Amino acid dipepetide frequency for Freshwater phage uvFW-CGR-AMD-COM-C440

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.708AlaAla: 11.708 ± 2.986
0.571AlaCys: 0.571 ± 0.334
4.759AlaAsp: 4.759 ± 0.694
6.663AlaGlu: 6.663 ± 1.168
2.856AlaPhe: 2.856 ± 0.727
8.471AlaGly: 8.471 ± 1.368
1.333AlaHis: 1.333 ± 0.399
5.616AlaIle: 5.616 ± 0.773
6.663AlaLys: 6.663 ± 0.852
9.328AlaLeu: 9.328 ± 1.069
3.141AlaMet: 3.141 ± 0.467
4.378AlaAsn: 4.378 ± 0.842
2.76AlaPro: 2.76 ± 0.664
5.045AlaGln: 5.045 ± 1.041
5.14AlaArg: 5.14 ± 0.791
8.852AlaSer: 8.852 ± 1.721
7.615AlaThr: 7.615 ± 2.041
6.758AlaVal: 6.758 ± 0.943
0.666AlaTrp: 0.666 ± 0.244
3.236AlaTyr: 3.236 ± 0.797
0.0AlaXaa: 0.0 ± 0.0
Cys
0.857CysAla: 0.857 ± 0.336
0.0CysCys: 0.0 ± 0.0
0.476CysAsp: 0.476 ± 0.203
0.476CysGlu: 0.476 ± 0.238
0.286CysPhe: 0.286 ± 0.17
1.333CysGly: 1.333 ± 0.47
0.19CysHis: 0.19 ± 0.16
0.761CysIle: 0.761 ± 0.323
0.476CysLys: 0.476 ± 0.258
0.476CysLeu: 0.476 ± 0.237
0.0CysMet: 0.0 ± 0.0
0.476CysAsn: 0.476 ± 0.267
0.571CysPro: 0.571 ± 0.233
0.286CysGln: 0.286 ± 0.143
0.286CysArg: 0.286 ± 0.184
0.19CysSer: 0.19 ± 0.131
0.0CysThr: 0.0 ± 0.0
0.19CysVal: 0.19 ± 0.138
0.095CysTrp: 0.095 ± 0.098
0.095CysTyr: 0.095 ± 0.12
0.0CysXaa: 0.0 ± 0.0
Asp
5.235AspAla: 5.235 ± 0.764
0.666AspCys: 0.666 ± 0.251
3.236AspAsp: 3.236 ± 0.752
3.141AspGlu: 3.141 ± 0.57
2.094AspPhe: 2.094 ± 0.358
4.188AspGly: 4.188 ± 0.558
0.571AspHis: 0.571 ± 0.291
3.236AspIle: 3.236 ± 0.495
3.427AspLys: 3.427 ± 0.555
5.616AspLeu: 5.616 ± 0.718
0.857AspMet: 0.857 ± 0.345
3.617AspAsn: 3.617 ± 0.559
3.236AspPro: 3.236 ± 0.619
1.523AspGln: 1.523 ± 0.567
3.236AspArg: 3.236 ± 0.462
3.046AspSer: 3.046 ± 0.507
4.569AspThr: 4.569 ± 0.695
3.141AspVal: 3.141 ± 0.536
0.571AspTrp: 0.571 ± 0.175
2.094AspTyr: 2.094 ± 0.404
0.0AspXaa: 0.0 ± 0.0
Glu
6.568GluAla: 6.568 ± 1.228
0.571GluCys: 0.571 ± 0.283
3.331GluAsp: 3.331 ± 0.698
3.807GluGlu: 3.807 ± 0.77
2.665GluPhe: 2.665 ± 0.603
3.712GluGly: 3.712 ± 0.704
1.523GluHis: 1.523 ± 0.542
3.522GluIle: 3.522 ± 0.584
3.712GluLys: 3.712 ± 0.75
4.95GluLeu: 4.95 ± 0.862
1.523GluMet: 1.523 ± 0.446
2.38GluAsn: 2.38 ± 0.782
1.618GluPro: 1.618 ± 0.375
2.856GluGln: 2.856 ± 0.597
2.856GluArg: 2.856 ± 0.577
2.57GluSer: 2.57 ± 0.474
3.617GluThr: 3.617 ± 0.546
3.712GluVal: 3.712 ± 0.505
0.666GluTrp: 0.666 ± 0.241
1.999GluTyr: 1.999 ± 0.385
0.0GluXaa: 0.0 ± 0.0
Phe
3.236PheAla: 3.236 ± 0.496
0.286PheCys: 0.286 ± 0.161
1.618PheAsp: 1.618 ± 0.336
1.618PheGlu: 1.618 ± 0.393
1.237PhePhe: 1.237 ± 0.357
2.475PheGly: 2.475 ± 0.571
0.381PheHis: 0.381 ± 0.225
1.142PheIle: 1.142 ± 0.318
1.999PheLys: 1.999 ± 0.519
2.665PheLeu: 2.665 ± 0.772
0.857PheMet: 0.857 ± 0.278
3.046PheAsn: 3.046 ± 0.507
1.713PhePro: 1.713 ± 0.467
0.952PheGln: 0.952 ± 0.251
1.808PheArg: 1.808 ± 0.528
1.618PheSer: 1.618 ± 0.49
2.284PheThr: 2.284 ± 0.401
1.047PheVal: 1.047 ± 0.301
0.476PheTrp: 0.476 ± 0.213
1.142PheTyr: 1.142 ± 0.426
0.0PheXaa: 0.0 ± 0.0
Gly
7.9GlyAla: 7.9 ± 1.579
0.666GlyCys: 0.666 ± 0.332
2.856GlyAsp: 2.856 ± 0.655
4.474GlyGlu: 4.474 ± 0.712
2.665GlyPhe: 2.665 ± 0.739
8.471GlyGly: 8.471 ± 3.282
1.047GlyHis: 1.047 ± 0.357
3.617GlyIle: 3.617 ± 0.572
4.759GlyLys: 4.759 ± 0.633
6.472GlyLeu: 6.472 ± 0.799
1.904GlyMet: 1.904 ± 0.531
2.665GlyAsn: 2.665 ± 0.652
2.094GlyPro: 2.094 ± 0.382
2.57GlyGln: 2.57 ± 0.505
3.331GlyArg: 3.331 ± 0.673
5.14GlySer: 5.14 ± 1.083
6.472GlyThr: 6.472 ± 1.42
4.854GlyVal: 4.854 ± 0.513
2.284GlyTrp: 2.284 ± 0.539
2.856GlyTyr: 2.856 ± 0.546
0.0GlyXaa: 0.0 ± 0.0
His
1.523HisAla: 1.523 ± 0.419
0.095HisCys: 0.095 ± 0.09
0.666HisAsp: 0.666 ± 0.294
0.857HisGlu: 0.857 ± 0.279
0.286HisPhe: 0.286 ± 0.21
0.857HisGly: 0.857 ± 0.312
0.381HisHis: 0.381 ± 0.24
0.761HisIle: 0.761 ± 0.283
0.666HisLys: 0.666 ± 0.286
0.857HisLeu: 0.857 ± 0.362
0.19HisMet: 0.19 ± 0.148
0.761HisAsn: 0.761 ± 0.32
1.333HisPro: 1.333 ± 0.317
0.286HisGln: 0.286 ± 0.168
0.571HisArg: 0.571 ± 0.25
1.142HisSer: 1.142 ± 0.319
0.952HisThr: 0.952 ± 0.371
0.761HisVal: 0.761 ± 0.29
0.286HisTrp: 0.286 ± 0.185
0.571HisTyr: 0.571 ± 0.332
0.0HisXaa: 0.0 ± 0.0
Ile
6.187IleAla: 6.187 ± 0.813
0.571IleCys: 0.571 ± 0.218
4.283IleAsp: 4.283 ± 0.698
1.808IleGlu: 1.808 ± 0.457
0.952IlePhe: 0.952 ± 0.258
3.141IleGly: 3.141 ± 0.633
0.666IleHis: 0.666 ± 0.236
2.76IleIle: 2.76 ± 0.507
3.617IleLys: 3.617 ± 0.609
3.617IleLeu: 3.617 ± 0.616
1.047IleMet: 1.047 ± 0.415
2.76IleAsn: 2.76 ± 0.623
1.523IlePro: 1.523 ± 0.38
1.999IleGln: 1.999 ± 0.454
2.38IleArg: 2.38 ± 0.429
3.903IleSer: 3.903 ± 0.519
5.14IleThr: 5.14 ± 0.826
2.665IleVal: 2.665 ± 0.505
0.761IleTrp: 0.761 ± 0.366
2.094IleTyr: 2.094 ± 0.553
0.0IleXaa: 0.0 ± 0.0
Lys
5.711LysAla: 5.711 ± 0.954
0.476LysCys: 0.476 ± 0.265
2.665LysAsp: 2.665 ± 0.462
4.378LysGlu: 4.378 ± 0.931
1.808LysPhe: 1.808 ± 0.523
3.903LysGly: 3.903 ± 0.846
0.761LysHis: 0.761 ± 0.315
2.856LysIle: 2.856 ± 0.626
3.141LysLys: 3.141 ± 0.635
3.712LysLeu: 3.712 ± 0.602
1.713LysMet: 1.713 ± 0.538
2.951LysAsn: 2.951 ± 0.48
2.475LysPro: 2.475 ± 0.505
3.331LysGln: 3.331 ± 0.504
3.141LysArg: 3.141 ± 0.475
3.236LysSer: 3.236 ± 0.54
5.521LysThr: 5.521 ± 0.839
3.617LysVal: 3.617 ± 0.641
1.523LysTrp: 1.523 ± 0.427
1.904LysTyr: 1.904 ± 0.412
0.0LysXaa: 0.0 ± 0.0
Leu
7.805LeuAla: 7.805 ± 1.178
0.476LeuCys: 0.476 ± 0.219
5.711LeuAsp: 5.711 ± 0.744
4.569LeuGlu: 4.569 ± 0.874
1.808LeuPhe: 1.808 ± 0.407
4.95LeuGly: 4.95 ± 0.605
1.237LeuHis: 1.237 ± 0.325
4.569LeuIle: 4.569 ± 0.842
4.378LeuLys: 4.378 ± 0.75
5.711LeuLeu: 5.711 ± 0.653
1.999LeuMet: 1.999 ± 0.369
3.236LeuAsn: 3.236 ± 0.549
3.427LeuPro: 3.427 ± 0.489
1.999LeuGln: 1.999 ± 0.309
5.711LeuArg: 5.711 ± 0.803
6.568LeuSer: 6.568 ± 0.896
6.377LeuThr: 6.377 ± 0.938
3.522LeuVal: 3.522 ± 0.454
1.428LeuTrp: 1.428 ± 0.434
2.856LeuTyr: 2.856 ± 0.466
0.0LeuXaa: 0.0 ± 0.0
Met
3.522MetAla: 3.522 ± 0.521
0.381MetCys: 0.381 ± 0.221
1.333MetAsp: 1.333 ± 0.471
0.666MetGlu: 0.666 ± 0.222
0.666MetPhe: 0.666 ± 0.289
1.904MetGly: 1.904 ± 0.346
0.19MetHis: 0.19 ± 0.111
1.618MetIle: 1.618 ± 0.363
1.904MetLys: 1.904 ± 0.551
1.808MetLeu: 1.808 ± 0.428
0.476MetMet: 0.476 ± 0.241
0.381MetAsn: 0.381 ± 0.209
0.857MetPro: 0.857 ± 0.235
0.761MetGln: 0.761 ± 0.208
1.237MetArg: 1.237 ± 0.33
2.284MetSer: 2.284 ± 0.54
1.713MetThr: 1.713 ± 0.471
1.142MetVal: 1.142 ± 0.325
0.381MetTrp: 0.381 ± 0.171
0.571MetTyr: 0.571 ± 0.318
0.0MetXaa: 0.0 ± 0.0
Asn
4.759AsnAla: 4.759 ± 0.778
0.19AsnCys: 0.19 ± 0.149
3.617AsnAsp: 3.617 ± 0.552
2.475AsnGlu: 2.475 ± 0.536
1.713AsnPhe: 1.713 ± 0.369
4.664AsnGly: 4.664 ± 0.675
0.476AsnHis: 0.476 ± 0.307
2.665AsnIle: 2.665 ± 0.633
2.76AsnLys: 2.76 ± 0.385
3.427AsnLeu: 3.427 ± 0.704
0.857AsnMet: 0.857 ± 0.269
2.094AsnAsn: 2.094 ± 0.34
2.284AsnPro: 2.284 ± 0.503
2.284AsnGln: 2.284 ± 0.383
2.189AsnArg: 2.189 ± 0.443
3.236AsnSer: 3.236 ± 0.788
3.807AsnThr: 3.807 ± 0.7
1.904AsnVal: 1.904 ± 0.414
1.142AsnTrp: 1.142 ± 0.382
2.189AsnTyr: 2.189 ± 0.443
0.0AsnXaa: 0.0 ± 0.0
Pro
3.807ProAla: 3.807 ± 0.538
0.381ProCys: 0.381 ± 0.192
2.475ProAsp: 2.475 ± 0.542
3.236ProGlu: 3.236 ± 0.704
1.237ProPhe: 1.237 ± 0.28
2.57ProGly: 2.57 ± 0.613
0.476ProHis: 0.476 ± 0.188
1.904ProIle: 1.904 ± 0.431
3.236ProLys: 3.236 ± 0.632
2.094ProLeu: 2.094 ± 0.431
1.237ProMet: 1.237 ± 0.255
2.094ProAsn: 2.094 ± 0.549
1.904ProPro: 1.904 ± 0.551
1.428ProGln: 1.428 ± 0.399
1.618ProArg: 1.618 ± 0.433
3.236ProSer: 3.236 ± 0.711
3.522ProThr: 3.522 ± 0.836
2.57ProVal: 2.57 ± 0.491
0.666ProTrp: 0.666 ± 0.211
0.857ProTyr: 0.857 ± 0.32
0.0ProXaa: 0.0 ± 0.0
Gln
4.664GlnAla: 4.664 ± 0.702
0.0GlnCys: 0.0 ± 0.0
2.38GlnAsp: 2.38 ± 0.452
3.046GlnGlu: 3.046 ± 0.585
1.237GlnPhe: 1.237 ± 0.335
2.856GlnGly: 2.856 ± 0.385
0.571GlnHis: 0.571 ± 0.289
2.76GlnIle: 2.76 ± 0.55
2.665GlnLys: 2.665 ± 0.696
2.665GlnLeu: 2.665 ± 0.468
1.333GlnMet: 1.333 ± 0.37
2.57GlnAsn: 2.57 ± 0.37
1.428GlnPro: 1.428 ± 0.399
2.38GlnGln: 2.38 ± 0.503
1.808GlnArg: 1.808 ± 0.599
1.808GlnSer: 1.808 ± 0.397
3.427GlnThr: 3.427 ± 0.571
2.76GlnVal: 2.76 ± 0.424
0.476GlnTrp: 0.476 ± 0.198
1.428GlnTyr: 1.428 ± 0.406
0.0GlnXaa: 0.0 ± 0.0
Arg
4.664ArgAla: 4.664 ± 1.245
0.095ArgCys: 0.095 ± 0.098
2.57ArgAsp: 2.57 ± 0.579
3.141ArgGlu: 3.141 ± 0.696
1.523ArgPhe: 1.523 ± 0.349
2.38ArgGly: 2.38 ± 0.519
0.666ArgHis: 0.666 ± 0.261
2.284ArgIle: 2.284 ± 0.71
3.807ArgLys: 3.807 ± 0.594
4.283ArgLeu: 4.283 ± 0.605
1.142ArgMet: 1.142 ± 0.357
2.38ArgAsn: 2.38 ± 0.507
1.618ArgPro: 1.618 ± 0.348
2.856ArgGln: 2.856 ± 0.505
2.284ArgArg: 2.284 ± 0.752
2.951ArgSer: 2.951 ± 0.682
3.141ArgThr: 3.141 ± 0.648
2.951ArgVal: 2.951 ± 0.53
0.381ArgTrp: 0.381 ± 0.156
2.284ArgTyr: 2.284 ± 0.463
0.0ArgXaa: 0.0 ± 0.0
Ser
9.423SerAla: 9.423 ± 3.222
0.476SerCys: 0.476 ± 0.266
4.283SerAsp: 4.283 ± 0.54
2.951SerGlu: 2.951 ± 0.716
1.713SerPhe: 1.713 ± 0.415
6.948SerGly: 6.948 ± 1.311
1.142SerHis: 1.142 ± 0.351
2.57SerIle: 2.57 ± 0.466
2.76SerLys: 2.76 ± 0.43
5.14SerLeu: 5.14 ± 0.82
1.999SerMet: 1.999 ± 0.357
3.712SerAsn: 3.712 ± 0.557
2.951SerPro: 2.951 ± 0.521
3.046SerGln: 3.046 ± 0.525
2.284SerArg: 2.284 ± 0.638
4.474SerSer: 4.474 ± 1.295
6.568SerThr: 6.568 ± 1.213
3.998SerVal: 3.998 ± 0.572
0.476SerTrp: 0.476 ± 0.175
2.665SerTyr: 2.665 ± 0.46
0.0SerXaa: 0.0 ± 0.0
Thr
8.186ThrAla: 8.186 ± 1.579
0.381ThrCys: 0.381 ± 0.224
3.807ThrAsp: 3.807 ± 0.65
4.283ThrGlu: 4.283 ± 0.845
2.951ThrPhe: 2.951 ± 0.616
6.092ThrGly: 6.092 ± 1.202
1.047ThrHis: 1.047 ± 0.395
4.188ThrIle: 4.188 ± 0.57
3.046ThrLys: 3.046 ± 0.619
6.948ThrLeu: 6.948 ± 1.019
1.237ThrMet: 1.237 ± 0.286
3.046ThrAsn: 3.046 ± 0.561
5.14ThrPro: 5.14 ± 0.778
3.903ThrGln: 3.903 ± 0.605
2.475ThrArg: 2.475 ± 0.458
7.615ThrSer: 7.615 ± 2.596
5.045ThrThr: 5.045 ± 1.136
5.616ThrVal: 5.616 ± 0.856
0.952ThrTrp: 0.952 ± 0.223
2.38ThrTyr: 2.38 ± 0.486
0.0ThrXaa: 0.0 ± 0.0
Val
5.521ValAla: 5.521 ± 1.084
0.761ValCys: 0.761 ± 0.302
4.093ValAsp: 4.093 ± 0.592
3.141ValGlu: 3.141 ± 0.633
2.38ValPhe: 2.38 ± 0.573
3.712ValGly: 3.712 ± 0.637
0.761ValHis: 0.761 ± 0.257
2.189ValIle: 2.189 ± 0.433
3.522ValLys: 3.522 ± 0.662
4.474ValLeu: 4.474 ± 0.5
1.333ValMet: 1.333 ± 0.337
3.236ValAsn: 3.236 ± 0.464
2.38ValPro: 2.38 ± 0.393
2.189ValGln: 2.189 ± 0.488
2.951ValArg: 2.951 ± 0.917
3.998ValSer: 3.998 ± 0.42
4.95ValThr: 4.95 ± 1.17
4.664ValVal: 4.664 ± 0.603
1.047ValTrp: 1.047 ± 0.371
1.618ValTyr: 1.618 ± 0.427
0.0ValXaa: 0.0 ± 0.0
Trp
1.713TrpAla: 1.713 ± 0.482
0.381TrpCys: 0.381 ± 0.218
1.333TrpAsp: 1.333 ± 0.369
1.333TrpGlu: 1.333 ± 0.438
0.666TrpPhe: 0.666 ± 0.218
0.761TrpGly: 0.761 ± 0.301
0.095TrpHis: 0.095 ± 0.128
0.952TrpIle: 0.952 ± 0.307
0.381TrpLys: 0.381 ± 0.143
0.571TrpLeu: 0.571 ± 0.215
0.286TrpMet: 0.286 ± 0.14
0.761TrpAsn: 0.761 ± 0.247
0.0TrpPro: 0.0 ± 0.0
0.761TrpGln: 0.761 ± 0.283
0.857TrpArg: 0.857 ± 0.234
1.047TrpSer: 1.047 ± 0.28
1.142TrpThr: 1.142 ± 0.282
1.047TrpVal: 1.047 ± 0.35
0.381TrpTrp: 0.381 ± 0.202
0.952TrpTyr: 0.952 ± 0.259
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.57TyrAla: 2.57 ± 0.548
0.095TyrCys: 0.095 ± 0.124
1.808TyrAsp: 1.808 ± 0.403
2.094TyrGlu: 2.094 ± 0.404
0.857TyrPhe: 0.857 ± 0.254
3.712TyrGly: 3.712 ± 0.693
0.286TyrHis: 0.286 ± 0.166
1.904TyrIle: 1.904 ± 0.508
1.904TyrLys: 1.904 ± 0.472
3.522TyrLeu: 3.522 ± 0.561
0.571TyrMet: 0.571 ± 0.236
2.189TyrAsn: 2.189 ± 0.398
1.237TyrPro: 1.237 ± 0.293
1.808TyrGln: 1.808 ± 0.435
1.333TyrArg: 1.333 ± 0.383
2.665TyrSer: 2.665 ± 0.661
2.475TyrThr: 2.475 ± 0.425
1.999TyrVal: 1.999 ± 0.552
0.761TyrTrp: 0.761 ± 0.276
1.142TyrTyr: 1.142 ± 0.312
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (10507 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski