Streptococcus phage Javan166

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AR73|A0A4D6AR73_9CAUD DNA helicase OS=Streptococcus phage Javan166 OX=2548017 GN=Javan166_0054 PE=4 SV=1
MM1 pKa = 7.04NWKK4 pKa = 9.33HH5 pKa = 6.23CYY7 pKa = 9.19LRR9 pKa = 11.84KK10 pKa = 7.15TTEE13 pKa = 3.79EE14 pKa = 4.0EE15 pKa = 4.06KK16 pKa = 11.23VEE18 pKa = 4.54FSCDD22 pKa = 3.77EE23 pKa = 4.05VWDD26 pKa = 4.26GQIPEE31 pKa = 4.77LDD33 pKa = 3.75GDD35 pKa = 3.96VLIYY39 pKa = 10.93QNDD42 pKa = 4.18HH43 pKa = 6.46YY44 pKa = 11.55DD45 pKa = 3.68VDD47 pKa = 4.17CMTDD51 pKa = 2.79VDD53 pKa = 4.27TGIALYY59 pKa = 11.11NNDD62 pKa = 3.3YY63 pKa = 11.54DD64 pKa = 4.35DD65 pKa = 6.1FYY67 pKa = 10.37WCEE70 pKa = 3.79IEE72 pKa = 6.14APDD75 pKa = 4.42IGNGRR80 pKa = 11.84KK81 pKa = 8.96RR82 pKa = 3.32

Molecular weight: 9.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6ASN7|A0A4D6ASN7_9CAUD Single stranded DNA-binding protein OS=Streptococcus phage Javan166 OX=2548017 GN=Javan166_0053 PE=4 SV=1
MM1 pKa = 7.33SRR3 pKa = 11.84DD4 pKa = 3.1KK5 pKa = 11.1TRR7 pKa = 11.84IRR9 pKa = 11.84SIVKK13 pKa = 9.63FMKK16 pKa = 10.41QLGASEE22 pKa = 4.14DD23 pKa = 3.47RR24 pKa = 11.84ARR26 pKa = 11.84QTKK29 pKa = 10.13RR30 pKa = 11.84IAEE33 pKa = 4.08LTGNGGG39 pKa = 3.26

Molecular weight: 4.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

13344

37

1211

190.6

21.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.797 ± 0.914

0.607 ± 0.133

6.7 ± 0.361

7.584 ± 0.561

4.242 ± 0.263

6.595 ± 0.551

1.311 ± 0.142

6.85 ± 0.217

9.09 ± 0.476

8.596 ± 0.33

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.51 ± 0.183

5.306 ± 0.318

2.63 ± 0.24

4.197 ± 0.23

4.249 ± 0.286

5.95 ± 0.453

6.033 ± 0.285

6.063 ± 0.31

1.079 ± 0.136

3.612 ± 0.325
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski