Polyporus arcularius HHB13444

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Polyporales; Polyporaceae; Polyporus; Polyporus arcularius

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17473 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C3PB19|A0A5C3PB19_9APHY Peroxidase OS=Polyporus arcularius HHB13444 OX=1314778 GN=K466DRAFT_549730 PE=3 SV=1
MM1 pKa = 7.37LTPHH5 pKa = 7.56RR6 pKa = 11.84WPSSCVSGHH15 pKa = 4.72VSAVRR20 pKa = 11.84GSLGIYY26 pKa = 9.66NPLRR30 pKa = 11.84EE31 pKa = 4.05RR32 pKa = 11.84FISNHH37 pKa = 5.6RR38 pKa = 11.84ASGSSISTSSSQASNQTLSVSSITSSANAPDD69 pKa = 4.46APSSTISIVSSTDD82 pKa = 2.99LATSVVATDD91 pKa = 3.68SADD94 pKa = 3.58PTDD97 pKa = 3.79SSVITISTATDD108 pKa = 4.16FFNGTATEE116 pKa = 4.4SSSPTATDD124 pKa = 3.23TTTDD128 pKa = 2.98ASATDD133 pKa = 3.97VSALPTSTDD142 pKa = 3.41DD143 pKa = 5.03ASATDD148 pKa = 3.57SSSPSTATDD157 pKa = 3.37SDD159 pKa = 4.07PGTSVSTDD167 pKa = 2.81AAAPTGTGISFDD179 pKa = 4.51LPTNIPPACLALASGTVISTPSLTSAAASATDD211 pKa = 3.8SSAADD216 pKa = 3.71PSSTALSATDD226 pKa = 3.86ASATEE231 pKa = 4.52PATADD236 pKa = 3.52PSATDD241 pKa = 3.63SLNSTSIVVTSSIPSSSISSSAAAVNVTATAIATVTDD278 pKa = 4.05VSSSAATASPTDD290 pKa = 3.28GSTNSTISRR299 pKa = 11.84RR300 pKa = 11.84IAQTDD305 pKa = 4.0LPDD308 pKa = 4.22VAQSWQDD315 pKa = 3.23LCLVSGGDD323 pKa = 3.18IFTHH327 pKa = 6.05EE328 pKa = 4.41PCVTLAGVDD337 pKa = 4.45GINALLGDD345 pKa = 5.2ADD347 pKa = 4.17PCAQQDD353 pKa = 3.75NADD356 pKa = 3.63AMIDD360 pKa = 3.8FAKK363 pKa = 10.85SPGVTNADD371 pKa = 3.34ALIANAIAYY380 pKa = 7.5RR381 pKa = 11.84QHH383 pKa = 6.39PRR385 pKa = 11.84NAINVNGVVPATPYY399 pKa = 9.81CQRR402 pKa = 11.84APRR405 pKa = 11.84NAEE408 pKa = 3.48LQGIVNTQLDD418 pKa = 4.11GVNAGIYY425 pKa = 10.49GSVNLGLYY433 pKa = 10.61AFGAAGTCPFGQSPDD448 pKa = 3.34VSTCICSS455 pKa = 3.13

Molecular weight: 45.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C3PZ71|A0A5C3PZ71_9APHY NAD(P)-binding protein OS=Polyporus arcularius HHB13444 OX=1314778 GN=K466DRAFT_596096 PE=3 SV=1
MM1 pKa = 7.82AEE3 pKa = 4.32LSVLTARR10 pKa = 11.84PSSSSRR16 pKa = 11.84RR17 pKa = 11.84GRR19 pKa = 11.84PRR21 pKa = 11.84RR22 pKa = 11.84LPKK25 pKa = 10.19QVGAPLHH32 pKa = 6.12RR33 pKa = 11.84ASHH36 pKa = 5.65PRR38 pKa = 11.84PRR40 pKa = 11.84ALRR43 pKa = 11.84LPVRR47 pKa = 11.84HH48 pKa = 6.16LRR50 pKa = 11.84RR51 pKa = 11.84GRR53 pKa = 11.84TPPRR57 pKa = 11.84PQLTPRR63 pKa = 11.84WPRR66 pKa = 11.84HH67 pKa = 5.05IPPPLLLPLRR77 pKa = 11.84HH78 pKa = 5.76RR79 pKa = 11.84QRR81 pKa = 11.84MQHH84 pKa = 4.37HH85 pKa = 5.67HH86 pKa = 6.09HH87 pKa = 6.09RR88 pKa = 11.84RR89 pKa = 11.84SRR91 pKa = 11.84RR92 pKa = 11.84VMWQTCSRR100 pKa = 11.84AALRR104 pKa = 11.84RR105 pKa = 11.84TGHH108 pKa = 4.55TTMCRR113 pKa = 11.84GYY115 pKa = 10.54LGSLVSTLRR124 pKa = 11.84HH125 pKa = 6.08LPPSLWLSPRR135 pKa = 11.84PHH137 pKa = 7.8HH138 pKa = 6.7GMQHH142 pKa = 4.82TRR144 pKa = 11.84HH145 pKa = 5.92PRR147 pKa = 11.84LRR149 pKa = 11.84LPRR152 pKa = 11.84LQRR155 pKa = 11.84RR156 pKa = 11.84RR157 pKa = 11.84TGKK160 pKa = 8.99TSVCSSGTRR169 pKa = 11.84SGLSKK174 pKa = 10.47LLKK177 pKa = 9.65PRR179 pKa = 11.84RR180 pKa = 11.84PRR182 pKa = 3.63

Molecular weight: 21.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17466

7

17473

6874114

49

5043

393.4

43.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.427 ± 0.018

1.397 ± 0.009

5.675 ± 0.012

5.784 ± 0.017

3.557 ± 0.014

6.534 ± 0.02

2.692 ± 0.009

4.291 ± 0.012

3.969 ± 0.017

9.244 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.057 ± 0.007

2.909 ± 0.01

6.9 ± 0.025

3.57 ± 0.01

6.907 ± 0.019

8.324 ± 0.024

5.967 ± 0.012

6.617 ± 0.012

1.519 ± 0.008

2.662 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski