Pseudomonas phage 8P

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M8EIF9|A0A6M8EIF9_9CAUD Uncharacterized protein OS=Pseudomonas phage 8P OX=2723907 GN=8P_055 PE=4 SV=1
MM1 pKa = 7.67RR2 pKa = 11.84RR3 pKa = 11.84NPDD6 pKa = 2.75AWRR9 pKa = 11.84HH10 pKa = 4.84EE11 pKa = 4.39LDD13 pKa = 4.55PRR15 pKa = 11.84DD16 pKa = 3.98PDD18 pKa = 3.67YY19 pKa = 11.62LEE21 pKa = 4.53PPEE24 pKa = 5.78KK25 pKa = 9.78IEE27 pKa = 5.32EE28 pKa = 4.32DD29 pKa = 3.57DD30 pKa = 4.65QEE32 pKa = 4.99PEE34 pKa = 4.16EE35 pKa = 4.81DD36 pKa = 3.83WPAADD41 pKa = 5.16DD42 pKa = 3.98PRR44 pKa = 11.84GDD46 pKa = 3.64GPYY49 pKa = 10.14HH50 pKa = 6.55YY51 pKa = 9.9IPPYY55 pKa = 10.24EE56 pKa = 3.98PP57 pKa = 4.24

Molecular weight: 6.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M8E0G2|A0A6M8E0G2_9CAUD Uncharacterized protein OS=Pseudomonas phage 8P OX=2723907 GN=8P_019 PE=4 SV=1
MM1 pKa = 7.25TKK3 pKa = 10.19PRR5 pKa = 11.84IDD7 pKa = 4.57SIRR10 pKa = 11.84MTYY13 pKa = 8.45RR14 pKa = 11.84TEE16 pKa = 3.97PPRR19 pKa = 11.84KK20 pKa = 9.16KK21 pKa = 10.03PQAGDD26 pKa = 2.94IRR28 pKa = 11.84VIKK31 pKa = 9.52GKK33 pKa = 8.28TMIRR37 pKa = 11.84QQVRR41 pKa = 11.84HH42 pKa = 5.62NGMYY46 pKa = 9.63VVSGGRR52 pKa = 11.84PVWEE56 pKa = 3.92WVEE59 pKa = 4.01KK60 pKa = 10.55GGEE63 pKa = 3.88RR64 pKa = 11.84DD65 pKa = 3.0RR66 pKa = 11.84TARR69 pKa = 11.84QEE71 pKa = 4.13VKK73 pKa = 9.91TLL75 pKa = 3.37

Molecular weight: 8.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

20304

42

5145

322.3

35.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.776 ± 0.59

0.798 ± 0.242

6.097 ± 0.188

6.91 ± 0.316

3.216 ± 0.183

7.688 ± 0.364

1.98 ± 0.244

4.477 ± 0.158

4.679 ± 0.323

7.462 ± 0.244

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.532 ± 0.117

3.127 ± 0.112

5.595 ± 0.321

4.566 ± 0.257

6.718 ± 0.25

5.216 ± 0.191

5.841 ± 0.228

6.245 ± 0.243

1.414 ± 0.112

2.664 ± 0.269
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski