Cotton leaf curl virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; unclassified Begomovirus

Average proteome isoelectric point is 7.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D8RG60|A0A1D8RG60_9GEMI Regulatory protein OS=Cotton leaf curl virus OX=53010 GN=AC4 PE=3 SV=1
MM1 pKa = 8.06WDD3 pKa = 3.35PLLNEE8 pKa = 4.84FPDD11 pKa = 4.03TVHH14 pKa = 7.02GFRR17 pKa = 11.84CMLSVKK23 pKa = 9.9YY24 pKa = 8.88LQPLSQDD31 pKa = 3.46YY32 pKa = 11.44SPDD35 pKa = 3.3TLGYY39 pKa = 10.31EE40 pKa = 4.86LIRR43 pKa = 11.84DD44 pKa = 4.64LICILRR50 pKa = 11.84SRR52 pKa = 11.84NYY54 pKa = 9.65VEE56 pKa = 4.26ASCRR60 pKa = 11.84YY61 pKa = 7.36RR62 pKa = 11.84HH63 pKa = 6.19FYY65 pKa = 10.95ARR67 pKa = 11.84VEE69 pKa = 4.15STPASEE75 pKa = 4.25LRR77 pKa = 11.84QPIHH81 pKa = 6.33QPCCCPHH88 pKa = 6.61CPRR91 pKa = 11.84HH92 pKa = 5.62KK93 pKa = 8.3TTGMDD98 pKa = 3.07KK99 pKa = 10.37QAYY102 pKa = 6.5EE103 pKa = 4.08QEE105 pKa = 4.32AQDD108 pKa = 3.71VQDD111 pKa = 3.9VQKK114 pKa = 10.9SRR116 pKa = 11.84CSS118 pKa = 3.24

Molecular weight: 13.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q1KSG4|Q1KSG4_9GEMI Transcriptional activator protein OS=Cotton leaf curl virus OX=53010 GN=AC2 PE=3 SV=1
MM1 pKa = 7.56NILHH5 pKa = 6.29SRR7 pKa = 11.84RR8 pKa = 11.84TGLIIKK14 pKa = 8.96HH15 pKa = 5.87IKK17 pKa = 9.33YY18 pKa = 9.9LSKK21 pKa = 10.22ILRR24 pKa = 11.84FINRR28 pKa = 11.84STISNQEE35 pKa = 3.42KK36 pKa = 8.91HH37 pKa = 4.46HH38 pKa = 6.21TIRR41 pKa = 11.84VILRR45 pKa = 11.84LNVLIHH51 pKa = 7.05PYY53 pKa = 8.72LTQHH57 pKa = 6.97INRR60 pKa = 11.84LDD62 pKa = 3.8TKK64 pKa = 11.05SLTNSMGQPSTTSNITNTHH83 pKa = 5.84YY84 pKa = 9.03FTYY87 pKa = 9.5MLNIMSGLKK96 pKa = 10.08RR97 pKa = 11.84LNLTWTFTSSRR108 pKa = 11.84NIWTSVHH115 pKa = 6.21PVHH118 pKa = 7.12PGLPVHH124 pKa = 7.01RR125 pKa = 11.84PVCPCLLFCDD135 pKa = 4.37ADD137 pKa = 3.76NGGSSTAGVWAAEE150 pKa = 4.38VQTPAYY156 pKa = 9.3FRR158 pKa = 11.84RR159 pKa = 11.84GRR161 pKa = 11.84RR162 pKa = 11.84NDD164 pKa = 4.35DD165 pKa = 3.06IGSSLRR171 pKa = 11.84HH172 pKa = 5.69NYY174 pKa = 10.14GSVKK178 pKa = 10.6YY179 pKa = 10.05KK180 pKa = 10.71LNPVLTRR187 pKa = 11.84TQAYY191 pKa = 7.49PVNNPATKK199 pKa = 10.13VANISQKK206 pKa = 10.88AYY208 pKa = 8.81TEE210 pKa = 3.95TRR212 pKa = 11.84EE213 pKa = 4.02PYY215 pKa = 9.73QGIRR219 pKa = 11.84LIVDD223 pKa = 3.73PTCLNLKK230 pKa = 10.38LSAQVLIVCGSVII243 pKa = 4.03

Molecular weight: 27.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1363

100

362

194.7

22.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.842 ± 0.569

2.421 ± 0.605

4.255 ± 0.595

4.255 ± 0.726

3.888 ± 0.556

5.062 ± 0.437

3.742 ± 0.64

6.016 ± 0.715

5.503 ± 0.783

7.704 ± 0.691

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.054 ± 0.354

5.796 ± 0.585

5.649 ± 0.344

5.356 ± 0.664

7.043 ± 0.869

8.657 ± 1.124

6.823 ± 0.944

5.649 ± 1.006

1.394 ± 0.096

3.888 ± 0.374
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski