Rhizobacter sp. Root29

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Rhizobacter; unclassified Rhizobacter

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5611 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q6MSU9|A0A0Q6MSU9_9BURK Chromosomal replication initiator protein DnaA OS=Rhizobacter sp. Root29 OX=1736511 GN=dnaA PE=3 SV=1
MM1 pKa = 7.41NNKK4 pKa = 9.22LLHH7 pKa = 6.46AFGIALTGAALLTACGGGGGSGGDD31 pKa = 3.59APAPDD36 pKa = 4.34DD37 pKa = 3.75TAVPASAAATPAAYY51 pKa = 8.82TEE53 pKa = 4.2YY54 pKa = 10.79AAARR58 pKa = 11.84VNVASDD64 pKa = 3.13SGEE67 pKa = 4.15PLSLDD72 pKa = 2.94AVTNVPTSEE81 pKa = 4.04TDD83 pKa = 3.07EE84 pKa = 4.4PAALL88 pKa = 3.94

Molecular weight: 8.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q6M6V1|A0A0Q6M6V1_9BURK HTH lacI-type domain-containing protein OS=Rhizobacter sp. Root29 OX=1736511 GN=ASC88_24700 PE=4 SV=1
MM1 pKa = 7.15VVTTRR6 pKa = 11.84APLPHH11 pKa = 7.25RR12 pKa = 11.84SGQRR16 pKa = 11.84PHH18 pKa = 6.41LRR20 pKa = 11.84LRR22 pKa = 11.84LRR24 pKa = 11.84LRR26 pKa = 11.84PHH28 pKa = 6.63PHH30 pKa = 6.22PHH32 pKa = 5.51PHH34 pKa = 6.49LRR36 pKa = 11.84PHH38 pKa = 6.52PHH40 pKa = 5.72PHH42 pKa = 6.33LRR44 pKa = 11.84PHH46 pKa = 6.92LRR48 pKa = 11.84PHH50 pKa = 5.41PHH52 pKa = 5.56LRR54 pKa = 11.84LRR56 pKa = 11.84LRR58 pKa = 11.84LHH60 pKa = 6.36LHH62 pKa = 6.02LHH64 pKa = 5.91LHH66 pKa = 6.0LHH68 pKa = 6.0LHH70 pKa = 6.0LHH72 pKa = 5.92LHH74 pKa = 5.31LHH76 pKa = 5.7LRR78 pKa = 11.84LRR80 pKa = 11.84LHH82 pKa = 5.65LHH84 pKa = 5.1LRR86 pKa = 11.84LRR88 pKa = 11.84LRR90 pKa = 11.84LRR92 pKa = 11.84LRR94 pKa = 11.84LRR96 pKa = 11.84LRR98 pKa = 11.84LRR100 pKa = 11.84LRR102 pKa = 11.84PLCKK106 pKa = 10.1SPGPIQPPSIGEE118 pKa = 4.07RR119 pKa = 11.84PSARR123 pKa = 11.84PSS125 pKa = 3.02

Molecular weight: 15.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5611

0

5611

1878270

29

3002

334.7

36.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.446 ± 0.05

0.877 ± 0.009

5.533 ± 0.023

4.929 ± 0.028

3.435 ± 0.019

8.508 ± 0.032

2.261 ± 0.017

3.889 ± 0.02

2.854 ± 0.028

10.957 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.266 ± 0.015

2.409 ± 0.022

5.429 ± 0.026

3.944 ± 0.021

7.314 ± 0.033

5.437 ± 0.025

5.212 ± 0.028

7.714 ± 0.026

1.545 ± 0.014

2.039 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski