Ostreococcus tauri virus 2

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Prasinovirus; unclassified Prasinovirus

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 237 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|E4WLP8|E4WLP8_9PHYC Asparagine synthase (glutamine-hydrolyzing) OS=Ostreococcus tauri virus 2 OX=696472 GN=OtV2_022 PE=4 SV=1
MM1 pKa = 7.4IVDD4 pKa = 3.97VQCEE8 pKa = 3.97DD9 pKa = 3.69DD10 pKa = 4.21TIQIANLIRR19 pKa = 11.84DD20 pKa = 4.19EE21 pKa = 4.19NTHH24 pKa = 5.57DD25 pKa = 3.53VEE27 pKa = 4.61VRR29 pKa = 11.84FLKK32 pKa = 10.5RR33 pKa = 11.84IKK35 pKa = 10.81SNLYY39 pKa = 10.66DD40 pKa = 3.86FDD42 pKa = 4.93NEE44 pKa = 3.77ISVVPKK50 pKa = 10.36EE51 pKa = 4.74SVCGWYY57 pKa = 10.01DD58 pKa = 3.66CEE60 pKa = 4.13NLEE63 pKa = 4.75DD64 pKa = 3.71TEE66 pKa = 5.76LYY68 pKa = 10.61VKK70 pKa = 10.87VSGGYY75 pKa = 10.44DD76 pKa = 3.22LVDD79 pKa = 4.16DD80 pKa = 5.48SEE82 pKa = 6.45DD83 pKa = 3.58EE84 pKa = 5.27DD85 pKa = 4.64FTCSEE90 pKa = 3.97SDD92 pKa = 3.6EE93 pKa = 4.64PDD95 pKa = 3.54SEE97 pKa = 4.7SEE99 pKa = 4.39SLVHH103 pKa = 6.91EE104 pKa = 4.97DD105 pKa = 3.39EE106 pKa = 4.44AA107 pKa = 6.31

Molecular weight:
12.29 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|E4WM65|E4WM65_9PHYC DUF5824 domain-containing protein OS=Ostreococcus tauri virus 2 OX=696472 GN=OtV2_189 PE=4 SV=1
MM1 pKa = 7.24HH2 pKa = 6.92VKK4 pKa = 10.19ILRR7 pKa = 11.84SPDD10 pKa = 2.97RR11 pKa = 11.84KK12 pKa = 10.34KK13 pKa = 10.71KK14 pKa = 10.13FRR16 pKa = 11.84AILGDD21 pKa = 3.19GRR23 pKa = 11.84TVDD26 pKa = 5.31FGASGYY32 pKa = 10.89SDD34 pKa = 3.53YY35 pKa = 10.9TKK37 pKa = 10.58HH38 pKa = 6.49KK39 pKa = 9.02NPSRR43 pKa = 11.84MRR45 pKa = 11.84SYY47 pKa = 11.14VLRR50 pKa = 11.84HH51 pKa = 6.09GGQIPKK57 pKa = 10.04RR58 pKa = 11.84VVAEE62 pKa = 4.3RR63 pKa = 11.84EE64 pKa = 3.5PAMIHH69 pKa = 6.48RR70 pKa = 11.84MMRR73 pKa = 11.84NIDD76 pKa = 3.27KK77 pKa = 10.62SDD79 pKa = 3.71RR80 pKa = 11.84EE81 pKa = 4.1DD82 pKa = 3.09WKK84 pKa = 11.38LSGIGGAGFWSRR96 pKa = 11.84WYY98 pKa = 10.29LWSQPTIPAVQRR110 pKa = 11.84FMSKK114 pKa = 9.89RR115 pKa = 11.84FGIKK119 pKa = 10.36FII121 pKa = 4.63

Molecular weight:
14.18 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

237

0

237

57106

66

2566

241.0

27.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.852 ± 0.246

1.741 ± 0.149

5.845 ± 0.148

6.738 ± 0.199

4.262 ± 0.145

6.257 ± 0.249

2.208 ± 0.124

6.437 ± 0.165

7.673 ± 0.308

7.961 ± 0.157

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.896 ± 0.109

5.819 ± 0.359

4.087 ± 0.162

3.409 ± 0.117

4.978 ± 0.195

6.141 ± 0.161

6.166 ± 0.236

6.442 ± 0.149

1.086 ± 0.073

3.989 ± 0.159

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski