Nocardiopsaceae bacterium YIM 96095

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Nocardiopsaceae; unclassified Nocardiopsaceae

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4542 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N0DQW5|A0A3N0DQW5_9ACTN O-phosphoserine phosphohydrolase OS=Nocardiopsaceae bacterium YIM 96095 OX=2487137 GN=serB PE=3 SV=1
MM1 pKa = 7.24IVSAVVAAVLMAGVPQRR18 pKa = 11.84IDD20 pKa = 3.58DD21 pKa = 5.51LITSGLARR29 pKa = 11.84ALGPSDD35 pKa = 4.41PGTGSNADD43 pKa = 3.55PGEE46 pKa = 4.25QYY48 pKa = 10.44WNQSDD53 pKa = 4.45PDD55 pKa = 4.01SSPATEE61 pKa = 4.68PEE63 pKa = 4.25HH64 pKa = 6.12VTDD67 pKa = 5.11PNHH70 pKa = 7.18PDD72 pKa = 3.52YY73 pKa = 10.95EE74 pKa = 4.66GPPAGHH80 pKa = 7.39PAGPPAPSNAGPDD93 pKa = 3.62PADD96 pKa = 4.01ADD98 pKa = 4.07SEE100 pKa = 4.37AVEE103 pKa = 4.07AAVEE107 pKa = 3.98EE108 pKa = 4.67LEE110 pKa = 5.04GCLSDD115 pKa = 3.85WNEE118 pKa = 3.83DD119 pKa = 3.78APWFTGTCAYY129 pKa = 7.9DD130 pKa = 3.47TYY132 pKa = 11.59KK133 pKa = 10.9DD134 pKa = 3.79LSPEE138 pKa = 3.73EE139 pKa = 3.91LAAVVDD145 pKa = 4.28TMSSQDD151 pKa = 3.47LGRR154 pKa = 11.84LFEE157 pKa = 4.82SGPIPLEE164 pKa = 3.52QEE166 pKa = 4.0YY167 pKa = 10.7FDD169 pKa = 4.58LVEE172 pKa = 4.82EE173 pKa = 4.39IQRR176 pKa = 11.84TAPLDD181 pKa = 3.52TLRR184 pKa = 11.84ALQNSGATPFAQPDD198 pKa = 3.87FEE200 pKa = 5.24GVGGDD205 pKa = 3.58PAGGNSPSNVSYY217 pKa = 11.65GEE219 pKa = 3.67IDD221 pKa = 4.06DD222 pKa = 3.92YY223 pKa = 11.93SLYY226 pKa = 11.26GEE228 pKa = 4.44GDD230 pKa = 3.66EE231 pKa = 4.27PVSWEE236 pKa = 4.1HH237 pKa = 6.44VNQNSLGDD245 pKa = 4.0CWLMATMGAMARR257 pKa = 11.84QNPGHH262 pKa = 5.9VEE264 pKa = 3.8EE265 pKa = 5.29MIQEE269 pKa = 4.33NANGTYY275 pKa = 9.81TVTFPGKK282 pKa = 10.34DD283 pKa = 3.45PVTVTPDD290 pKa = 3.2LPLNPDD296 pKa = 3.5GTPAFAGSNEE306 pKa = 4.36DD307 pKa = 4.8PPVIWPAIVEE317 pKa = 4.03KK318 pKa = 10.52AYY320 pKa = 10.77ADD322 pKa = 4.17LEE324 pKa = 4.53GNDD327 pKa = 3.71YY328 pKa = 11.62SNLEE332 pKa = 3.91NWHH335 pKa = 6.74AGAAMNTFAGGAGMDD350 pKa = 4.4YY351 pKa = 10.7IPDD354 pKa = 3.77VTDD357 pKa = 3.11TTMDD361 pKa = 3.33EE362 pKa = 4.17LAGKK366 pKa = 9.64FEE368 pKa = 4.43NGEE371 pKa = 4.64AITLSTPPTMQLNDD385 pKa = 3.44WPKK388 pKa = 11.0DD389 pKa = 3.57GTGKK393 pKa = 10.94GDD395 pKa = 4.92LVDD398 pKa = 5.67DD399 pKa = 4.68DD400 pKa = 5.67AGTLVSGHH408 pKa = 5.82VYY410 pKa = 10.76FMTGVNRR417 pKa = 11.84DD418 pKa = 3.79DD419 pKa = 3.75GTVTLRR425 pKa = 11.84NPWGSHH431 pKa = 4.63TDD433 pKa = 3.95DD434 pKa = 3.69IVLSLDD440 pKa = 3.48EE441 pKa = 4.63VNDD444 pKa = 3.35NFAAIHH450 pKa = 5.32STNLDD455 pKa = 3.47EE456 pKa = 5.24

Molecular weight: 48.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N0E5S4|A0A3N0E5S4_9ACTN DUF721 domain-containing protein OS=Nocardiopsaceae bacterium YIM 96095 OX=2487137 GN=EFW17_16885 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIIAARR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.68KK38 pKa = 9.79GRR40 pKa = 11.84KK41 pKa = 8.94RR42 pKa = 11.84LTVSHH47 pKa = 6.96

Molecular weight: 5.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4542

0

4542

1459053

29

2590

321.2

34.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.302 ± 0.05

0.762 ± 0.01

6.247 ± 0.035

6.765 ± 0.041

2.752 ± 0.021

9.198 ± 0.034

2.404 ± 0.02

3.366 ± 0.024

1.513 ± 0.023

10.116 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.902 ± 0.015

1.893 ± 0.016

5.939 ± 0.034

2.554 ± 0.02

8.386 ± 0.038

5.421 ± 0.025

6.17 ± 0.024

8.781 ± 0.038

1.509 ± 0.014

2.019 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski