Mycobacterium phage Ruotula

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1CJ84|A0A2P1CJ84_9CAUD Recombination directionality factor OS=Mycobacterium phage Ruotula OX=2099448 GN=54 PE=4 SV=1
MM1 pKa = 7.35PQTVHH6 pKa = 6.12VLPVDD11 pKa = 3.73DD12 pKa = 5.86LIEE15 pKa = 6.01HH16 pKa = 7.01EE17 pKa = 5.59DD18 pKa = 3.6VGDD21 pKa = 4.07DD22 pKa = 3.95CVCGPEE28 pKa = 4.04IEE30 pKa = 4.64PVFDD34 pKa = 5.12ADD36 pKa = 4.79GACGWVITHH45 pKa = 6.86HH46 pKa = 6.49SLDD49 pKa = 3.22GRR51 pKa = 11.84EE52 pKa = 3.86RR53 pKa = 11.84FEE55 pKa = 3.97

Molecular weight: 6.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1CJC8|A0A2P1CJC8_9CAUD Uncharacterized protein OS=Mycobacterium phage Ruotula OX=2099448 GN=51 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 8.68PTARR6 pKa = 11.84PNLLRR11 pKa = 11.84QQILSEE17 pKa = 4.03LLANRR22 pKa = 11.84TVTRR26 pKa = 11.84TEE28 pKa = 3.96TQPKK32 pKa = 9.03KK33 pKa = 10.61GPKK36 pKa = 9.66ASVIHH41 pKa = 6.44PVTGEE46 pKa = 3.66RR47 pKa = 11.84VTYY50 pKa = 7.68QTYY53 pKa = 10.38VDD55 pKa = 4.27APVDD59 pKa = 3.73RR60 pKa = 11.84QVPGLAGHH68 pKa = 5.95VSEE71 pKa = 5.18EE72 pKa = 4.24NIDD75 pKa = 3.46RR76 pKa = 11.84LARR79 pKa = 11.84RR80 pKa = 11.84WGRR83 pKa = 3.28

Molecular weight: 9.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

16589

28

944

176.5

19.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.693 ± 0.426

0.711 ± 0.123

6.396 ± 0.282

6.498 ± 0.31

3.267 ± 0.157

8.861 ± 0.537

2.062 ± 0.169

4.913 ± 0.203

4.105 ± 0.22

8.433 ± 0.308

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.086 ± 0.115

3.201 ± 0.133

5.618 ± 0.256

3.328 ± 0.222

6.221 ± 0.38

5.889 ± 0.199

6.336 ± 0.256

7.276 ± 0.223

2.092 ± 0.144

3.014 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski