Streptomyces thermoautotrophicus

Taxonomy:

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4356 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A132MUB5|A0A132MUB5_9ACTN Allantoate amidohydrolase OS=Streptomyces thermoautotrophicus OX=1469144 GN=LI90_2527 PE=3 SV=1
MM1 pKa = 7.97AEE3 pKa = 4.24PLKK6 pKa = 11.11VWIDD10 pKa = 3.58QNLCTGDD17 pKa = 3.96GLCAQYY23 pKa = 11.26APDD26 pKa = 4.19VFEE29 pKa = 6.18LDD31 pKa = 3.22IDD33 pKa = 3.59GLAYY37 pKa = 10.73VKK39 pKa = 10.94GPDD42 pKa = 3.4GEE44 pKa = 5.34LRR46 pKa = 11.84QQHH49 pKa = 6.23CASVPVPPDD58 pKa = 3.06LVLNVIDD65 pKa = 4.75SAKK68 pKa = 9.65EE69 pKa = 3.86CPGEE73 pKa = 4.35CIHH76 pKa = 6.8VVRR79 pKa = 11.84EE80 pKa = 3.9PDD82 pKa = 3.38GVEE85 pKa = 3.37IAGPNAEE92 pKa = 4.24EE93 pKa = 3.98

Molecular weight: 9.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A132MLI5|A0A132MLI5_9ACTN Uncharacterized protein OS=Streptomyces thermoautotrophicus OX=1469144 GN=LI90_270 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.28THH17 pKa = 5.07GFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.35GRR40 pKa = 11.84QRR42 pKa = 11.84LSAA45 pKa = 4.04

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4356

0

4356

1278468

37

3137

293.5

31.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.134 ± 0.057

0.914 ± 0.011

5.665 ± 0.03

6.267 ± 0.036

2.736 ± 0.022

8.808 ± 0.035

2.243 ± 0.018

3.496 ± 0.026

2.173 ± 0.03

10.566 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.669 ± 0.016

1.733 ± 0.019

6.219 ± 0.039

2.969 ± 0.022

9.025 ± 0.049

4.3 ± 0.019

5.452 ± 0.025

8.861 ± 0.034

1.539 ± 0.017

2.23 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski