Amino acid dipepetide frequency for Mycobacterium phage Poenanya

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.68AlaAla: 13.68 ± 1.429
0.969AlaCys: 0.969 ± 0.274
6.248AlaAsp: 6.248 ± 0.596
6.84AlaGlu: 6.84 ± 0.512
2.855AlaPhe: 2.855 ± 0.382
10.233AlaGly: 10.233 ± 1.165
2.154AlaHis: 2.154 ± 0.365
4.147AlaIle: 4.147 ± 0.541
4.793AlaLys: 4.793 ± 0.487
7.217AlaLeu: 7.217 ± 0.658
2.585AlaMet: 2.585 ± 0.394
2.585AlaAsn: 2.585 ± 0.396
5.063AlaPro: 5.063 ± 0.647
3.178AlaGln: 3.178 ± 0.481
6.948AlaArg: 6.948 ± 0.626
5.224AlaSer: 5.224 ± 0.662
5.871AlaThr: 5.871 ± 0.595
6.894AlaVal: 6.894 ± 0.585
2.747AlaTrp: 2.747 ± 0.411
2.585AlaTyr: 2.585 ± 0.375
0.0AlaXaa: 0.0 ± 0.0
Cys
0.7CysAla: 0.7 ± 0.227
0.054CysCys: 0.054 ± 0.055
1.4CysAsp: 1.4 ± 0.339
0.969CysGlu: 0.969 ± 0.262
0.269CysPhe: 0.269 ± 0.14
2.047CysGly: 2.047 ± 0.449
0.215CysHis: 0.215 ± 0.118
0.215CysIle: 0.215 ± 0.109
0.323CysLys: 0.323 ± 0.141
0.808CysLeu: 0.808 ± 0.288
0.215CysMet: 0.215 ± 0.104
0.323CysAsn: 0.323 ± 0.144
0.969CysPro: 0.969 ± 0.215
0.269CysGln: 0.269 ± 0.126
0.754CysArg: 0.754 ± 0.252
0.7CysSer: 0.7 ± 0.248
0.754CysThr: 0.754 ± 0.198
0.7CysVal: 0.7 ± 0.174
0.269CysTrp: 0.269 ± 0.115
0.215CysTyr: 0.215 ± 0.11
0.0CysXaa: 0.0 ± 0.0
Asp
6.571AspAla: 6.571 ± 0.55
0.916AspCys: 0.916 ± 0.22
4.524AspAsp: 4.524 ± 0.516
3.124AspGlu: 3.124 ± 0.487
1.831AspPhe: 1.831 ± 0.283
6.571AspGly: 6.571 ± 0.654
1.616AspHis: 1.616 ± 0.336
2.154AspIle: 2.154 ± 0.359
1.777AspLys: 1.777 ± 0.319
5.332AspLeu: 5.332 ± 0.518
1.185AspMet: 1.185 ± 0.293
1.885AspAsn: 1.885 ± 0.357
4.955AspPro: 4.955 ± 0.554
2.424AspGln: 2.424 ± 0.347
5.063AspArg: 5.063 ± 0.65
3.609AspSer: 3.609 ± 0.606
4.201AspThr: 4.201 ± 0.507
4.416AspVal: 4.416 ± 0.549
1.831AspTrp: 1.831 ± 0.351
2.154AspTyr: 2.154 ± 0.278
0.0AspXaa: 0.0 ± 0.0
Glu
5.978GluAla: 5.978 ± 0.641
0.969GluCys: 0.969 ± 0.249
2.639GluAsp: 2.639 ± 0.361
3.07GluGlu: 3.07 ± 0.558
2.316GluPhe: 2.316 ± 0.314
3.178GluGly: 3.178 ± 0.45
1.885GluHis: 1.885 ± 0.402
2.101GluIle: 2.101 ± 0.335
1.885GluLys: 1.885 ± 0.242
5.924GluLeu: 5.924 ± 0.608
1.562GluMet: 1.562 ± 0.283
2.101GluAsn: 2.101 ± 0.326
2.962GluPro: 2.962 ± 0.416
2.801GluGln: 2.801 ± 0.395
4.793GluArg: 4.793 ± 0.626
3.124GluSer: 3.124 ± 0.388
4.632GluThr: 4.632 ± 0.601
3.878GluVal: 3.878 ± 0.466
1.239GluTrp: 1.239 ± 0.256
1.777GluTyr: 1.777 ± 0.327
0.0GluXaa: 0.0 ± 0.0
Phe
3.339PheAla: 3.339 ± 0.435
0.377PheCys: 0.377 ± 0.147
2.37PheAsp: 2.37 ± 0.436
1.67PheGlu: 1.67 ± 0.31
1.077PhePhe: 1.077 ± 0.269
3.285PheGly: 3.285 ± 0.669
0.323PheHis: 0.323 ± 0.134
1.4PheIle: 1.4 ± 0.306
1.077PheLys: 1.077 ± 0.287
1.939PheLeu: 1.939 ± 0.316
0.808PheMet: 0.808 ± 0.225
1.239PheAsn: 1.239 ± 0.297
1.67PhePro: 1.67 ± 0.362
1.131PheGln: 1.131 ± 0.322
1.562PheArg: 1.562 ± 0.316
1.508PheSer: 1.508 ± 0.247
2.101PheThr: 2.101 ± 0.381
1.939PheVal: 1.939 ± 0.248
0.646PheTrp: 0.646 ± 0.157
1.077PheTyr: 1.077 ± 0.244
0.0PheXaa: 0.0 ± 0.0
Gly
8.779GlyAla: 8.779 ± 0.953
1.185GlyCys: 1.185 ± 0.278
6.463GlyAsp: 6.463 ± 0.642
4.255GlyGlu: 4.255 ± 0.519
2.908GlyPhe: 2.908 ± 0.449
10.61GlyGly: 10.61 ± 1.709
2.154GlyHis: 2.154 ± 0.344
3.609GlyIle: 3.609 ± 0.494
2.531GlyLys: 2.531 ± 0.406
6.14GlyLeu: 6.14 ± 0.605
2.424GlyMet: 2.424 ± 0.425
3.124GlyAsn: 3.124 ± 0.332
3.932GlyPro: 3.932 ± 0.493
2.531GlyGln: 2.531 ± 0.627
5.278GlyArg: 5.278 ± 0.55
5.817GlySer: 5.817 ± 0.658
6.625GlyThr: 6.625 ± 0.812
6.355GlyVal: 6.355 ± 0.619
2.693GlyTrp: 2.693 ± 0.45
2.047GlyTyr: 2.047 ± 0.328
0.0GlyXaa: 0.0 ± 0.0
His
1.508HisAla: 1.508 ± 0.306
0.431HisCys: 0.431 ± 0.22
1.131HisAsp: 1.131 ± 0.228
1.508HisGlu: 1.508 ± 0.319
0.431HisPhe: 0.431 ± 0.133
1.885HisGly: 1.885 ± 0.28
0.646HisHis: 0.646 ± 0.193
1.454HisIle: 1.454 ± 0.301
0.862HisLys: 0.862 ± 0.279
1.67HisLeu: 1.67 ± 0.306
0.431HisMet: 0.431 ± 0.136
0.916HisAsn: 0.916 ± 0.223
1.885HisPro: 1.885 ± 0.374
0.754HisGln: 0.754 ± 0.198
2.047HisArg: 2.047 ± 0.398
0.916HisSer: 0.916 ± 0.234
1.4HisThr: 1.4 ± 0.363
1.239HisVal: 1.239 ± 0.258
0.539HisTrp: 0.539 ± 0.154
0.7HisTyr: 0.7 ± 0.174
0.0HisXaa: 0.0 ± 0.0
Ile
5.224IleAla: 5.224 ± 0.491
0.808IleCys: 0.808 ± 0.279
3.609IleAsp: 3.609 ± 0.542
3.339IleGlu: 3.339 ± 0.37
0.7IlePhe: 0.7 ± 0.222
4.039IleGly: 4.039 ± 0.437
1.4IleHis: 1.4 ± 0.294
1.454IleIle: 1.454 ± 0.274
1.346IleLys: 1.346 ± 0.283
2.316IleLeu: 2.316 ± 0.351
0.162IleMet: 0.162 ± 0.084
2.101IleAsn: 2.101 ± 0.305
2.424IlePro: 2.424 ± 0.333
1.077IleGln: 1.077 ± 0.22
2.801IleArg: 2.801 ± 0.407
2.208IleSer: 2.208 ± 0.376
3.447IleThr: 3.447 ± 0.437
3.178IleVal: 3.178 ± 0.379
1.023IleTrp: 1.023 ± 0.272
0.808IleTyr: 0.808 ± 0.214
0.0IleXaa: 0.0 ± 0.0
Lys
3.932LysAla: 3.932 ± 0.49
0.431LysCys: 0.431 ± 0.185
1.885LysAsp: 1.885 ± 0.309
1.562LysGlu: 1.562 ± 0.288
1.239LysPhe: 1.239 ± 0.197
2.531LysGly: 2.531 ± 0.382
0.862LysHis: 0.862 ± 0.25
0.862LysIle: 0.862 ± 0.249
1.67LysLys: 1.67 ± 0.436
2.639LysLeu: 2.639 ± 0.547
0.377LysMet: 0.377 ± 0.123
1.239LysAsn: 1.239 ± 0.288
2.208LysPro: 2.208 ± 0.367
1.346LysGln: 1.346 ± 0.207
2.639LysArg: 2.639 ± 0.452
2.154LysSer: 2.154 ± 0.381
2.316LysThr: 2.316 ± 0.426
2.531LysVal: 2.531 ± 0.352
0.862LysTrp: 0.862 ± 0.259
1.023LysTyr: 1.023 ± 0.259
0.0LysXaa: 0.0 ± 0.0
Leu
7.486LeuAla: 7.486 ± 0.892
0.754LeuCys: 0.754 ± 0.197
5.063LeuAsp: 5.063 ± 0.499
3.878LeuGlu: 3.878 ± 0.419
2.478LeuPhe: 2.478 ± 0.282
5.063LeuGly: 5.063 ± 0.498
1.023LeuHis: 1.023 ± 0.2
3.016LeuIle: 3.016 ± 0.41
1.939LeuLys: 1.939 ± 0.347
5.386LeuLeu: 5.386 ± 0.595
1.777LeuMet: 1.777 ± 0.361
2.37LeuAsn: 2.37 ± 0.384
5.332LeuPro: 5.332 ± 0.759
2.855LeuGln: 2.855 ± 0.424
5.17LeuArg: 5.17 ± 0.622
5.224LeuSer: 5.224 ± 0.563
5.278LeuThr: 5.278 ± 0.576
5.063LeuVal: 5.063 ± 0.59
1.346LeuTrp: 1.346 ± 0.312
2.316LeuTyr: 2.316 ± 0.446
0.0LeuXaa: 0.0 ± 0.0
Met
2.154MetAla: 2.154 ± 0.359
0.269MetCys: 0.269 ± 0.151
1.293MetAsp: 1.293 ± 0.313
0.969MetGlu: 0.969 ± 0.204
0.7MetPhe: 0.7 ± 0.212
2.154MetGly: 2.154 ± 0.322
0.162MetHis: 0.162 ± 0.1
0.969MetIle: 0.969 ± 0.235
1.077MetLys: 1.077 ± 0.266
1.562MetLeu: 1.562 ± 0.267
0.539MetMet: 0.539 ± 0.22
1.131MetAsn: 1.131 ± 0.244
1.185MetPro: 1.185 ± 0.296
0.485MetGln: 0.485 ± 0.168
1.4MetArg: 1.4 ± 0.266
2.747MetSer: 2.747 ± 0.368
1.939MetThr: 1.939 ± 0.281
1.4MetVal: 1.4 ± 0.326
0.215MetTrp: 0.215 ± 0.093
0.485MetTyr: 0.485 ± 0.174
0.0MetXaa: 0.0 ± 0.0
Asn
3.609AsnAla: 3.609 ± 0.42
0.162AsnCys: 0.162 ± 0.089
1.885AsnAsp: 1.885 ± 0.318
2.047AsnGlu: 2.047 ± 0.348
0.808AsnPhe: 0.808 ± 0.269
4.416AsnGly: 4.416 ± 0.6
0.862AsnHis: 0.862 ± 0.166
1.885AsnIle: 1.885 ± 0.383
1.077AsnLys: 1.077 ± 0.241
2.208AsnLeu: 2.208 ± 0.371
0.646AsnMet: 0.646 ± 0.183
1.508AsnAsn: 1.508 ± 0.329
2.531AsnPro: 2.531 ± 0.334
1.131AsnGln: 1.131 ± 0.323
2.047AsnArg: 2.047 ± 0.391
1.723AsnSer: 1.723 ± 0.32
2.101AsnThr: 2.101 ± 0.269
1.67AsnVal: 1.67 ± 0.313
0.7AsnTrp: 0.7 ± 0.195
0.862AsnTyr: 0.862 ± 0.178
0.0AsnXaa: 0.0 ± 0.0
Pro
5.224ProAla: 5.224 ± 0.63
0.539ProCys: 0.539 ± 0.194
4.74ProAsp: 4.74 ± 0.455
4.201ProGlu: 4.201 ± 0.514
1.723ProPhe: 1.723 ± 0.289
6.032ProGly: 6.032 ± 0.64
1.454ProHis: 1.454 ± 0.245
2.047ProIle: 2.047 ± 0.325
2.316ProLys: 2.316 ± 0.378
4.309ProLeu: 4.309 ± 0.536
1.4ProMet: 1.4 ± 0.334
2.101ProAsn: 2.101 ± 0.349
4.039ProPro: 4.039 ± 0.562
2.047ProGln: 2.047 ± 0.362
3.124ProArg: 3.124 ± 0.459
3.285ProSer: 3.285 ± 0.454
3.285ProThr: 3.285 ± 0.475
4.632ProVal: 4.632 ± 0.533
1.239ProTrp: 1.239 ± 0.256
1.939ProTyr: 1.939 ± 0.329
0.0ProXaa: 0.0 ± 0.0
Gln
4.201GlnAla: 4.201 ± 0.629
0.431GlnCys: 0.431 ± 0.231
1.454GlnAsp: 1.454 ± 0.241
1.777GlnGlu: 1.777 ± 0.306
1.346GlnPhe: 1.346 ± 0.236
2.101GlnGly: 2.101 ± 0.403
0.862GlnHis: 0.862 ± 0.214
1.67GlnIle: 1.67 ± 0.297
1.293GlnLys: 1.293 ± 0.232
3.07GlnLeu: 3.07 ± 0.395
0.916GlnMet: 0.916 ± 0.188
0.916GlnAsn: 0.916 ± 0.226
2.478GlnPro: 2.478 ± 0.394
1.562GlnGln: 1.562 ± 0.297
2.531GlnArg: 2.531 ± 0.37
2.154GlnSer: 2.154 ± 0.35
1.723GlnThr: 1.723 ± 0.308
2.154GlnVal: 2.154 ± 0.311
0.539GlnTrp: 0.539 ± 0.159
0.969GlnTyr: 0.969 ± 0.25
0.0GlnXaa: 0.0 ± 0.0
Arg
6.625ArgAla: 6.625 ± 0.627
0.862ArgCys: 0.862 ± 0.259
4.524ArgAsp: 4.524 ± 0.552
4.793ArgGlu: 4.793 ± 0.642
1.885ArgPhe: 1.885 ± 0.359
3.878ArgGly: 3.878 ± 0.39
1.723ArgHis: 1.723 ± 0.382
4.201ArgIle: 4.201 ± 0.564
2.262ArgLys: 2.262 ± 0.332
4.578ArgLeu: 4.578 ± 0.618
2.855ArgMet: 2.855 ± 0.342
2.585ArgAsn: 2.585 ± 0.441
3.393ArgPro: 3.393 ± 0.428
2.316ArgGln: 2.316 ± 0.468
5.709ArgArg: 5.709 ± 0.756
3.501ArgSer: 3.501 ± 0.367
3.501ArgThr: 3.501 ± 0.437
5.332ArgVal: 5.332 ± 0.632
2.478ArgTrp: 2.478 ± 0.367
2.154ArgTyr: 2.154 ± 0.361
0.0ArgXaa: 0.0 ± 0.0
Ser
5.924SerAla: 5.924 ± 0.725
0.377SerCys: 0.377 ± 0.173
4.039SerAsp: 4.039 ± 0.431
3.339SerGlu: 3.339 ± 0.438
1.831SerPhe: 1.831 ± 0.427
6.625SerGly: 6.625 ± 0.817
1.185SerHis: 1.185 ± 0.262
2.962SerIle: 2.962 ± 0.432
2.154SerLys: 2.154 ± 0.346
3.932SerLeu: 3.932 ± 0.429
1.454SerMet: 1.454 ± 0.264
1.993SerAsn: 1.993 ± 0.383
3.07SerPro: 3.07 ± 0.355
1.723SerGln: 1.723 ± 0.264
3.986SerArg: 3.986 ± 0.457
3.824SerSer: 3.824 ± 0.596
3.662SerThr: 3.662 ± 0.5
4.309SerVal: 4.309 ± 0.556
1.239SerTrp: 1.239 ± 0.256
1.346SerTyr: 1.346 ± 0.264
0.0SerXaa: 0.0 ± 0.0
Thr
6.086ThrAla: 6.086 ± 0.632
0.539ThrCys: 0.539 ± 0.195
3.932ThrAsp: 3.932 ± 0.588
3.986ThrGlu: 3.986 ± 0.378
2.208ThrPhe: 2.208 ± 0.354
6.14ThrGly: 6.14 ± 0.643
1.454ThrHis: 1.454 ± 0.357
3.932ThrIle: 3.932 ± 0.508
1.939ThrLys: 1.939 ± 0.276
4.686ThrLeu: 4.686 ± 0.59
1.077ThrMet: 1.077 ± 0.253
1.723ThrAsn: 1.723 ± 0.364
4.578ThrPro: 4.578 ± 0.593
1.831ThrGln: 1.831 ± 0.261
4.255ThrArg: 4.255 ± 0.434
4.039ThrSer: 4.039 ± 0.442
4.309ThrThr: 4.309 ± 0.63
5.709ThrVal: 5.709 ± 0.575
1.293ThrTrp: 1.293 ± 0.298
1.723ThrTyr: 1.723 ± 0.284
0.0ThrXaa: 0.0 ± 0.0
Val
7.056ValAla: 7.056 ± 0.525
1.508ValCys: 1.508 ± 0.326
5.817ValAsp: 5.817 ± 0.628
4.578ValGlu: 4.578 ± 0.611
2.478ValPhe: 2.478 ± 0.369
5.278ValGly: 5.278 ± 0.613
1.346ValHis: 1.346 ± 0.256
2.855ValIle: 2.855 ± 0.39
2.37ValLys: 2.37 ± 0.399
5.063ValLeu: 5.063 ± 0.566
1.293ValMet: 1.293 ± 0.209
2.37ValAsn: 2.37 ± 0.321
4.255ValPro: 4.255 ± 0.464
2.747ValGln: 2.747 ± 0.389
4.524ValArg: 4.524 ± 0.519
4.416ValSer: 4.416 ± 0.606
4.901ValThr: 4.901 ± 0.626
5.978ValVal: 5.978 ± 0.834
1.885ValTrp: 1.885 ± 0.322
1.131ValTyr: 1.131 ± 0.236
0.0ValXaa: 0.0 ± 0.0
Trp
2.101TrpAla: 2.101 ± 0.292
0.215TrpCys: 0.215 ± 0.111
1.508TrpAsp: 1.508 ± 0.299
1.293TrpGlu: 1.293 ± 0.335
0.754TrpPhe: 0.754 ± 0.189
1.023TrpGly: 1.023 ± 0.231
0.539TrpHis: 0.539 ± 0.171
1.293TrpIle: 1.293 ± 0.245
0.646TrpLys: 0.646 ± 0.169
1.885TrpLeu: 1.885 ± 0.329
0.862TrpMet: 0.862 ± 0.271
0.862TrpAsn: 0.862 ± 0.24
1.293TrpPro: 1.293 ± 0.354
1.185TrpGln: 1.185 ± 0.285
2.154TrpArg: 2.154 ± 0.418
1.454TrpSer: 1.454 ± 0.247
1.508TrpThr: 1.508 ± 0.268
2.101TrpVal: 2.101 ± 0.479
0.916TrpTrp: 0.916 ± 0.23
0.7TrpTyr: 0.7 ± 0.185
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.639TyrAla: 2.639 ± 0.412
0.377TyrCys: 0.377 ± 0.154
1.616TyrAsp: 1.616 ± 0.315
1.67TyrGlu: 1.67 ± 0.351
0.7TyrPhe: 0.7 ± 0.195
2.37TyrGly: 2.37 ± 0.38
0.485TyrHis: 0.485 ± 0.139
1.239TyrIle: 1.239 ± 0.228
0.969TyrLys: 0.969 ± 0.246
2.154TyrLeu: 2.154 ± 0.301
0.215TyrMet: 0.215 ± 0.098
0.808TyrAsn: 0.808 ± 0.19
1.454TyrPro: 1.454 ± 0.277
0.754TyrGln: 0.754 ± 0.213
2.37TyrArg: 2.37 ± 0.352
1.346TyrSer: 1.346 ± 0.257
1.885TyrThr: 1.885 ± 0.35
2.37TyrVal: 2.37 ± 0.383
0.592TyrTrp: 0.592 ± 0.189
0.862TyrTyr: 0.862 ± 0.206
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 107 proteins (18568 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski