Sanxia narna-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KIL2|A0A1L3KIL2_9VIRU RNA-dependent RNA polymerase OS=Sanxia narna-like virus 1 OX=1923360 PE=4 SV=1
MM1 pKa = 7.87KK2 pKa = 10.2YY3 pKa = 11.09NLMTKK8 pKa = 10.1SKK10 pKa = 9.14TKK12 pKa = 10.66SNQKK16 pKa = 8.64PAQRR20 pKa = 11.84AKK22 pKa = 10.31PRR24 pKa = 11.84RR25 pKa = 11.84SRR27 pKa = 11.84QRR29 pKa = 11.84ANKK32 pKa = 8.91VQDD35 pKa = 3.52GLDD38 pKa = 3.88GYY40 pKa = 10.9GLAAARR46 pKa = 11.84MYY48 pKa = 11.19ADD50 pKa = 3.59PCGADD55 pKa = 3.75LVPTVYY61 pKa = 10.65SGDD64 pKa = 3.18RR65 pKa = 11.84GYY67 pKa = 10.78INRR70 pKa = 11.84FVANASVAITAGDD83 pKa = 3.44TSLILLIKK91 pKa = 10.01PGNQTAFFRR100 pKa = 11.84ADD102 pKa = 3.58TAPTTPGAIGYY113 pKa = 8.69DD114 pKa = 3.2NSIFPGRR121 pKa = 11.84SFYY124 pKa = 10.8TSNASKK130 pKa = 10.47ARR132 pKa = 11.84CAGYY136 pKa = 10.11CINVRR141 pKa = 11.84PNSAPNNATGTIYY154 pKa = 10.6FGNINASAVPNLASITASGLIPLLSEE180 pKa = 4.77SVSCSQALMQPLEE193 pKa = 4.6VKK195 pKa = 9.69WSPGGFDD202 pKa = 4.8DD203 pKa = 5.45RR204 pKa = 11.84YY205 pKa = 9.18NTIGTITDD213 pKa = 3.78DD214 pKa = 4.27DD215 pKa = 4.35SDD217 pKa = 5.02RR218 pKa = 11.84NVLCIVVLGLPAASGMQIRR237 pKa = 11.84QTVITEE243 pKa = 4.18WSPANALTVTNDD255 pKa = 2.79ATAVKK260 pKa = 10.1PSVCDD265 pKa = 3.29KK266 pKa = 11.31DD267 pKa = 4.55CILRR271 pKa = 11.84HH272 pKa = 6.14LKK274 pKa = 10.59RR275 pKa = 11.84KK276 pKa = 10.25DD277 pKa = 3.5SAWWWKK283 pKa = 10.32LGHH286 pKa = 6.57KK287 pKa = 10.23VLDD290 pKa = 3.67VGKK293 pKa = 9.35SVGMGYY299 pKa = 8.19YY300 pKa = 9.81TGGAVGALGAAVRR313 pKa = 11.84FMM315 pKa = 4.85

Molecular weight:
33.66 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KIL2|A0A1L3KIL2_9VIRU RNA-dependent RNA polymerase OS=Sanxia narna-like virus 1 OX=1923360 PE=4 SV=1
MM1 pKa = 6.93TPQMRR6 pKa = 11.84EE7 pKa = 3.93HH8 pKa = 6.44NSARR12 pKa = 11.84SRR14 pKa = 11.84APRR17 pKa = 11.84MSKK20 pKa = 10.56DD21 pKa = 3.05MHH23 pKa = 6.6LRR25 pKa = 11.84SPLNRR30 pKa = 11.84GPKK33 pKa = 10.07GADD36 pKa = 2.98FLVRR40 pKa = 11.84IPEE43 pKa = 4.22LVADD47 pKa = 4.59PVQVSKK53 pKa = 11.23LSAKK57 pKa = 9.69GVWHH61 pKa = 6.86KK62 pKa = 11.23SDD64 pKa = 3.35NALVEE69 pKa = 4.13TFRR72 pKa = 11.84LIGYY76 pKa = 7.09EE77 pKa = 4.23FKK79 pKa = 11.04NVLCPSPPTSSVPEE93 pKa = 4.03VMSVLKK99 pKa = 10.7NWVAFWLPWFLGDD112 pKa = 4.06DD113 pKa = 3.88TPPQRR118 pKa = 11.84FNPLSLCSPGFRR130 pKa = 11.84SFIRR134 pKa = 11.84NRR136 pKa = 11.84KK137 pKa = 5.37TSKK140 pKa = 8.77TGKK143 pKa = 8.9SRR145 pKa = 11.84KK146 pKa = 9.08IRR148 pKa = 11.84IGALLLYY155 pKa = 9.99SKK157 pKa = 10.74RR158 pKa = 11.84LFPSFTGEE166 pKa = 3.91MVRR169 pKa = 11.84EE170 pKa = 3.99KK171 pKa = 10.98VKK173 pKa = 10.82EE174 pKa = 3.95FGDD177 pKa = 3.49AVTRR181 pKa = 11.84VSPGDD186 pKa = 3.52LPRR189 pKa = 11.84KK190 pKa = 8.54RR191 pKa = 11.84KK192 pKa = 8.78MYY194 pKa = 10.79GSIEE198 pKa = 3.92QSIDD202 pKa = 2.97EE203 pKa = 4.29FLQGGRR209 pKa = 11.84MVADD213 pKa = 3.43YY214 pKa = 10.28SKK216 pKa = 10.59PFAPSTSACYY226 pKa = 9.63EE227 pKa = 3.77KK228 pKa = 10.87SRR230 pKa = 11.84AEE232 pKa = 4.01GGLQRR237 pKa = 11.84FVADD241 pKa = 4.45NILQEE246 pKa = 4.43TVEE249 pKa = 4.36SRR251 pKa = 11.84FFYY254 pKa = 10.82DD255 pKa = 4.68DD256 pKa = 4.91LNHH259 pKa = 6.37IWKK262 pKa = 10.32CSEE265 pKa = 4.31DD266 pKa = 4.05DD267 pKa = 3.79LTFGTPIGLWNEE279 pKa = 3.96FLDD282 pKa = 3.73IMFRR286 pKa = 11.84RR287 pKa = 11.84AMSEE291 pKa = 3.59TGLPDD296 pKa = 3.69QEE298 pKa = 4.43WKK300 pKa = 10.5RR301 pKa = 11.84LPVQATGLTEE311 pKa = 3.93PLKK314 pKa = 11.01VRR316 pKa = 11.84IVTKK320 pKa = 10.42SNWFLQLLTPIQKK333 pKa = 9.54AWHH336 pKa = 5.81GAMRR340 pKa = 11.84SHH342 pKa = 8.26PIYY345 pKa = 10.45QLIGGIPVEE354 pKa = 4.11QALLGLDD361 pKa = 3.79LQRR364 pKa = 11.84KK365 pKa = 7.08QKK367 pKa = 10.27VVSGDD372 pKa = 3.3YY373 pKa = 10.71SAATDD378 pKa = 4.13NIFLEE383 pKa = 4.38YY384 pKa = 10.23TEE386 pKa = 4.53YY387 pKa = 10.87AAKK390 pKa = 10.82AMLEE394 pKa = 3.97RR395 pKa = 11.84TDD397 pKa = 5.67FSFLDD402 pKa = 3.68PQLVQFVPWIKK413 pKa = 10.64KK414 pKa = 9.71LVIHH418 pKa = 5.79SLVRR422 pKa = 11.84SSLEE426 pKa = 3.94LKK428 pKa = 10.72GMEE431 pKa = 4.66PKK433 pKa = 10.65DD434 pKa = 3.27ITRR437 pKa = 11.84GQMMGHH443 pKa = 6.87ILSFPLLCLINRR455 pKa = 11.84SASVMAIPRR464 pKa = 11.84EE465 pKa = 3.56RR466 pKa = 11.84FMRR469 pKa = 11.84INGDD473 pKa = 3.51DD474 pKa = 3.6VLFPANPTEE483 pKa = 3.79YY484 pKa = 10.17RR485 pKa = 11.84KK486 pKa = 9.42WKK488 pKa = 10.05RR489 pKa = 11.84ATASVGLEE497 pKa = 3.59FSLGKK502 pKa = 10.21NYY504 pKa = 10.31YY505 pKa = 10.29SRR507 pKa = 11.84DD508 pKa = 3.39LALVNSTYY516 pKa = 10.73CVWDD520 pKa = 3.68KK521 pKa = 11.68ASARR525 pKa = 11.84WKK527 pKa = 10.13VLSVPNVGLLNMPMEE542 pKa = 4.34KK543 pKa = 10.19QIDD546 pKa = 4.16TEE548 pKa = 4.19TGRR551 pKa = 11.84QIMPWEE557 pKa = 3.96VLAQNWRR564 pKa = 11.84EE565 pKa = 3.76FSRR568 pKa = 11.84FSTKK572 pKa = 8.66KK573 pKa = 7.22TQSTFVRR580 pKa = 11.84LFLKK584 pKa = 10.18HH585 pKa = 5.77YY586 pKa = 8.58PILRR590 pKa = 11.84GFPGPIFGPTQWGGLGAPIPDD611 pKa = 2.98GHH613 pKa = 6.73KK614 pKa = 9.4FTRR617 pKa = 11.84NQLMWMNAHH626 pKa = 6.77RR627 pKa = 11.84LGMFSYY633 pKa = 10.74LQGTRR638 pKa = 11.84TDD640 pKa = 3.55YY641 pKa = 11.45SRR643 pKa = 11.84LSEE646 pKa = 4.35MYY648 pKa = 9.2AQQIEE653 pKa = 4.66KK654 pKa = 9.82YY655 pKa = 8.13VTGIYY660 pKa = 8.88EE661 pKa = 3.94WRR663 pKa = 11.84EE664 pKa = 3.69PEE666 pKa = 4.13YY667 pKa = 11.11GDD669 pKa = 3.93SFGPLEE675 pKa = 4.8SGVFMDD681 pKa = 5.75PYY683 pKa = 11.05SKK685 pKa = 10.92DD686 pKa = 2.7GGYY689 pKa = 10.56AGQVMALRR697 pKa = 11.84RR698 pKa = 11.84WVVDD702 pKa = 3.82ASSMKK707 pKa = 10.05HH708 pKa = 5.02IKK710 pKa = 10.14IFGARR715 pKa = 11.84RR716 pKa = 11.84WNQYY720 pKa = 10.18KK721 pKa = 10.72LSLKK725 pKa = 9.87TGIPPLPQFYY735 pKa = 10.46LDD737 pKa = 4.09KK738 pKa = 10.92LRR740 pKa = 11.84SGDD743 pKa = 3.15IYY745 pKa = 10.81FPRR748 pKa = 11.84PGWNRR753 pKa = 11.84SRR755 pKa = 11.84DD756 pKa = 3.78MTGSRR761 pKa = 11.84YY762 pKa = 8.91EE763 pKa = 3.98DD764 pKa = 3.45RR765 pKa = 11.84ATYY768 pKa = 10.01LHH770 pKa = 7.07EE771 pKa = 4.81IFPSTLKK778 pKa = 10.91NN779 pKa = 3.34

Molecular weight:
89.53 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1094

315

779

547.0

61.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.13 ± 2.309

1.188 ± 0.517

5.21 ± 0.411

3.931 ± 1.49

4.57 ± 1.016

7.495 ± 0.697

1.371 ± 0.368

4.845 ± 0.276

5.941 ± 0.272

8.867 ± 0.942

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.199 ± 0.489

4.022 ± 1.005

6.307 ± 0.455

3.565 ± 0.354

7.13 ± 0.708

7.861 ± 0.038

5.484 ± 0.75

5.85 ± 0.409

2.377 ± 0.395

3.656 ± 0.077

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski