Wallemia hederae

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Wallemiomycotina; Wallemiomycetes; Wallemiales; Wallemiaceae; Wallemia

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4107 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4T0FCD5|A0A4T0FCD5_9BASI Aminotran_1_2 domain-containing protein OS=Wallemia hederae OX=1540922 GN=E3P99_03898 PE=4 SV=1
MM1 pKa = 7.03VGKK4 pKa = 10.51FGLLSLAAVAGVAAAQSKK22 pKa = 10.17DD23 pKa = 3.49YY24 pKa = 10.82PGVVSANDD32 pKa = 3.59NGPTNPDD39 pKa = 3.2QPEE42 pKa = 4.58LGTDD46 pKa = 3.41TPEE49 pKa = 4.47DD50 pKa = 3.61SEE52 pKa = 5.14ARR54 pKa = 11.84LLTVNNIDD62 pKa = 4.06DD63 pKa = 3.92FCIFGPKK70 pKa = 10.09DD71 pKa = 3.2ADD73 pKa = 3.46KK74 pKa = 10.98TIGEE78 pKa = 4.44TEE80 pKa = 4.03AEE82 pKa = 4.08QVAWCTQPRR91 pKa = 11.84NNARR95 pKa = 11.84LIPEE99 pKa = 3.97DD100 pKa = 4.19TFTGLHH106 pKa = 5.54FVKK109 pKa = 9.94TPYY112 pKa = 10.39YY113 pKa = 9.96IQLQGWGDD121 pKa = 3.56LTKK124 pKa = 10.45IGIKK128 pKa = 10.61DD129 pKa = 3.23GDD131 pKa = 3.9YY132 pKa = 11.17GGEE135 pKa = 4.14LDD137 pKa = 3.57PHH139 pKa = 5.94GAKK142 pKa = 10.57DD143 pKa = 3.69LGNPIGGNVTSNISGEE159 pKa = 3.99ATFYY163 pKa = 10.39EE164 pKa = 4.5EE165 pKa = 3.5WMEE168 pKa = 4.49FISYY172 pKa = 9.4EE173 pKa = 4.14QFCLRR178 pKa = 11.84VCTAGDD184 pKa = 4.06DD185 pKa = 3.68TWDD188 pKa = 3.44AATMCEE194 pKa = 4.08HH195 pKa = 7.14KK196 pKa = 10.25LDD198 pKa = 3.76EE199 pKa = 4.6MGCGFIMPGDD209 pKa = 3.85YY210 pKa = 9.93QGKK213 pKa = 8.18QDD215 pKa = 4.48QFVSCDD221 pKa = 3.08ADD223 pKa = 3.43AAFPPGVYY231 pKa = 9.46PDD233 pKa = 3.71GDD235 pKa = 3.83GYY237 pKa = 10.24STFNQRR243 pKa = 11.84YY244 pKa = 6.64TGTLTSNTYY253 pKa = 9.38EE254 pKa = 4.21VYY256 pKa = 10.05TVGDD260 pKa = 3.78TATPSGPYY268 pKa = 8.6STPSSSNCKK277 pKa = 8.08TQDD280 pKa = 3.37GLNTYY285 pKa = 10.28VNTDD289 pKa = 3.22APQSTGSSDD298 pKa = 3.38NNTNAEE304 pKa = 4.25NGDD307 pKa = 3.81SGAGSVALGAVATVSIALISAFAILSS333 pKa = 3.64

Molecular weight:
35.27 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4T0FKS1|A0A4T0FKS1_9BASI Isoleucyl-tRNA synthetase OS=Wallemia hederae OX=1540922 GN=E3P99_02380 PE=3 SV=1
MM1 pKa = 7.82ASPATVFSPTLNALNQLRR19 pKa = 11.84FASRR23 pKa = 11.84GTDD26 pKa = 3.54YY27 pKa = 11.19QPSTRR32 pKa = 11.84KK33 pKa = 9.59RR34 pKa = 11.84KK35 pKa = 8.51RR36 pKa = 11.84TFGFLARR43 pKa = 11.84IRR45 pKa = 11.84SRR47 pKa = 11.84TGRR50 pKa = 11.84KK51 pKa = 9.05IIARR55 pKa = 11.84RR56 pKa = 11.84LKK58 pKa = 10.34RR59 pKa = 11.84GRR61 pKa = 11.84KK62 pKa = 8.89NMSHH66 pKa = 6.79

Molecular weight:
7.63 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4107

0

4107

2297997

59

4344

559.5

62.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.12 ± 0.031

1.029 ± 0.012

6.308 ± 0.027

6.179 ± 0.034

3.578 ± 0.022

5.863 ± 0.031

2.597 ± 0.017

5.157 ± 0.024

5.862 ± 0.031

9.168 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.056 ± 0.012

4.381 ± 0.02

5.143 ± 0.032

4.548 ± 0.034

5.422 ± 0.025

8.784 ± 0.038

5.748 ± 0.02

6.07 ± 0.028

1.101 ± 0.011

2.887 ± 0.015

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski