Amino acid dipepetide frequency for Mycobacterium phage Weirdo19

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.677AlaAla: 23.677 ± 1.921
0.835AlaCys: 0.835 ± 0.24
7.694AlaAsp: 7.694 ± 0.808
9.543AlaGlu: 9.543 ± 1.009
3.698AlaPhe: 3.698 ± 0.431
12.644AlaGly: 12.644 ± 1.301
2.803AlaHis: 2.803 ± 0.435
5.368AlaIle: 5.368 ± 0.527
3.877AlaLys: 3.877 ± 0.64
10.735AlaLeu: 10.735 ± 0.782
3.578AlaMet: 3.578 ± 0.478
3.28AlaAsn: 3.28 ± 0.426
7.753AlaPro: 7.753 ± 0.972
3.519AlaGln: 3.519 ± 0.549
10.02AlaArg: 10.02 ± 0.806
6.203AlaSer: 6.203 ± 0.666
7.336AlaThr: 7.336 ± 0.821
8.827AlaVal: 8.827 ± 0.8
2.028AlaTrp: 2.028 ± 0.279
1.67AlaTyr: 1.67 ± 0.422
0.0AlaXaa: 0.0 ± 0.0
Cys
1.133CysAla: 1.133 ± 0.297
0.298CysCys: 0.298 ± 0.138
0.358CysAsp: 0.358 ± 0.149
0.716CysGlu: 0.716 ± 0.207
0.179CysPhe: 0.179 ± 0.101
1.193CysGly: 1.193 ± 0.33
0.477CysHis: 0.477 ± 0.18
0.358CysIle: 0.358 ± 0.141
0.298CysLys: 0.298 ± 0.124
0.477CysLeu: 0.477 ± 0.151
0.06CysMet: 0.06 ± 0.057
0.358CysAsn: 0.358 ± 0.157
0.656CysPro: 0.656 ± 0.274
0.537CysGln: 0.537 ± 0.161
1.372CysArg: 1.372 ± 0.325
0.477CysSer: 0.477 ± 0.176
0.477CysThr: 0.477 ± 0.168
0.477CysVal: 0.477 ± 0.154
0.239CysTrp: 0.239 ± 0.113
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
7.336AspAla: 7.336 ± 0.899
0.358AspCys: 0.358 ± 0.141
4.652AspAsp: 4.652 ± 0.664
4.652AspGlu: 4.652 ± 0.823
1.014AspPhe: 1.014 ± 0.238
6.143AspGly: 6.143 ± 0.666
1.372AspHis: 1.372 ± 0.29
2.326AspIle: 2.326 ± 0.352
1.67AspLys: 1.67 ± 0.347
5.785AspLeu: 5.785 ± 0.727
0.835AspMet: 0.835 ± 0.183
2.028AspAsn: 2.028 ± 0.333
5.189AspPro: 5.189 ± 0.635
1.73AspGln: 1.73 ± 0.293
4.592AspArg: 4.592 ± 0.63
1.431AspSer: 1.431 ± 0.337
4.235AspThr: 4.235 ± 0.405
3.996AspVal: 3.996 ± 0.517
1.133AspTrp: 1.133 ± 0.242
1.551AspTyr: 1.551 ± 0.274
0.0AspXaa: 0.0 ± 0.0
Glu
10.437GluAla: 10.437 ± 1.053
0.596GluCys: 0.596 ± 0.225
3.101GluAsp: 3.101 ± 0.531
2.743GluGlu: 2.743 ± 0.423
2.565GluPhe: 2.565 ± 0.486
3.638GluGly: 3.638 ± 0.471
1.789GluHis: 1.789 ± 0.291
1.551GluIle: 1.551 ± 0.323
0.656GluLys: 0.656 ± 0.196
6.62GluLeu: 6.62 ± 0.629
0.596GluMet: 0.596 ± 0.217
0.954GluAsn: 0.954 ± 0.301
3.698GluPro: 3.698 ± 0.621
2.087GluGln: 2.087 ± 0.35
4.533GluArg: 4.533 ± 0.587
1.551GluSer: 1.551 ± 0.298
3.578GluThr: 3.578 ± 0.481
5.248GluVal: 5.248 ± 0.551
1.312GluTrp: 1.312 ± 0.273
1.074GluTyr: 1.074 ± 0.197
0.0GluXaa: 0.0 ± 0.0
Phe
3.519PheAla: 3.519 ± 0.528
0.179PheCys: 0.179 ± 0.109
2.266PheAsp: 2.266 ± 0.4
1.73PheGlu: 1.73 ± 0.305
0.656PhePhe: 0.656 ± 0.205
2.565PheGly: 2.565 ± 0.359
0.537PheHis: 0.537 ± 0.168
1.074PheIle: 1.074 ± 0.275
0.656PheLys: 0.656 ± 0.171
1.193PheLeu: 1.193 ± 0.268
0.239PheMet: 0.239 ± 0.109
0.537PheAsn: 0.537 ± 0.225
1.372PhePro: 1.372 ± 0.28
0.835PheGln: 0.835 ± 0.354
1.849PheArg: 1.849 ± 0.332
1.789PheSer: 1.789 ± 0.398
2.386PheThr: 2.386 ± 0.354
2.087PheVal: 2.087 ± 0.342
0.358PheTrp: 0.358 ± 0.161
1.133PheTyr: 1.133 ± 0.255
0.0PheXaa: 0.0 ± 0.0
Gly
8.887GlyAla: 8.887 ± 1.074
0.895GlyCys: 0.895 ± 0.275
7.097GlyAsp: 7.097 ± 0.58
5.785GlyGlu: 5.785 ± 0.612
1.849GlyPhe: 1.849 ± 0.334
11.63GlyGly: 11.63 ± 1.741
2.147GlyHis: 2.147 ± 0.353
3.459GlyIle: 3.459 ± 0.568
3.101GlyLys: 3.101 ± 0.511
7.395GlyLeu: 7.395 ± 0.675
1.73GlyMet: 1.73 ± 0.285
2.743GlyAsn: 2.743 ± 0.461
6.262GlyPro: 6.262 ± 0.646
3.459GlyGln: 3.459 ± 0.495
7.395GlyArg: 7.395 ± 0.813
3.996GlySer: 3.996 ± 0.649
5.726GlyThr: 5.726 ± 0.631
8.35GlyVal: 8.35 ± 0.891
1.372GlyTrp: 1.372 ± 0.303
2.505GlyTyr: 2.505 ± 0.44
0.0GlyXaa: 0.0 ± 0.0
His
2.982HisAla: 2.982 ± 0.419
0.239HisCys: 0.239 ± 0.118
1.312HisAsp: 1.312 ± 0.265
1.252HisGlu: 1.252 ± 0.263
0.358HisPhe: 0.358 ± 0.128
1.61HisGly: 1.61 ± 0.284
0.954HisHis: 0.954 ± 0.299
0.835HisIle: 0.835 ± 0.266
0.537HisLys: 0.537 ± 0.163
2.445HisLeu: 2.445 ± 0.386
0.537HisMet: 0.537 ± 0.186
0.537HisAsn: 0.537 ± 0.174
2.266HisPro: 2.266 ± 0.421
0.417HisGln: 0.417 ± 0.153
2.624HisArg: 2.624 ± 0.501
0.716HisSer: 0.716 ± 0.205
1.252HisThr: 1.252 ± 0.272
1.193HisVal: 1.193 ± 0.287
0.358HisTrp: 0.358 ± 0.134
0.596HisTyr: 0.596 ± 0.202
0.0HisXaa: 0.0 ± 0.0
Ile
6.143IleAla: 6.143 ± 0.633
0.477IleCys: 0.477 ± 0.161
2.207IleAsp: 2.207 ± 0.345
3.101IleGlu: 3.101 ± 0.418
0.537IlePhe: 0.537 ± 0.161
3.4IleGly: 3.4 ± 0.454
0.716IleHis: 0.716 ± 0.212
1.372IleIle: 1.372 ± 0.307
1.014IleLys: 1.014 ± 0.332
2.147IleLeu: 2.147 ± 0.348
0.716IleMet: 0.716 ± 0.239
1.074IleAsn: 1.074 ± 0.27
3.519IlePro: 3.519 ± 0.531
0.656IleGln: 0.656 ± 0.208
3.042IleArg: 3.042 ± 0.355
1.312IleSer: 1.312 ± 0.345
2.922IleThr: 2.922 ± 0.366
3.28IleVal: 3.28 ± 0.45
0.298IleTrp: 0.298 ± 0.13
0.835IleTyr: 0.835 ± 0.243
0.0IleXaa: 0.0 ± 0.0
Lys
5.069LysAla: 5.069 ± 0.837
0.298LysCys: 0.298 ± 0.127
1.074LysAsp: 1.074 ± 0.326
1.014LysGlu: 1.014 ± 0.32
1.252LysPhe: 1.252 ± 0.255
1.67LysGly: 1.67 ± 0.43
0.417LysHis: 0.417 ± 0.162
0.895LysIle: 0.895 ± 0.222
0.596LysLys: 0.596 ± 0.219
2.803LysLeu: 2.803 ± 0.514
0.477LysMet: 0.477 ± 0.13
0.358LysAsn: 0.358 ± 0.123
1.372LysPro: 1.372 ± 0.331
0.775LysGln: 0.775 ± 0.226
1.67LysArg: 1.67 ± 0.3
0.835LysSer: 0.835 ± 0.272
1.133LysThr: 1.133 ± 0.295
2.087LysVal: 2.087 ± 0.324
0.358LysTrp: 0.358 ± 0.172
0.298LysTyr: 0.298 ± 0.123
0.0LysXaa: 0.0 ± 0.0
Leu
11.213LeuAla: 11.213 ± 0.929
0.775LeuCys: 0.775 ± 0.22
7.813LeuAsp: 7.813 ± 0.506
2.266LeuGlu: 2.266 ± 0.315
1.968LeuPhe: 1.968 ± 0.39
9.721LeuGly: 9.721 ± 0.853
1.491LeuHis: 1.491 ± 0.239
2.147LeuIle: 2.147 ± 0.317
2.087LeuLys: 2.087 ± 0.597
7.276LeuLeu: 7.276 ± 0.725
1.014LeuMet: 1.014 ± 0.217
2.028LeuAsn: 2.028 ± 0.33
5.487LeuPro: 5.487 ± 0.565
1.491LeuGln: 1.491 ± 0.327
6.143LeuArg: 6.143 ± 0.595
2.982LeuSer: 2.982 ± 0.403
6.322LeuThr: 6.322 ± 0.61
5.845LeuVal: 5.845 ± 0.647
1.551LeuTrp: 1.551 ± 0.336
1.133LeuTyr: 1.133 ± 0.233
0.0LeuXaa: 0.0 ± 0.0
Met
2.386MetAla: 2.386 ± 0.317
0.119MetCys: 0.119 ± 0.091
0.537MetAsp: 0.537 ± 0.15
0.358MetGlu: 0.358 ± 0.113
0.835MetPhe: 0.835 ± 0.218
1.789MetGly: 1.789 ± 0.326
0.477MetHis: 0.477 ± 0.178
0.895MetIle: 0.895 ± 0.228
0.417MetLys: 0.417 ± 0.156
1.491MetLeu: 1.491 ± 0.262
0.358MetMet: 0.358 ± 0.149
0.656MetAsn: 0.656 ± 0.154
1.312MetPro: 1.312 ± 0.248
0.716MetGln: 0.716 ± 0.188
2.087MetArg: 2.087 ± 0.37
1.789MetSer: 1.789 ± 0.338
1.909MetThr: 1.909 ± 0.302
1.312MetVal: 1.312 ± 0.254
0.537MetTrp: 0.537 ± 0.146
0.06MetTyr: 0.06 ± 0.057
0.0MetXaa: 0.0 ± 0.0
Asn
3.638AsnAla: 3.638 ± 0.356
0.119AsnCys: 0.119 ± 0.118
0.477AsnAsp: 0.477 ± 0.16
0.895AsnGlu: 0.895 ± 0.213
0.417AsnPhe: 0.417 ± 0.175
2.386AsnGly: 2.386 ± 0.44
0.417AsnHis: 0.417 ± 0.162
0.895AsnIle: 0.895 ± 0.229
0.835AsnLys: 0.835 ± 0.199
2.028AsnLeu: 2.028 ± 0.399
0.179AsnMet: 0.179 ± 0.096
0.596AsnAsn: 0.596 ± 0.207
2.803AsnPro: 2.803 ± 0.47
0.477AsnGln: 0.477 ± 0.155
1.968AsnArg: 1.968 ± 0.412
1.789AsnSer: 1.789 ± 0.325
1.312AsnThr: 1.312 ± 0.314
1.61AsnVal: 1.61 ± 0.329
0.417AsnTrp: 0.417 ± 0.138
0.537AsnTyr: 0.537 ± 0.163
0.0AsnXaa: 0.0 ± 0.0
Pro
9.125ProAla: 9.125 ± 0.899
0.537ProCys: 0.537 ± 0.18
4.771ProAsp: 4.771 ± 0.401
4.712ProGlu: 4.712 ± 0.552
1.849ProPhe: 1.849 ± 0.369
7.515ProGly: 7.515 ± 0.781
1.252ProHis: 1.252 ± 0.281
2.624ProIle: 2.624 ± 0.388
1.014ProLys: 1.014 ± 0.261
3.996ProLeu: 3.996 ± 0.537
1.849ProMet: 1.849 ± 0.41
1.789ProAsn: 1.789 ± 0.333
7.336ProPro: 7.336 ± 0.971
1.849ProGln: 1.849 ± 0.292
5.427ProArg: 5.427 ± 0.884
3.459ProSer: 3.459 ± 0.494
4.652ProThr: 4.652 ± 0.483
5.845ProVal: 5.845 ± 0.56
1.312ProTrp: 1.312 ± 0.267
0.716ProTyr: 0.716 ± 0.202
0.0ProXaa: 0.0 ± 0.0
Gln
3.936GlnAla: 3.936 ± 0.537
0.477GlnCys: 0.477 ± 0.163
1.372GlnAsp: 1.372 ± 0.275
1.61GlnGlu: 1.61 ± 0.363
0.954GlnPhe: 0.954 ± 0.214
2.266GlnGly: 2.266 ± 0.482
0.656GlnHis: 0.656 ± 0.223
1.312GlnIle: 1.312 ± 0.252
0.775GlnLys: 0.775 ± 0.165
2.743GlnLeu: 2.743 ± 0.467
0.537GlnMet: 0.537 ± 0.161
0.656GlnAsn: 0.656 ± 0.203
1.431GlnPro: 1.431 ± 0.288
1.372GlnGln: 1.372 ± 0.286
2.922GlnArg: 2.922 ± 0.402
1.193GlnSer: 1.193 ± 0.358
1.312GlnThr: 1.312 ± 0.304
1.968GlnVal: 1.968 ± 0.376
0.775GlnTrp: 0.775 ± 0.193
0.477GlnTyr: 0.477 ± 0.178
0.0GlnXaa: 0.0 ± 0.0
Arg
8.648ArgAla: 8.648 ± 0.823
1.312ArgCys: 1.312 ± 0.36
3.757ArgAsp: 3.757 ± 0.422
5.248ArgGlu: 5.248 ± 0.603
2.445ArgPhe: 2.445 ± 0.422
7.157ArgGly: 7.157 ± 0.739
2.087ArgHis: 2.087 ± 0.401
3.877ArgIle: 3.877 ± 0.41
2.386ArgLys: 2.386 ± 0.381
6.441ArgLeu: 6.441 ± 0.732
2.087ArgMet: 2.087 ± 0.419
1.968ArgAsn: 1.968 ± 0.276
5.248ArgPro: 5.248 ± 0.647
2.743ArgGln: 2.743 ± 0.341
7.992ArgArg: 7.992 ± 0.908
2.982ArgSer: 2.982 ± 0.437
4.533ArgThr: 4.533 ± 0.637
5.666ArgVal: 5.666 ± 0.579
2.087ArgTrp: 2.087 ± 0.355
1.849ArgTyr: 1.849 ± 0.358
0.0ArgXaa: 0.0 ± 0.0
Ser
5.845SerAla: 5.845 ± 0.597
0.358SerCys: 0.358 ± 0.173
1.968SerAsp: 1.968 ± 0.357
1.73SerGlu: 1.73 ± 0.359
1.193SerPhe: 1.193 ± 0.243
4.533SerGly: 4.533 ± 0.663
1.074SerHis: 1.074 ± 0.212
2.266SerIle: 2.266 ± 0.383
1.431SerLys: 1.431 ± 0.255
3.042SerLeu: 3.042 ± 0.395
1.372SerMet: 1.372 ± 0.319
1.074SerAsn: 1.074 ± 0.222
3.459SerPro: 3.459 ± 0.425
1.014SerGln: 1.014 ± 0.256
2.743SerArg: 2.743 ± 0.416
1.73SerSer: 1.73 ± 0.329
3.459SerThr: 3.459 ± 0.494
2.684SerVal: 2.684 ± 0.341
0.895SerTrp: 0.895 ± 0.252
1.014SerTyr: 1.014 ± 0.29
0.0SerXaa: 0.0 ± 0.0
Thr
7.276ThrAla: 7.276 ± 0.895
0.835ThrCys: 0.835 ± 0.232
4.473ThrAsp: 4.473 ± 0.524
4.354ThrGlu: 4.354 ± 0.477
1.67ThrPhe: 1.67 ± 0.346
6.203ThrGly: 6.203 ± 0.675
1.491ThrHis: 1.491 ± 0.26
3.28ThrIle: 3.28 ± 0.516
1.193ThrLys: 1.193 ± 0.222
4.891ThrLeu: 4.891 ± 0.592
1.67ThrMet: 1.67 ± 0.317
0.954ThrAsn: 0.954 ± 0.267
4.95ThrPro: 4.95 ± 0.635
1.61ThrGln: 1.61 ± 0.364
4.771ThrArg: 4.771 ± 0.529
2.505ThrSer: 2.505 ± 0.382
4.056ThrThr: 4.056 ± 0.532
5.189ThrVal: 5.189 ± 0.636
1.252ThrTrp: 1.252 ± 0.256
1.968ThrTyr: 1.968 ± 0.369
0.0ThrXaa: 0.0 ± 0.0
Val
10.02ValAla: 10.02 ± 0.947
0.835ValCys: 0.835 ± 0.214
5.368ValAsp: 5.368 ± 0.657
5.368ValGlu: 5.368 ± 0.537
2.087ValPhe: 2.087 ± 0.369
6.799ValGly: 6.799 ± 0.744
1.252ValHis: 1.252 ± 0.327
2.982ValIle: 2.982 ± 0.457
1.491ValLys: 1.491 ± 0.363
5.427ValLeu: 5.427 ± 0.474
1.312ValMet: 1.312 ± 0.255
1.491ValAsn: 1.491 ± 0.319
4.891ValPro: 4.891 ± 0.503
1.789ValGln: 1.789 ± 0.333
5.427ValArg: 5.427 ± 0.612
3.698ValSer: 3.698 ± 0.49
4.95ValThr: 4.95 ± 0.621
5.904ValVal: 5.904 ± 0.755
1.431ValTrp: 1.431 ± 0.288
1.67ValTyr: 1.67 ± 0.301
0.0ValXaa: 0.0 ± 0.0
Trp
2.266TrpAla: 2.266 ± 0.353
0.298TrpCys: 0.298 ± 0.137
0.656TrpAsp: 0.656 ± 0.189
0.775TrpGlu: 0.775 ± 0.162
0.835TrpPhe: 0.835 ± 0.2
1.014TrpGly: 1.014 ± 0.222
0.895TrpHis: 0.895 ± 0.273
0.775TrpIle: 0.775 ± 0.213
0.298TrpLys: 0.298 ± 0.118
2.207TrpLeu: 2.207 ± 0.395
0.417TrpMet: 0.417 ± 0.148
0.358TrpAsn: 0.358 ± 0.171
1.551TrpPro: 1.551 ± 0.377
0.716TrpGln: 0.716 ± 0.167
1.67TrpArg: 1.67 ± 0.318
1.014TrpSer: 1.014 ± 0.219
1.014TrpThr: 1.014 ± 0.221
0.954TrpVal: 0.954 ± 0.233
0.298TrpTrp: 0.298 ± 0.134
0.417TrpTyr: 0.417 ± 0.14
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.551TyrAla: 1.551 ± 0.243
0.298TyrCys: 0.298 ± 0.121
1.074TyrAsp: 1.074 ± 0.241
0.477TyrGlu: 0.477 ± 0.174
0.596TyrPhe: 0.596 ± 0.193
1.909TyrGly: 1.909 ± 0.407
0.716TyrHis: 0.716 ± 0.218
0.477TyrIle: 0.477 ± 0.191
0.119TyrLys: 0.119 ± 0.085
1.61TyrLeu: 1.61 ± 0.342
0.417TyrMet: 0.417 ± 0.155
0.358TyrAsn: 0.358 ± 0.123
1.133TyrPro: 1.133 ± 0.215
0.954TyrGln: 0.954 ± 0.226
2.266TyrArg: 2.266 ± 0.272
1.431TyrSer: 1.431 ± 0.286
2.028TyrThr: 2.028 ± 0.45
1.67TyrVal: 1.67 ± 0.266
0.417TyrTrp: 0.417 ± 0.146
0.358TyrTyr: 0.358 ± 0.155
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 89 proteins (16768 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski