Trachipleistophora hominis (Microsporidian parasite)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Microsporidia; Pansporoblastina; Pleistophoridae; Trachipleistophora

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3212 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L7JUU9|L7JUU9_TRAHO Putative bZIP transcription factor OS=Trachipleistophora hominis OX=72359 GN=THOM_1906 PE=4 SV=1
MM1 pKa = 7.44NFLEE5 pKa = 4.45YY6 pKa = 10.66LVLEE10 pKa = 4.62CKK12 pKa = 10.02RR13 pKa = 11.84RR14 pKa = 11.84LLSTYY19 pKa = 10.77SSTNNILQEE28 pKa = 3.93PQAAQMNCNVYY39 pKa = 10.44QNEE42 pKa = 4.02NAFRR46 pKa = 11.84RR47 pKa = 11.84EE48 pKa = 3.59MSMVFLMFSVMFIIWFLLMYY68 pKa = 10.21FCLTKK73 pKa = 10.35TPYY76 pKa = 9.53QQPDD80 pKa = 3.16THH82 pKa = 8.01SEE84 pKa = 3.71QEE86 pKa = 4.24YY87 pKa = 9.55VVEE90 pKa = 4.3IGEE93 pKa = 4.36NNSLMSLNSNQEE105 pKa = 3.66NNARR109 pKa = 11.84PQSEE113 pKa = 4.0ASVDD117 pKa = 3.58GHH119 pKa = 6.8HH120 pKa = 6.84VDD122 pKa = 4.74SPLSYY127 pKa = 10.98DD128 pKa = 3.56RR129 pKa = 11.84NNDD132 pKa = 2.87EE133 pKa = 5.34DD134 pKa = 4.73LYY136 pKa = 11.41EE137 pKa = 4.94EE138 pKa = 4.48IPSTEE143 pKa = 3.9SSSFSVFSDD152 pKa = 3.16DD153 pKa = 3.77TRR155 pKa = 11.84SFDD158 pKa = 3.61TDD160 pKa = 3.54SQITPEE166 pKa = 4.14YY167 pKa = 9.29TLLPSPFLDD176 pKa = 3.37IEE178 pKa = 4.34LLLFVHH184 pKa = 6.46NLLDD188 pKa = 3.75RR189 pKa = 11.84TSSSTSADD197 pKa = 3.48DD198 pKa = 5.02DD199 pKa = 3.94NAQITSPRR207 pKa = 11.84IQLIDD212 pKa = 3.93SNVSQLDD219 pKa = 3.24IEE221 pKa = 5.16LNLFLNLQMDD231 pKa = 4.22SDD233 pKa = 4.66DD234 pKa = 4.67LPSNSSSLEE243 pKa = 4.12VINTEE248 pKa = 3.9

Molecular weight: 28.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L7JXT7|L7JXT7_TRAHO Tubulin gamma chain OS=Trachipleistophora hominis OX=72359 GN=THOM_0785 PE=3 SV=1
MM1 pKa = 7.6LSLEE5 pKa = 4.16VLEE8 pKa = 5.37VITGCVWAGMLCMAVKK24 pKa = 10.34VWRR27 pKa = 11.84LLDD30 pKa = 3.93GKK32 pKa = 10.23LDD34 pKa = 4.06CGLVRR39 pKa = 11.84MMGMLILLMLRR50 pKa = 11.84LFMKK54 pKa = 10.19IRR56 pKa = 11.84LEE58 pKa = 4.18GGVGGSRR65 pKa = 11.84DD66 pKa = 3.44VGGDD70 pKa = 3.04RR71 pKa = 11.84DD72 pKa = 3.84GGVGRR77 pKa = 11.84GRR79 pKa = 11.84NGGVWRR85 pKa = 11.84DD86 pKa = 3.37RR87 pKa = 11.84NGGVGRR93 pKa = 11.84DD94 pKa = 3.32RR95 pKa = 11.84NGGVGRR101 pKa = 11.84DD102 pKa = 3.34RR103 pKa = 11.84NGGVWRR109 pKa = 11.84NPLGRR114 pKa = 11.84DD115 pKa = 3.46YY116 pKa = 11.15NTT118 pKa = 3.87

Molecular weight: 12.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3212

0

3212

933265

21

2764

290.6

33.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.404 ± 0.043

2.095 ± 0.021

5.838 ± 0.038

7.13 ± 0.054

4.813 ± 0.039

4.587 ± 0.051

2.078 ± 0.016

7.266 ± 0.052

8.224 ± 0.06

9.11 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.722 ± 0.018

6.622 ± 0.045

2.708 ± 0.031

2.761 ± 0.023

5.32 ± 0.038

6.664 ± 0.043

5.168 ± 0.03

6.672 ± 0.041

0.595 ± 0.011

4.026 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski