Nostoc punctiforme (strain ATCC 29133 / PCC 73102)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Nostocaceae; Nostoc; Nostoc punctiforme

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6573 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2IT80|B2IT80_NOSP7 Aldehyde dehydrogenase OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) OX=63737 GN=Npun_F0840 PE=3 SV=1
MM1 pKa = 7.75SDD3 pKa = 3.72NIFFAALNALTLNGCPSEE21 pKa = 4.24LAQSAASIVANDD33 pKa = 4.77DD34 pKa = 3.32PSKK37 pKa = 10.67PDD39 pKa = 3.41LGRR42 pKa = 11.84TTEE45 pKa = 4.05DD46 pKa = 3.03QQIIQEE52 pKa = 4.33TLPYY56 pKa = 10.12LQNQTWDD63 pKa = 3.17STGAYY68 pKa = 9.97DD69 pKa = 4.96SYY71 pKa = 10.93QTIDD75 pKa = 3.99DD76 pKa = 4.2TPHH79 pKa = 6.99PLAHH83 pKa = 6.08GQFLANLGGLVPDD96 pKa = 4.04NLIFALAEE104 pKa = 4.3GEE106 pKa = 4.57DD107 pKa = 4.02YY108 pKa = 10.84PFQVMQEE115 pKa = 4.06LGISEE120 pKa = 4.6DD121 pKa = 3.56VIEE124 pKa = 4.43RR125 pKa = 11.84AEE127 pKa = 4.4SIQSRR132 pKa = 11.84LGEE135 pKa = 3.83MNYY138 pKa = 9.74WGEE141 pKa = 3.97

Molecular weight: 15.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2J0S0|B2J0S0_NOSP7 Uncharacterized protein OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) OX=63737 GN=Npun_F1602 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 8.86RR3 pKa = 11.84TLGGTSRR10 pKa = 11.84KK11 pKa = 9.47RR12 pKa = 11.84KK13 pKa = 7.16RR14 pKa = 11.84TSGFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TPDD26 pKa = 3.17GRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.01KK37 pKa = 9.6GRR39 pKa = 11.84HH40 pKa = 5.0RR41 pKa = 11.84LSVV44 pKa = 3.12

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6573

0

6573

2290963

13

5352

348.5

38.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.071 ± 0.03

0.945 ± 0.011

4.868 ± 0.026

6.301 ± 0.03

3.967 ± 0.017

6.609 ± 0.037

1.785 ± 0.013

6.879 ± 0.024

4.956 ± 0.029

11.026 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.732 ± 0.015

4.557 ± 0.031

4.564 ± 0.027

5.496 ± 0.032

4.932 ± 0.025

6.612 ± 0.029

5.708 ± 0.027

6.566 ± 0.024

1.384 ± 0.011

3.044 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski