Pseudomonas phage PPpW-4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Phutvirus; Pseudomonas virus PPpW4

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5YSZ1|V5YSZ1_9CAUD Putative nucleotidyl transferase OS=Pseudomonas phage PPpW-4 OX=1279083 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.58FSSFNLAAGFNAVLSHH19 pKa = 6.9LNAEE23 pKa = 4.38EE24 pKa = 4.41NEE26 pKa = 4.19MAAEE30 pKa = 3.97FMEE33 pKa = 4.33VLKK36 pKa = 10.8DD37 pKa = 3.59AQKK40 pKa = 11.15DD41 pKa = 3.89VAHH44 pKa = 7.1LNTLQNRR51 pKa = 11.84GVDD54 pKa = 3.13NWDD57 pKa = 3.67GYY59 pKa = 10.84VGPGCEE65 pKa = 4.11SCPEE69 pKa = 4.39CGDD72 pKa = 4.37DD73 pKa = 4.33VDD75 pKa = 4.09EE76 pKa = 5.18TEE78 pKa = 4.46FGPNGVCLDD87 pKa = 4.22CEE89 pKa = 4.28HH90 pKa = 7.36GEE92 pKa = 5.04GGDD95 pKa = 3.42QQ96 pKa = 4.2

Molecular weight: 10.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5YTH2|V5YTH2_9CAUD Uncharacterized protein OS=Pseudomonas phage PPpW-4 OX=1279083 PE=4 SV=1
MM1 pKa = 7.87EE2 pKa = 4.55IQMQVSVRR10 pKa = 11.84PARR13 pKa = 11.84IQSRR17 pKa = 11.84KK18 pKa = 8.66PRR20 pKa = 11.84LSEE23 pKa = 3.89TQGASTLFAGKK34 pKa = 8.47TDD36 pKa = 3.9FEE38 pKa = 4.87VGQEE42 pKa = 4.05RR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84KK46 pKa = 7.85DD47 pKa = 3.57HH48 pKa = 6.26KK49 pKa = 10.34PKK51 pKa = 10.12RR52 pKa = 11.84HH53 pKa = 6.31PRR55 pKa = 11.84TEE57 pKa = 3.49WEE59 pKa = 4.4YY60 pKa = 10.32PHH62 pKa = 6.96HH63 pKa = 6.2KK64 pKa = 9.42TNIGAEE70 pKa = 3.94KK71 pKa = 9.39GRR73 pKa = 11.84YY74 pKa = 7.78MGEE77 pKa = 3.96EE78 pKa = 3.97LL79 pKa = 4.9

Molecular weight: 9.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12566

44

1332

251.3

27.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.17 ± 0.519

1.003 ± 0.169

6.311 ± 0.203

6.43 ± 0.359

3.533 ± 0.16

8.308 ± 0.329

2.053 ± 0.189

4.87 ± 0.187

5.531 ± 0.421

8.101 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.857 ± 0.238

3.907 ± 0.284

3.947 ± 0.191

4.154 ± 0.329

5.905 ± 0.225

5.682 ± 0.314

5.77 ± 0.296

7.043 ± 0.329

1.393 ± 0.142

3.032 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski