Pyrrhoderma noxium

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Hymenochaetales; Hymenochaetaceae; Pyrrhoderma

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9911 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A286UT56|A0A286UT56_9AGAM Glycosyltransferase family 15 OS=Pyrrhoderma noxium OX=2282107 GN=PNOK_0259200 PE=3 SV=1
MM1 pKa = 7.94IIRR4 pKa = 11.84NGIRR8 pKa = 11.84DD9 pKa = 3.74AFLYY13 pKa = 11.05LLGALFILSRR23 pKa = 11.84VEE25 pKa = 4.15AYY27 pKa = 10.72VPALPSNDD35 pKa = 2.96STLIEE40 pKa = 4.19TSVNQSDD47 pKa = 3.84TSRR50 pKa = 11.84LVLQWFPGSQFSQFVSYY67 pKa = 10.61QLAGADD73 pKa = 3.51STGVNMGALVHH84 pKa = 7.13FSEE87 pKa = 5.26QDD89 pKa = 3.09KK90 pKa = 10.72TNQTTDD96 pKa = 3.68TPWIALVACDD106 pKa = 4.34TNGTAYY112 pKa = 10.9SLEE115 pKa = 4.47DD116 pKa = 4.38DD117 pKa = 4.35VFTLARR123 pKa = 11.84DD124 pKa = 3.74RR125 pKa = 11.84GAVAALLYY133 pKa = 10.59SEE135 pKa = 5.2WSDD138 pKa = 3.13ACIINAEE145 pKa = 4.21YY146 pKa = 10.88ADD148 pKa = 4.69PSTFDD153 pKa = 3.02QVFDD157 pKa = 3.43IFSTKK162 pKa = 9.61TLLSAQTTEE171 pKa = 4.04NAFTNVNQTLYY182 pKa = 10.81SQFDD186 pKa = 3.55ATRR189 pKa = 11.84LNASLTNISNSITTGNVFPGYY210 pKa = 10.62LFATLTTANATVSQSGTDD228 pKa = 3.5SDD230 pKa = 4.48PSSSGSGSSSNGNTDD245 pKa = 2.99LAMIVLYY252 pKa = 10.43AITGAVSVLFCIVILSPTEE271 pKa = 3.54YY272 pKa = 10.67DD273 pKa = 3.25IIVPTSPRR281 pKa = 11.84SKK283 pKa = 10.3VSQDD287 pKa = 3.0PTTSLIQILVSNTSS301 pKa = 2.89

Molecular weight: 32.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A286UWP8|A0A286UWP8_9AGAM Uncharacterized protein OS=Pyrrhoderma noxium OX=2282107 GN=PNOK_0110100 PE=4 SV=1
MM1 pKa = 7.15GATWEE6 pKa = 4.23HH7 pKa = 6.39LKK9 pKa = 10.95DD10 pKa = 3.62DD11 pKa = 4.79AEE13 pKa = 4.22WKK15 pKa = 10.31EE16 pKa = 4.02LDD18 pKa = 3.39RR19 pKa = 11.84QRR21 pKa = 11.84LEE23 pKa = 4.68RR24 pKa = 11.84GEE26 pKa = 4.14QPASRR31 pKa = 11.84ISSIRR36 pKa = 11.84SSSSPDD42 pKa = 2.9QRR44 pKa = 11.84QPSTPANQRR53 pKa = 11.84PSHH56 pKa = 6.68ASPSIAQRR64 pKa = 11.84PPPISAPQRR73 pKa = 11.84QVPPPATQRR82 pKa = 11.84QPAYY86 pKa = 8.33PAHH89 pKa = 7.57PIQQPRR95 pKa = 11.84STTSASVGIATNSSARR111 pKa = 11.84LASTATYY118 pKa = 10.69SSGNRR123 pKa = 11.84APEE126 pKa = 3.94AAEE129 pKa = 3.99ATSSSSASDD138 pKa = 3.41KK139 pKa = 10.75TCEE142 pKa = 3.99LCHH145 pKa = 6.68KK146 pKa = 10.12RR147 pKa = 11.84FTRR150 pKa = 11.84TSDD153 pKa = 3.2LTRR156 pKa = 11.84HH157 pKa = 5.69IDD159 pKa = 3.15HH160 pKa = 7.31RR161 pKa = 11.84KK162 pKa = 6.73TLTVVTQCQRR172 pKa = 11.84YY173 pKa = 8.62RR174 pKa = 11.84RR175 pKa = 3.87

Molecular weight: 19.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9911

0

9911

4934286

52

4846

497.9

55.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.165 ± 0.022

1.155 ± 0.009

5.535 ± 0.016

6.271 ± 0.027

3.758 ± 0.015

6.514 ± 0.031

2.311 ± 0.011

5.449 ± 0.023

5.086 ± 0.023

9.007 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.921 ± 0.009

4.201 ± 0.012

5.926 ± 0.03

3.642 ± 0.021

5.997 ± 0.021

9.803 ± 0.032

6.167 ± 0.02

6.006 ± 0.017

1.281 ± 0.009

2.805 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski