Sewage-associated circular DNA virus-5

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075IZU0|A0A075IZU0_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-5 OX=1519394 PE=4 SV=1
MM1 pKa = 8.01EE2 pKa = 5.24DD3 pKa = 3.22TLRR6 pKa = 11.84TPDD9 pKa = 3.67SEE11 pKa = 4.65TVKK14 pKa = 11.06AKK16 pKa = 10.4GDD18 pKa = 3.29QRR20 pKa = 11.84YY21 pKa = 9.77RR22 pKa = 11.84NVFYY26 pKa = 10.13TYY28 pKa = 11.26NNPTKK33 pKa = 10.29TDD35 pKa = 4.15EE36 pKa = 3.84EE37 pKa = 4.23WMDD40 pKa = 3.62YY41 pKa = 10.23VRR43 pKa = 11.84EE44 pKa = 3.96RR45 pKa = 11.84VPIAFHH51 pKa = 5.7VCALEE56 pKa = 4.4EE57 pKa = 4.51GDD59 pKa = 5.36LNHH62 pKa = 6.73TPHH65 pKa = 6.43LQGYY69 pKa = 7.46MEE71 pKa = 4.86FRR73 pKa = 11.84QQVRR77 pKa = 11.84KK78 pKa = 9.6SQLITAFGTHH88 pKa = 5.18FWFRR92 pKa = 11.84PRR94 pKa = 11.84RR95 pKa = 11.84GTALEE100 pKa = 4.1AYY102 pKa = 7.63EE103 pKa = 4.31YY104 pKa = 8.39CTKK107 pKa = 10.32SGKK110 pKa = 9.1YY111 pKa = 9.95AEE113 pKa = 4.91RR114 pKa = 11.84SHH116 pKa = 5.42EE117 pKa = 4.32TKK119 pKa = 10.59AGPWEE124 pKa = 4.0EE125 pKa = 4.52GKK127 pKa = 10.57RR128 pKa = 11.84SSPGHH133 pKa = 5.34RR134 pKa = 11.84TDD136 pKa = 3.81IEE138 pKa = 4.36AAVHH142 pKa = 5.74TLLKK146 pKa = 10.77DD147 pKa = 3.73GLQSAVTQHH156 pKa = 5.45TQTMVQWGKK165 pKa = 10.85GIKK168 pKa = 9.64QVVKK172 pKa = 10.73DD173 pKa = 4.03MRR175 pKa = 11.84DD176 pKa = 3.38PPPIEE181 pKa = 3.8RR182 pKa = 11.84TIEE185 pKa = 4.05VILLHH190 pKa = 6.67GPGGVGKK197 pKa = 9.74DD198 pKa = 3.13HH199 pKa = 5.81QVYY202 pKa = 8.01VTGEE206 pKa = 3.43KK207 pKa = 9.88SQRR210 pKa = 11.84TFHH213 pKa = 6.97LLPGDD218 pKa = 3.85LEE220 pKa = 4.11NRR222 pKa = 11.84TPYY225 pKa = 10.22EE226 pKa = 4.18GQSVLILDD234 pKa = 4.34EE235 pKa = 4.46FDD237 pKa = 4.06GASSRR242 pKa = 11.84ADD244 pKa = 3.07VRR246 pKa = 11.84TLNRR250 pKa = 11.84ILDD253 pKa = 3.53KK254 pKa = 10.61WPYY257 pKa = 9.64DD258 pKa = 3.84LKK260 pKa = 11.22GLYY263 pKa = 9.43TNEE266 pKa = 3.71PARR269 pKa = 11.84WTRR272 pKa = 11.84VYY274 pKa = 10.66ILTNSHH280 pKa = 5.87PLKK283 pKa = 10.25WYY285 pKa = 9.91DD286 pKa = 3.01WDD288 pKa = 4.19KK289 pKa = 11.52EE290 pKa = 4.42SLLSSHH296 pKa = 6.57TPNQQGVALEE306 pKa = 3.94PRR308 pKa = 11.84ARR310 pKa = 11.84SARR313 pKa = 11.84TRR315 pKa = 3.29

Molecular weight: 36.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075IZU0|A0A075IZU0_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-5 OX=1519394 PE=4 SV=1
MM1 pKa = 7.51ARR3 pKa = 11.84YY4 pKa = 6.71TKK6 pKa = 10.37KK7 pKa = 9.98SSSYY11 pKa = 9.2RR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 9.96GVRR17 pKa = 11.84KK18 pKa = 7.26TRR20 pKa = 11.84AARR23 pKa = 11.84RR24 pKa = 11.84TYY26 pKa = 9.91RR27 pKa = 11.84KK28 pKa = 9.43KK29 pKa = 10.35GKK31 pKa = 9.53RR32 pKa = 11.84PSRR35 pKa = 11.84SRR37 pKa = 11.84IQTKK41 pKa = 10.34RR42 pKa = 11.84LGSRR46 pKa = 11.84YY47 pKa = 9.32PLGQKK52 pKa = 10.52AIIKK56 pKa = 10.07LNYY59 pKa = 8.68IGNGQVTGGGVLGYY73 pKa = 10.69GSGGTYY79 pKa = 9.26TLNSLSSPCKK89 pKa = 10.53NGTTDD94 pKa = 2.8NRR96 pKa = 11.84AAKK99 pKa = 9.62FLPNSFEE106 pKa = 4.36KK107 pKa = 11.06YY108 pKa = 9.13RR109 pKa = 11.84VTGMSYY115 pKa = 10.47RR116 pKa = 11.84IAATLVNTATPVPGYY131 pKa = 8.53MWFVPFEE138 pKa = 4.67AGTTTMPGTSAYY150 pKa = 10.24GDD152 pKa = 3.73QGEE155 pKa = 4.3MAQIPGAKK163 pKa = 9.5FKK165 pKa = 11.12NLEE168 pKa = 3.71AWNASGKK175 pKa = 8.41MAYY178 pKa = 10.15LKK180 pKa = 10.68GYY182 pKa = 11.21VNMKK186 pKa = 9.01QMRR189 pKa = 11.84GDD191 pKa = 3.44GRR193 pKa = 11.84SAGDD197 pKa = 3.77DD198 pKa = 3.75DD199 pKa = 4.09FTGTVGISGNWTDD212 pKa = 5.37PSRR215 pKa = 11.84LWDD218 pKa = 3.63YY219 pKa = 11.78AFGWSSINRR228 pKa = 11.84TPVPTTQAMHH238 pKa = 5.54YY239 pKa = 9.56SIRR242 pKa = 11.84TTYY245 pKa = 9.86YY246 pKa = 9.94VEE248 pKa = 4.3FFSPSMNGIEE258 pKa = 3.96

Molecular weight: 28.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

573

258

315

286.5

32.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.283 ± 0.633

0.524 ± 0.08

4.712 ± 0.944

5.236 ± 1.704

2.967 ± 0.305

9.25 ± 1.62

2.443 ± 1.204

3.49 ± 0.226

6.283 ± 0.406

6.283 ± 1.183

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.618 ± 0.737

3.839 ± 0.702

5.41 ± 0.218

3.839 ± 0.66

8.202 ± 0.037

6.632 ± 1.11

9.075 ± 0.36

5.236 ± 0.569

2.269 ± 0.194

5.41 ± 0.691
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski