Beet soil-borne mosaic virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Hepelivirales; Benyviridae; Benyvirus

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9IF44|Q9IF44_9VIRU RNA-dependent RNA polymerase OS=Beet soil-borne mosaic virus OX=76343 PE=4 SV=1
MM1 pKa = 8.03DD2 pKa = 6.43LNTMMPAFNVAYY14 pKa = 8.8WDD16 pKa = 4.22GVHH19 pKa = 6.58APYY22 pKa = 10.25VVKK25 pKa = 10.73RR26 pKa = 11.84MMHH29 pKa = 5.02EE30 pKa = 3.87VVMNVGPAGFICYY43 pKa = 7.92PLPVDD48 pKa = 5.47FDD50 pKa = 5.11LNDD53 pKa = 3.33TGVIHH58 pKa = 6.77NFAYY62 pKa = 10.25HH63 pKa = 5.85NRR65 pKa = 11.84VKK67 pKa = 10.24TMRR70 pKa = 11.84LFVGIQNNCSEE81 pKa = 4.35WVYY84 pKa = 10.85GRR86 pKa = 11.84ARR88 pKa = 11.84FVVFSTSAISPWVNNGCMSLFSPFVGVNSPIDD120 pKa = 3.65RR121 pKa = 11.84NLLRR125 pKa = 11.84RR126 pKa = 11.84DD127 pKa = 3.25SRR129 pKa = 11.84GVSVLWDD136 pKa = 3.03RR137 pKa = 11.84VYY139 pKa = 10.79RR140 pKa = 11.84VNRR143 pKa = 11.84GTQLFVDD150 pKa = 3.84EE151 pKa = 4.82TFNFIGPGNYY161 pKa = 7.85PAQVGEE167 pKa = 4.63NYY169 pKa = 10.16PSATTYY175 pKa = 11.26DD176 pKa = 4.26SIYY179 pKa = 9.46VACVTDD185 pKa = 4.52WIDD188 pKa = 3.54NNVFRR193 pKa = 11.84LTSDD197 pKa = 3.2SVGWFHH203 pKa = 7.59SGLDD207 pKa = 3.67DD208 pKa = 4.6GPRR211 pKa = 11.84LAFGQGLNAPDD222 pKa = 4.79DD223 pKa = 4.92DD224 pKa = 5.77GDD226 pKa = 4.24GVVGDD231 pKa = 5.23DD232 pKa = 4.86DD233 pKa = 5.07VDD235 pKa = 3.72VDD237 pKa = 4.9GEE239 pKa = 4.56NIDD242 pKa = 3.83EE243 pKa = 5.05DD244 pKa = 5.11ADD246 pKa = 4.05VMDD249 pKa = 5.99DD250 pKa = 4.46ANTDD254 pKa = 3.52DD255 pKa = 5.05GDD257 pKa = 3.59

Molecular weight: 28.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O72593|O72593_9VIRU 8kDa protein OS=Beet soil-borne mosaic virus OX=76343 PE=4 SV=1
MM1 pKa = 7.3SRR3 pKa = 11.84EE4 pKa = 3.36ITARR8 pKa = 11.84ANKK11 pKa = 9.43NVPIVVGVCVVAFFVLLAFMQQKK34 pKa = 10.37HH35 pKa = 5.0KK36 pKa = 8.76THH38 pKa = 6.44SGGDD42 pKa = 3.43YY43 pKa = 10.74GVPTFSNGGKK53 pKa = 9.9YY54 pKa = 9.73RR55 pKa = 11.84DD56 pKa = 3.9GTRR59 pKa = 11.84SADD62 pKa = 3.47FNSNNHH68 pKa = 5.99RR69 pKa = 11.84AYY71 pKa = 10.32GCGGSKK77 pKa = 10.41SSVTGKK83 pKa = 10.29VGQQLLVLALVVAVFVLFMRR103 pKa = 11.84GCWSSPEE110 pKa = 4.39HH111 pKa = 6.31ICNGSCGG118 pKa = 3.53

Molecular weight: 12.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

4181

68

2117

418.1

46.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.175 ± 0.601

1.961 ± 0.471

6.649 ± 0.777

4.855 ± 0.532

4.831 ± 0.35

6.817 ± 0.698

2.009 ± 0.2

4.712 ± 0.433

5.142 ± 0.786

9.137 ± 0.774

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.631 ± 0.226

4.951 ± 0.429

3.803 ± 0.335

2.583 ± 0.368

5.429 ± 0.35

7.917 ± 0.286

5.214 ± 0.545

9.352 ± 0.812

1.722 ± 0.203

3.109 ± 0.317
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski