Amino acid dipepetide frequency for Enteroscipio rubneri

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.64AlaAla: 16.64 ± 0.26	2.386AlaCys: 2.386 ± 0.076	7.402AlaAsp: 7.402 ± 0.144	7.744AlaGlu: 7.744 ± 0.124	4.859AlaPhe: 4.859 ± 0.099	10.461AlaGly: 10.461 ± 0.222	2.054AlaHis: 2.054 ± 0.055	5.305AlaIle: 5.305 ± 0.095	4.071AlaLys: 4.071 ± 0.091	12.568AlaLeu: 12.568 ± 0.23	2.909AlaMet: 2.909 ± 0.069	3.108AlaAsn: 3.108 ± 0.168	4.783AlaPro: 4.783 ± 0.092	3.711AlaGln: 3.711 ± 0.08	7.547AlaArg: 7.547 ± 0.178	7.756AlaSer: 7.756 ± 0.162	5.844AlaThr: 5.844 ± 0.2	9.726AlaVal: 9.726 ± 0.151	1.405AlaTrp: 1.405 ± 0.065	3.118AlaTyr: 3.118 ± 0.087	0.0AlaXaa: 0.0 ± 0.0
Cys	1.874CysAla: 1.874 ± 0.055	0.248CysCys: 0.248 ± 0.018	1.005CysAsp: 1.005 ± 0.038	0.892CysGlu: 0.892 ± 0.038	0.613CysPhe: 0.613 ± 0.026	1.585CysGly: 1.585 ± 0.062	0.296CysHis: 0.296 ± 0.026	0.828CysIle: 0.828 ± 0.035	0.472CysLys: 0.472 ± 0.028	1.196CysLeu: 1.196 ± 0.042	0.391CysMet: 0.391 ± 0.025	0.355CysAsn: 0.355 ± 0.022	0.766CysPro: 0.766 ± 0.041	0.299CysGln: 0.299 ± 0.02	0.792CysArg: 0.792 ± 0.03	0.969CysSer: 0.969 ± 0.052	0.868CysThr: 0.868 ± 0.042	1.279CysVal: 1.279 ± 0.041	0.162CysTrp: 0.162 ± 0.016	0.486CysTyr: 0.486 ± 0.061	0.0CysXaa: 0.0 ± 0.0
Asp	8.288AspAla: 8.288 ± 0.131	0.691AspCys: 0.691 ± 0.031	4.065AspAsp: 4.065 ± 0.097	5.45AspGlu: 5.45 ± 0.11	2.305AspPhe: 2.305 ± 0.058	5.735AspGly: 5.735 ± 0.116	0.943AspHis: 0.943 ± 0.038	3.087AspIle: 3.087 ± 0.065	1.902AspLys: 1.902 ± 0.063	5.258AspLeu: 5.258 ± 0.075	1.506AspMet: 1.506 ± 0.043	1.42AspAsn: 1.42 ± 0.046	3.218AspPro: 3.218 ± 0.08	1.392AspGln: 1.392 ± 0.046	3.426AspArg: 3.426 ± 0.084	2.753AspSer: 2.753 ± 0.07	2.715AspThr: 2.715 ± 0.067	5.203AspVal: 5.203 ± 0.097	0.704AspTrp: 0.704 ± 0.048	1.798AspTyr: 1.798 ± 0.053	0.0AspXaa: 0.0 ± 0.0
Glu	8.202GluAla: 8.202 ± 0.118	0.66GluCys: 0.66 ± 0.03	3.771GluAsp: 3.771 ± 0.099	4.519GluGlu: 4.519 ± 0.123	2.001GluPhe: 2.001 ± 0.067	5.528GluGly: 5.528 ± 0.099	1.455GluHis: 1.455 ± 0.053	2.767GluIle: 2.767 ± 0.08	2.727GluLys: 2.727 ± 0.071	6.305GluLeu: 6.305 ± 0.125	1.506GluMet: 1.506 ± 0.043	2.069GluAsn: 2.069 ± 0.053	2.735GluPro: 2.735 ± 0.075	2.362GluGln: 2.362 ± 0.069	5.425GluArg: 5.425 ± 0.135	3.222GluSer: 3.222 ± 0.069	3.222GluThr: 3.222 ± 0.071	4.288GluVal: 4.288 ± 0.084	0.611GluTrp: 0.611 ± 0.04	1.689GluTyr: 1.689 ± 0.052	0.0GluXaa: 0.0 ± 0.0
Phe	4.985PheAla: 4.985 ± 0.095	0.663PheCys: 0.663 ± 0.031	2.979PheAsp: 2.979 ± 0.066	2.64PheGlu: 2.64 ± 0.067	1.584PhePhe: 1.584 ± 0.054	3.661PheGly: 3.661 ± 0.071	0.545PheHis: 0.545 ± 0.027	1.745PheIle: 1.745 ± 0.058	1.227PheLys: 1.227 ± 0.047	3.27PheLeu: 3.27 ± 0.095	0.986PheMet: 0.986 ± 0.038	1.12PheAsn: 1.12 ± 0.043	1.384PhePro: 1.384 ± 0.048	0.856PheGln: 0.856 ± 0.034	1.58PheArg: 1.58 ± 0.06	2.511PheSer: 2.511 ± 0.067	1.951PheThr: 1.951 ± 0.052	3.286PheVal: 3.286 ± 0.073	0.451PheTrp: 0.451 ± 0.023	1.121PheTyr: 1.121 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	9.854GlyAla: 9.854 ± 0.244	1.552GlyCys: 1.552 ± 0.053	4.645GlyAsp: 4.645 ± 0.081	4.95GlyGlu: 4.95 ± 0.086	3.628GlyPhe: 3.628 ± 0.087	7.07GlyGly: 7.07 ± 0.201	1.386GlyHis: 1.386 ± 0.044	4.826GlyIle: 4.826 ± 0.091	3.36GlyLys: 3.36 ± 0.085	7.539GlyLeu: 7.539 ± 0.113	2.523GlyMet: 2.523 ± 0.065	2.369GlyAsn: 2.369 ± 0.081	2.402GlyPro: 2.402 ± 0.064	2.148GlyGln: 2.148 ± 0.055	4.879GlyArg: 4.879 ± 0.1	5.812GlySer: 5.812 ± 0.232	5.09GlyThr: 5.09 ± 0.191	6.759GlyVal: 6.759 ± 0.101	0.989GlyTrp: 0.989 ± 0.037	2.857GlyTyr: 2.857 ± 0.086	0.0GlyXaa: 0.0 ± 0.0
His	2.137HisAla: 2.137 ± 0.059	0.276HisCys: 0.276 ± 0.019	1.156HisAsp: 1.156 ± 0.043	1.127HisGlu: 1.127 ± 0.044	0.623HisPhe: 0.623 ± 0.028	1.553HisGly: 1.553 ± 0.047	0.355HisHis: 0.355 ± 0.025	0.822HisIle: 0.822 ± 0.039	0.457HisLys: 0.457 ± 0.027	1.533HisLeu: 1.533 ± 0.056	0.44HisMet: 0.44 ± 0.023	0.416HisAsn: 0.416 ± 0.023	1.07HisPro: 1.07 ± 0.036	0.392HisGln: 0.392 ± 0.021	1.111HisArg: 1.111 ± 0.039	0.853HisSer: 0.853 ± 0.038	0.832HisThr: 0.832 ± 0.03	1.563HisVal: 1.563 ± 0.049	0.164HisTrp: 0.164 ± 0.015	0.483HisTyr: 0.483 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	6.542IleAla: 6.542 ± 0.108	0.735IleCys: 0.735 ± 0.033	3.673IleAsp: 3.673 ± 0.073	3.679IleGlu: 3.679 ± 0.072	1.265IlePhe: 1.265 ± 0.041	4.234IleGly: 4.234 ± 0.084	0.68IleHis: 0.68 ± 0.03	2.157IleIle: 2.157 ± 0.067	1.575IleLys: 1.575 ± 0.046	3.438IleLeu: 3.438 ± 0.105	1.326IleMet: 1.326 ± 0.045	1.351IleAsn: 1.351 ± 0.065	2.234IlePro: 2.234 ± 0.061	1.025IleGln: 1.025 ± 0.038	2.234IleArg: 2.234 ± 0.063	2.44IleSer: 2.44 ± 0.06	2.519IleThr: 2.519 ± 0.078	4.96IleVal: 4.96 ± 0.1	0.387IleTrp: 0.387 ± 0.023	1.05IleTyr: 1.05 ± 0.042	0.0IleXaa: 0.0 ± 0.0
Lys	3.881LysAla: 3.881 ± 0.078	0.357LysCys: 0.357 ± 0.025	2.014LysAsp: 2.014 ± 0.058	2.172LysGlu: 2.172 ± 0.067	0.885LysPhe: 0.885 ± 0.037	2.686LysGly: 2.686 ± 0.069	0.686LysHis: 0.686 ± 0.028	1.527LysIle: 1.527 ± 0.047	1.934LysLys: 1.934 ± 0.075	3.219LysLeu: 3.219 ± 0.073	0.849LysMet: 0.849 ± 0.037	1.306LysAsn: 1.306 ± 0.047	1.834LysPro: 1.834 ± 0.059	1.215LysGln: 1.215 ± 0.043	2.786LysArg: 2.786 ± 0.081	2.003LysSer: 2.003 ± 0.054	2.296LysThr: 2.296 ± 0.06	2.407LysVal: 2.407 ± 0.062	0.326LysTrp: 0.326 ± 0.024	0.93LysTyr: 0.93 ± 0.041	0.0LysXaa: 0.0 ± 0.0
Leu	12.643LeuAla: 12.643 ± 0.221	1.342LeuCys: 1.342 ± 0.042	6.023LeuAsp: 6.023 ± 0.098	5.529LeuGlu: 5.529 ± 0.107	3.841LeuPhe: 3.841 ± 0.086	7.384LeuGly: 7.384 ± 0.122	1.625LeuHis: 1.625 ± 0.046	3.969LeuIle: 3.969 ± 0.11	3.093LeuLys: 3.093 ± 0.072	8.466LeuLeu: 8.466 ± 0.192	2.427LeuMet: 2.427 ± 0.073	2.21LeuAsn: 2.21 ± 0.061	4.473LeuPro: 4.473 ± 0.105	1.989LeuGln: 1.989 ± 0.055	5.223LeuArg: 5.223 ± 0.117	5.889LeuSer: 5.889 ± 0.096	4.461LeuThr: 4.461 ± 0.086	8.483LeuVal: 8.483 ± 0.176	0.863LeuTrp: 0.863 ± 0.037	2.489LeuTyr: 2.489 ± 0.055	0.0LeuXaa: 0.0 ± 0.0
Met	2.929MetAla: 2.929 ± 0.061	0.319MetCys: 0.319 ± 0.02	1.448MetAsp: 1.448 ± 0.041	1.544MetGlu: 1.544 ± 0.05	0.785MetPhe: 0.785 ± 0.029	2.029MetGly: 2.029 ± 0.051	0.46MetHis: 0.46 ± 0.027	1.096MetIle: 1.096 ± 0.047	1.098MetLys: 1.098 ± 0.035	2.507MetLeu: 2.507 ± 0.077	0.72MetMet: 0.72 ± 0.036	0.917MetAsn: 0.917 ± 0.035	1.315MetPro: 1.315 ± 0.047	0.877MetGln: 0.877 ± 0.031	1.778MetArg: 1.778 ± 0.053	1.604MetSer: 1.604 ± 0.044	1.492MetThr: 1.492 ± 0.042	1.757MetVal: 1.757 ± 0.055	0.229MetTrp: 0.229 ± 0.019	0.548MetTyr: 0.548 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	3.461AsnAla: 3.461 ± 0.139	0.456AsnCys: 0.456 ± 0.082	1.696AsnAsp: 1.696 ± 0.055	1.737AsnGlu: 1.737 ± 0.054	0.842AsnPhe: 0.842 ± 0.034	2.46AsnGly: 2.46 ± 0.091	0.491AsnHis: 0.491 ± 0.024	1.405AsnIle: 1.405 ± 0.047	0.905AsnLys: 0.905 ± 0.036	2.659AsnLeu: 2.659 ± 0.06	0.763AsnMet: 0.763 ± 0.032	0.817AsnAsn: 0.817 ± 0.066	1.797AsnPro: 1.797 ± 0.053	0.755AsnGln: 0.755 ± 0.046	1.665AsnArg: 1.665 ± 0.047	1.39AsnSer: 1.39 ± 0.074	1.539AsnThr: 1.539 ± 0.129	2.336AsnVal: 2.336 ± 0.074	0.299AsnTrp: 0.299 ± 0.023	0.804AsnTyr: 0.804 ± 0.042	0.0AsnXaa: 0.0 ± 0.0
Pro	5.182ProAla: 5.182 ± 0.112	0.613ProCys: 0.613 ± 0.024	3.056ProAsp: 3.056 ± 0.069	3.357ProGlu: 3.357 ± 0.07	1.849ProPhe: 1.849 ± 0.049	3.455ProGly: 3.455 ± 0.065	0.859ProHis: 0.859 ± 0.034	2.03ProIle: 2.03 ± 0.054	1.41ProLys: 1.41 ± 0.046	4.049ProLeu: 4.049 ± 0.093	0.904ProMet: 0.904 ± 0.034	1.198ProAsn: 1.198 ± 0.038	1.455ProPro: 1.455 ± 0.058	1.347ProGln: 1.347 ± 0.053	2.257ProArg: 2.257 ± 0.064	2.898ProSer: 2.898 ± 0.067	2.346ProThr: 2.346 ± 0.055	3.653ProVal: 3.653 ± 0.072	0.484ProTrp: 0.484 ± 0.025	1.394ProTyr: 1.394 ± 0.051	0.0ProXaa: 0.0 ± 0.0
Gln	3.461GlnAla: 3.461 ± 0.07	0.314GlnCys: 0.314 ± 0.02	1.39GlnAsp: 1.39 ± 0.047	1.689GlnGlu: 1.689 ± 0.055	0.877GlnPhe: 0.877 ± 0.035	2.262GlnGly: 2.262 ± 0.059	0.531GlnHis: 0.531 ± 0.024	1.341GlnIle: 1.341 ± 0.044	1.118GlnLys: 1.118 ± 0.038	2.543GlnLeu: 2.543 ± 0.052	0.712GlnMet: 0.712 ± 0.029	0.903GlnAsn: 0.903 ± 0.04	1.313GlnPro: 1.313 ± 0.052	1.006GlnGln: 1.006 ± 0.043	1.966GlnArg: 1.966 ± 0.061	1.45GlnSer: 1.45 ± 0.049	1.538GlnThr: 1.538 ± 0.047	2.107GlnVal: 2.107 ± 0.063	0.278GlnTrp: 0.278 ± 0.021	0.7GlnTyr: 0.7 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	7.183ArgAla: 7.183 ± 0.169	0.975ArgCys: 0.975 ± 0.043	3.311ArgAsp: 3.311 ± 0.072	4.183ArgGlu: 4.183 ± 0.111	2.645ArgPhe: 2.645 ± 0.075	4.228ArgGly: 4.228 ± 0.093	1.1ArgHis: 1.1 ± 0.044	3.458ArgIle: 3.458 ± 0.087	2.265ArgLys: 2.265 ± 0.074	5.422ArgLeu: 5.422 ± 0.127	1.954ArgMet: 1.954 ± 0.061	1.675ArgAsn: 1.675 ± 0.049	2.365ArgPro: 2.365 ± 0.073	1.742ArgGln: 1.742 ± 0.054	4.558ArgArg: 4.558 ± 0.129	3.435ArgSer: 3.435 ± 0.081	3.391ArgThr: 3.391 ± 0.071	4.37ArgVal: 4.37 ± 0.082	0.633ArgTrp: 0.633 ± 0.031	1.818ArgTyr: 1.818 ± 0.055	0.0ArgXaa: 0.0 ± 0.0
Ser	6.524SerAla: 6.524 ± 0.14	0.861SerCys: 0.861 ± 0.039	3.46SerAsp: 3.46 ± 0.068	3.381SerGlu: 3.381 ± 0.079	2.715SerPhe: 2.715 ± 0.055	5.492SerGly: 5.492 ± 0.197	0.97SerHis: 0.97 ± 0.036	3.177SerIle: 3.177 ± 0.077	2.137SerLys: 2.137 ± 0.058	5.478SerLeu: 5.478 ± 0.101	1.588SerMet: 1.588 ± 0.051	1.761SerAsn: 1.761 ± 0.106	2.346SerPro: 2.346 ± 0.055	1.654SerGln: 1.654 ± 0.05	3.127SerArg: 3.127 ± 0.072	4.112SerSer: 4.112 ± 0.109	3.219SerThr: 3.219 ± 0.161	4.729SerVal: 4.729 ± 0.102	0.765SerTrp: 0.765 ± 0.038	1.819SerTyr: 1.819 ± 0.068	0.0SerXaa: 0.0 ± 0.0
Thr	5.868ThrAla: 5.868 ± 0.18	0.821ThrCys: 0.821 ± 0.034	2.898ThrAsp: 2.898 ± 0.086	2.63ThrGlu: 2.63 ± 0.066	2.259ThrPhe: 2.259 ± 0.064	5.141ThrGly: 5.141 ± 0.205	0.827ThrHis: 0.827 ± 0.034	2.694ThrIle: 2.694 ± 0.064	1.648ThrLys: 1.648 ± 0.059	4.996ThrLeu: 4.996 ± 0.099	1.215ThrMet: 1.215 ± 0.036	1.542ThrAsn: 1.542 ± 0.11	2.744ThrPro: 2.744 ± 0.12	1.253ThrGln: 1.253 ± 0.043	2.557ThrArg: 2.557 ± 0.07	3.083ThrSer: 3.083 ± 0.117	3.037ThrThr: 3.037 ± 0.144	4.869ThrVal: 4.869 ± 0.102	0.695ThrTrp: 0.695 ± 0.04	1.689ThrTyr: 1.689 ± 0.076	0.0ThrXaa: 0.0 ± 0.0
Val	9.44ValAla: 9.44 ± 0.132	1.466ValCys: 1.466 ± 0.055	5.181ValAsp: 5.181 ± 0.088	5.356ValGlu: 5.356 ± 0.104	3.354ValPhe: 3.354 ± 0.088	6.334ValGly: 6.334 ± 0.166	1.406ValHis: 1.406 ± 0.037	3.573ValIle: 3.573 ± 0.08	2.74ValLys: 2.74 ± 0.066	8.075ValLeu: 8.075 ± 0.161	1.874ValMet: 1.874 ± 0.062	2.41ValAsn: 2.41 ± 0.076	3.913ValPro: 3.913 ± 0.074	2.245ValGln: 2.245 ± 0.061	5.142ValArg: 5.142 ± 0.098	5.145ValSer: 5.145 ± 0.096	4.07ValThr: 4.07 ± 0.118	7.324ValVal: 7.324 ± 0.139	0.847ValTrp: 0.847 ± 0.033	2.202ValTyr: 2.202 ± 0.057	0.0ValXaa: 0.0 ± 0.0
Trp	1.082TrpAla: 1.082 ± 0.037	0.177TrpCys: 0.177 ± 0.015	0.65TrpAsp: 0.65 ± 0.03	0.65TrpGlu: 0.65 ± 0.031	0.477TrpPhe: 0.477 ± 0.022	0.885TrpGly: 0.885 ± 0.04	0.209TrpHis: 0.209 ± 0.016	0.635TrpIle: 0.635 ± 0.031	0.386TrpLys: 0.386 ± 0.024	1.045TrpLeu: 1.045 ± 0.048	0.339TrpMet: 0.339 ± 0.02	0.374TrpAsn: 0.374 ± 0.019	0.377TrpPro: 0.377 ± 0.022	0.367TrpGln: 0.367 ± 0.023	0.674TrpArg: 0.674 ± 0.033	0.578TrpSer: 0.578 ± 0.029	0.568TrpThr: 0.568 ± 0.03	0.743TrpVal: 0.743 ± 0.05	0.172TrpTrp: 0.172 ± 0.017	0.319TrpTyr: 0.319 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.224TyrAla: 3.224 ± 0.1	0.465TyrCys: 0.465 ± 0.024	2.036TyrAsp: 2.036 ± 0.05	1.833TyrGlu: 1.833 ± 0.062	1.138TyrPhe: 1.138 ± 0.043	2.456TyrGly: 2.456 ± 0.06	0.486TyrHis: 0.486 ± 0.026	1.064TyrIle: 1.064 ± 0.047	0.79TyrLys: 0.79 ± 0.047	2.857TyrLeu: 2.857 ± 0.066	0.517TyrMet: 0.517 ± 0.024	0.958TyrAsn: 0.958 ± 0.08	1.272TyrPro: 1.272 ± 0.047	0.861TyrGln: 0.861 ± 0.041	1.985TyrArg: 1.985 ± 0.06	1.413TyrSer: 1.413 ± 0.055	1.466TyrThr: 1.466 ± 0.059	2.252TyrVal: 2.252 ± 0.075	0.244TyrTrp: 0.244 ± 0.017	0.885TyrTyr: 0.885 ± 0.041	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2260 proteins (802988 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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