Amino acid dipepetide frequency for Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.797AlaAla: 13.797 ± 0.212	0.958AlaCys: 0.958 ± 0.038	6.24AlaAsp: 6.24 ± 0.095	6.851AlaGlu: 6.851 ± 0.137	3.463AlaPhe: 3.463 ± 0.071	8.949AlaGly: 8.949 ± 0.144	2.764AlaHis: 2.764 ± 0.068	6.029AlaIle: 6.029 ± 0.106	3.776AlaLys: 3.776 ± 0.088	11.102AlaLeu: 11.102 ± 0.139	2.773AlaMet: 2.773 ± 0.063	2.713AlaAsn: 2.713 ± 0.066	4.685AlaPro: 4.685 ± 0.095	4.701AlaGln: 4.701 ± 0.105	6.272AlaArg: 6.272 ± 0.1	5.948AlaSer: 5.948 ± 0.089	6.837AlaThr: 6.837 ± 0.1	9.091AlaVal: 9.091 ± 0.113	1.563AlaTrp: 1.563 ± 0.049	2.24AlaTyr: 2.24 ± 0.059	0.0AlaXaa: 0.0 ± 0.0
Cys	0.987CysAla: 0.987 ± 0.032	0.138CysCys: 0.138 ± 0.014	0.576CysAsp: 0.576 ± 0.032	0.476CysGlu: 0.476 ± 0.031	0.312CysPhe: 0.312 ± 0.022	0.878CysGly: 0.878 ± 0.033	0.232CysHis: 0.232 ± 0.018	0.408CysIle: 0.408 ± 0.024	0.171CysLys: 0.171 ± 0.016	0.658CysLeu: 0.658 ± 0.028	0.182CysMet: 0.182 ± 0.014	0.221CysAsn: 0.221 ± 0.02	0.396CysPro: 0.396 ± 0.025	0.227CysGln: 0.227 ± 0.017	0.405CysArg: 0.405 ± 0.026	0.625CysSer: 0.625 ± 0.027	0.545CysThr: 0.545 ± 0.031	0.756CysVal: 0.756 ± 0.03	0.114CysTrp: 0.114 ± 0.011	0.176CysTyr: 0.176 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	6.539AspAla: 6.539 ± 0.12	0.483AspCys: 0.483 ± 0.025	3.434AspAsp: 3.434 ± 0.082	3.547AspGlu: 3.547 ± 0.074	2.018AspPhe: 2.018 ± 0.062	4.514AspGly: 4.514 ± 0.09	1.539AspHis: 1.539 ± 0.048	3.131AspIle: 3.131 ± 0.059	2.067AspLys: 2.067 ± 0.058	5.44AspLeu: 5.44 ± 0.084	1.267AspMet: 1.267 ± 0.043	1.836AspAsn: 1.836 ± 0.054	3.535AspPro: 3.535 ± 0.081	1.839AspGln: 1.839 ± 0.058	3.364AspArg: 3.364 ± 0.066	3.611AspSer: 3.611 ± 0.073	3.233AspThr: 3.233 ± 0.073	4.923AspVal: 4.923 ± 0.089	0.756AspTrp: 0.756 ± 0.036	1.466AspTyr: 1.466 ± 0.048	0.0AspXaa: 0.0 ± 0.0
Glu	6.128GluAla: 6.128 ± 0.118	0.434GluCys: 0.434 ± 0.025	3.0GluAsp: 3.0 ± 0.074	3.764GluGlu: 3.764 ± 0.094	2.021GluPhe: 2.021 ± 0.057	3.913GluGly: 3.913 ± 0.074	1.61GluHis: 1.61 ± 0.045	3.627GluIle: 3.627 ± 0.066	2.837GluLys: 2.837 ± 0.085	6.469GluLeu: 6.469 ± 0.112	1.224GluMet: 1.224 ± 0.047	2.031GluAsn: 2.031 ± 0.048	2.439GluPro: 2.439 ± 0.07	2.661GluGln: 2.661 ± 0.063	3.502GluArg: 3.502 ± 0.075	3.414GluSer: 3.414 ± 0.073	3.007GluThr: 3.007 ± 0.071	4.703GluVal: 4.703 ± 0.094	0.7GluTrp: 0.7 ± 0.033	1.362GluTyr: 1.362 ± 0.045	0.0GluXaa: 0.0 ± 0.0
Phe	3.723PheAla: 3.723 ± 0.073	0.273PheCys: 0.273 ± 0.019	2.52PheAsp: 2.52 ± 0.058	1.846PheGlu: 1.846 ± 0.054	1.297PhePhe: 1.297 ± 0.045	3.269PheGly: 3.269 ± 0.072	0.808PheHis: 0.808 ± 0.038	1.87PheIle: 1.87 ± 0.057	1.008PheLys: 1.008 ± 0.048	2.893PheLeu: 2.893 ± 0.066	0.644PheMet: 0.644 ± 0.032	1.133PheAsn: 1.133 ± 0.042	1.526PhePro: 1.526 ± 0.05	0.902PheGln: 0.902 ± 0.036	1.603PheArg: 1.603 ± 0.048	2.318PheSer: 2.318 ± 0.057	2.054PheThr: 2.054 ± 0.066	2.698PheVal: 2.698 ± 0.064	0.432PheTrp: 0.432 ± 0.024	0.847PheTyr: 0.847 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	7.895GlyAla: 7.895 ± 0.118	0.746GlyCys: 0.746 ± 0.034	4.275GlyAsp: 4.275 ± 0.084	4.411GlyGlu: 4.411 ± 0.081	3.261GlyPhe: 3.261 ± 0.067	6.042GlyGly: 6.042 ± 0.128	1.954GlyHis: 1.954 ± 0.053	5.002GlyIle: 5.002 ± 0.091	3.361GlyLys: 3.361 ± 0.071	7.475GlyLeu: 7.475 ± 0.107	2.129GlyMet: 2.129 ± 0.054	2.384GlyAsn: 2.384 ± 0.061	2.877GlyPro: 2.877 ± 0.07	2.611GlyGln: 2.611 ± 0.058	4.482GlyArg: 4.482 ± 0.093	4.93GlySer: 4.93 ± 0.088	4.961GlyThr: 4.961 ± 0.094	6.793GlyVal: 6.793 ± 0.114	1.295GlyTrp: 1.295 ± 0.042	2.144GlyTyr: 2.144 ± 0.057	0.0GlyXaa: 0.0 ± 0.0
His	2.422HisAla: 2.422 ± 0.057	0.252HisCys: 0.252 ± 0.022	1.518HisAsp: 1.518 ± 0.046	1.326HisGlu: 1.326 ± 0.042	0.684HisPhe: 0.684 ± 0.033	2.023HisGly: 2.023 ± 0.056	0.75HisHis: 0.75 ± 0.037	1.297HisIle: 1.297 ± 0.041	0.724HisLys: 0.724 ± 0.034	2.114HisLeu: 2.114 ± 0.06	0.527HisMet: 0.527 ± 0.026	0.834HisAsn: 0.834 ± 0.034	1.456HisPro: 1.456 ± 0.046	0.767HisGln: 0.767 ± 0.034	1.581HisArg: 1.581 ± 0.048	1.486HisSer: 1.486 ± 0.051	1.45HisThr: 1.45 ± 0.044	1.798HisVal: 1.798 ± 0.054	0.338HisTrp: 0.338 ± 0.023	0.648HisTyr: 0.648 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	7.22IleAla: 7.22 ± 0.121	0.484IleCys: 0.484 ± 0.024	4.204IleAsp: 4.204 ± 0.088	3.317IleGlu: 3.317 ± 0.073	1.714IlePhe: 1.714 ± 0.053	4.561IleGly: 4.561 ± 0.102	1.233IleHis: 1.233 ± 0.044	2.971IleIle: 2.971 ± 0.078	1.883IleLys: 1.883 ± 0.054	4.386IleLeu: 4.386 ± 0.085	1.106IleMet: 1.106 ± 0.04	1.885IleAsn: 1.885 ± 0.055	3.017IlePro: 3.017 ± 0.062	1.432IleGln: 1.432 ± 0.048	2.922IleArg: 2.922 ± 0.063	3.525IleSer: 3.525 ± 0.074	3.774IleThr: 3.774 ± 0.078	4.903IleVal: 4.903 ± 0.096	0.56IleTrp: 0.56 ± 0.031	1.124IleTyr: 1.124 ± 0.037	0.0IleXaa: 0.0 ± 0.0
Lys	3.841LysAla: 3.841 ± 0.096	0.2LysCys: 0.2 ± 0.019	2.136LysAsp: 2.136 ± 0.07	2.337LysGlu: 2.337 ± 0.072	1.0LysPhe: 1.0 ± 0.04	2.45LysGly: 2.45 ± 0.074	0.758LysHis: 0.758 ± 0.026	2.115LysIle: 2.115 ± 0.067	2.198LysLys: 2.198 ± 0.079	3.247LysLeu: 3.247 ± 0.081	0.881LysMet: 0.881 ± 0.038	1.614LysAsn: 1.614 ± 0.055	1.842LysPro: 1.842 ± 0.06	1.333LysGln: 1.333 ± 0.043	2.311LysArg: 2.311 ± 0.061	2.046LysSer: 2.046 ± 0.057	2.224LysThr: 2.224 ± 0.06	2.898LysVal: 2.898 ± 0.068	0.43LysTrp: 0.43 ± 0.024	0.808LysTyr: 0.808 ± 0.037	0.0LysXaa: 0.0 ± 0.0
Leu	11.06LeuAla: 11.06 ± 0.133	0.914LeuCys: 0.914 ± 0.038	5.664LeuAsp: 5.664 ± 0.096	4.968LeuGlu: 4.968 ± 0.101	2.955LeuPhe: 2.955 ± 0.079	8.122LeuGly: 8.122 ± 0.113	1.988LeuHis: 1.988 ± 0.053	5.321LeuIle: 5.321 ± 0.106	3.109LeuLys: 3.109 ± 0.079	8.482LeuLeu: 8.482 ± 0.157	2.133LeuMet: 2.133 ± 0.055	2.837LeuAsn: 2.837 ± 0.066	4.952LeuPro: 4.952 ± 0.09	2.731LeuGln: 2.731 ± 0.066	6.082LeuArg: 6.082 ± 0.092	6.36LeuSer: 6.36 ± 0.103	5.712LeuThr: 5.712 ± 0.074	7.496LeuVal: 7.496 ± 0.116	1.175LeuTrp: 1.175 ± 0.046	1.823LeuTyr: 1.823 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	2.526MetAla: 2.526 ± 0.057	0.205MetCys: 0.205 ± 0.016	1.102MetAsp: 1.102 ± 0.043	0.976MetGlu: 0.976 ± 0.038	0.776MetPhe: 0.776 ± 0.034	1.758MetGly: 1.758 ± 0.05	0.502MetHis: 0.502 ± 0.026	1.317MetIle: 1.317 ± 0.042	0.869MetLys: 0.869 ± 0.032	2.293MetLeu: 2.293 ± 0.06	0.582MetMet: 0.582 ± 0.03	0.842MetAsn: 0.842 ± 0.034	1.184MetPro: 1.184 ± 0.043	0.749MetGln: 0.749 ± 0.03	1.568MetArg: 1.568 ± 0.044	1.831MetSer: 1.831 ± 0.05	1.602MetThr: 1.602 ± 0.049	1.756MetVal: 1.756 ± 0.05	0.332MetTrp: 0.332 ± 0.021	0.407MetTyr: 0.407 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	3.259AsnAla: 3.259 ± 0.068	0.252AsnCys: 0.252 ± 0.017	1.839AsnAsp: 1.839 ± 0.061	1.677AsnGlu: 1.677 ± 0.053	0.998AsnPhe: 0.998 ± 0.042	2.508AsnGly: 2.508 ± 0.068	0.688AsnHis: 0.688 ± 0.029	1.768AsnIle: 1.768 ± 0.05	1.251AsnLys: 1.251 ± 0.042	2.708AsnLeu: 2.708 ± 0.059	0.656AsnMet: 0.656 ± 0.03	1.198AsnAsn: 1.198 ± 0.041	2.12AsnPro: 2.12 ± 0.061	1.041AsnGln: 1.041 ± 0.038	1.74AsnArg: 1.74 ± 0.049	1.894AsnSer: 1.894 ± 0.049	1.799AsnThr: 1.799 ± 0.055	2.369AsnVal: 2.369 ± 0.059	0.392AsnTrp: 0.392 ± 0.022	0.789AsnTyr: 0.789 ± 0.036	0.0AsnXaa: 0.0 ± 0.0
Pro	5.265ProAla: 5.265 ± 0.094	0.288ProCys: 0.288 ± 0.022	2.97ProAsp: 2.97 ± 0.064	3.934ProGlu: 3.934 ± 0.083	1.57ProPhe: 1.57 ± 0.05	3.643ProGly: 3.643 ± 0.069	1.322ProHis: 1.322 ± 0.05	2.37ProIle: 2.37 ± 0.059	1.717ProLys: 1.717 ± 0.051	4.242ProLeu: 4.242 ± 0.081	1.069ProMet: 1.069 ± 0.037	1.535ProAsn: 1.535 ± 0.048	1.754ProPro: 1.754 ± 0.062	2.098ProGln: 2.098 ± 0.053	2.651ProArg: 2.651 ± 0.057	3.041ProSer: 3.041 ± 0.08	3.217ProThr: 3.217 ± 0.078	4.191ProVal: 4.191 ± 0.076	0.758ProTrp: 0.758 ± 0.036	1.086ProTyr: 1.086 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	3.916GlnAla: 3.916 ± 0.089	0.291GlnCys: 0.291 ± 0.019	1.437GlnAsp: 1.437 ± 0.046	1.98GlnGlu: 1.98 ± 0.057	1.077GlnPhe: 1.077 ± 0.042	2.45GlnGly: 2.45 ± 0.058	0.92GlnHis: 0.92 ± 0.037	1.803GlnIle: 1.803 ± 0.051	1.296GlnLys: 1.296 ± 0.045	3.934GlnLeu: 3.934 ± 0.084	0.804GlnMet: 0.804 ± 0.035	0.844GlnAsn: 0.844 ± 0.035	1.856GlnPro: 1.856 ± 0.061	1.759GlnGln: 1.759 ± 0.053	2.966GlnArg: 2.966 ± 0.062	1.948GlnSer: 1.948 ± 0.064	1.588GlnThr: 1.588 ± 0.051	2.515GlnVal: 2.515 ± 0.061	0.714GlnTrp: 0.714 ± 0.036	0.72GlnTyr: 0.72 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	5.719ArgAla: 5.719 ± 0.092	0.507ArgCys: 0.507 ± 0.027	3.39ArgAsp: 3.39 ± 0.07	3.765ArgGlu: 3.765 ± 0.081	2.107ArgPhe: 2.107 ± 0.057	4.28ArgGly: 4.28 ± 0.085	1.388ArgHis: 1.388 ± 0.042	3.556ArgIle: 3.556 ± 0.084	2.235ArgLys: 2.235 ± 0.06	5.489ArgLeu: 5.489 ± 0.096	1.586ArgMet: 1.586 ± 0.049	2.003ArgAsn: 2.003 ± 0.057	2.606ArgPro: 2.606 ± 0.057	2.05ArgGln: 2.05 ± 0.049	4.631ArgArg: 4.631 ± 0.109	3.61ArgSer: 3.61 ± 0.069	3.433ArgThr: 3.433 ± 0.07	4.408ArgVal: 4.408 ± 0.078	0.975ArgTrp: 0.975 ± 0.04	1.697ArgTyr: 1.697 ± 0.057	0.0ArgXaa: 0.0 ± 0.0
Ser	6.579SerAla: 6.579 ± 0.104	0.477SerCys: 0.477 ± 0.024	3.545SerAsp: 3.545 ± 0.072	3.525SerGlu: 3.525 ± 0.088	2.189SerPhe: 2.189 ± 0.059	5.395SerGly: 5.395 ± 0.1	1.474SerHis: 1.474 ± 0.043	3.299SerIle: 3.299 ± 0.078	2.12SerLys: 2.12 ± 0.054	5.799SerLeu: 5.799 ± 0.111	1.663SerMet: 1.663 ± 0.05	1.83SerAsn: 1.83 ± 0.063	3.003SerPro: 3.003 ± 0.068	2.258SerGln: 2.258 ± 0.053	3.4SerArg: 3.4 ± 0.068	4.186SerSer: 4.186 ± 0.09	4.058SerThr: 4.058 ± 0.085	5.086SerVal: 5.086 ± 0.097	0.929SerTrp: 0.929 ± 0.036	1.535SerTyr: 1.535 ± 0.05	0.0SerXaa: 0.0 ± 0.0
Thr	6.463ThrAla: 6.463 ± 0.108	0.517ThrCys: 0.517 ± 0.028	3.324ThrAsp: 3.324 ± 0.068	3.233ThrGlu: 3.233 ± 0.068	2.024ThrPhe: 2.024 ± 0.058	4.955ThrGly: 4.955 ± 0.071	1.478ThrHis: 1.478 ± 0.037	3.564ThrIle: 3.564 ± 0.076	2.165ThrLys: 2.165 ± 0.059	5.764ThrLeu: 5.764 ± 0.092	1.379ThrMet: 1.379 ± 0.048	1.781ThrAsn: 1.781 ± 0.055	3.778ThrPro: 3.778 ± 0.078	2.115ThrGln: 2.115 ± 0.062	2.944ThrArg: 2.944 ± 0.066	3.822ThrSer: 3.822 ± 0.08	4.246ThrThr: 4.246 ± 0.086	5.278ThrVal: 5.278 ± 0.089	0.898ThrTrp: 0.898 ± 0.039	1.399ThrTyr: 1.399 ± 0.044	0.0ThrXaa: 0.0 ± 0.0
Val	9.679ValAla: 9.679 ± 0.138	0.717ValCys: 0.717 ± 0.029	5.323ValAsp: 5.323 ± 0.086	4.962ValGlu: 4.962 ± 0.099	2.738ValPhe: 2.738 ± 0.066	6.284ValGly: 6.284 ± 0.103	1.719ValHis: 1.719 ± 0.047	4.848ValIle: 4.848 ± 0.094	2.6ValLys: 2.6 ± 0.066	7.586ValLeu: 7.586 ± 0.122	1.813ValMet: 1.813 ± 0.055	2.283ValAsn: 2.283 ± 0.059	3.925ValPro: 3.925 ± 0.079	2.09ValGln: 2.09 ± 0.051	4.588ValArg: 4.588 ± 0.082	5.286ValSer: 5.286 ± 0.099	5.333ValThr: 5.333 ± 0.093	8.028ValVal: 8.028 ± 0.143	1.018ValTrp: 1.018 ± 0.037	1.634ValTyr: 1.634 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	1.424TrpAla: 1.424 ± 0.048	0.148TrpCys: 0.148 ± 0.016	0.728TrpAsp: 0.728 ± 0.029	0.735TrpGlu: 0.735 ± 0.032	0.561TrpPhe: 0.561 ± 0.03	0.987TrpGly: 0.987 ± 0.034	0.367TrpHis: 0.367 ± 0.025	0.819TrpIle: 0.819 ± 0.035	0.556TrpLys: 0.556 ± 0.028	1.558TrpLeu: 1.558 ± 0.049	0.381TrpMet: 0.381 ± 0.024	0.466TrpAsn: 0.466 ± 0.023	0.583TrpPro: 0.583 ± 0.03	0.579TrpGln: 0.579 ± 0.032	0.895TrpArg: 0.895 ± 0.041	0.826TrpSer: 0.826 ± 0.034	0.791TrpThr: 0.791 ± 0.036	1.022TrpVal: 1.022 ± 0.039	0.358TrpTrp: 0.358 ± 0.021	0.307TrpTyr: 0.307 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.311TyrAla: 2.311 ± 0.063	0.197TyrCys: 0.197 ± 0.015	1.318TyrAsp: 1.318 ± 0.041	1.219TyrGlu: 1.219 ± 0.042	0.898TyrPhe: 0.898 ± 0.038	2.049TyrGly: 2.049 ± 0.055	0.502TyrHis: 0.502 ± 0.025	1.104TyrIle: 1.104 ± 0.04	0.727TyrLys: 0.727 ± 0.035	2.246TyrLeu: 2.246 ± 0.062	0.379TyrMet: 0.379 ± 0.022	0.674TyrAsn: 0.674 ± 0.033	1.187TyrPro: 1.187 ± 0.035	0.87TyrGln: 0.87 ± 0.033	1.528TyrArg: 1.528 ± 0.044	1.643TyrSer: 1.643 ± 0.048	1.263TyrThr: 1.263 ± 0.044	1.743TyrVal: 1.743 ± 0.051	0.354TyrTrp: 0.354 ± 0.023	0.589TyrTyr: 0.589 ± 0.038	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2265 proteins (725308 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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