Amino acid dipepetide frequency for bacterium HR18

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.253AlaAla: 12.253 ± 0.172	1.005AlaCys: 1.005 ± 0.039	4.577AlaAsp: 4.577 ± 0.066	6.963AlaGlu: 6.963 ± 0.109	4.445AlaPhe: 4.445 ± 0.075	7.289AlaGly: 7.289 ± 0.095	2.52AlaHis: 2.52 ± 0.053	4.742AlaIle: 4.742 ± 0.084	1.924AlaLys: 1.924 ± 0.057	14.588AlaLeu: 14.588 ± 0.176	2.155AlaMet: 2.155 ± 0.051	2.109AlaAsn: 2.109 ± 0.049	4.663AlaPro: 4.663 ± 0.076	5.712AlaGln: 5.712 ± 0.09	8.784AlaArg: 8.784 ± 0.118	4.616AlaSer: 4.616 ± 0.073	5.358AlaThr: 5.358 ± 0.086	7.499AlaVal: 7.499 ± 0.101	1.815AlaTrp: 1.815 ± 0.058	3.659AlaTyr: 3.659 ± 0.064	0.0AlaXaa: 0.0 ± 0.0
Cys	0.738CysAla: 0.738 ± 0.034	0.115CysCys: 0.115 ± 0.012	0.366CysAsp: 0.366 ± 0.023	0.342CysGlu: 0.342 ± 0.02	0.284CysPhe: 0.284 ± 0.018	0.724CysGly: 0.724 ± 0.032	0.243CysHis: 0.243 ± 0.019	0.365CysIle: 0.365 ± 0.022	0.163CysLys: 0.163 ± 0.013	0.795CysLeu: 0.795 ± 0.032	0.12CysMet: 0.12 ± 0.011	0.202CysAsn: 0.202 ± 0.015	0.488CysPro: 0.488 ± 0.024	0.293CysGln: 0.293 ± 0.018	0.585CysArg: 0.585 ± 0.026	0.382CysSer: 0.382 ± 0.023	0.444CysThr: 0.444 ± 0.025	0.489CysVal: 0.489 ± 0.023	0.133CysTrp: 0.133 ± 0.011	0.293CysTyr: 0.293 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	4.544AspAla: 4.544 ± 0.067	0.244AspCys: 0.244 ± 0.019	1.726AspAsp: 1.726 ± 0.049	2.883AspGlu: 2.883 ± 0.07	1.972AspPhe: 1.972 ± 0.049	3.702AspGly: 3.702 ± 0.083	0.957AspHis: 0.957 ± 0.037	1.776AspIle: 1.776 ± 0.05	0.87AspLys: 0.87 ± 0.039	5.275AspLeu: 5.275 ± 0.085	0.6AspMet: 0.6 ± 0.026	0.924AspAsn: 0.924 ± 0.036	3.801AspPro: 3.801 ± 0.074	1.501AspGln: 1.501 ± 0.039	3.844AspArg: 3.844 ± 0.075	1.871AspSer: 1.871 ± 0.055	2.538AspThr: 2.538 ± 0.067	3.791AspVal: 3.791 ± 0.07	0.878AspTrp: 0.878 ± 0.032	1.728AspTyr: 1.728 ± 0.048	0.0AspXaa: 0.0 ± 0.0
Glu	10.162GluAla: 10.162 ± 0.164	0.28GluCys: 0.28 ± 0.018	2.718GluAsp: 2.718 ± 0.064	4.459GluGlu: 4.459 ± 0.104	1.452GluPhe: 1.452 ± 0.042	5.237GluGly: 5.237 ± 0.092	1.497GluHis: 1.497 ± 0.046	2.996GluIle: 2.996 ± 0.069	1.911GluLys: 1.911 ± 0.056	6.703GluLeu: 6.703 ± 0.095	1.194GluMet: 1.194 ± 0.039	1.641GluAsn: 1.641 ± 0.048	2.965GluPro: 2.965 ± 0.055	3.084GluGln: 3.084 ± 0.06	5.715GluArg: 5.715 ± 0.107	2.031GluSer: 2.031 ± 0.049	3.632GluThr: 3.632 ± 0.077	5.425GluVal: 5.425 ± 0.08	0.655GluTrp: 0.655 ± 0.03	1.241GluTyr: 1.241 ± 0.043	0.0GluXaa: 0.0 ± 0.0
Phe	3.603PheAla: 3.603 ± 0.075	0.34PheCys: 0.34 ± 0.02	2.241PheAsp: 2.241 ± 0.054	2.611PheGlu: 2.611 ± 0.058	1.808PhePhe: 1.808 ± 0.054	3.048PheGly: 3.048 ± 0.061	0.741PheHis: 0.741 ± 0.029	1.596PheIle: 1.596 ± 0.045	0.782PheLys: 0.782 ± 0.032	3.753PheLeu: 3.753 ± 0.073	0.681PheMet: 0.681 ± 0.028	1.086PheAsn: 1.086 ± 0.043	1.883PhePro: 1.883 ± 0.046	1.397PheGln: 1.397 ± 0.037	2.922PheArg: 2.922 ± 0.065	2.156PheSer: 2.156 ± 0.059	2.182PheThr: 2.182 ± 0.049	2.915PheVal: 2.915 ± 0.056	0.803PheTrp: 0.803 ± 0.032	1.444PheTyr: 1.444 ± 0.047	0.0PheXaa: 0.0 ± 0.0
Gly	6.552GlyAla: 6.552 ± 0.098	0.795GlyCys: 0.795 ± 0.035	3.266GlyAsp: 3.266 ± 0.065	3.804GlyGlu: 3.804 ± 0.064	3.184GlyPhe: 3.184 ± 0.07	5.506GlyGly: 5.506 ± 0.109	1.684GlyHis: 1.684 ± 0.046	4.086GlyIle: 4.086 ± 0.081	2.207GlyLys: 2.207 ± 0.056	9.415GlyLeu: 9.415 ± 0.138	1.712GlyMet: 1.712 ± 0.048	1.893GlyAsn: 1.893 ± 0.047	3.234GlyPro: 3.234 ± 0.063	3.457GlyGln: 3.457 ± 0.062	6.274GlyArg: 6.274 ± 0.09	3.583GlySer: 3.583 ± 0.073	4.337GlyThr: 4.337 ± 0.083	5.63GlyVal: 5.63 ± 0.095	1.528GlyTrp: 1.528 ± 0.049	2.927GlyTyr: 2.927 ± 0.058	0.0GlyXaa: 0.0 ± 0.0
His	2.207HisAla: 2.207 ± 0.057	0.212HisCys: 0.212 ± 0.018	0.896HisAsp: 0.896 ± 0.031	1.206HisGlu: 1.206 ± 0.039	1.093HisPhe: 1.093 ± 0.035	1.662HisGly: 1.662 ± 0.05	0.699HisHis: 0.699 ± 0.029	1.058HisIle: 1.058 ± 0.041	0.45HisLys: 0.45 ± 0.023	2.938HisLeu: 2.938 ± 0.064	0.393HisMet: 0.393 ± 0.02	0.579HisAsn: 0.579 ± 0.028	1.984HisPro: 1.984 ± 0.054	0.988HisGln: 0.988 ± 0.038	1.947HisArg: 1.947 ± 0.05	0.785HisSer: 0.785 ± 0.031	1.24HisThr: 1.24 ± 0.038	1.956HisVal: 1.956 ± 0.05	0.47HisTrp: 0.47 ± 0.026	0.979HisTyr: 0.979 ± 0.036	0.0HisXaa: 0.0 ± 0.0
Ile	4.896IleAla: 4.896 ± 0.087	0.371IleCys: 0.371 ± 0.021	2.904IleAsp: 2.904 ± 0.06	3.524IleGlu: 3.524 ± 0.075	1.61IlePhe: 1.61 ± 0.05	4.116IleGly: 4.116 ± 0.083	0.922IleHis: 0.922 ± 0.033	1.759IleIle: 1.759 ± 0.054	0.969IleLys: 0.969 ± 0.04	4.234IleLeu: 4.234 ± 0.079	0.591IleMet: 0.591 ± 0.027	1.196IleAsn: 1.196 ± 0.04	2.746IlePro: 2.746 ± 0.058	1.548IleGln: 1.548 ± 0.043	3.399IleArg: 3.399 ± 0.071	1.982IleSer: 1.982 ± 0.053	2.539IleThr: 2.539 ± 0.057	3.544IleVal: 3.544 ± 0.059	0.574IleTrp: 0.574 ± 0.031	1.384IleTyr: 1.384 ± 0.04	0.0IleXaa: 0.0 ± 0.0
Lys	2.945LysAla: 2.945 ± 0.06	0.11LysCys: 0.11 ± 0.012	0.991LysAsp: 0.991 ± 0.036	1.423LysGlu: 1.423 ± 0.051	0.566LysPhe: 0.566 ± 0.025	1.798LysGly: 1.798 ± 0.051	0.537LysHis: 0.537 ± 0.026	1.119LysIle: 1.119 ± 0.039	0.813LysLys: 0.813 ± 0.036	2.606LysLeu: 2.606 ± 0.058	0.468LysMet: 0.468 ± 0.022	0.678LysAsn: 0.678 ± 0.034	1.531LysPro: 1.531 ± 0.039	1.045LysGln: 1.045 ± 0.033	1.851LysArg: 1.851 ± 0.048	0.965LysSer: 0.965 ± 0.039	1.421LysThr: 1.421 ± 0.049	1.646LysVal: 1.646 ± 0.052	0.225LysTrp: 0.225 ± 0.017	0.617LysTyr: 0.617 ± 0.029	0.0LysXaa: 0.0 ± 0.0
Leu	12.819LeuAla: 12.819 ± 0.157	0.908LeuCys: 0.908 ± 0.031	5.292LeuAsp: 5.292 ± 0.083	8.937LeuGlu: 8.937 ± 0.122	4.146LeuPhe: 4.146 ± 0.086	8.927LeuGly: 8.927 ± 0.116	2.978LeuHis: 2.978 ± 0.065	5.08LeuIle: 5.08 ± 0.083	3.113LeuLys: 3.113 ± 0.069	14.309LeuLeu: 14.309 ± 0.209	2.242LeuMet: 2.242 ± 0.048	2.825LeuAsn: 2.825 ± 0.062	7.034LeuPro: 7.034 ± 0.102	5.744LeuGln: 5.744 ± 0.085	10.317LeuArg: 10.317 ± 0.126	6.159LeuSer: 6.159 ± 0.097	6.164LeuThr: 6.164 ± 0.101	8.383LeuVal: 8.383 ± 0.123	1.984LeuTrp: 1.984 ± 0.065	3.509LeuTyr: 3.509 ± 0.067	0.0LeuXaa: 0.0 ± 0.0
Met	2.23MetAla: 2.23 ± 0.055	0.08MetCys: 0.08 ± 0.01	0.793MetAsp: 0.793 ± 0.034	1.087MetGlu: 1.087 ± 0.037	0.413MetPhe: 0.413 ± 0.021	1.483MetGly: 1.483 ± 0.042	0.544MetHis: 0.544 ± 0.029	0.82MetIle: 0.82 ± 0.029	0.594MetLys: 0.594 ± 0.026	2.258MetLeu: 2.258 ± 0.053	0.4MetMet: 0.4 ± 0.021	0.509MetAsn: 0.509 ± 0.025	1.336MetPro: 1.336 ± 0.044	0.897MetGln: 0.897 ± 0.032	1.639MetArg: 1.639 ± 0.045	0.795MetSer: 0.795 ± 0.029	0.882MetThr: 0.882 ± 0.034	1.272MetVal: 1.272 ± 0.043	0.174MetTrp: 0.174 ± 0.015	0.334MetTyr: 0.334 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	2.629AsnAla: 2.629 ± 0.066	0.188AsnCys: 0.188 ± 0.015	1.092AsnAsp: 1.092 ± 0.039	1.227AsnGlu: 1.227 ± 0.039	0.907AsnPhe: 0.907 ± 0.037	1.979AsnGly: 1.979 ± 0.058	0.505AsnHis: 0.505 ± 0.024	1.005AsnIle: 1.005 ± 0.039	0.43AsnLys: 0.43 ± 0.021	2.848AsnLeu: 2.848 ± 0.069	0.385AsnMet: 0.385 ± 0.022	0.741AsnAsn: 0.741 ± 0.036	2.133AsnPro: 2.133 ± 0.054	0.927AsnGln: 0.927 ± 0.036	1.924AsnArg: 1.924 ± 0.053	0.844AsnSer: 0.844 ± 0.034	1.252AsnThr: 1.252 ± 0.039	1.987AsnVal: 1.987 ± 0.064	0.418AsnTrp: 0.418 ± 0.024	0.822AsnTyr: 0.822 ± 0.036	0.0AsnXaa: 0.0 ± 0.0
Pro	5.403ProAla: 5.403 ± 0.088	0.333ProCys: 0.333 ± 0.021	3.345ProAsp: 3.345 ± 0.077	5.738ProGlu: 5.738 ± 0.088	2.309ProPhe: 2.309 ± 0.057	4.261ProGly: 4.261 ± 0.07	1.271ProHis: 1.271 ± 0.04	2.505ProIle: 2.505 ± 0.052	1.371ProLys: 1.371 ± 0.039	6.18ProLeu: 6.18 ± 0.096	1.124ProMet: 1.124 ± 0.036	1.619ProAsn: 1.619 ± 0.049	3.403ProPro: 3.403 ± 0.08	2.189ProGln: 2.189 ± 0.05	3.328ProArg: 3.328 ± 0.063	2.93ProSer: 2.93 ± 0.053	2.876ProThr: 2.876 ± 0.062	4.578ProVal: 4.578 ± 0.082	0.891ProTrp: 0.891 ± 0.032	1.854ProTyr: 1.854 ± 0.048	0.0ProXaa: 0.0 ± 0.0
Gln	6.198GlnAla: 6.198 ± 0.087	0.249GlnCys: 0.249 ± 0.016	1.584GlnAsp: 1.584 ± 0.043	3.006GlnGlu: 3.006 ± 0.074	1.276GlnPhe: 1.276 ± 0.04	3.216GlnGly: 3.216 ± 0.06	1.227GlnHis: 1.227 ± 0.038	1.859GlnIle: 1.859 ± 0.044	1.156GlnLys: 1.156 ± 0.036	5.54GlnLeu: 5.54 ± 0.093	0.791GlnMet: 0.791 ± 0.029	0.951GlnAsn: 0.951 ± 0.036	2.692GlnPro: 2.692 ± 0.064	2.888GlnGln: 2.888 ± 0.08	3.955GlnArg: 3.955 ± 0.07	1.534GlnSer: 1.534 ± 0.042	2.441GlnThr: 2.441 ± 0.058	3.408GlnVal: 3.408 ± 0.064	0.631GlnTrp: 0.631 ± 0.028	1.048GlnTyr: 1.048 ± 0.041	0.0GlnXaa: 0.0 ± 0.0
Arg	7.192ArgAla: 7.192 ± 0.095	0.589ArgCys: 0.589 ± 0.03	3.209ArgAsp: 3.209 ± 0.053	4.713ArgGlu: 4.713 ± 0.085	3.654ArgPhe: 3.654 ± 0.069	4.431ArgGly: 4.431 ± 0.072	1.98ArgHis: 1.98 ± 0.051	4.193ArgIle: 4.193 ± 0.08	2.174ArgLys: 2.174 ± 0.058	11.246ArgLeu: 11.246 ± 0.137	1.759ArgMet: 1.759 ± 0.046	1.914ArgAsn: 1.914 ± 0.057	4.535ArgPro: 4.535 ± 0.068	4.553ArgGln: 4.553 ± 0.078	7.078ArgArg: 7.078 ± 0.095	3.311ArgSer: 3.311 ± 0.06	4.077ArgThr: 4.077 ± 0.068	5.552ArgVal: 5.552 ± 0.076	1.736ArgTrp: 1.736 ± 0.05	3.547ArgTyr: 3.547 ± 0.072	0.0ArgXaa: 0.0 ± 0.0
Ser	4.201SerAla: 4.201 ± 0.077	0.38SerCys: 0.38 ± 0.022	2.004SerAsp: 2.004 ± 0.047	2.461SerGlu: 2.461 ± 0.051	1.772SerPhe: 1.772 ± 0.044	4.111SerGly: 4.111 ± 0.067	0.855SerHis: 0.855 ± 0.037	2.033SerIle: 2.033 ± 0.056	0.979SerLys: 0.979 ± 0.036	5.216SerLeu: 5.216 ± 0.087	0.915SerMet: 0.915 ± 0.032	1.086SerAsn: 1.086 ± 0.037	2.683SerPro: 2.683 ± 0.06	1.576SerGln: 1.576 ± 0.039	3.2SerArg: 3.2 ± 0.061	2.277SerSer: 2.277 ± 0.057	2.44SerThr: 2.44 ± 0.06	3.279SerVal: 3.279 ± 0.068	0.887SerTrp: 0.887 ± 0.036	1.406SerTyr: 1.406 ± 0.054	0.0SerXaa: 0.0 ± 0.0
Thr	5.297ThrAla: 5.297 ± 0.093	0.343ThrCys: 0.343 ± 0.019	2.378ThrAsp: 2.378 ± 0.057	2.848ThrGlu: 2.848 ± 0.058	2.42ThrPhe: 2.42 ± 0.059	4.488ThrGly: 4.488 ± 0.074	1.289ThrHis: 1.289 ± 0.039	2.531ThrIle: 2.531 ± 0.053	0.952ThrLys: 0.952 ± 0.034	7.449ThrLeu: 7.449 ± 0.117	0.793ThrMet: 0.793 ± 0.029	1.217ThrAsn: 1.217 ± 0.043	3.723ThrPro: 3.723 ± 0.081	1.976ThrGln: 1.976 ± 0.052	3.512ThrArg: 3.512 ± 0.065	2.342ThrSer: 2.342 ± 0.049	2.99ThrThr: 2.99 ± 0.065	4.026ThrVal: 4.026 ± 0.072	0.738ThrTrp: 0.738 ± 0.031	1.899ThrTyr: 1.899 ± 0.046	0.0ThrXaa: 0.0 ± 0.0
Val	8.104ValAla: 8.104 ± 0.114	0.619ValCys: 0.619 ± 0.032	3.644ValAsp: 3.644 ± 0.064	4.699ValGlu: 4.699 ± 0.079	2.762ValPhe: 2.762 ± 0.054	5.266ValGly: 5.266 ± 0.086	1.989ValHis: 1.989 ± 0.057	3.235ValIle: 3.235 ± 0.062	1.393ValLys: 1.393 ± 0.04	9.694ValLeu: 9.694 ± 0.122	1.283ValMet: 1.283 ± 0.047	1.659ValAsn: 1.659 ± 0.046	4.061ValPro: 4.061 ± 0.07	3.431ValGln: 3.431 ± 0.068	6.61ValArg: 6.61 ± 0.086	3.267ValSer: 3.267 ± 0.064	3.827ValThr: 3.827 ± 0.07	6.813ValVal: 6.813 ± 0.12	1.171ValTrp: 1.171 ± 0.038	2.362ValTyr: 2.362 ± 0.053	0.0ValXaa: 0.0 ± 0.0
Trp	1.536TrpAla: 1.536 ± 0.042	0.137TrpCys: 0.137 ± 0.013	0.687TrpAsp: 0.687 ± 0.027	0.915TrpGlu: 0.915 ± 0.033	0.574TrpPhe: 0.574 ± 0.026	1.141TrpGly: 1.141 ± 0.041	0.489TrpHis: 0.489 ± 0.025	0.882TrpIle: 0.882 ± 0.033	0.456TrpLys: 0.456 ± 0.023	2.147TrpLeu: 2.147 ± 0.055	0.477TrpMet: 0.477 ± 0.026	0.48TrpAsn: 0.48 ± 0.025	0.86TrpPro: 0.86 ± 0.038	0.986TrpGln: 0.986 ± 0.039	1.341TrpArg: 1.341 ± 0.043	0.774TrpSer: 0.774 ± 0.032	0.81TrpThr: 0.81 ± 0.033	1.104TrpVal: 1.104 ± 0.04	0.319TrpTrp: 0.319 ± 0.022	0.465TrpTyr: 0.465 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.17TyrAla: 3.17 ± 0.069	0.265TyrCys: 0.265 ± 0.02	1.712TyrAsp: 1.712 ± 0.044	2.151TyrGlu: 2.151 ± 0.052	1.345TyrPhe: 1.345 ± 0.041	2.69TyrGly: 2.69 ± 0.066	0.819TyrHis: 0.819 ± 0.031	1.165TyrIle: 1.165 ± 0.039	0.645TyrLys: 0.645 ± 0.026	3.675TyrLeu: 3.675 ± 0.073	0.459TyrMet: 0.459 ± 0.022	0.933TyrAsn: 0.933 ± 0.04	1.754TyrPro: 1.754 ± 0.051	1.457TyrGln: 1.457 ± 0.039	3.219TyrArg: 3.219 ± 0.069	1.159TyrSer: 1.159 ± 0.039	1.759TyrThr: 1.759 ± 0.052	2.592TyrVal: 2.592 ± 0.059	0.549TyrTrp: 0.549 ± 0.028	1.154TyrTyr: 1.154 ± 0.041	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2437 proteins (885271 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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