Botryobasidium botryosum FD-172 SS1

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Cantharellales; Botryobasidiaceae; Botryobasidium; Botryobasidium botryosum

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16484 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A067MEI8|A0A067MEI8_9AGAM Uncharacterized protein OS=Botryobasidium botryosum FD-172 SS1 OX=930990 GN=BOTBODRAFT_47140 PE=4 SV=1
MM1 pKa = 7.12STTPRR6 pKa = 11.84LDD8 pKa = 3.62DD9 pKa = 3.44QTDD12 pKa = 3.79GTEE15 pKa = 4.12SDD17 pKa = 4.34HH18 pKa = 6.71MPDD21 pKa = 3.66LAPYY25 pKa = 10.71DD26 pKa = 4.07SDD28 pKa = 5.01DD29 pKa = 4.74DD30 pKa = 5.63DD31 pKa = 4.46MVLSDD36 pKa = 3.99SPPRR40 pKa = 11.84EE41 pKa = 4.37TVPSTPGEE49 pKa = 4.07GDD51 pKa = 3.37APFYY55 pKa = 11.25LCGDD59 pKa = 3.53ANFRR63 pKa = 11.84LRR65 pKa = 11.84DD66 pKa = 3.33GAAVVLSYY74 pKa = 11.15DD75 pKa = 3.97VNCQMFPKK83 pKa = 9.32LHH85 pKa = 5.64QMVHH89 pKa = 4.68QHH91 pKa = 6.1HH92 pKa = 7.29CDD94 pKa = 2.98AASIHH99 pKa = 6.52GSDD102 pKa = 5.47DD103 pKa = 3.66DD104 pKa = 5.31DD105 pKa = 4.88SPRR108 pKa = 11.84DD109 pKa = 3.8PLPSDD114 pKa = 4.67GPHH117 pKa = 5.78QDD119 pKa = 3.1EE120 pKa = 4.57AAGPLEE126 pKa = 5.38GEE128 pKa = 4.37DD129 pKa = 4.18LDD131 pKa = 4.36YY132 pKa = 11.58SADD135 pKa = 3.8AEE137 pKa = 4.21NDD139 pKa = 3.63GAGQHH144 pKa = 6.1VDD146 pKa = 3.13QQ147 pKa = 5.47

Molecular weight: 15.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A067MS39|A0A067MS39_9AGAM Uncharacterized protein OS=Botryobasidium botryosum FD-172 SS1 OX=930990 GN=BOTBODRAFT_55512 PE=4 SV=1
MM1 pKa = 7.34LQPTIAHH8 pKa = 7.71RR9 pKa = 11.84ITQPLKK15 pKa = 10.03GSRR18 pKa = 11.84PGHH21 pKa = 4.77TAARR25 pKa = 11.84RR26 pKa = 11.84QQTRR30 pKa = 11.84VGSVWPHH37 pKa = 6.13PSSTQRR43 pKa = 11.84QPGQQRR49 pKa = 11.84STPRR53 pKa = 3.1

Molecular weight: 5.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16483

1

16484

6416465

34

7295

389.3

42.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.253 ± 0.018

1.402 ± 0.008

5.354 ± 0.011

5.701 ± 0.016

3.709 ± 0.012

6.541 ± 0.018

2.704 ± 0.008

4.872 ± 0.014

4.312 ± 0.016

9.458 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.985 ± 0.007

3.185 ± 0.011

6.94 ± 0.022

3.508 ± 0.013

6.411 ± 0.017

8.61 ± 0.021

5.881 ± 0.012

6.138 ± 0.015

1.449 ± 0.006

2.584 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski