Rhodobacteraceae bacterium PD-2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; unclassified Rhodobacteraceae

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4616 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4ERK4|A0A0B4ERK4_9RHOB 3-isopropylmalate dehydratase (Fragment) OS=Rhodobacteraceae bacterium PD-2 OX=1169855 GN=P279_30875 PE=3 SV=1
MM1 pKa = 7.16ATYY4 pKa = 10.36VYY6 pKa = 10.85DD7 pKa = 3.75GTSLNNLILDD17 pKa = 4.12PASGFDD23 pKa = 3.48VYY25 pKa = 11.54DD26 pKa = 3.97DD27 pKa = 4.15SFYY30 pKa = 11.43SSTLGDD36 pKa = 3.44RR37 pKa = 11.84FDD39 pKa = 4.41NLFDD43 pKa = 3.66LTGITAYY50 pKa = 10.75VDD52 pKa = 3.38GSIFEE57 pKa = 4.25MLAGNDD63 pKa = 3.35QFLGSEE69 pKa = 3.88LGEE72 pKa = 3.96NVFGGEE78 pKa = 4.55GNDD81 pKa = 3.43SLTGNGGNDD90 pKa = 3.89SLWGGLGADD99 pKa = 3.9TLRR102 pKa = 11.84GGSGNDD108 pKa = 3.28QLVGDD113 pKa = 4.33QGVDD117 pKa = 3.37LLDD120 pKa = 4.17GGEE123 pKa = 4.25GNDD126 pKa = 3.71LFLFGSGGDD135 pKa = 3.56VDD137 pKa = 3.82ILRR140 pKa = 11.84GGSGTDD146 pKa = 2.53IGYY149 pKa = 10.77YY150 pKa = 10.04SGDD153 pKa = 3.64YY154 pKa = 10.51LQGLIVDD161 pKa = 4.48AASSIEE167 pKa = 3.92VLHH170 pKa = 5.72QNSYY174 pKa = 9.83NGYY177 pKa = 9.36YY178 pKa = 10.37GDD180 pKa = 3.64NAANLFDD187 pKa = 3.72VSGVQSYY194 pKa = 10.71VGAADD199 pKa = 5.01FYY201 pKa = 11.45LGGGNDD207 pKa = 3.54TFVGSDD213 pKa = 3.25VAEE216 pKa = 3.94WAMGQFGNDD225 pKa = 3.14TLLGNGGDD233 pKa = 4.55DD234 pKa = 4.76RR235 pKa = 11.84LQGWEE240 pKa = 4.87DD241 pKa = 4.01DD242 pKa = 3.75DD243 pKa = 4.93LLRR246 pKa = 11.84GGAGADD252 pKa = 3.52TLEE255 pKa = 4.91GGSGTDD261 pKa = 3.07TADD264 pKa = 3.51YY265 pKa = 9.14TGSSSWVNVSLLTGYY280 pKa = 9.18TSGGHH285 pKa = 5.43AQGDD289 pKa = 3.97VLSGIEE295 pKa = 4.1NLTGSAHH302 pKa = 6.87GDD304 pKa = 3.39RR305 pKa = 11.84LSGDD309 pKa = 2.9HH310 pKa = 6.47GANVLRR316 pKa = 11.84GQAGDD321 pKa = 4.01DD322 pKa = 3.29ILRR325 pKa = 11.84GRR327 pKa = 11.84GGADD331 pKa = 3.05TLEE334 pKa = 4.67GGAGSDD340 pKa = 2.85IADD343 pKa = 3.74YY344 pKa = 11.01SEE346 pKa = 4.06SAGWVNVSLISGYY359 pKa = 11.01AGGGAGSHH367 pKa = 7.15AIGDD371 pKa = 3.54VWIGIEE377 pKa = 4.05NLIGSDD383 pKa = 3.67FADD386 pKa = 4.52RR387 pKa = 11.84LNGDD391 pKa = 3.06NGEE394 pKa = 4.21NVLEE398 pKa = 4.42GRR400 pKa = 11.84AGGDD404 pKa = 2.7IFNGYY409 pKa = 9.22GGSDD413 pKa = 3.4TLSYY417 pKa = 10.56ASSVAWVNVSLLTGYY432 pKa = 8.81TGGGAGNHH440 pKa = 6.41AAGDD444 pKa = 3.57SWTSIEE450 pKa = 4.03NLRR453 pKa = 11.84GSAHH457 pKa = 7.32ADD459 pKa = 3.47RR460 pKa = 11.84LNGDD464 pKa = 3.1HH465 pKa = 6.82GANVLEE471 pKa = 5.01GGAGGDD477 pKa = 3.07IVNGNGGSDD486 pKa = 3.43TLSYY490 pKa = 10.89ASSEE494 pKa = 3.7AWVNVSLLTGYY505 pKa = 8.81TGGGAGNHH513 pKa = 6.41AAGDD517 pKa = 3.57SWTSIEE523 pKa = 4.03NLRR526 pKa = 11.84GSAHH530 pKa = 7.32ADD532 pKa = 3.47RR533 pKa = 11.84LNGDD537 pKa = 3.1HH538 pKa = 6.82GANVLEE544 pKa = 5.01GGAGGDD550 pKa = 3.07IVNGNGGSDD559 pKa = 3.43TLSYY563 pKa = 10.89ASSEE567 pKa = 3.7AWVNVSLLTGYY578 pKa = 8.81TGGGAGNHH586 pKa = 6.41AAGDD590 pKa = 3.57SWTSIEE596 pKa = 4.03NLRR599 pKa = 11.84GSAHH603 pKa = 7.32ADD605 pKa = 3.47RR606 pKa = 11.84LNGDD610 pKa = 3.1HH611 pKa = 6.82GANVLEE617 pKa = 5.01GGAGGDD623 pKa = 3.07IVNGNGGSDD632 pKa = 3.43TLSYY636 pKa = 10.89ASSEE640 pKa = 3.7AWVNVSLLTGYY651 pKa = 8.81TGGGAGNHH659 pKa = 6.41AAGDD663 pKa = 3.57SWTSIEE669 pKa = 4.03NLRR672 pKa = 11.84GSAHH676 pKa = 7.32ADD678 pKa = 3.47RR679 pKa = 11.84LNGDD683 pKa = 3.1HH684 pKa = 6.82GANVLEE690 pKa = 5.01GGAGGDD696 pKa = 3.07IVNGNGGSDD705 pKa = 3.43TLSYY709 pKa = 10.56ASSVAWVNVSLLTGYY724 pKa = 8.81TGGGAGNHH732 pKa = 6.41AAGDD736 pKa = 3.57SWTSIEE742 pKa = 4.03NLRR745 pKa = 11.84GSAHH749 pKa = 7.32ADD751 pKa = 3.47RR752 pKa = 11.84LNGDD756 pKa = 3.1HH757 pKa = 6.82GANVLEE763 pKa = 4.91GGAGNDD769 pKa = 3.04ILRR772 pKa = 11.84GNGGIDD778 pKa = 3.04TFVFADD784 pKa = 4.05GFGSDD789 pKa = 5.02SVLDD793 pKa = 3.84YY794 pKa = 11.64QNGSEE799 pKa = 4.84KK800 pKa = 10.58FDD802 pKa = 3.8FTDD805 pKa = 3.11HH806 pKa = 6.82AGVSGFGDD814 pKa = 3.7LTVSASGADD823 pKa = 3.55ALIADD828 pKa = 4.68GFGNQITVTGAAGLIDD844 pKa = 3.67ATDD847 pKa = 4.35FIFF850 pKa = 5.29

Molecular weight: 85.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4FJ82|A0A0B4FJ82_9RHOB Flagellar basal body rod protein FlgB (Fragment) OS=Rhodobacteraceae bacterium PD-2 OX=1169855 GN=flgB PE=3 SV=1
MM1 pKa = 7.45NSATIFNFRR10 pKa = 11.84RR11 pKa = 11.84GLPAVFHH18 pKa = 5.55VRR20 pKa = 11.84RR21 pKa = 11.84FIIVGPAHH29 pKa = 7.03LGGFLLRR36 pKa = 11.84TPMHH40 pKa = 6.78GPQIHH45 pKa = 5.47VLKK48 pKa = 10.59AALPLWHH55 pKa = 6.9MSSKK59 pKa = 10.01HH60 pKa = 5.13RR61 pKa = 11.84HH62 pKa = 4.43RR63 pKa = 11.84QAPRR67 pKa = 11.84RR68 pKa = 11.84VSAQQ72 pKa = 2.96

Molecular weight: 8.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4616

0

4616

1370329

34

3204

296.9

32.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.64 ± 0.052

0.917 ± 0.011

5.979 ± 0.029

5.986 ± 0.036

3.624 ± 0.024

8.955 ± 0.036

2.054 ± 0.018

4.806 ± 0.031

2.844 ± 0.029

10.233 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.812 ± 0.018

2.38 ± 0.02

5.186 ± 0.027

3.136 ± 0.021

6.986 ± 0.044

4.937 ± 0.028

5.523 ± 0.037

7.428 ± 0.032

1.4 ± 0.016

2.175 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski