Megasphaera micronuciformis F0359

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Veillonellales; Veillonellaceae; Megasphaera; Megasphaera micronuciformis

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1771 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E2ZBB6|E2ZBB6_9FIRM DNA-directed RNA polymerase subunit beta' OS=Megasphaera micronuciformis F0359 OX=706434 GN=rpoC PE=3 SV=1
MM1 pKa = 7.54ANVSLGMTGTRR12 pKa = 11.84VASVQNMLIGTGYY25 pKa = 8.81LTDD28 pKa = 3.93GADD31 pKa = 3.48GVFGSGTQAAVQRR44 pKa = 11.84FQADD48 pKa = 2.92HH49 pKa = 6.88GLTADD54 pKa = 5.11GIVGTQTMNALSTASGQPIPVDD76 pKa = 3.12NTIVMEE82 pKa = 4.36ATAYY86 pKa = 8.9TADD89 pKa = 3.79DD90 pKa = 4.88PGNSGYY96 pKa = 8.42TATGQPLAYY105 pKa = 10.2GMVSVDD111 pKa = 3.65PNVIPLGSQLYY122 pKa = 9.95IEE124 pKa = 5.25GYY126 pKa = 9.69GYY128 pKa = 11.17AVAADD133 pKa = 3.65TGGGIVGNRR142 pKa = 11.84IDD144 pKa = 4.14LAMDD148 pKa = 3.87SVSDD152 pKa = 3.57ALNFGRR158 pKa = 11.84RR159 pKa = 11.84DD160 pKa = 3.65VVVHH164 pKa = 5.74VLL166 pKa = 3.34

Molecular weight: 16.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E2Z9N1|E2Z9N1_9FIRM Serine acetyltransferase OS=Megasphaera micronuciformis F0359 OX=706434 GN=cysE PE=3 SV=1
MM1 pKa = 7.29KK2 pKa = 9.51QTFQPNNLWKK12 pKa = 10.57KK13 pKa = 7.58RR14 pKa = 11.84THH16 pKa = 6.26GFRR19 pKa = 11.84EE20 pKa = 3.79RR21 pKa = 11.84MKK23 pKa = 9.87TKK25 pKa = 10.01GGRR28 pKa = 11.84MVLKK32 pKa = 9.89KK33 pKa = 10.47RR34 pKa = 11.84RR35 pKa = 11.84MKK37 pKa = 10.56GRR39 pKa = 11.84KK40 pKa = 8.82KK41 pKa = 10.62LSAA44 pKa = 3.95

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1771

0

1771

534718

37

2811

301.9

33.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.715 ± 0.07

1.245 ± 0.023

5.744 ± 0.048

6.72 ± 0.068

3.913 ± 0.051

7.663 ± 0.064

2.063 ± 0.028

6.665 ± 0.052

6.235 ± 0.046

9.059 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.79 ± 0.031

3.733 ± 0.053

3.658 ± 0.037

3.0 ± 0.031

5.094 ± 0.057

5.503 ± 0.048

5.933 ± 0.057

7.733 ± 0.045

0.867 ± 0.022

3.665 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski