Dictyostelium discoideum (Slime mold)

Taxonomy: cellular organisms; Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales; Dictyosteliaceae; Dictyostelium

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12755 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q54US9|Q54US9_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0218266 PE=4 SV=1
MM1 pKa = 8.5IMFNLDD7 pKa = 3.43DD8 pKa = 4.07QEE10 pKa = 5.59CPDD13 pKa = 3.69LEE15 pKa = 4.38EE16 pKa = 4.43NNFYY20 pKa = 11.09NEE22 pKa = 4.03QQCPDD27 pKa = 3.43INHH30 pKa = 6.94KK31 pKa = 10.45KK32 pKa = 10.64GSNQRR37 pKa = 11.84SINYY41 pKa = 9.39NGNNVNRR48 pKa = 11.84NSMSHH53 pKa = 6.8RR54 pKa = 11.84YY55 pKa = 8.75EE56 pKa = 4.0PYY58 pKa = 8.87KK59 pKa = 9.46TRR61 pKa = 11.84NYY63 pKa = 10.74NNQNEE68 pKa = 4.57NINNFNIINSGNANEE83 pKa = 4.8DD84 pKa = 4.61DD85 pKa = 4.25EE86 pKa = 7.15DD87 pKa = 4.8EE88 pKa = 6.15DD89 pKa = 6.22DD90 pKa = 6.68DD91 pKa = 7.05SDD93 pKa = 6.57DD94 pKa = 6.24DD95 pKa = 6.74DD96 pKa = 6.71DD97 pKa = 6.55DD98 pKa = 4.45NSNGNTYY105 pKa = 11.12NFVSDD110 pKa = 4.23YY111 pKa = 8.79NQQYY115 pKa = 11.51AFIQYY120 pKa = 9.51VNRR123 pKa = 11.84QFHH126 pKa = 6.11LRR128 pKa = 11.84NYY130 pKa = 9.39RR131 pKa = 11.84QVNMQEE137 pKa = 4.06SQNLIEE143 pKa = 5.93QILQTYY149 pKa = 9.23KK150 pKa = 10.77NQSQSSDD157 pKa = 3.38DD158 pKa = 3.76NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNDD206 pKa = 3.61RR207 pKa = 11.84PDD209 pKa = 3.5NDD211 pKa = 4.04EE212 pKa = 4.89NLQNDD217 pKa = 3.54QNQEE221 pKa = 3.87QNEE224 pKa = 4.29EE225 pKa = 3.83EE226 pKa = 4.31DD227 pKa = 3.83EE228 pKa = 4.31EE229 pKa = 4.7EE230 pKa = 4.3EE231 pKa = 5.46EE232 pKa = 3.94EE233 pKa = 4.15DD234 pKa = 3.46QYY236 pKa = 12.14EE237 pKa = 4.19EE238 pKa = 5.4GEE240 pKa = 4.42EE241 pKa = 4.14EE242 pKa = 4.24DD243 pKa = 3.26QSEE246 pKa = 4.37YY247 pKa = 10.85QEE249 pKa = 4.06QQSQNLFQQDD259 pKa = 2.76QDD261 pKa = 4.21FEE263 pKa = 4.35QLQQDD268 pKa = 3.39QDD270 pKa = 3.77LSLQSSHH277 pKa = 6.83IFFF280 pKa = 5.41

Molecular weight: 33.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q7KWW3|Q7KWW3_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0276789 PE=4 SV=1
MM1 pKa = 7.65ARR3 pKa = 11.84EE4 pKa = 3.93LWLTHH9 pKa = 5.98PRR11 pKa = 11.84NFGPGSRR18 pKa = 11.84TCRR21 pKa = 11.84KK22 pKa = 9.6CGNHH26 pKa = 6.19HH27 pKa = 6.78GIIRR31 pKa = 11.84KK32 pKa = 9.31YY33 pKa = 10.01DD34 pKa = 3.51LNMCRR39 pKa = 11.84RR40 pKa = 11.84CFRR43 pKa = 11.84TDD45 pKa = 2.78AEE47 pKa = 4.49AIGFNKK53 pKa = 9.55YY54 pKa = 7.81RR55 pKa = 3.79

Molecular weight: 6.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12728

27

12755

6860848

12

11103

537.9

61.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.031 ± 0.022

1.423 ± 0.012

5.201 ± 0.018

5.826 ± 0.026

4.706 ± 0.021

4.445 ± 0.02

1.76 ± 0.01

8.437 ± 0.027

7.662 ± 0.026

8.571 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.563 ± 0.008

11.36 ± 0.067

3.956 ± 0.018

5.071 ± 0.041

2.789 ± 0.016

9.7 ± 0.032

5.984 ± 0.023

4.244 ± 0.018

0.725 ± 0.006

3.544 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski