Human stool-associated circular virus NG13

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Circovirus; Human associated circovirus 1

Average proteome isoelectric point is 8.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4N3R1|D4N3R1_9CIRC Capsid protein OS=Human stool-associated circular virus NG13 OX=743300 GN=cap PE=3 SV=1
MM1 pKa = 7.19EE2 pKa = 5.07SKK4 pKa = 10.33RR5 pKa = 11.84KK6 pKa = 9.61KK7 pKa = 9.23RR8 pKa = 11.84QNPAKK13 pKa = 9.8RR14 pKa = 11.84WCFTLNNYY22 pKa = 7.21TQLEE26 pKa = 4.77AITIEE31 pKa = 3.93QLLCTEE37 pKa = 4.63EE38 pKa = 3.72VQYY41 pKa = 11.21AIVGEE46 pKa = 4.26EE47 pKa = 3.66IGEE50 pKa = 4.21NGTPHH55 pKa = 6.43LQGFFNLKK63 pKa = 9.11KK64 pKa = 10.33KK65 pKa = 10.43KK66 pKa = 10.26RR67 pKa = 11.84LTSLKK72 pKa = 10.4AWLNDD77 pKa = 3.29RR78 pKa = 11.84AHH80 pKa = 6.62YY81 pKa = 10.47EE82 pKa = 3.92EE83 pKa = 5.58AKK85 pKa = 10.97GSDD88 pKa = 3.42EE89 pKa = 4.06QNRR92 pKa = 11.84RR93 pKa = 11.84YY94 pKa = 9.75CSKK97 pKa = 10.48SGNILISFGSPQKK110 pKa = 10.37QGQRR114 pKa = 11.84NDD116 pKa = 3.29LVLAAEE122 pKa = 4.45LLKK125 pKa = 11.06EE126 pKa = 4.29SGGNMAAVADD136 pKa = 5.04LYY138 pKa = 10.11PSAVIRR144 pKa = 11.84YY145 pKa = 8.43GRR147 pKa = 11.84GLQQYY152 pKa = 7.26WQLIGFSARR161 pKa = 11.84DD162 pKa = 3.45FKK164 pKa = 11.28TEE166 pKa = 3.54VFVYY170 pKa = 9.98VGPPGCGKK178 pKa = 10.19SRR180 pKa = 11.84AAAEE184 pKa = 4.23LGAASGGKK192 pKa = 9.5VYY194 pKa = 10.59YY195 pKa = 10.04KK196 pKa = 10.51PRR198 pKa = 11.84GEE200 pKa = 3.68WWDD203 pKa = 3.9GYY205 pKa = 11.0NGEE208 pKa = 4.32ATVIIDD214 pKa = 5.8DD215 pKa = 4.44FYY217 pKa = 11.89GWLKK221 pKa = 10.65YY222 pKa = 10.66DD223 pKa = 3.65EE224 pKa = 5.04LLRR227 pKa = 11.84LCDD230 pKa = 4.23RR231 pKa = 11.84YY232 pKa = 9.64PHH234 pKa = 6.38RR235 pKa = 11.84VPVKK239 pKa = 9.11GGFVQFCSKK248 pKa = 10.7RR249 pKa = 11.84IILTSNIHH257 pKa = 3.1VWMWYY262 pKa = 10.03RR263 pKa = 11.84FEE265 pKa = 6.21SYY267 pKa = 10.31DD268 pKa = 3.15ASALMRR274 pKa = 11.84RR275 pKa = 11.84INVYY279 pKa = 10.23KK280 pKa = 10.24LWNGSVCTFDD290 pKa = 5.07DD291 pKa = 4.88LCDD294 pKa = 3.43EE295 pKa = 5.06KK296 pKa = 11.75YY297 pKa = 11.38NFLTPLKK304 pKa = 10.35YY305 pKa = 10.68NYY307 pKa = 10.74

Molecular weight: 35.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4N3R1|D4N3R1_9CIRC Capsid protein OS=Human stool-associated circular virus NG13 OX=743300 GN=cap PE=3 SV=1
MM1 pKa = 7.55GGRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84QRR8 pKa = 11.84RR9 pKa = 11.84PDD11 pKa = 3.13PRR13 pKa = 11.84RR14 pKa = 11.84GRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84ARR20 pKa = 11.84TFLPARR26 pKa = 11.84LYY28 pKa = 9.98GQTYY32 pKa = 6.31STRR35 pKa = 11.84LIKK38 pKa = 10.22QDD40 pKa = 3.39TFRR43 pKa = 11.84VDD45 pKa = 3.0AAGKK49 pKa = 10.16GVGLTFTLGEE59 pKa = 4.31FLTGQITWDD68 pKa = 3.51YY69 pKa = 10.7YY70 pKa = 10.57RR71 pKa = 11.84INTIVVTFLPQINPTMPIDD90 pKa = 3.34QGTGGGGFNEE100 pKa = 4.42TSVDD104 pKa = 4.24FDD106 pKa = 4.42DD107 pKa = 4.64ATPPTSKK114 pKa = 10.17ISMEE118 pKa = 3.66NWNNTKK124 pKa = 9.76FWRR127 pKa = 11.84NDD129 pKa = 2.83RR130 pKa = 11.84KK131 pKa = 9.29FTIKK135 pKa = 10.15FRR137 pKa = 11.84PVFHH141 pKa = 7.0RR142 pKa = 11.84LVATSATSTTPVSAEE157 pKa = 3.46FNQRR161 pKa = 11.84RR162 pKa = 11.84NRR164 pKa = 11.84GVWLNSAYY172 pKa = 9.91KK173 pKa = 10.09DD174 pKa = 3.38IPHH177 pKa = 6.98FGLKK181 pKa = 9.29TFFVNNFTNPAQNTIIYY198 pKa = 9.63QILIKK203 pKa = 10.45AYY205 pKa = 9.99VSFKK209 pKa = 10.46RR210 pKa = 11.84PIWVGTTTEE219 pKa = 4.06NQQ221 pKa = 3.1

Molecular weight: 25.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

528

221

307

264.0

30.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.061 ± 0.689

1.515 ± 0.963

4.545 ± 0.013

4.735 ± 1.572

5.682 ± 1.278

8.144 ± 0.287

1.136 ± 0.147

5.303 ± 0.656

6.25 ± 1.097

7.386 ± 1.532

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.326 ± 0.02

5.871 ± 0.582

4.356 ± 0.97

4.356 ± 0.107

8.144 ± 1.439

4.924 ± 0.542

7.197 ± 3.192

5.303 ± 0.081

2.652 ± 0.247

5.114 ± 1.237
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski