Amino acid dipepetide frequency for Flavonifractor sp. An100

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.48AlaAla: 10.48 ± 0.153
1.474AlaCys: 1.474 ± 0.045
4.752AlaAsp: 4.752 ± 0.087
5.449AlaGlu: 5.449 ± 0.099
3.168AlaPhe: 3.168 ± 0.063
7.533AlaGly: 7.533 ± 0.113
1.509AlaHis: 1.509 ± 0.043
5.149AlaIle: 5.149 ± 0.079
4.157AlaLys: 4.157 ± 0.084
10.26AlaLeu: 10.26 ± 0.143
2.816AlaMet: 2.816 ± 0.054
2.412AlaAsn: 2.412 ± 0.062
3.554AlaPro: 3.554 ± 0.088
4.298AlaGln: 4.298 ± 0.074
4.424AlaArg: 4.424 ± 0.075
4.395AlaSer: 4.395 ± 0.077
3.923AlaThr: 3.923 ± 0.079
7.474AlaVal: 7.474 ± 0.092
0.963AlaTrp: 0.963 ± 0.038
2.74AlaTyr: 2.74 ± 0.064
0.0AlaXaa: 0.0 ± 0.0
Cys
1.486CysAla: 1.486 ± 0.042
0.356CysCys: 0.356 ± 0.02
0.821CysAsp: 0.821 ± 0.031
0.724CysGlu: 0.724 ± 0.032
0.589CysPhe: 0.589 ± 0.026
1.691CysGly: 1.691 ± 0.048
0.356CysHis: 0.356 ± 0.021
0.754CysIle: 0.754 ± 0.027
0.602CysLys: 0.602 ± 0.027
1.534CysLeu: 1.534 ± 0.047
0.364CysMet: 0.364 ± 0.02
0.439CysAsn: 0.439 ± 0.022
0.839CysPro: 0.839 ± 0.036
0.647CysGln: 0.647 ± 0.029
1.048CysArg: 1.048 ± 0.036
0.952CysSer: 0.952 ± 0.034
0.843CysThr: 0.843 ± 0.027
1.146CysVal: 1.146 ± 0.035
0.206CysTrp: 0.206 ± 0.015
0.558CysTyr: 0.558 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
4.353AspAla: 4.353 ± 0.083
0.858AspCys: 0.858 ± 0.029
2.482AspAsp: 2.482 ± 0.078
3.552AspGlu: 3.552 ± 0.081
2.262AspPhe: 2.262 ± 0.046
4.787AspGly: 4.787 ± 0.084
1.13AspHis: 1.13 ± 0.034
3.102AspIle: 3.102 ± 0.061
2.448AspLys: 2.448 ± 0.063
5.525AspLeu: 5.525 ± 0.072
1.504AspMet: 1.504 ± 0.043
1.621AspAsn: 1.621 ± 0.047
2.768AspPro: 2.768 ± 0.065
2.111AspGln: 2.111 ± 0.054
3.181AspArg: 3.181 ± 0.062
2.807AspSer: 2.807 ± 0.058
3.082AspThr: 3.082 ± 0.066
3.514AspVal: 3.514 ± 0.062
0.804AspTrp: 0.804 ± 0.038
2.381AspTyr: 2.381 ± 0.06
0.0AspXaa: 0.0 ± 0.0
Glu
5.845GluAla: 5.845 ± 0.1
0.737GluCys: 0.737 ± 0.031
3.822GluAsp: 3.822 ± 0.076
6.681GluGlu: 6.681 ± 0.118
2.039GluPhe: 2.039 ± 0.052
5.12GluGly: 5.12 ± 0.085
1.389GluHis: 1.389 ± 0.04
3.792GluIle: 3.792 ± 0.08
4.014GluLys: 4.014 ± 0.08
7.053GluLeu: 7.053 ± 0.115
1.924GluMet: 1.924 ± 0.05
2.687GluAsn: 2.687 ± 0.061
2.216GluPro: 2.216 ± 0.06
3.57GluGln: 3.57 ± 0.076
4.296GluArg: 4.296 ± 0.077
2.869GluSer: 2.869 ± 0.062
3.185GluThr: 3.185 ± 0.063
4.211GluVal: 4.211 ± 0.069
0.692GluTrp: 0.692 ± 0.027
2.255GluTyr: 2.255 ± 0.058
0.0GluXaa: 0.0 ± 0.0
Phe
3.014PheAla: 3.014 ± 0.061
0.725PheCys: 0.725 ± 0.03
2.191PheAsp: 2.191 ± 0.051
1.951PheGlu: 1.951 ± 0.044
1.643PhePhe: 1.643 ± 0.05
3.044PheGly: 3.044 ± 0.073
0.877PheHis: 0.877 ± 0.035
1.781PheIle: 1.781 ± 0.053
1.155PheLys: 1.155 ± 0.037
3.944PheLeu: 3.944 ± 0.079
0.78PheMet: 0.78 ± 0.027
1.086PheAsn: 1.086 ± 0.04
1.647PhePro: 1.647 ± 0.046
1.605PheGln: 1.605 ± 0.042
1.947PheArg: 1.947 ± 0.05
2.714PheSer: 2.714 ± 0.063
2.277PheThr: 2.277 ± 0.055
2.421PheVal: 2.421 ± 0.063
0.459PheTrp: 0.459 ± 0.022
1.353PheTyr: 1.353 ± 0.041
0.0PheXaa: 0.0 ± 0.0
Gly
6.524GlyAla: 6.524 ± 0.105
1.472GlyCys: 1.472 ± 0.048
4.104GlyAsp: 4.104 ± 0.078
5.419GlyGlu: 5.419 ± 0.091
2.877GlyPhe: 2.877 ± 0.061
6.481GlyGly: 6.481 ± 0.126
1.475GlyHis: 1.475 ± 0.041
4.691GlyIle: 4.691 ± 0.083
4.525GlyLys: 4.525 ± 0.077
7.583GlyLeu: 7.583 ± 0.109
2.634GlyMet: 2.634 ± 0.061
2.531GlyAsn: 2.531 ± 0.056
2.196GlyPro: 2.196 ± 0.052
2.931GlyGln: 2.931 ± 0.067
4.49GlyArg: 4.49 ± 0.083
4.665GlySer: 4.665 ± 0.091
4.799GlyThr: 4.799 ± 0.087
6.037GlyVal: 6.037 ± 0.101
0.998GlyTrp: 0.998 ± 0.038
3.077GlyTyr: 3.077 ± 0.066
0.0GlyXaa: 0.0 ± 0.0
His
1.294HisAla: 1.294 ± 0.039
0.4HisCys: 0.4 ± 0.021
0.86HisAsp: 0.86 ± 0.032
0.929HisGlu: 0.929 ± 0.035
0.773HisPhe: 0.773 ± 0.031
1.443HisGly: 1.443 ± 0.044
0.436HisHis: 0.436 ± 0.026
1.277HisIle: 1.277 ± 0.039
0.726HisLys: 0.726 ± 0.027
1.884HisLeu: 1.884 ± 0.051
0.538HisMet: 0.538 ± 0.025
0.636HisAsn: 0.636 ± 0.026
1.207HisPro: 1.207 ± 0.038
0.704HisGln: 0.704 ± 0.029
1.117HisArg: 1.117 ± 0.039
1.009HisSer: 1.009 ± 0.036
1.183HisThr: 1.183 ± 0.039
1.083HisVal: 1.083 ± 0.038
0.267HisTrp: 0.267 ± 0.017
0.736HisTyr: 0.736 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
5.001IleAla: 5.001 ± 0.086
0.995IleCys: 0.995 ± 0.033
2.977IleAsp: 2.977 ± 0.055
2.809IleGlu: 2.809 ± 0.066
2.163IlePhe: 2.163 ± 0.064
3.995IleGly: 3.995 ± 0.08
1.09IleHis: 1.09 ± 0.035
2.967IleIle: 2.967 ± 0.068
2.14IleLys: 2.14 ± 0.059
5.874IleLeu: 5.874 ± 0.082
1.26IleMet: 1.26 ± 0.04
1.806IleAsn: 1.806 ± 0.05
3.036IlePro: 3.036 ± 0.062
2.354IleGln: 2.354 ± 0.048
3.359IleArg: 3.359 ± 0.059
3.648IleSer: 3.648 ± 0.075
3.517IleThr: 3.517 ± 0.072
3.628IleVal: 3.628 ± 0.076
0.56IleTrp: 0.56 ± 0.026
1.851IleTyr: 1.851 ± 0.054
0.0IleXaa: 0.0 ± 0.0
Lys
4.376LysAla: 4.376 ± 0.09
0.48LysCys: 0.48 ± 0.026
2.467LysAsp: 2.467 ± 0.056
3.811LysGlu: 3.811 ± 0.079
1.198LysPhe: 1.198 ± 0.04
3.463LysGly: 3.463 ± 0.063
0.76LysHis: 0.76 ± 0.028
2.531LysIle: 2.531 ± 0.06
3.25LysLys: 3.25 ± 0.078
4.467LysLeu: 4.467 ± 0.08
1.281LysMet: 1.281 ± 0.042
1.837LysAsn: 1.837 ± 0.05
1.881LysPro: 1.881 ± 0.044
1.868LysGln: 1.868 ± 0.05
2.867LysArg: 2.867 ± 0.063
2.299LysSer: 2.299 ± 0.052
2.689LysThr: 2.689 ± 0.058
3.248LysVal: 3.248 ± 0.06
0.474LysTrp: 0.474 ± 0.022
1.552LysTyr: 1.552 ± 0.047
0.0LysXaa: 0.0 ± 0.0
Leu
9.686LeuAla: 9.686 ± 0.126
1.966LeuCys: 1.966 ± 0.048
5.837LeuAsp: 5.837 ± 0.068
7.45LeuGlu: 7.45 ± 0.104
3.848LeuPhe: 3.848 ± 0.084
7.592LeuGly: 7.592 ± 0.107
1.719LeuHis: 1.719 ± 0.048
5.062LeuIle: 5.062 ± 0.094
4.358LeuLys: 4.358 ± 0.08
10.49LeuLeu: 10.49 ± 0.156
2.669LeuMet: 2.669 ± 0.055
3.209LeuAsn: 3.209 ± 0.05
4.964LeuPro: 4.964 ± 0.085
3.271LeuGln: 3.271 ± 0.069
5.837LeuArg: 5.837 ± 0.101
7.311LeuSer: 7.311 ± 0.114
6.199LeuThr: 6.199 ± 0.097
6.648LeuVal: 6.648 ± 0.104
1.14LeuTrp: 1.14 ± 0.039
3.189LeuTyr: 3.189 ± 0.063
0.0LeuXaa: 0.0 ± 0.0
Met
2.778MetAla: 2.778 ± 0.058
0.317MetCys: 0.317 ± 0.02
1.809MetAsp: 1.809 ± 0.045
2.403MetGlu: 2.403 ± 0.046
0.809MetPhe: 0.809 ± 0.032
2.273MetGly: 2.273 ± 0.049
0.352MetHis: 0.352 ± 0.022
1.347MetIle: 1.347 ± 0.041
1.649MetLys: 1.649 ± 0.045
2.58MetLeu: 2.58 ± 0.06
0.734MetMet: 0.734 ± 0.029
1.053MetAsn: 1.053 ± 0.035
1.112MetPro: 1.112 ± 0.033
0.728MetGln: 0.728 ± 0.029
1.375MetArg: 1.375 ± 0.039
1.686MetSer: 1.686 ± 0.04
1.628MetThr: 1.628 ± 0.038
1.984MetVal: 1.984 ± 0.048
0.21MetTrp: 0.21 ± 0.017
0.623MetTyr: 0.623 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
2.7AsnAla: 2.7 ± 0.06
0.514AsnCys: 0.514 ± 0.027
1.483AsnAsp: 1.483 ± 0.039
1.801AsnGlu: 1.801 ± 0.046
1.181AsnPhe: 1.181 ± 0.034
2.78AsnGly: 2.78 ± 0.058
0.647AsnHis: 0.647 ± 0.028
2.054AsnIle: 2.054 ± 0.048
1.359AsnLys: 1.359 ± 0.048
3.342AsnLeu: 3.342 ± 0.061
0.894AsnMet: 0.894 ± 0.034
1.03AsnAsn: 1.03 ± 0.04
1.866AsnPro: 1.866 ± 0.048
1.339AsnGln: 1.339 ± 0.035
1.914AsnArg: 1.914 ± 0.052
1.652AsnSer: 1.652 ± 0.05
1.899AsnThr: 1.899 ± 0.05
2.143AsnVal: 2.143 ± 0.052
0.38AsnTrp: 0.38 ± 0.019
1.235AsnTyr: 1.235 ± 0.034
0.0AsnXaa: 0.0 ± 0.0
Pro
3.981ProAla: 3.981 ± 0.079
0.592ProCys: 0.592 ± 0.029
2.871ProAsp: 2.871 ± 0.052
3.825ProGlu: 3.825 ± 0.067
1.596ProPhe: 1.596 ± 0.048
3.399ProGly: 3.399 ± 0.074
0.842ProHis: 0.842 ± 0.033
2.262ProIle: 2.262 ± 0.054
1.917ProLys: 1.917 ± 0.043
3.853ProLeu: 3.853 ± 0.079
1.185ProMet: 1.185 ± 0.04
1.507ProAsn: 1.507 ± 0.045
1.697ProPro: 1.697 ± 0.046
1.924ProGln: 1.924 ± 0.044
1.848ProArg: 1.848 ± 0.046
2.482ProSer: 2.482 ± 0.048
2.132ProThr: 2.132 ± 0.048
3.64ProVal: 3.64 ± 0.069
0.526ProTrp: 0.526 ± 0.027
1.483ProTyr: 1.483 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
4.499GlnAla: 4.499 ± 0.079
0.442GlnCys: 0.442 ± 0.023
1.918GlnAsp: 1.918 ± 0.054
3.31GlnGlu: 3.31 ± 0.068
1.244GlnPhe: 1.244 ± 0.04
3.016GlnGly: 3.016 ± 0.064
0.608GlnHis: 0.608 ± 0.028
2.275GlnIle: 2.275 ± 0.048
2.019GlnLys: 2.019 ± 0.059
4.064GlnLeu: 4.064 ± 0.073
1.139GlnMet: 1.139 ± 0.04
1.333GlnAsn: 1.333 ± 0.038
1.646GlnPro: 1.646 ± 0.048
1.838GlnGln: 1.838 ± 0.051
2.486GlnArg: 2.486 ± 0.054
2.138GlnSer: 2.138 ± 0.047
2.164GlnThr: 2.164 ± 0.059
3.3GlnVal: 3.3 ± 0.067
0.5GlnTrp: 0.5 ± 0.028
1.342GlnTyr: 1.342 ± 0.04
0.0GlnXaa: 0.0 ± 0.0
Arg
4.405ArgAla: 4.405 ± 0.08
0.892ArgCys: 0.892 ± 0.032
2.963ArgAsp: 2.963 ± 0.064
4.455ArgGlu: 4.455 ± 0.092
2.099ArgPhe: 2.099 ± 0.053
3.628ArgGly: 3.628 ± 0.07
1.048ArgHis: 1.048 ± 0.032
3.037ArgIle: 3.037 ± 0.066
2.934ArgLys: 2.934 ± 0.058
5.847ArgLeu: 5.847 ± 0.103
1.661ArgMet: 1.661 ± 0.044
1.806ArgAsn: 1.806 ± 0.046
2.41ArgPro: 2.41 ± 0.054
2.803ArgGln: 2.803 ± 0.07
4.088ArgArg: 4.088 ± 0.086
3.052ArgSer: 3.052 ± 0.065
2.902ArgThr: 2.902 ± 0.072
3.64ArgVal: 3.64 ± 0.071
0.758ArgTrp: 0.758 ± 0.039
2.099ArgTyr: 2.099 ± 0.045
0.0ArgXaa: 0.0 ± 0.0
Ser
5.127SerAla: 5.127 ± 0.083
0.832SerCys: 0.832 ± 0.026
2.881SerAsp: 2.881 ± 0.066
2.926SerGlu: 2.926 ± 0.06
2.441SerPhe: 2.441 ± 0.049
5.487SerGly: 5.487 ± 0.09
1.139SerHis: 1.139 ± 0.038
3.154SerIle: 3.154 ± 0.073
2.356SerLys: 2.356 ± 0.051
5.764SerLeu: 5.764 ± 0.09
1.564SerMet: 1.564 ± 0.038
1.747SerAsn: 1.747 ± 0.044
2.559SerPro: 2.559 ± 0.061
2.644SerGln: 2.644 ± 0.058
3.239SerArg: 3.239 ± 0.066
3.762SerSer: 3.762 ± 0.11
3.013SerThr: 3.013 ± 0.058
4.057SerVal: 4.057 ± 0.075
0.657SerTrp: 0.657 ± 0.03
2.058SerTyr: 2.058 ± 0.05
0.0SerXaa: 0.0 ± 0.0
Thr
5.429ThrAla: 5.429 ± 0.087
0.76ThrCys: 0.76 ± 0.027
3.009ThrAsp: 3.009 ± 0.064
2.948ThrGlu: 2.948 ± 0.07
2.078ThrPhe: 2.078 ± 0.053
5.062ThrGly: 5.062 ± 0.089
1.047ThrHis: 1.047 ± 0.033
3.392ThrIle: 3.392 ± 0.078
2.195ThrLys: 2.195 ± 0.056
5.838ThrLeu: 5.838 ± 0.089
1.459ThrMet: 1.459 ± 0.042
1.549ThrAsn: 1.549 ± 0.043
2.969ThrPro: 2.969 ± 0.059
2.175ThrGln: 2.175 ± 0.053
2.555ThrArg: 2.555 ± 0.056
2.71ThrSer: 2.71 ± 0.056
2.948ThrThr: 2.948 ± 0.064
4.874ThrVal: 4.874 ± 0.089
0.623ThrTrp: 0.623 ± 0.027
1.908ThrTyr: 1.908 ± 0.054
0.0ThrXaa: 0.0 ± 0.0
Val
6.197ValAla: 6.197 ± 0.105
1.313ValCys: 1.313 ± 0.045
4.146ValAsp: 4.146 ± 0.069
4.947ValGlu: 4.947 ± 0.078
2.726ValPhe: 2.726 ± 0.064
5.116ValGly: 5.116 ± 0.096
1.141ValHis: 1.141 ± 0.037
3.997ValIle: 3.997 ± 0.073
3.158ValLys: 3.158 ± 0.062
7.644ValLeu: 7.644 ± 0.112
1.976ValMet: 1.976 ± 0.045
2.254ValAsn: 2.254 ± 0.053
3.154ValPro: 3.154 ± 0.064
2.289ValGln: 2.289 ± 0.054
3.678ValArg: 3.678 ± 0.072
4.569ValSer: 4.569 ± 0.069
4.461ValThr: 4.461 ± 0.073
5.437ValVal: 5.437 ± 0.098
0.859ValTrp: 0.859 ± 0.034
2.329ValTyr: 2.329 ± 0.047
0.0ValXaa: 0.0 ± 0.0
Trp
0.951TrpAla: 0.951 ± 0.035
0.216TrpCys: 0.216 ± 0.017
0.675TrpAsp: 0.675 ± 0.028
0.872TrpGlu: 0.872 ± 0.033
0.473TrpPhe: 0.473 ± 0.02
0.876TrpGly: 0.876 ± 0.034
0.186TrpHis: 0.186 ± 0.014
0.508TrpIle: 0.508 ± 0.024
0.589TrpLys: 0.589 ± 0.029
1.328TrpLeu: 1.328 ± 0.045
0.331TrpMet: 0.331 ± 0.018
0.472TrpAsn: 0.472 ± 0.025
0.405TrpPro: 0.405 ± 0.022
0.537TrpGln: 0.537 ± 0.027
0.706TrpArg: 0.706 ± 0.035
0.668TrpSer: 0.668 ± 0.028
0.548TrpThr: 0.548 ± 0.031
0.716TrpVal: 0.716 ± 0.035
0.134TrpTrp: 0.134 ± 0.013
0.4TrpTyr: 0.4 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.803TyrAla: 2.803 ± 0.051
0.617TyrCys: 0.617 ± 0.027
2.203TyrAsp: 2.203 ± 0.055
2.134TyrGlu: 2.134 ± 0.049
1.407TyrPhe: 1.407 ± 0.041
2.723TyrGly: 2.723 ± 0.052
0.754TyrHis: 0.754 ± 0.034
1.899TyrIle: 1.899 ± 0.045
1.247TyrLys: 1.247 ± 0.038
3.571TyrLeu: 3.571 ± 0.059
0.734TyrMet: 0.734 ± 0.03
1.199TyrAsn: 1.199 ± 0.035
1.436TyrPro: 1.436 ± 0.043
1.646TyrGln: 1.646 ± 0.046
2.071TyrArg: 2.071 ± 0.058
2.019TyrSer: 2.019 ± 0.057
2.136TyrThr: 2.136 ± 0.065
2.191TyrVal: 2.191 ± 0.048
0.379TyrTrp: 0.379 ± 0.023
1.426TyrTyr: 1.426 ± 0.049
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2837 proteins (881498 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski