Bacteroides cellulosilyticus CAG:158

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; environmental samples

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4369 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6KJA5|R6KJA5_9BACE Uncharacterized protein OS=Bacteroides cellulosilyticus CAG:158 OX=1263038 GN=BN506_00690 PE=4 SV=1
MM1 pKa = 7.56LAMMMCMTALTFSACSDD18 pKa = 4.23DD19 pKa = 5.93DD20 pKa = 6.28DD21 pKa = 5.54DD22 pKa = 6.04DD23 pKa = 5.96KK24 pKa = 11.99SIDD27 pKa = 3.49VPEE30 pKa = 4.82AVTQALKK37 pKa = 10.48QKK39 pKa = 9.98YY40 pKa = 9.2PNATDD45 pKa = 3.76VDD47 pKa = 4.08WKK49 pKa = 10.74QKK51 pKa = 10.41SSYY54 pKa = 10.18FVADD58 pKa = 3.41CWLDD62 pKa = 3.86GKK64 pKa = 10.71DD65 pKa = 3.2SDD67 pKa = 4.55IWFDD71 pKa = 4.8ANGNWWMTEE80 pKa = 3.86SEE82 pKa = 4.57IYY84 pKa = 9.99WNDD87 pKa = 2.88VPGAVQTAFNNSEE100 pKa = 4.01YY101 pKa = 11.21ANWVQDD107 pKa = 3.45DD108 pKa = 5.49YY109 pKa = 12.15YY110 pKa = 11.47FLLYY114 pKa = 9.72PLQPMQYY121 pKa = 9.8VIEE124 pKa = 4.35VKK126 pKa = 10.43QGNQKK131 pKa = 9.48YY132 pKa = 8.17QLFYY136 pKa = 11.4SEE138 pKa = 5.93DD139 pKa = 3.57GGLMNTVNVTGKK151 pKa = 10.26DD152 pKa = 3.33DD153 pKa = 4.12TIYY156 pKa = 10.69PPEE159 pKa = 3.96EE160 pKa = 3.78

Molecular weight: 18.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6KGY6|R6KGY6_9BACE Uncharacterized protein OS=Bacteroides cellulosilyticus CAG:158 OX=1263038 GN=BN506_01824 PE=4 SV=1
MM1 pKa = 7.75IDD3 pKa = 3.33NGRR6 pKa = 11.84KK7 pKa = 9.38RR8 pKa = 11.84IIPNEE13 pKa = 3.82VLLPEE18 pKa = 4.23VARR21 pKa = 11.84LISEE25 pKa = 4.23GHH27 pKa = 5.27TVTLTVRR34 pKa = 11.84GNSMNPFLVDD44 pKa = 2.94RR45 pKa = 11.84RR46 pKa = 11.84DD47 pKa = 3.78RR48 pKa = 11.84IILGPFTDD56 pKa = 5.81DD57 pKa = 3.96DD58 pKa = 4.35LQPGVAVLARR68 pKa = 11.84DD69 pKa = 3.56TNGRR73 pKa = 11.84IIFHH77 pKa = 6.96RR78 pKa = 11.84IIHH81 pKa = 6.38RR82 pKa = 11.84NGQEE86 pKa = 3.76LTLLGDD92 pKa = 3.45GNIRR96 pKa = 11.84ITEE99 pKa = 3.85QTNIANVMGVMIAAIRR115 pKa = 11.84KK116 pKa = 7.96EE117 pKa = 3.96KK118 pKa = 10.33EE119 pKa = 3.94YY120 pKa = 10.52PCNDD124 pKa = 3.38RR125 pKa = 11.84VWQRR129 pKa = 11.84YY130 pKa = 6.84SFWWMKK136 pKa = 8.1LTPVRR141 pKa = 11.84RR142 pKa = 11.84WLLAIFRR149 pKa = 11.84RR150 pKa = 11.84RR151 pKa = 11.84KK152 pKa = 9.31IFHH155 pKa = 6.47PQMTRR160 pKa = 11.84ITQDD164 pKa = 2.47

Molecular weight: 19.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4369

0

4369

1722108

29

2312

394.2

44.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.966 ± 0.035

1.233 ± 0.014

5.531 ± 0.024

6.488 ± 0.032

4.555 ± 0.021

6.945 ± 0.031

1.858 ± 0.013

6.76 ± 0.032

6.403 ± 0.024

9.1 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.733 ± 0.017

5.207 ± 0.031

3.81 ± 0.019

3.493 ± 0.018

4.555 ± 0.019

6.196 ± 0.028

5.693 ± 0.028

6.381 ± 0.027

1.444 ± 0.015

4.648 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski