Amino acid dipepetide frequency for Streptococcus phage phi-SsuHCJ3_rum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.417AlaAla: 3.417 ± 0.901
0.707AlaCys: 0.707 ± 0.179
3.476AlaAsp: 3.476 ± 0.466
3.888AlaGlu: 3.888 ± 0.45
2.533AlaPhe: 2.533 ± 0.315
4.359AlaGly: 4.359 ± 0.779
0.648AlaHis: 0.648 ± 0.211
5.655AlaIle: 5.655 ± 0.65
5.243AlaLys: 5.243 ± 0.636
5.125AlaLeu: 5.125 ± 0.856
1.826AlaMet: 1.826 ± 0.301
3.299AlaAsn: 3.299 ± 0.412
1.767AlaPro: 1.767 ± 0.348
2.651AlaGln: 2.651 ± 0.641
2.71AlaArg: 2.71 ± 0.409
4.536AlaSer: 4.536 ± 0.627
4.183AlaThr: 4.183 ± 0.497
3.711AlaVal: 3.711 ± 0.524
0.648AlaTrp: 0.648 ± 0.244
2.769AlaTyr: 2.769 ± 0.402
0.0AlaXaa: 0.0 ± 0.0
Cys
0.589CysAla: 0.589 ± 0.197
0.295CysCys: 0.295 ± 0.127
0.412CysAsp: 0.412 ± 0.138
0.589CysGlu: 0.589 ± 0.157
0.236CysPhe: 0.236 ± 0.117
0.707CysGly: 0.707 ± 0.165
0.471CysHis: 0.471 ± 0.167
0.53CysIle: 0.53 ± 0.222
0.53CysLys: 0.53 ± 0.246
0.707CysLeu: 0.707 ± 0.246
0.059CysMet: 0.059 ± 0.057
0.471CysAsn: 0.471 ± 0.177
0.53CysPro: 0.53 ± 0.164
0.884CysGln: 0.884 ± 0.244
0.589CysArg: 0.589 ± 0.217
0.589CysSer: 0.589 ± 0.249
0.295CysThr: 0.295 ± 0.138
0.53CysVal: 0.53 ± 0.203
0.0CysTrp: 0.0 ± 0.0
0.589CysTyr: 0.589 ± 0.209
0.0CysXaa: 0.0 ± 0.0
Asp
3.004AspAla: 3.004 ± 0.427
0.53AspCys: 0.53 ± 0.189
3.358AspAsp: 3.358 ± 0.569
5.891AspGlu: 5.891 ± 0.79
3.358AspPhe: 3.358 ± 0.411
4.654AspGly: 4.654 ± 0.585
1.296AspHis: 1.296 ± 0.28
4.831AspIle: 4.831 ± 0.407
4.242AspLys: 4.242 ± 0.376
4.418AspLeu: 4.418 ± 0.739
1.885AspMet: 1.885 ± 0.366
2.828AspAsn: 2.828 ± 0.324
1.708AspPro: 1.708 ± 0.356
1.591AspGln: 1.591 ± 0.269
2.592AspArg: 2.592 ± 0.495
3.476AspSer: 3.476 ± 0.564
2.946AspThr: 2.946 ± 0.391
3.181AspVal: 3.181 ± 0.471
0.943AspTrp: 0.943 ± 0.219
2.887AspTyr: 2.887 ± 0.58
0.0AspXaa: 0.0 ± 0.0
Glu
4.359GluAla: 4.359 ± 0.41
0.648GluCys: 0.648 ± 0.194
4.831GluAsp: 4.831 ± 0.595
5.832GluGlu: 5.832 ± 0.87
2.474GluPhe: 2.474 ± 0.446
4.713GluGly: 4.713 ± 0.427
1.355GluHis: 1.355 ± 0.281
5.007GluIle: 5.007 ± 0.483
6.244GluLys: 6.244 ± 0.718
8.13GluLeu: 8.13 ± 0.667
2.533GluMet: 2.533 ± 0.517
3.829GluAsn: 3.829 ± 0.567
1.649GluPro: 1.649 ± 0.378
3.77GluGln: 3.77 ± 0.466
3.122GluArg: 3.122 ± 0.393
3.299GluSer: 3.299 ± 0.388
3.947GluThr: 3.947 ± 0.54
4.418GluVal: 4.418 ± 0.544
0.825GluTrp: 0.825 ± 0.231
2.651GluTyr: 2.651 ± 0.644
0.0GluXaa: 0.0 ± 0.0
Phe
2.533PheAla: 2.533 ± 0.393
0.589PheCys: 0.589 ± 0.188
3.063PheAsp: 3.063 ± 0.477
3.122PheGlu: 3.122 ± 0.443
1.414PhePhe: 1.414 ± 0.328
2.297PheGly: 2.297 ± 0.329
0.766PheHis: 0.766 ± 0.23
2.415PheIle: 2.415 ± 0.337
3.004PheLys: 3.004 ± 0.556
3.594PheLeu: 3.594 ± 0.608
0.766PheMet: 0.766 ± 0.205
2.003PheAsn: 2.003 ± 0.274
1.001PhePro: 1.001 ± 0.248
1.414PheGln: 1.414 ± 0.316
1.944PheArg: 1.944 ± 0.321
2.062PheSer: 2.062 ± 0.323
2.651PheThr: 2.651 ± 0.416
1.767PheVal: 1.767 ± 0.352
0.648PheTrp: 0.648 ± 0.193
2.297PheTyr: 2.297 ± 0.338
0.0PheXaa: 0.0 ± 0.0
Gly
3.417GlyAla: 3.417 ± 0.521
0.412GlyCys: 0.412 ± 0.152
4.242GlyAsp: 4.242 ± 0.595
4.124GlyGlu: 4.124 ± 0.528
2.828GlyPhe: 2.828 ± 0.408
3.829GlyGly: 3.829 ± 0.558
2.239GlyHis: 2.239 ± 0.7
5.832GlyIle: 5.832 ± 0.792
4.595GlyLys: 4.595 ± 0.512
5.95GlyLeu: 5.95 ± 0.666
2.121GlyMet: 2.121 ± 0.375
3.122GlyAsn: 3.122 ± 0.493
0.766GlyPro: 0.766 ± 0.182
2.828GlyGln: 2.828 ± 0.476
3.535GlyArg: 3.535 ± 0.385
4.006GlySer: 4.006 ± 0.443
3.829GlyThr: 3.829 ± 0.504
3.888GlyVal: 3.888 ± 0.517
0.648GlyTrp: 0.648 ± 0.155
3.299GlyTyr: 3.299 ± 0.433
0.0GlyXaa: 0.0 ± 0.0
His
0.943HisAla: 0.943 ± 0.213
0.353HisCys: 0.353 ± 0.127
1.414HisAsp: 1.414 ± 0.355
1.06HisGlu: 1.06 ± 0.206
0.943HisPhe: 0.943 ± 0.178
1.826HisGly: 1.826 ± 0.33
1.001HisHis: 1.001 ± 0.325
1.06HisIle: 1.06 ± 0.235
0.943HisLys: 0.943 ± 0.242
2.003HisLeu: 2.003 ± 0.327
0.53HisMet: 0.53 ± 0.195
0.884HisAsn: 0.884 ± 0.202
1.178HisPro: 1.178 ± 0.269
1.001HisGln: 1.001 ± 0.286
1.414HisArg: 1.414 ± 0.306
1.237HisSer: 1.237 ± 0.423
0.943HisThr: 0.943 ± 0.28
1.119HisVal: 1.119 ± 0.238
0.236HisTrp: 0.236 ± 0.117
0.884HisTyr: 0.884 ± 0.285
0.0HisXaa: 0.0 ± 0.0
Ile
5.302IleAla: 5.302 ± 0.459
0.884IleCys: 0.884 ± 0.253
5.243IleAsp: 5.243 ± 0.494
4.772IleGlu: 4.772 ± 0.726
2.062IlePhe: 2.062 ± 0.445
4.89IleGly: 4.89 ± 0.679
1.001IleHis: 1.001 ± 0.171
4.3IleIle: 4.3 ± 0.518
4.654IleLys: 4.654 ± 0.501
6.186IleLeu: 6.186 ± 0.617
1.119IleMet: 1.119 ± 0.302
3.063IleAsn: 3.063 ± 0.427
2.356IlePro: 2.356 ± 0.286
2.533IleGln: 2.533 ± 0.372
3.299IleArg: 3.299 ± 0.335
4.831IleSer: 4.831 ± 0.698
4.183IleThr: 4.183 ± 0.704
4.948IleVal: 4.948 ± 0.593
1.237IleTrp: 1.237 ± 0.285
3.063IleTyr: 3.063 ± 0.547
0.0IleXaa: 0.0 ± 0.0
Lys
5.302LysAla: 5.302 ± 0.615
0.471LysCys: 0.471 ± 0.192
4.183LysAsp: 4.183 ± 0.433
5.891LysGlu: 5.891 ± 0.557
2.415LysPhe: 2.415 ± 0.411
5.361LysGly: 5.361 ± 0.634
1.767LysHis: 1.767 ± 0.282
4.772LysIle: 4.772 ± 0.465
5.655LysLys: 5.655 ± 0.796
5.95LysLeu: 5.95 ± 0.582
2.239LysMet: 2.239 ± 0.409
3.181LysAsn: 3.181 ± 0.468
2.121LysPro: 2.121 ± 0.405
3.299LysGln: 3.299 ± 0.488
3.535LysArg: 3.535 ± 0.535
4.418LysSer: 4.418 ± 0.49
4.242LysThr: 4.242 ± 0.49
5.302LysVal: 5.302 ± 0.585
1.001LysTrp: 1.001 ± 0.22
2.533LysTyr: 2.533 ± 0.531
0.0LysXaa: 0.0 ± 0.0
Leu
6.127LeuAla: 6.127 ± 0.651
0.707LeuCys: 0.707 ± 0.244
4.772LeuAsp: 4.772 ± 0.547
7.835LeuGlu: 7.835 ± 0.726
3.24LeuPhe: 3.24 ± 0.478
5.891LeuGly: 5.891 ± 0.707
1.708LeuHis: 1.708 ± 0.279
5.361LeuIle: 5.361 ± 0.533
7.01LeuLys: 7.01 ± 0.635
8.189LeuLeu: 8.189 ± 0.757
1.944LeuMet: 1.944 ± 0.271
4.3LeuAsn: 4.3 ± 0.48
2.946LeuPro: 2.946 ± 0.407
3.122LeuGln: 3.122 ± 0.457
3.77LeuArg: 3.77 ± 0.534
7.246LeuSer: 7.246 ± 0.576
6.539LeuThr: 6.539 ± 0.702
5.655LeuVal: 5.655 ± 0.659
0.707LeuTrp: 0.707 ± 0.157
3.476LeuTyr: 3.476 ± 0.466
0.0LeuXaa: 0.0 ± 0.0
Met
1.826MetAla: 1.826 ± 0.277
0.177MetCys: 0.177 ± 0.104
2.062MetAsp: 2.062 ± 0.44
1.708MetGlu: 1.708 ± 0.388
0.943MetPhe: 0.943 ± 0.292
1.649MetGly: 1.649 ± 0.411
0.236MetHis: 0.236 ± 0.13
1.649MetIle: 1.649 ± 0.366
1.767MetLys: 1.767 ± 0.301
1.944MetLeu: 1.944 ± 0.363
1.237MetMet: 1.237 ± 0.332
0.766MetAsn: 0.766 ± 0.182
0.589MetPro: 0.589 ± 0.197
0.825MetGln: 0.825 ± 0.209
1.296MetArg: 1.296 ± 0.274
2.003MetSer: 2.003 ± 0.394
1.885MetThr: 1.885 ± 0.364
1.414MetVal: 1.414 ± 0.321
0.118MetTrp: 0.118 ± 0.085
0.766MetTyr: 0.766 ± 0.2
0.0MetXaa: 0.0 ± 0.0
Asn
3.829AsnAla: 3.829 ± 0.601
0.295AsnCys: 0.295 ± 0.159
2.769AsnAsp: 2.769 ± 0.324
3.24AsnGlu: 3.24 ± 0.545
1.885AsnPhe: 1.885 ± 0.418
4.065AsnGly: 4.065 ± 0.533
1.355AsnHis: 1.355 ± 0.224
3.476AsnIle: 3.476 ± 0.475
3.299AsnLys: 3.299 ± 0.46
4.418AsnLeu: 4.418 ± 0.616
1.06AsnMet: 1.06 ± 0.247
2.18AsnAsn: 2.18 ± 0.4
2.003AsnPro: 2.003 ± 0.361
2.474AsnGln: 2.474 ± 0.395
2.297AsnArg: 2.297 ± 0.4
2.71AsnSer: 2.71 ± 0.364
2.121AsnThr: 2.121 ± 0.413
2.533AsnVal: 2.533 ± 0.425
1.001AsnTrp: 1.001 ± 0.259
1.414AsnTyr: 1.414 ± 0.25
0.0AsnXaa: 0.0 ± 0.0
Pro
0.943ProAla: 0.943 ± 0.256
0.295ProCys: 0.295 ± 0.119
1.473ProAsp: 1.473 ± 0.325
2.592ProGlu: 2.592 ± 0.443
1.178ProPhe: 1.178 ± 0.267
1.001ProGly: 1.001 ± 0.345
0.825ProHis: 0.825 ± 0.189
1.708ProIle: 1.708 ± 0.304
2.592ProLys: 2.592 ± 0.474
2.71ProLeu: 2.71 ± 0.419
0.648ProMet: 0.648 ± 0.167
1.826ProAsn: 1.826 ± 0.398
0.884ProPro: 0.884 ± 0.289
1.001ProGln: 1.001 ± 0.246
1.532ProArg: 1.532 ± 0.274
2.474ProSer: 2.474 ± 0.36
2.18ProThr: 2.18 ± 0.439
2.356ProVal: 2.356 ± 0.329
0.353ProTrp: 0.353 ± 0.144
1.296ProTyr: 1.296 ± 0.3
0.0ProXaa: 0.0 ± 0.0
Gln
3.77GlnAla: 3.77 ± 0.449
0.412GlnCys: 0.412 ± 0.159
2.297GlnAsp: 2.297 ± 0.337
2.769GlnGlu: 2.769 ± 0.304
1.826GlnPhe: 1.826 ± 0.277
2.297GlnGly: 2.297 ± 0.421
0.471GlnHis: 0.471 ± 0.151
2.946GlnIle: 2.946 ± 0.414
2.71GlnLys: 2.71 ± 0.427
4.065GlnLeu: 4.065 ± 0.499
1.119GlnMet: 1.119 ± 0.272
2.592GlnAsn: 2.592 ± 0.405
1.296GlnPro: 1.296 ± 0.326
1.944GlnGln: 1.944 ± 0.299
1.532GlnArg: 1.532 ± 0.283
2.828GlnSer: 2.828 ± 0.44
2.592GlnThr: 2.592 ± 0.631
3.829GlnVal: 3.829 ± 0.528
0.471GlnTrp: 0.471 ± 0.229
0.943GlnTyr: 0.943 ± 0.272
0.0GlnXaa: 0.0 ± 0.0
Arg
2.356ArgAla: 2.356 ± 0.349
0.589ArgCys: 0.589 ± 0.181
2.533ArgAsp: 2.533 ± 0.427
3.24ArgGlu: 3.24 ± 0.379
2.003ArgPhe: 2.003 ± 0.425
2.533ArgGly: 2.533 ± 0.405
0.707ArgHis: 0.707 ± 0.214
3.417ArgIle: 3.417 ± 0.526
3.77ArgLys: 3.77 ± 0.548
4.536ArgLeu: 4.536 ± 0.494
0.825ArgMet: 0.825 ± 0.245
2.415ArgAsn: 2.415 ± 0.405
1.591ArgPro: 1.591 ± 0.318
2.71ArgGln: 2.71 ± 0.368
2.121ArgArg: 2.121 ± 0.491
2.769ArgSer: 2.769 ± 0.377
2.769ArgThr: 2.769 ± 0.588
2.828ArgVal: 2.828 ± 0.454
0.943ArgTrp: 0.943 ± 0.263
1.885ArgTyr: 1.885 ± 0.316
0.0ArgXaa: 0.0 ± 0.0
Ser
3.947SerAla: 3.947 ± 0.462
0.648SerCys: 0.648 ± 0.186
3.535SerAsp: 3.535 ± 0.464
4.3SerGlu: 4.3 ± 0.524
3.417SerPhe: 3.417 ± 0.551
4.595SerGly: 4.595 ± 0.637
1.296SerHis: 1.296 ± 0.248
4.948SerIle: 4.948 ± 0.534
4.536SerLys: 4.536 ± 0.45
5.184SerLeu: 5.184 ± 0.522
1.296SerMet: 1.296 ± 0.251
2.946SerAsn: 2.946 ± 0.458
2.18SerPro: 2.18 ± 0.337
3.122SerGln: 3.122 ± 0.692
3.004SerArg: 3.004 ± 0.415
5.066SerSer: 5.066 ± 0.745
4.3SerThr: 4.3 ± 0.556
3.829SerVal: 3.829 ± 0.526
0.943SerTrp: 0.943 ± 0.197
2.592SerTyr: 2.592 ± 0.415
0.0SerXaa: 0.0 ± 0.0
Thr
4.359ThrAla: 4.359 ± 0.602
0.118ThrCys: 0.118 ± 0.092
3.004ThrAsp: 3.004 ± 0.415
4.183ThrGlu: 4.183 ± 0.572
2.533ThrPhe: 2.533 ± 0.497
4.477ThrGly: 4.477 ± 0.689
0.766ThrHis: 0.766 ± 0.228
4.477ThrIle: 4.477 ± 0.656
4.477ThrLys: 4.477 ± 0.469
5.891ThrLeu: 5.891 ± 0.516
1.06ThrMet: 1.06 ± 0.259
2.651ThrAsn: 2.651 ± 0.466
1.591ThrPro: 1.591 ± 0.341
2.415ThrGln: 2.415 ± 0.605
2.415ThrArg: 2.415 ± 0.421
4.772ThrSer: 4.772 ± 0.831
4.89ThrThr: 4.89 ± 0.775
4.89ThrVal: 4.89 ± 0.67
0.766ThrTrp: 0.766 ± 0.203
1.885ThrTyr: 1.885 ± 0.385
0.0ThrXaa: 0.0 ± 0.0
Val
4.065ValAla: 4.065 ± 0.612
0.53ValCys: 0.53 ± 0.206
3.711ValAsp: 3.711 ± 0.503
4.477ValGlu: 4.477 ± 0.549
2.18ValPhe: 2.18 ± 0.388
3.24ValGly: 3.24 ± 0.564
1.355ValHis: 1.355 ± 0.245
4.242ValIle: 4.242 ± 0.537
4.654ValLys: 4.654 ± 0.501
6.303ValLeu: 6.303 ± 0.489
1.414ValMet: 1.414 ± 0.281
3.063ValAsn: 3.063 ± 0.48
2.415ValPro: 2.415 ± 0.342
2.474ValGln: 2.474 ± 0.381
3.063ValArg: 3.063 ± 0.58
4.477ValSer: 4.477 ± 0.728
4.065ValThr: 4.065 ± 0.526
3.181ValVal: 3.181 ± 0.496
0.943ValTrp: 0.943 ± 0.236
2.18ValTyr: 2.18 ± 0.443
0.0ValXaa: 0.0 ± 0.0
Trp
0.825TrpAla: 0.825 ± 0.241
0.177TrpCys: 0.177 ± 0.103
0.353TrpAsp: 0.353 ± 0.16
1.237TrpGlu: 1.237 ± 0.266
0.648TrpPhe: 0.648 ± 0.181
0.589TrpGly: 0.589 ± 0.166
0.353TrpHis: 0.353 ± 0.132
0.825TrpIle: 0.825 ± 0.208
0.766TrpLys: 0.766 ± 0.258
1.119TrpLeu: 1.119 ± 0.229
0.412TrpMet: 0.412 ± 0.142
1.178TrpAsn: 1.178 ± 0.309
0.059TrpPro: 0.059 ± 0.053
0.766TrpGln: 0.766 ± 0.226
0.53TrpArg: 0.53 ± 0.251
0.825TrpSer: 0.825 ± 0.333
1.06TrpThr: 1.06 ± 0.249
0.766TrpVal: 0.766 ± 0.213
0.236TrpTrp: 0.236 ± 0.136
0.353TrpTyr: 0.353 ± 0.139
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.239TyrAla: 2.239 ± 0.307
0.766TyrCys: 0.766 ± 0.242
2.887TyrAsp: 2.887 ± 0.525
2.887TyrGlu: 2.887 ± 0.487
1.237TyrPhe: 1.237 ± 0.342
2.592TyrGly: 2.592 ± 0.485
1.355TyrHis: 1.355 ± 0.31
2.474TyrIle: 2.474 ± 0.471
2.946TyrLys: 2.946 ± 0.563
4.124TyrLeu: 4.124 ± 0.493
0.707TyrMet: 0.707 ± 0.212
1.826TyrAsn: 1.826 ± 0.355
1.237TyrPro: 1.237 ± 0.269
1.885TyrGln: 1.885 ± 0.303
2.239TyrArg: 2.239 ± 0.421
2.062TyrSer: 2.062 ± 0.346
2.003TyrThr: 2.003 ± 0.423
1.885TyrVal: 1.885 ± 0.368
0.412TyrTrp: 0.412 ± 0.146
1.473TyrTyr: 1.473 ± 0.384
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (16976 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski