSTPGDD31 pKa = 3.47LALSGTLMIVYY42 pKa = 10.27GLFAVFEE49 pKa = 4.76GIVAIANDD57 pKa = 3.69EE58 pKa = 4.54VYY60 pKa = 10.56TSFGDD65 pKa = 3.8YY66 pKa = 10.63VFEE69 pKa = 5.17FDD71 pKa = 3.26LTAWGWIHH79 pKa = 7.03LVIGVLAVAAGFGLFTAEE97 pKa = 3.29PWARR101 pKa = 11.84IVAAVVVGLALIANFIWLPYY121 pKa = 9.25QPFWSIIMIVTGLFVLWSVFNYY143 pKa = 9.88RR144 pKa = 11.84SSRR147 pKa = 11.84TAA149 pKa = 3.03

Molecular weight: 15.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D9X402|D9X402_STRVT Uncharacterized protein OS=Streptomyces viridochromogenes (strain DSM 40736 / JCM 4977 / BCRC 1201 / Tue 494) OX=591159 GN=SSQG_04330 PE=4 SV=1
MM1 pKa = 7.71SSRR4 pKa = 11.84TRR6 pKa = 11.84TRR8 pKa = 11.84ASKK11 pKa = 10.21RR12 pKa = 11.84AAGVHH17 pKa = 5.1TVQIPRR23 pKa = 11.84QRR25 pKa = 11.84GRR27 pKa = 11.84RR28 pKa = 11.84GAQPFVVVVPEE39 pKa = 4.29RR40 pKa = 11.84PSLTRR45 pKa = 11.84EE46 pKa = 3.72AFGFLARR53 pKa = 11.84LLWKK57 pKa = 9.9HH58 pKa = 5.59RR59 pKa = 11.84RR60 pKa = 11.84AMAPLTLATVALPVTALLHH79 pKa = 5.25WWAWWSGLLLAPLAVAPLTWLAFALLRR106 pKa = 11.84RR107 pKa = 11.84RR108 pKa = 11.84AGRR111 pKa = 11.84SVLLWRR117 pKa = 11.84IGLATGGTSALAWLALAAGFGPTAGPLPVLWLLVTLAVQTAWLAIRR163 pKa = 11.84RR164 pKa = 11.84KK165 pKa = 9.99GG166 pKa = 3.36

Molecular weight: 18.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7706

0

7706

2496427

32

3604

324.0

34.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.161 ± 0.043

0.817 ± 0.008

5.989 ± 0.024

5.783 ± 0.026

2.722 ± 0.019

9.486 ± 0.023

2.331 ± 0.015

3.08 ± 0.018

2.232 ± 0.021

10.222 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.763 ± 0.011

1.776 ± 0.017

6.18 ± 0.025

2.78 ± 0.014

8.291 ± 0.034

5.125 ± 0.018

6.106 ± 0.02

8.506 ± 0.03

1.537 ± 0.012

2.114 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski