Cognatishimia activa

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Cognatishimia

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3406 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P1ITP4|A0A0P1ITP4_9RHOB General stress protein 69 OS=Cognatishimia activa OX=1715691 GN=yhdN_2 PE=4 SV=1
MM1 pKa = 7.74RR2 pKa = 11.84LHH4 pKa = 5.87VAGFAALYY12 pKa = 10.07LVASCASPEE21 pKa = 4.05PEE23 pKa = 5.58AITTEE28 pKa = 3.68PRR30 pKa = 11.84YY31 pKa = 10.71DD32 pKa = 2.93KK33 pKa = 10.93FGNYY37 pKa = 7.71EE38 pKa = 4.12CEE40 pKa = 4.41VIPGTNTCVPDD51 pKa = 4.94DD52 pKa = 5.53DD53 pKa = 5.46EE54 pKa = 5.58DD55 pKa = 4.21PCILPDD61 pKa = 4.36GSVAPPGVEE70 pKa = 3.88CPPPEE75 pKa = 6.07RR76 pKa = 11.84GDD78 pKa = 4.66DD79 pKa = 4.09DD80 pKa = 6.11DD81 pKa = 6.54DD82 pKa = 5.48SSTTGGGRR90 pKa = 11.84PSTPGTGARR99 pKa = 11.84PP100 pKa = 3.34

Molecular weight: 10.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N7MBH6|A0A0N7MBH6_9RHOB ATP-dependent RNA helicase RhlE OS=Cognatishimia activa OX=1715691 GN=rhlE_1 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.81IINARR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.34SLSAA44 pKa = 3.83

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3406

0

3406

1051404

30

2439

308.7

33.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.185 ± 0.053

0.894 ± 0.014

6.11 ± 0.038

6.315 ± 0.038

4.027 ± 0.03

8.249 ± 0.037

2.023 ± 0.021

5.532 ± 0.029

4.029 ± 0.033

9.818 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.841 ± 0.022

3.078 ± 0.021

4.688 ± 0.022

3.396 ± 0.02

5.859 ± 0.039

5.6 ± 0.029

5.415 ± 0.026

7.28 ± 0.033

1.372 ± 0.016

2.289 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski