Bosea sp. PAMC 26642

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Boseaceae; Bosea; unclassified Bosea

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5070 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126NY91|A0A126NY91_9BRAD ABC transmembrane type-1 domain-containing protein OS=Bosea sp. PAMC 26642 OX=1792307 GN=AXW83_15745 PE=3 SV=1
MM1 pKa = 7.42TYY3 pKa = 10.78VVTDD7 pKa = 3.21NCIKK11 pKa = 10.2CKK13 pKa = 10.77YY14 pKa = 7.75MDD16 pKa = 4.28CVEE19 pKa = 4.32VCPVDD24 pKa = 3.79CFYY27 pKa = 11.19EE28 pKa = 4.58GEE30 pKa = 4.21NMLVIHH36 pKa = 7.24PDD38 pKa = 2.97EE39 pKa = 5.88CIDD42 pKa = 4.44CGVCEE47 pKa = 4.44PEE49 pKa = 5.05CPAEE53 pKa = 4.42AIKK56 pKa = 10.65PDD58 pKa = 3.95TEE60 pKa = 4.79PGLEE64 pKa = 3.97SWLQLNSKK72 pKa = 9.32YY73 pKa = 10.7ASSWPNITQKK83 pKa = 10.99KK84 pKa = 9.24DD85 pKa = 3.08APADD89 pKa = 3.53AKK91 pKa = 11.35SFDD94 pKa = 3.68GVAGKK99 pKa = 10.65LEE101 pKa = 4.1AHH103 pKa = 6.91FSPEE107 pKa = 4.01PGEE110 pKa = 4.34GDD112 pKa = 3.12

Molecular weight: 12.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126P745|A0A126P745_9BRAD Alpha/beta hydrolase OS=Bosea sp. PAMC 26642 OX=1792307 GN=AXW83_17570 PE=4 SV=1
MM1 pKa = 7.7LILRR5 pKa = 11.84SLLFNILFYY14 pKa = 11.43LNLALWLVFAAFPALLLPRR33 pKa = 11.84RR34 pKa = 11.84YY35 pKa = 10.19LLVVTRR41 pKa = 11.84GWSRR45 pKa = 11.84STLWLMRR52 pKa = 11.84VVAGISCEE60 pKa = 3.89IKK62 pKa = 10.58GRR64 pKa = 11.84EE65 pKa = 3.92HH66 pKa = 6.61VPTGGVMIASKK77 pKa = 10.02HH78 pKa = 4.21QSSWEE83 pKa = 4.15TFVLVALFPEE93 pKa = 4.57PVFILKK99 pKa = 10.25RR100 pKa = 11.84EE101 pKa = 4.28LTWVPFFGWCLIKK114 pKa = 10.8LGMIPVDD121 pKa = 2.93RR122 pKa = 11.84GGRR125 pKa = 11.84ARR127 pKa = 11.84ALSQVTRR134 pKa = 11.84RR135 pKa = 11.84AKK137 pKa = 10.85VEE139 pKa = 3.82VGQKK143 pKa = 9.84GRR145 pKa = 11.84QLLIFPEE152 pKa = 4.81GTRR155 pKa = 11.84TAPGAPRR162 pKa = 11.84SYY164 pKa = 10.92KK165 pKa = 10.41FGVAHH170 pKa = 7.42IYY172 pKa = 11.05GEE174 pKa = 4.21LGVPCVPVALNSGLYY189 pKa = 7.65WPRR192 pKa = 11.84RR193 pKa = 11.84KK194 pKa = 9.43FLRR197 pKa = 11.84RR198 pKa = 11.84PGTIRR203 pKa = 11.84LEE205 pKa = 4.03FLPAIAPGLGKK216 pKa = 10.24AAFQKK221 pKa = 10.48ILEE224 pKa = 4.32EE225 pKa = 4.17QIEE228 pKa = 4.42TASDD232 pKa = 3.4RR233 pKa = 11.84LLTQGRR239 pKa = 11.84VEE241 pKa = 4.03LALLGLDD248 pKa = 4.2PLAEE252 pKa = 4.43PADD255 pKa = 3.91TTRR258 pKa = 11.84QEE260 pKa = 3.94PRR262 pKa = 11.84PP263 pKa = 3.8

Molecular weight: 29.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5070

0

5070

1560634

41

5926

307.8

33.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.26 ± 0.05

0.792 ± 0.009

5.482 ± 0.027

5.445 ± 0.028

3.675 ± 0.022

8.725 ± 0.032

1.9 ± 0.018

5.276 ± 0.025

3.227 ± 0.03

10.388 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.433 ± 0.019

2.336 ± 0.021

5.322 ± 0.025

3.065 ± 0.017

7.215 ± 0.034

5.357 ± 0.025

5.278 ± 0.027

7.515 ± 0.027

1.288 ± 0.014

2.021 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski