Amino acid dipepetide frequency for Arthrobacter phage Sporto

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.207AlaAla: 9.207 ± 1.004
0.35AlaCys: 0.35 ± 0.143
5.303AlaAsp: 5.303 ± 0.545
5.769AlaGlu: 5.769 ± 0.516
3.205AlaPhe: 3.205 ± 0.434
6.585AlaGly: 6.585 ± 1.551
1.923AlaHis: 1.923 ± 0.453
5.594AlaIle: 5.594 ± 0.601
5.361AlaLys: 5.361 ± 0.882
7.11AlaLeu: 7.11 ± 1.321
3.147AlaMet: 3.147 ± 0.714
2.564AlaAsn: 2.564 ± 0.442
3.322AlaPro: 3.322 ± 0.524
3.205AlaGln: 3.205 ± 0.424
4.72AlaArg: 4.72 ± 0.476
5.186AlaSer: 5.186 ± 0.585
5.186AlaThr: 5.186 ± 0.679
7.517AlaVal: 7.517 ± 0.606
1.515AlaTrp: 1.515 ± 0.469
2.564AlaTyr: 2.564 ± 0.371
0.0AlaXaa: 0.0 ± 0.0
Cys
0.466CysAla: 0.466 ± 0.18
0.0CysCys: 0.0 ± 0.0
0.466CysAsp: 0.466 ± 0.186
0.408CysGlu: 0.408 ± 0.147
0.233CysPhe: 0.233 ± 0.111
0.408CysGly: 0.408 ± 0.142
0.233CysHis: 0.233 ± 0.105
0.641CysIle: 0.641 ± 0.199
0.233CysLys: 0.233 ± 0.114
0.524CysLeu: 0.524 ± 0.178
0.117CysMet: 0.117 ± 0.074
0.117CysAsn: 0.117 ± 0.074
0.35CysPro: 0.35 ± 0.174
0.233CysGln: 0.233 ± 0.143
0.408CysArg: 0.408 ± 0.157
0.291CysSer: 0.291 ± 0.139
0.466CysThr: 0.466 ± 0.166
0.466CysVal: 0.466 ± 0.186
0.117CysTrp: 0.117 ± 0.086
0.175CysTyr: 0.175 ± 0.096
0.0CysXaa: 0.0 ± 0.0
Asp
6.469AspAla: 6.469 ± 0.615
0.816AspCys: 0.816 ± 0.29
3.555AspAsp: 3.555 ± 0.651
4.312AspGlu: 4.312 ± 0.577
2.972AspPhe: 2.972 ± 0.53
4.371AspGly: 4.371 ± 0.416
0.758AspHis: 0.758 ± 0.182
4.429AspIle: 4.429 ± 0.475
3.38AspLys: 3.38 ± 0.606
4.604AspLeu: 4.604 ± 0.505
1.632AspMet: 1.632 ± 0.3
2.681AspAsn: 2.681 ± 0.368
2.681AspPro: 2.681 ± 0.515
2.331AspGln: 2.331 ± 0.46
3.03AspArg: 3.03 ± 0.62
2.506AspSer: 2.506 ± 0.41
3.263AspThr: 3.263 ± 0.387
4.254AspVal: 4.254 ± 0.639
1.049AspTrp: 1.049 ± 0.226
1.923AspTyr: 1.923 ± 0.379
0.0AspXaa: 0.0 ± 0.0
Glu
4.953GluAla: 4.953 ± 0.448
0.408GluCys: 0.408 ± 0.183
3.38GluAsp: 3.38 ± 0.462
5.012GluGlu: 5.012 ± 0.717
2.273GluPhe: 2.273 ± 0.374
4.895GluGly: 4.895 ± 0.565
0.874GluHis: 0.874 ± 0.282
4.021GluIle: 4.021 ± 0.46
3.963GluLys: 3.963 ± 0.657
5.828GluLeu: 5.828 ± 0.58
2.04GluMet: 2.04 ± 0.275
1.748GluAsn: 1.748 ± 0.357
2.622GluPro: 2.622 ± 0.472
2.564GluGln: 2.564 ± 0.382
3.846GluArg: 3.846 ± 0.444
2.681GluSer: 2.681 ± 0.362
3.613GluThr: 3.613 ± 0.561
4.429GluVal: 4.429 ± 0.733
1.69GluTrp: 1.69 ± 0.368
2.448GluTyr: 2.448 ± 0.416
0.0GluXaa: 0.0 ± 0.0
Phe
2.448PheAla: 2.448 ± 0.4
0.291PheCys: 0.291 ± 0.125
2.564PheAsp: 2.564 ± 0.303
2.214PheGlu: 2.214 ± 0.327
1.515PhePhe: 1.515 ± 0.275
3.147PheGly: 3.147 ± 0.469
0.699PheHis: 0.699 ± 0.193
2.389PheIle: 2.389 ± 0.441
1.981PheLys: 1.981 ± 0.358
3.089PheLeu: 3.089 ± 0.355
1.166PheMet: 1.166 ± 0.259
1.981PheAsn: 1.981 ± 0.332
1.457PhePro: 1.457 ± 0.291
0.932PheGln: 0.932 ± 0.178
1.807PheArg: 1.807 ± 0.362
1.865PheSer: 1.865 ± 0.399
3.03PheThr: 3.03 ± 0.467
2.448PheVal: 2.448 ± 0.375
0.699PheTrp: 0.699 ± 0.204
1.515PheTyr: 1.515 ± 0.33
0.0PheXaa: 0.0 ± 0.0
Gly
7.517GlyAla: 7.517 ± 0.995
0.117GlyCys: 0.117 ± 0.079
4.779GlyAsp: 4.779 ± 0.534
3.963GlyGlu: 3.963 ± 0.428
3.147GlyPhe: 3.147 ± 0.41
6.235GlyGly: 6.235 ± 0.797
1.573GlyHis: 1.573 ± 0.354
4.545GlyIle: 4.545 ± 0.877
4.021GlyLys: 4.021 ± 0.502
6.527GlyLeu: 6.527 ± 1.121
2.972GlyMet: 2.972 ± 0.761
3.497GlyAsn: 3.497 ± 0.648
1.923GlyPro: 1.923 ± 0.357
2.273GlyGln: 2.273 ± 0.381
2.797GlyArg: 2.797 ± 0.365
5.653GlySer: 5.653 ± 0.669
5.653GlyThr: 5.653 ± 0.652
6.527GlyVal: 6.527 ± 0.806
1.69GlyTrp: 1.69 ± 0.333
2.739GlyTyr: 2.739 ± 0.521
0.0GlyXaa: 0.0 ± 0.0
His
1.865HisAla: 1.865 ± 0.469
0.0HisCys: 0.0 ± 0.0
0.758HisAsp: 0.758 ± 0.213
1.34HisGlu: 1.34 ± 0.299
0.408HisPhe: 0.408 ± 0.131
1.69HisGly: 1.69 ± 0.376
0.233HisHis: 0.233 ± 0.127
0.991HisIle: 0.991 ± 0.189
0.991HisLys: 0.991 ± 0.251
1.282HisLeu: 1.282 ± 0.261
0.524HisMet: 0.524 ± 0.181
0.816HisAsn: 0.816 ± 0.213
0.699HisPro: 0.699 ± 0.254
0.932HisGln: 0.932 ± 0.218
1.107HisArg: 1.107 ± 0.309
0.932HisSer: 0.932 ± 0.279
0.758HisThr: 0.758 ± 0.216
1.107HisVal: 1.107 ± 0.255
0.233HisTrp: 0.233 ± 0.119
1.457HisTyr: 1.457 ± 0.37
0.0HisXaa: 0.0 ± 0.0
Ile
5.536IleAla: 5.536 ± 0.804
0.466IleCys: 0.466 ± 0.168
4.662IleAsp: 4.662 ± 0.48
3.263IleGlu: 3.263 ± 0.382
1.981IlePhe: 1.981 ± 0.314
3.438IleGly: 3.438 ± 0.835
0.991IleHis: 0.991 ± 0.241
3.438IleIle: 3.438 ± 0.413
3.671IleLys: 3.671 ± 0.46
4.371IleLeu: 4.371 ± 0.625
1.69IleMet: 1.69 ± 0.376
2.972IleAsn: 2.972 ± 0.378
2.448IlePro: 2.448 ± 0.468
2.04IleGln: 2.04 ± 0.353
2.855IleArg: 2.855 ± 0.478
4.487IleSer: 4.487 ± 0.443
3.788IleThr: 3.788 ± 0.557
5.012IleVal: 5.012 ± 0.582
0.816IleTrp: 0.816 ± 0.225
1.807IleTyr: 1.807 ± 0.288
0.0IleXaa: 0.0 ± 0.0
Lys
6.177LysAla: 6.177 ± 0.656
0.117LysCys: 0.117 ± 0.079
3.322LysAsp: 3.322 ± 0.545
4.079LysGlu: 4.079 ± 0.56
2.564LysPhe: 2.564 ± 0.456
5.07LysGly: 5.07 ± 0.801
1.807LysHis: 1.807 ± 0.361
3.03LysIle: 3.03 ± 0.416
4.779LysLys: 4.779 ± 0.624
5.711LysLeu: 5.711 ± 0.652
2.389LysMet: 2.389 ± 0.378
2.564LysAsn: 2.564 ± 0.314
2.739LysPro: 2.739 ± 0.374
2.331LysGln: 2.331 ± 0.423
3.205LysArg: 3.205 ± 0.471
4.138LysSer: 4.138 ± 0.48
4.079LysThr: 4.079 ± 0.553
3.904LysVal: 3.904 ± 0.356
0.758LysTrp: 0.758 ± 0.213
1.69LysTyr: 1.69 ± 0.345
0.0LysXaa: 0.0 ± 0.0
Leu
7.867LeuAla: 7.867 ± 1.069
0.758LeuCys: 0.758 ± 0.271
5.012LeuAsp: 5.012 ± 0.582
4.662LeuGlu: 4.662 ± 0.445
2.564LeuPhe: 2.564 ± 0.365
6.76LeuGly: 6.76 ± 1.227
1.34LeuHis: 1.34 ± 0.282
5.128LeuIle: 5.128 ± 0.686
5.361LeuLys: 5.361 ± 0.739
4.604LeuLeu: 4.604 ± 0.582
2.448LeuMet: 2.448 ± 0.38
3.671LeuAsn: 3.671 ± 0.478
2.797LeuPro: 2.797 ± 0.384
2.448LeuGln: 2.448 ± 0.288
3.555LeuArg: 3.555 ± 0.569
4.953LeuSer: 4.953 ± 0.533
5.536LeuThr: 5.536 ± 0.653
5.653LeuVal: 5.653 ± 0.594
0.699LeuTrp: 0.699 ± 0.183
2.797LeuTyr: 2.797 ± 0.516
0.0LeuXaa: 0.0 ± 0.0
Met
3.03MetAla: 3.03 ± 0.609
0.233MetCys: 0.233 ± 0.11
1.399MetAsp: 1.399 ± 0.251
2.214MetGlu: 2.214 ± 0.503
1.107MetPhe: 1.107 ± 0.224
2.564MetGly: 2.564 ± 0.56
0.291MetHis: 0.291 ± 0.138
1.457MetIle: 1.457 ± 0.321
1.632MetLys: 1.632 ± 0.355
2.098MetLeu: 2.098 ± 0.312
0.816MetMet: 0.816 ± 0.285
1.166MetAsn: 1.166 ± 0.244
0.991MetPro: 0.991 ± 0.188
1.166MetGln: 1.166 ± 0.345
1.748MetArg: 1.748 ± 0.342
2.681MetSer: 2.681 ± 0.541
2.273MetThr: 2.273 ± 0.476
1.515MetVal: 1.515 ± 0.303
0.291MetTrp: 0.291 ± 0.172
0.932MetTyr: 0.932 ± 0.222
0.0MetXaa: 0.0 ± 0.0
Asn
2.797AsnAla: 2.797 ± 0.449
0.524AsnCys: 0.524 ± 0.177
2.331AsnAsp: 2.331 ± 0.336
2.972AsnGlu: 2.972 ± 0.461
2.04AsnPhe: 2.04 ± 0.396
4.371AsnGly: 4.371 ± 0.749
0.699AsnHis: 0.699 ± 0.19
2.564AsnIle: 2.564 ± 0.405
2.797AsnLys: 2.797 ± 0.495
3.497AsnLeu: 3.497 ± 0.481
1.457AsnMet: 1.457 ± 0.341
2.04AsnAsn: 2.04 ± 0.32
3.147AsnPro: 3.147 ± 0.469
1.282AsnGln: 1.282 ± 0.268
2.156AsnArg: 2.156 ± 0.418
2.331AsnSer: 2.331 ± 0.295
2.681AsnThr: 2.681 ± 0.367
2.622AsnVal: 2.622 ± 0.355
0.524AsnTrp: 0.524 ± 0.189
1.573AsnTyr: 1.573 ± 0.277
0.0AsnXaa: 0.0 ± 0.0
Pro
3.147ProAla: 3.147 ± 0.478
0.524ProCys: 0.524 ± 0.191
2.506ProAsp: 2.506 ± 0.45
3.904ProGlu: 3.904 ± 0.587
1.107ProPhe: 1.107 ± 0.228
2.797ProGly: 2.797 ± 0.475
0.816ProHis: 0.816 ± 0.265
1.923ProIle: 1.923 ± 0.299
2.797ProLys: 2.797 ± 0.452
2.622ProLeu: 2.622 ± 0.33
1.107ProMet: 1.107 ± 0.277
2.156ProAsn: 2.156 ± 0.397
1.748ProPro: 1.748 ± 0.344
1.166ProGln: 1.166 ± 0.255
1.399ProArg: 1.399 ± 0.263
2.797ProSer: 2.797 ± 0.407
3.089ProThr: 3.089 ± 0.506
3.322ProVal: 3.322 ± 0.505
0.466ProTrp: 0.466 ± 0.147
1.399ProTyr: 1.399 ± 0.334
0.0ProXaa: 0.0 ± 0.0
Gln
3.089GlnAla: 3.089 ± 0.456
0.0GlnCys: 0.0 ± 0.0
1.923GlnAsp: 1.923 ± 0.235
1.69GlnGlu: 1.69 ± 0.303
1.049GlnPhe: 1.049 ± 0.208
2.331GlnGly: 2.331 ± 0.443
0.932GlnHis: 0.932 ± 0.298
2.448GlnIle: 2.448 ± 0.356
2.797GlnLys: 2.797 ± 0.378
2.914GlnLeu: 2.914 ± 0.496
1.166GlnMet: 1.166 ± 0.222
1.632GlnAsn: 1.632 ± 0.354
1.049GlnPro: 1.049 ± 0.231
1.282GlnGln: 1.282 ± 0.255
1.515GlnArg: 1.515 ± 0.331
1.865GlnSer: 1.865 ± 0.357
1.807GlnThr: 1.807 ± 0.341
2.273GlnVal: 2.273 ± 0.406
0.408GlnTrp: 0.408 ± 0.127
1.573GlnTyr: 1.573 ± 0.27
0.0GlnXaa: 0.0 ± 0.0
Arg
4.021ArgAla: 4.021 ± 0.627
0.117ArgCys: 0.117 ± 0.087
3.089ArgAsp: 3.089 ± 0.397
2.855ArgGlu: 2.855 ± 0.423
1.632ArgPhe: 1.632 ± 0.316
2.914ArgGly: 2.914 ± 0.373
1.166ArgHis: 1.166 ± 0.329
2.739ArgIle: 2.739 ± 0.346
3.904ArgLys: 3.904 ± 0.581
4.545ArgLeu: 4.545 ± 0.499
1.107ArgMet: 1.107 ± 0.233
3.497ArgAsn: 3.497 ± 0.404
2.04ArgPro: 2.04 ± 0.398
1.632ArgGln: 1.632 ± 0.297
3.089ArgArg: 3.089 ± 0.638
2.331ArgSer: 2.331 ± 0.367
3.147ArgThr: 3.147 ± 0.441
3.205ArgVal: 3.205 ± 0.353
0.758ArgTrp: 0.758 ± 0.205
1.865ArgTyr: 1.865 ± 0.408
0.0ArgXaa: 0.0 ± 0.0
Ser
4.662SerAla: 4.662 ± 0.667
0.466SerCys: 0.466 ± 0.192
3.963SerAsp: 3.963 ± 0.557
3.497SerGlu: 3.497 ± 0.388
1.981SerPhe: 1.981 ± 0.343
4.953SerGly: 4.953 ± 0.527
1.049SerHis: 1.049 ± 0.252
4.021SerIle: 4.021 ± 0.362
3.497SerLys: 3.497 ± 0.616
4.72SerLeu: 4.72 ± 0.579
1.865SerMet: 1.865 ± 0.316
2.448SerAsn: 2.448 ± 0.415
2.855SerPro: 2.855 ± 0.512
1.865SerGln: 1.865 ± 0.336
3.38SerArg: 3.38 ± 0.54
4.079SerSer: 4.079 ± 0.469
4.021SerThr: 4.021 ± 0.715
3.03SerVal: 3.03 ± 0.409
1.282SerTrp: 1.282 ± 0.276
2.214SerTyr: 2.214 ± 0.37
0.0SerXaa: 0.0 ± 0.0
Thr
5.245ThrAla: 5.245 ± 0.854
0.35ThrCys: 0.35 ± 0.155
3.497ThrAsp: 3.497 ± 0.463
3.671ThrGlu: 3.671 ± 0.548
2.622ThrPhe: 2.622 ± 0.286
5.361ThrGly: 5.361 ± 0.649
0.991ThrHis: 0.991 ± 0.238
4.021ThrIle: 4.021 ± 0.554
4.604ThrLys: 4.604 ± 0.501
5.42ThrLeu: 5.42 ± 0.494
1.457ThrMet: 1.457 ± 0.375
2.506ThrAsn: 2.506 ± 0.495
3.671ThrPro: 3.671 ± 0.533
1.748ThrGln: 1.748 ± 0.281
2.914ThrArg: 2.914 ± 0.458
3.963ThrSer: 3.963 ± 0.534
4.779ThrThr: 4.779 ± 0.692
4.545ThrVal: 4.545 ± 0.65
0.758ThrTrp: 0.758 ± 0.269
2.214ThrTyr: 2.214 ± 0.408
0.0ThrXaa: 0.0 ± 0.0
Val
6.469ValAla: 6.469 ± 0.964
0.233ValCys: 0.233 ± 0.121
4.662ValAsp: 4.662 ± 0.534
4.72ValGlu: 4.72 ± 0.647
2.506ValPhe: 2.506 ± 0.398
5.128ValGly: 5.128 ± 0.625
1.049ValHis: 1.049 ± 0.295
4.312ValIle: 4.312 ± 0.461
5.07ValLys: 5.07 ± 0.452
5.711ValLeu: 5.711 ± 0.747
1.573ValMet: 1.573 ± 0.297
4.021ValAsn: 4.021 ± 0.457
2.855ValPro: 2.855 ± 0.468
2.506ValGln: 2.506 ± 0.409
3.613ValArg: 3.613 ± 0.583
3.788ValSer: 3.788 ± 0.533
4.138ValThr: 4.138 ± 0.53
5.07ValVal: 5.07 ± 0.686
1.224ValTrp: 1.224 ± 0.349
2.331ValTyr: 2.331 ± 0.477
0.0ValXaa: 0.0 ± 0.0
Trp
1.34TrpAla: 1.34 ± 0.258
0.117TrpCys: 0.117 ± 0.089
1.166TrpAsp: 1.166 ± 0.273
0.758TrpGlu: 0.758 ± 0.232
0.699TrpPhe: 0.699 ± 0.241
1.515TrpGly: 1.515 ± 0.332
0.233TrpHis: 0.233 ± 0.107
0.641TrpIle: 0.641 ± 0.226
1.049TrpLys: 1.049 ± 0.312
1.166TrpLeu: 1.166 ± 0.274
0.291TrpMet: 0.291 ± 0.12
0.991TrpAsn: 0.991 ± 0.274
0.291TrpPro: 0.291 ± 0.132
0.699TrpGln: 0.699 ± 0.318
0.758TrpArg: 0.758 ± 0.317
1.224TrpSer: 1.224 ± 0.334
0.874TrpThr: 0.874 ± 0.221
1.107TrpVal: 1.107 ± 0.229
0.291TrpTrp: 0.291 ± 0.162
0.524TrpTyr: 0.524 ± 0.217
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.03TyrAla: 3.03 ± 0.375
0.466TyrCys: 0.466 ± 0.169
2.681TyrAsp: 2.681 ± 0.43
1.981TyrGlu: 1.981 ± 0.408
1.632TyrPhe: 1.632 ± 0.332
3.671TyrGly: 3.671 ± 0.404
0.466TyrHis: 0.466 ± 0.173
1.399TyrIle: 1.399 ± 0.322
2.564TyrLys: 2.564 ± 0.449
2.273TyrLeu: 2.273 ± 0.34
0.583TyrMet: 0.583 ± 0.186
1.34TyrAsn: 1.34 ± 0.222
1.107TyrPro: 1.107 ± 0.246
1.107TyrGln: 1.107 ± 0.299
1.748TyrArg: 1.748 ± 0.416
2.098TyrSer: 2.098 ± 0.3
2.156TyrThr: 2.156 ± 0.509
2.972TyrVal: 2.972 ± 0.47
0.466TyrTrp: 0.466 ± 0.182
0.874TyrTyr: 0.874 ± 0.297
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 84 proteins (17161 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski