Pontiella sulfatireligans

Taxonomy: cellular organisms; Bacteria; PVC group; Kiritimatiellaeota; Kiritimatiellae; Kiritimatiellales; Pontiellaceae; Pontiella

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5575 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A6C2UL90|A0A6C2UL90_9BACT Uncharacterized protein OS=Pontiella sulfatireligans OX=2750658 GN=SCARR_03069 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 9.86MRR4 pKa = 11.84GITLFAALLGMAIGTQAALPEE25 pKa = 4.61IGDD28 pKa = 4.36TIWLKK33 pKa = 11.17GGTDD37 pKa = 3.15NSYY40 pKa = 11.26VYY42 pKa = 10.89ASGSALVAKK51 pKa = 10.75AEE53 pKa = 4.12TDD55 pKa = 3.45VANALNFVVHH65 pKa = 6.32SADD68 pKa = 3.55GSDD71 pKa = 3.44SNFIFQVADD80 pKa = 3.38TTSYY84 pKa = 11.24VIVVTNNDD92 pKa = 4.37DD93 pKa = 3.56ILTIDD98 pKa = 3.9ATALTNNPLTHH109 pKa = 6.23FTMTEE114 pKa = 3.75ITEE117 pKa = 3.94GTYY120 pKa = 10.73AGTYY124 pKa = 8.86EE125 pKa = 4.53LSCLAHH131 pKa = 7.11ADD133 pKa = 3.81PVVHH137 pKa = 6.48EE138 pKa = 4.99RR139 pKa = 11.84GNTHH143 pKa = 5.5TLKK146 pKa = 10.8AHH148 pKa = 5.12STSGGSIRR156 pKa = 11.84SAWTYY161 pKa = 10.51GVVGAALEE169 pKa = 4.4PPTGLGANTSDD180 pKa = 3.62SQVVLDD186 pKa = 4.11WDD188 pKa = 3.93NDD190 pKa = 3.56VSGLLDD196 pKa = 3.62TYY198 pKa = 9.92TVYY201 pKa = 10.98RR202 pKa = 11.84STNTPVTISDD212 pKa = 3.95TLLGSPSNSQFTDD225 pKa = 3.41TNVVNGTTYY234 pKa = 10.8YY235 pKa = 10.52YY236 pKa = 11.19AVTATDD242 pKa = 4.35GSTEE246 pKa = 3.93TDD248 pKa = 3.11LSNEE252 pKa = 3.96VSATPPTGTAQPKK265 pKa = 9.13RR266 pKa = 11.84LKK268 pKa = 10.89ALGVPAAVLLDD279 pKa = 3.59WADD282 pKa = 3.63DD283 pKa = 4.05TTGHH287 pKa = 6.94LDD289 pKa = 3.25YY290 pKa = 10.29YY291 pKa = 9.17TVYY294 pKa = 10.76RR295 pKa = 11.84STTTPVTTGSPVLTNVTASAFTDD318 pKa = 3.99FDD320 pKa = 4.11VVAGTTNYY328 pKa = 10.46YY329 pKa = 11.0AVTAVGTNGVPPEE342 pKa = 4.22SALSDD347 pKa = 3.67EE348 pKa = 4.67VSAIPFAAVTEE359 pKa = 4.56TVLFQHH365 pKa = 6.95IDD367 pKa = 3.19ATVAASVQTDD377 pKa = 3.47GVTNGMGIVTDD388 pKa = 4.02VVDD391 pKa = 4.11QSTFGNDD398 pKa = 2.95ATDD401 pKa = 3.46VADD404 pKa = 5.2GDD406 pKa = 4.32DD407 pKa = 4.8LGPVLWPSTDD417 pKa = 3.13LFGSGRR423 pKa = 11.84AGFDD427 pKa = 2.72MGTDD431 pKa = 3.17MRR433 pKa = 11.84MLNLFTNGTDD443 pKa = 3.24ALLDD447 pKa = 3.79FTGDD451 pKa = 3.28ASGSDD456 pKa = 3.64GFAMLVAFKK465 pKa = 10.13TRR467 pKa = 11.84TMTEE471 pKa = 3.73GVANIFLNNGPISMEE486 pKa = 3.7YY487 pKa = 10.62DD488 pKa = 3.44GDD490 pKa = 4.16DD491 pKa = 3.67GEE493 pKa = 4.68MTVEE497 pKa = 4.54LGSATLTQSGAGVEE511 pKa = 4.21AGDD514 pKa = 4.0TIVYY518 pKa = 9.66ALNYY522 pKa = 9.29NALTGEE528 pKa = 4.26TTFWDD533 pKa = 3.6SKK535 pKa = 10.71NNEE538 pKa = 4.3EE539 pKa = 4.54VSVTAEE545 pKa = 3.64AHH547 pKa = 6.43GDD549 pKa = 3.65FSGDD553 pKa = 3.47PLQLGGSGRR562 pKa = 11.84DD563 pKa = 3.27SRR565 pKa = 11.84IFDD568 pKa = 3.5GLMGEE573 pKa = 4.38VKK575 pKa = 10.22IFSTKK580 pKa = 10.37LPADD584 pKa = 3.27QFEE587 pKa = 4.43AQRR590 pKa = 11.84DD591 pKa = 3.74ALRR594 pKa = 11.84LKK596 pKa = 9.46WVSADD601 pKa = 3.16ATGFDD606 pKa = 3.72VWVSSWGVNLGSATNDD622 pKa = 3.01WDD624 pKa = 3.87NDD626 pKa = 4.41GINNLAEE633 pKa = 3.98YY634 pKa = 10.53AFAGNPTNGTDD645 pKa = 3.45DD646 pKa = 3.96TYY648 pKa = 11.58TDD650 pKa = 3.97LVTDD654 pKa = 4.26GGLVYY659 pKa = 10.49IHH661 pKa = 6.67PQPKK665 pKa = 9.78NDD667 pKa = 3.6ADD669 pKa = 3.88LNYY672 pKa = 10.99AVLTMDD678 pKa = 4.51NLPYY682 pKa = 9.81DD683 pKa = 3.26TWTDD687 pKa = 3.06EE688 pKa = 4.46GYY690 pKa = 8.14TAVIGTYY697 pKa = 10.04VPAEE701 pKa = 3.55GDD703 pKa = 3.5YY704 pKa = 11.17NYY706 pKa = 10.5VSNSVPTTDD715 pKa = 2.67AVKK718 pKa = 10.31FIRR721 pKa = 11.84ASAEE725 pKa = 3.91YY726 pKa = 10.63

Molecular weight:
76.61 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A6C2US34|A0A6C2US34_9BACT Diaminobutyrate--2-oxoglutarate transaminase OS=Pontiella sulfatireligans OX=2750658 GN=ectB PE=3 SV=1
MM1 pKa = 7.73KK2 pKa = 10.12KK3 pKa = 9.79RR4 pKa = 11.84KK5 pKa = 9.32KK6 pKa = 9.06KK7 pKa = 9.89HH8 pKa = 5.77RR9 pKa = 11.84PRR11 pKa = 11.84KK12 pKa = 9.55HH13 pKa = 5.39NQQHH17 pKa = 5.14TGKK20 pKa = 9.41VAYY23 pKa = 8.78MNSGRR28 pKa = 11.84IKK30 pKa = 10.08STLQTMRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84MSS42 pKa = 2.94

Molecular weight:
5.18 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5575

0

5575

2209931

29

3911

396.4

43.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.858 ± 0.031

1.218 ± 0.012

6.067 ± 0.025

6.303 ± 0.03

4.17 ± 0.015

8.237 ± 0.031

2.174 ± 0.017

5.472 ± 0.022

5.227 ± 0.034

8.987 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.596 ± 0.013

4.2 ± 0.024

4.694 ± 0.022

3.279 ± 0.017

4.949 ± 0.026

6.147 ± 0.027

5.578 ± 0.03

6.801 ± 0.028

1.679 ± 0.015

3.366 ± 0.018

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski