Beihai tombus-like virus 15

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFF2|A0A1L3KFF2_9VIRU RNA-directed RNA polymerase OS=Beihai tombus-like virus 15 OX=1922718 PE=4 SV=1
MM1 pKa = 7.76ALNLKK6 pKa = 8.8TNSFVLDD13 pKa = 3.93TEE15 pKa = 4.23QEE17 pKa = 3.9RR18 pKa = 11.84ALARR22 pKa = 11.84WGFNLKK28 pKa = 10.37SGTIPHH34 pKa = 6.23THH36 pKa = 6.69PVSALIRR43 pKa = 11.84NGFEE47 pKa = 3.8DD48 pKa = 4.99AVINQHH54 pKa = 5.15KK55 pKa = 10.08DD56 pKa = 3.01KK57 pKa = 10.62RR58 pKa = 11.84IKK60 pKa = 10.73DD61 pKa = 3.41VGGNYY66 pKa = 9.26ARR68 pKa = 11.84HH69 pKa = 6.14AARR72 pKa = 11.84HH73 pKa = 5.05QGFDD77 pKa = 3.0IHH79 pKa = 6.8SCCPLLSPDD88 pKa = 3.64DD89 pKa = 4.69LIRR92 pKa = 11.84EE93 pKa = 4.44VTRR96 pKa = 11.84DD97 pKa = 3.43MAVDD101 pKa = 3.91PDD103 pKa = 3.92CCRR106 pKa = 11.84KK107 pKa = 9.92RR108 pKa = 11.84FQDD111 pKa = 3.48CSRR114 pKa = 11.84PADD117 pKa = 3.15VYY119 pKa = 10.37MFNHH123 pKa = 5.29SMYY126 pKa = 10.3YY127 pKa = 9.82VEE129 pKa = 5.84PEE131 pKa = 4.2DD132 pKa = 4.05LACIPVGACIYY143 pKa = 10.05SLHH146 pKa = 6.83HH147 pKa = 7.19LYY149 pKa = 10.29QGPGTYY155 pKa = 9.93CAGEE159 pKa = 4.07MEE161 pKa = 4.56VTQDD165 pKa = 3.22EE166 pKa = 4.56QGVYY170 pKa = 9.43TVDD173 pKa = 2.82TRR175 pKa = 11.84GNNRR179 pKa = 11.84PYY181 pKa = 10.71KK182 pKa = 10.38HH183 pKa = 5.79KK184 pKa = 10.63QCWLTGSCVIPGKK197 pKa = 10.35DD198 pKa = 3.09GAVIAFTVLRR208 pKa = 11.84NYY210 pKa = 10.32EE211 pKa = 4.04SAHH214 pKa = 4.6VFKK217 pKa = 10.95GYY219 pKa = 9.78VYY221 pKa = 10.44KK222 pKa = 11.06VEE224 pKa = 4.08DD225 pKa = 3.86SYY227 pKa = 12.12RR228 pKa = 11.84LLSQPVFKK236 pKa = 10.31PYY238 pKa = 10.4DD239 pKa = 3.47IYY241 pKa = 10.83PSEE244 pKa = 5.34LEE246 pKa = 5.16DD247 pKa = 3.41ILINEE252 pKa = 4.72GTSQTIDD259 pKa = 2.83NRR261 pKa = 11.84TLHH264 pKa = 6.09NMASRR269 pKa = 11.84ARR271 pKa = 11.84TYY273 pKa = 11.59ALTTHH278 pKa = 6.94RR279 pKa = 11.84KK280 pKa = 7.5MPANIADD287 pKa = 4.22IYY289 pKa = 11.5ASAANKK295 pKa = 9.66AAEE298 pKa = 4.39INIKK302 pKa = 7.82TQSQLNKK309 pKa = 10.44EE310 pKa = 4.33KK311 pKa = 10.64IEE313 pKa = 4.01TSNRR317 pKa = 11.84LIAGLEE323 pKa = 3.85IDD325 pKa = 4.03TSQPHH330 pKa = 5.03IPIYY334 pKa = 10.36RR335 pKa = 11.84RR336 pKa = 11.84VGGILWTLASGVVRR350 pKa = 11.84TTAYY354 pKa = 9.67TSLDD358 pKa = 3.61YY359 pKa = 10.69MKK361 pKa = 10.85AGALLPAKK369 pKa = 10.2AIIYY373 pKa = 5.08TTSRR377 pKa = 11.84IRR379 pKa = 11.84QCLTYY384 pKa = 10.43EE385 pKa = 4.03NNDD388 pKa = 3.51YY389 pKa = 11.02VVPTCTFVPLNSWEE403 pKa = 4.23NEE405 pKa = 3.83LTSILLRR412 pKa = 11.84AVPDD416 pKa = 3.96TVTTTIDD423 pKa = 3.4NKK425 pKa = 10.51IMEE428 pKa = 4.86SADD431 pKa = 3.61KK432 pKa = 10.33LAKK435 pKa = 10.55LIGYY439 pKa = 9.14VDD441 pKa = 3.76APMHH445 pKa = 5.78FDD447 pKa = 3.03EE448 pKa = 4.67WVEE451 pKa = 3.97RR452 pKa = 11.84YY453 pKa = 9.17PPARR457 pKa = 11.84QEE459 pKa = 4.3QLRR462 pKa = 11.84KK463 pKa = 9.78AHH465 pKa = 5.88YY466 pKa = 9.95KK467 pKa = 9.57PLNHH471 pKa = 6.3NVDD474 pKa = 3.39MFIKK478 pKa = 10.07IEE480 pKa = 3.85QLDD483 pKa = 4.06DD484 pKa = 3.65QKK486 pKa = 11.28HH487 pKa = 5.08PRR489 pKa = 11.84AIQARR494 pKa = 11.84HH495 pKa = 6.39DD496 pKa = 4.02SYY498 pKa = 11.41KK499 pKa = 10.9SKK501 pKa = 10.66LGPWVARR508 pKa = 11.84LEE510 pKa = 4.24KK511 pKa = 10.49RR512 pKa = 11.84CIEE515 pKa = 4.13ALPNFVKK522 pKa = 10.7KK523 pKa = 11.02LNDD526 pKa = 3.16EE527 pKa = 3.96QRR529 pKa = 11.84AEE531 pKa = 4.06KK532 pKa = 10.51VAEE535 pKa = 4.44LKK537 pKa = 10.87CKK539 pKa = 10.11ADD541 pKa = 3.99NIIEE545 pKa = 4.03IDD547 pKa = 3.46FTRR550 pKa = 11.84FDD552 pKa = 4.13RR553 pKa = 11.84NCTKK557 pKa = 10.44EE558 pKa = 4.06LLSATEE564 pKa = 4.04HH565 pKa = 6.07YY566 pKa = 10.37IYY568 pKa = 11.02DD569 pKa = 3.51KK570 pKa = 11.29VFPKK574 pKa = 10.53EE575 pKa = 3.86IASLLHH581 pKa = 5.5LQLRR585 pKa = 11.84NKK587 pKa = 9.08VRR589 pKa = 11.84SARR592 pKa = 11.84GYY594 pKa = 7.79TYY596 pKa = 10.54SVEE599 pKa = 4.15GTRR602 pKa = 11.84MSGDD606 pKa = 3.37VNTSIGNCLIVLCLSLAAGLEE627 pKa = 4.15LEE629 pKa = 4.73NILVEE634 pKa = 4.47GDD636 pKa = 3.78DD637 pKa = 4.46MIAAASDD644 pKa = 2.96ITLQKK649 pKa = 10.85FSDD652 pKa = 4.04EE653 pKa = 4.43VIKK656 pKa = 10.02ATGMIPKK663 pKa = 8.89TVVTHH668 pKa = 6.92RR669 pKa = 11.84GGSFCSRR676 pKa = 11.84YY677 pKa = 10.37DD678 pKa = 3.71VIDD681 pKa = 3.75SEE683 pKa = 4.67GKK685 pKa = 8.32PRR687 pKa = 11.84RR688 pKa = 11.84VRR690 pKa = 11.84HH691 pKa = 5.5PLRR694 pKa = 11.84DD695 pKa = 3.24ITRR698 pKa = 11.84YY699 pKa = 10.42GYY701 pKa = 8.0TLHH704 pKa = 7.73GEE706 pKa = 4.23DD707 pKa = 3.8RR708 pKa = 11.84LEE710 pKa = 3.98RR711 pKa = 11.84AQRR714 pKa = 11.84HH715 pKa = 4.14YY716 pKa = 10.74KK717 pKa = 9.41EE718 pKa = 4.36WVGVPMLGPVYY729 pKa = 10.59TNILKK734 pKa = 9.91QLQPDD739 pKa = 3.74VQIPEE744 pKa = 3.93IEE746 pKa = 3.97ITAEE750 pKa = 3.75ARR752 pKa = 11.84VSFARR757 pKa = 11.84TFDD760 pKa = 3.42ISIKK764 pKa = 7.47TQQLFEE770 pKa = 4.31EE771 pKa = 5.16DD772 pKa = 2.93KK773 pKa = 10.43SQRR776 pKa = 11.84ARR778 pKa = 11.84IYY780 pKa = 11.33NDD782 pKa = 2.96LTQEE786 pKa = 4.42KK787 pKa = 9.41IYY789 pKa = 9.48KK790 pKa = 8.36TALTEE795 pKa = 4.06AVEE798 pKa = 4.39TPIIGPGDD806 pKa = 3.91SVSQISGTPGSSEE819 pKa = 3.97TGHH822 pKa = 7.4DD823 pKa = 3.64KK824 pKa = 11.23LQVPTWNIRR833 pKa = 11.84TGSLGSTSKK842 pKa = 10.67NVRR845 pKa = 11.84DD846 pKa = 4.02VQVKK850 pKa = 9.91GPSEE854 pKa = 4.24SGLQNSLRR862 pKa = 11.84DD863 pKa = 3.69DD864 pKa = 3.74NKK866 pKa = 10.64RR867 pKa = 11.84GNTHH871 pKa = 6.42RR872 pKa = 11.84NRR874 pKa = 11.84LRR876 pKa = 11.84RR877 pKa = 11.84HH878 pKa = 5.4RR879 pKa = 11.84RR880 pKa = 11.84QARR883 pKa = 11.84LHH885 pKa = 5.1GHH887 pKa = 6.0GSIVSKK893 pKa = 11.39DD894 pKa = 3.58NVTGLAGLPGGSSKK908 pKa = 10.81RR909 pKa = 11.84SGNEE913 pKa = 3.06TEE915 pKa = 4.58MAMHH919 pKa = 7.47CDD921 pKa = 3.32PSKK924 pKa = 10.18PNSSIGQYY932 pKa = 9.59SIRR935 pKa = 11.84NPSTT939 pKa = 3.01

Molecular weight: 105.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFF2|A0A1L3KFF2_9VIRU RNA-directed RNA polymerase OS=Beihai tombus-like virus 15 OX=1922718 PE=4 SV=1
MM1 pKa = 7.34YY2 pKa = 10.0KK3 pKa = 9.8YY4 pKa = 10.2QKK6 pKa = 10.69SKK8 pKa = 10.95LPQKK12 pKa = 10.23LAYY15 pKa = 9.11PSQEE19 pKa = 3.8HH20 pKa = 5.76STSQSKK26 pKa = 10.55HH27 pKa = 5.6SNSSKK32 pKa = 10.38KK33 pKa = 10.12ISPKK37 pKa = 9.33EE38 pKa = 3.87HH39 pKa = 6.34VSTMTSPKK47 pKa = 9.92RR48 pKa = 11.84KK49 pKa = 7.26STKK52 pKa = 9.54RR53 pKa = 11.84PSPKK57 pKa = 9.08RR58 pKa = 11.84SKK60 pKa = 10.62RR61 pKa = 11.84PSLAPGTLLVKK72 pKa = 10.48YY73 pKa = 9.91RR74 pKa = 11.84EE75 pKa = 3.96LLAPVKK81 pKa = 10.55QGTTSYY87 pKa = 10.76KK88 pKa = 10.01FRR90 pKa = 11.84PGTSGLAHH98 pKa = 7.2LDD100 pKa = 3.13QRR102 pKa = 11.84ARR104 pKa = 11.84MYY106 pKa = 11.04EE107 pKa = 4.06MYY109 pKa = 10.5RR110 pKa = 11.84LRR112 pKa = 11.84GPVRR116 pKa = 11.84VGYY119 pKa = 8.25KK120 pKa = 8.57TASGTTTNGEE130 pKa = 3.88ILIGIDD136 pKa = 4.86FDD138 pKa = 3.97ATDD141 pKa = 4.41DD142 pKa = 3.79RR143 pKa = 11.84LDD145 pKa = 3.8YY146 pKa = 10.54MGTAALSPKK155 pKa = 9.52TMSPVWRR162 pKa = 11.84DD163 pKa = 3.47CQVVVPKK170 pKa = 10.63DD171 pKa = 3.08RR172 pKa = 11.84AMKK175 pKa = 10.09QKK177 pKa = 9.25WLCTATRR184 pKa = 11.84ASQTVASDD192 pKa = 3.24NTAFAIQVHH201 pKa = 5.69NSGPTGCGSLWVEE214 pKa = 4.3YY215 pKa = 9.77YY216 pKa = 11.08LEE218 pKa = 4.4FMSPCVNSEE227 pKa = 4.23DD228 pKa = 4.05VGIAYY233 pKa = 7.56ITTSGNGGGHH243 pKa = 6.46GGSVVGNPGLVPGGHH258 pKa = 6.11SKK260 pKa = 11.04AFAIPDD266 pKa = 3.46ALMPAFNEE274 pKa = 4.07ACKK277 pKa = 10.52AFPDD281 pKa = 3.65VMKK284 pKa = 10.31TVLVPDD290 pKa = 4.85GAPGGHH296 pKa = 6.24SYY298 pKa = 11.22INLEE302 pKa = 3.85NKK304 pKa = 9.83GYY306 pKa = 10.45KK307 pKa = 9.41DD308 pKa = 3.93AYY310 pKa = 9.89VSLPSTHH317 pKa = 6.56WPSSQQISFWFAAPSVAILVRR338 pKa = 11.84YY339 pKa = 8.95LLNKK343 pKa = 9.25NHH345 pKa = 6.63SSVV348 pKa = 3.16

Molecular weight: 37.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1287

348

939

643.5

71.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.148 ± 0.291

1.865 ± 0.204

5.594 ± 0.749

5.128 ± 1.074

2.331 ± 0.122

6.527 ± 0.724

3.186 ± 0.149

5.828 ± 1.27

6.371 ± 0.661

7.615 ± 0.342

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.098 ± 0.37

4.429 ± 0.467

5.517 ± 0.931

3.73 ± 0.134

6.449 ± 0.882

7.848 ± 1.463

6.76 ± 0.065

6.294 ± 0.287

1.01 ± 0.203

4.274 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski