Corynebacterium phage Bran

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6ENC9|A0A4Y6ENC9_9CAUD Tail assembly chaperone OS=Corynebacterium phage Bran OX=2588500 GN=13 PE=4 SV=1
MM1 pKa = 7.23SWSPADD7 pKa = 4.75DD8 pKa = 4.76FDD10 pKa = 7.03DD11 pKa = 4.25EE12 pKa = 4.94GNYY15 pKa = 10.75APGPYY20 pKa = 9.71PFRR23 pKa = 11.84PRR25 pKa = 11.84HH26 pKa = 5.56ARR28 pKa = 11.84CQPGCCGIGPRR39 pKa = 11.84LCEE42 pKa = 4.09EE43 pKa = 4.51PRR45 pKa = 11.84DD46 pKa = 3.99PDD48 pKa = 4.61DD49 pKa = 5.38DD50 pKa = 3.94LHH52 pKa = 8.0EE53 pKa = 4.73EE54 pKa = 4.18ADD56 pKa = 3.5QQ57 pKa = 4.17

Molecular weight: 6.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6EP09|A0A4Y6EP09_9CAUD Site-specific DNA-methyltransferase (cytosine-N(4)-specific) OS=Corynebacterium phage Bran OX=2588500 GN=64 PE=3 SV=1
MM1 pKa = 7.31MPTPRR6 pKa = 11.84TPRR9 pKa = 11.84GRR11 pKa = 11.84LLDD14 pKa = 3.7IAALAAVSIWCAQTAAAYY32 pKa = 7.75LVPGGSEE39 pKa = 3.55IHH41 pKa = 5.8QLALAGTLINLKK53 pKa = 9.86AWGVAWIIVAVIVAIGIWSRR73 pKa = 11.84TMRR76 pKa = 11.84IIGLSIFVGMNIMWAASFLEE96 pKa = 4.26AWAVGTSDD104 pKa = 4.47RR105 pKa = 11.84AWVSAKK111 pKa = 10.72NYY113 pKa = 10.56GLIAALGVCVTAWTGGVAHH132 pKa = 6.86RR133 pKa = 11.84HH134 pKa = 5.43DD135 pKa = 4.19LRR137 pKa = 6.08

Molecular weight: 14.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

14176

39

1803

205.4

22.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.655 ± 0.663

0.705 ± 0.135

6.624 ± 0.287

6.391 ± 0.3

2.42 ± 0.218

8.733 ± 0.485

2.3 ± 0.23

4.663 ± 0.203

3.21 ± 0.207

7.738 ± 0.34

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.61 ± 0.106

2.504 ± 0.174

5.848 ± 0.383

3.315 ± 0.169

7.273 ± 0.391

4.952 ± 0.311

6.504 ± 0.285

7.033 ± 0.255

2.328 ± 0.165

2.194 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski