Roseovarius marisflavi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseovarius

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3958 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M7ALW3|A0A1M7ALW3_9RHOB Cu2+-exporting ATPase OS=Roseovarius marisflavi OX=1054996 GN=SAMN05444414_11432 PE=3 SV=1
MM1 pKa = 7.3SLKK4 pKa = 10.2TNIFMGALFASMPLALCAQDD24 pKa = 3.86AGGAFTLGYY33 pKa = 9.57GASSVSGPAGDD44 pKa = 5.4LSTLSLDD51 pKa = 3.66GVGNIALNNSFNIGLYY67 pKa = 10.41GSFRR71 pKa = 11.84SVDD74 pKa = 3.66EE75 pKa = 4.76NNTSDD80 pKa = 5.52DD81 pKa = 3.7LAASDD86 pKa = 5.62FGFTLNYY93 pKa = 9.94QFLNGVVLGGYY104 pKa = 9.89LDD106 pKa = 3.82YY107 pKa = 11.56AKK109 pKa = 10.9LDD111 pKa = 3.84FGSFATDD118 pKa = 4.16FDD120 pKa = 4.08TTSYY124 pKa = 10.67GGTVGYY130 pKa = 7.96VTNTFGAEE138 pKa = 3.73AFLGGTEE145 pKa = 4.12TSPDD149 pKa = 3.22LAAGVDD155 pKa = 3.39WVDD158 pKa = 3.06YY159 pKa = 10.47GLNLRR164 pKa = 11.84YY165 pKa = 9.67VVSPQARR172 pKa = 11.84LGGHH176 pKa = 6.24IMRR179 pKa = 11.84SDD181 pKa = 2.9ISVPGGTDD189 pKa = 3.35VEE191 pKa = 4.2IDD193 pKa = 3.45SYY195 pKa = 11.84GIGGDD200 pKa = 3.62YY201 pKa = 11.14AFGNGWSGFGGLSYY215 pKa = 11.4VDD217 pKa = 4.5LGAAAVDD224 pKa = 3.45ATTFGVGVGYY234 pKa = 10.49DD235 pKa = 3.55LGQISRR241 pKa = 11.84LPASVSLEE249 pKa = 3.8LSRR252 pKa = 11.84TDD254 pKa = 3.07ISAPGADD261 pKa = 3.71ADD263 pKa = 3.93VDD265 pKa = 4.03TLRR268 pKa = 11.84LSLTVPLGNRR278 pKa = 11.84PSTTPLNSVARR289 pKa = 11.84SAMAPRR295 pKa = 11.84HH296 pKa = 5.35NAVSTLLEE304 pKa = 4.15SAFF307 pKa = 3.84

Molecular weight: 31.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M7AE79|A0A1M7AE79_9RHOB Uncharacterized protein OS=Roseovarius marisflavi OX=1054996 GN=SAMN05444414_11348 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.43EE41 pKa = 3.72LSAA44 pKa = 5.03

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3958

0

3958

1212272

24

9929

306.3

33.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.116 ± 0.051

0.947 ± 0.016

6.048 ± 0.05

5.75 ± 0.033

3.711 ± 0.023

8.803 ± 0.063

2.115 ± 0.025

5.33 ± 0.029

3.269 ± 0.03

10.038 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.869 ± 0.026

2.659 ± 0.031

4.994 ± 0.035

3.148 ± 0.021

6.629 ± 0.052

5.218 ± 0.034

5.485 ± 0.048

7.241 ± 0.032

1.375 ± 0.019

2.256 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski