Phellinidium pouzarii

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Hymenochaetales; Hymenochaetaceae; Phellinidium

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8697 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S4L1Y3|A0A4S4L1Y3_9AGAM Uncharacterized protein OS=Phellinidium pouzarii OX=167371 GN=EW145_g4877 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.48LSITFCVSTLVASTAAAPLDD22 pKa = 3.94LFWRR26 pKa = 11.84QVVADD31 pKa = 4.88GLTLTQIDD39 pKa = 4.27ALTPQFGHH47 pKa = 6.83AADD50 pKa = 4.04VNPTGTGNCDD60 pKa = 2.86GAVNDD65 pKa = 4.45TNGLPIEE72 pKa = 4.8VPCSCPPPRR81 pKa = 11.84ATFIQSLAANIAAGHH96 pKa = 6.03AVNNPSVAVTFPTDD110 pKa = 2.93NSTASILARR119 pKa = 11.84FNAASITLQNEE130 pKa = 3.59NGEE133 pKa = 4.39GVGCPEE139 pKa = 5.72SSTTWTAQAAAIQAGAKK156 pKa = 9.98DD157 pKa = 4.06NNAPTAAAPPSSSAPVSASSPATTVAAPASSAVTTASASAPSTTGADD204 pKa = 3.79GLSLAQIDD212 pKa = 4.2ALTPQFGHH220 pKa = 7.14DD221 pKa = 3.51ADD223 pKa = 4.28VNPTGTGNCDD233 pKa = 3.29GAVDD237 pKa = 4.58DD238 pKa = 5.72ANGDD242 pKa = 4.37PILVPCDD249 pKa = 3.58CPPPRR254 pKa = 11.84NQFIQDD260 pKa = 3.83LTANIAAGFAVNNPSVAITFPTDD283 pKa = 2.67NSTNSILARR292 pKa = 11.84FNAASVTLQNTNGPGVGCPISSTTWTAQANAIRR325 pKa = 11.84DD326 pKa = 4.02GQQSRR331 pKa = 11.84VAIATTLSVSTEE343 pKa = 4.21SSVSTSPAASSVVAGCPAPEE363 pKa = 4.07PTPTSSEE370 pKa = 4.16VPTPSSIVSASPVSSTDD387 pKa = 3.01VTSADD392 pKa = 3.92GLSLAQIDD400 pKa = 4.3ALTPRR405 pKa = 11.84LGFSSGVNPTGTGNCDD421 pKa = 3.09GAVNGANGQPIEE433 pKa = 4.7VPCSCPPARR442 pKa = 11.84DD443 pKa = 3.56VFIQSLAQDD452 pKa = 3.83IAAGHH457 pKa = 6.21AVNNPSVSVSFPTDD471 pKa = 2.78NSTSSQITRR480 pKa = 11.84IQSSLVALQNLNGPGQGCPAASTTLSAQLAALQGG514 pKa = 3.57

Molecular weight: 50.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S4KXC8|A0A4S4KXC8_9AGAM Uncharacterized protein OS=Phellinidium pouzarii OX=167371 GN=EW145_g6376 PE=4 SV=1
MM1 pKa = 7.44SAPAAANTRR10 pKa = 11.84RR11 pKa = 11.84WAPTSDD17 pKa = 3.6RR18 pKa = 11.84PNSIDD23 pKa = 3.47PAPVHH28 pKa = 6.14SRR30 pKa = 11.84PAFSSFSSFSSSISSSLSSAFGVGSSRR57 pKa = 11.84HH58 pKa = 5.27SRR60 pKa = 11.84SNSNATQSSQLNPNSHH76 pKa = 7.23PLRR79 pKa = 11.84PASAAPTAHH88 pKa = 5.99GHH90 pKa = 6.35AQAKK94 pKa = 7.28WLPRR98 pKa = 11.84TLTPGPGXRR107 pKa = 11.84RR108 pKa = 11.84LLITHH113 pKa = 7.12APAIVRR119 pKa = 11.84RR120 pKa = 11.84RR121 pKa = 11.84ARR123 pKa = 11.84LPPPQPQQLTRR134 pKa = 11.84LQTTXPLPPLPPLPXSRR151 pKa = 11.84QHH153 pKa = 6.52SSVLVGYY160 pKa = 7.03TKK162 pKa = 10.48PRR164 pKa = 11.84AKK166 pKa = 10.51GG167 pKa = 3.21

Molecular weight: 17.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8697

0

8697

4555891

33

4853

523.8

57.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.591 ± 0.023

1.174 ± 0.008

5.744 ± 0.016

6.032 ± 0.025

3.823 ± 0.014

6.226 ± 0.023

2.524 ± 0.011

4.927 ± 0.019

4.666 ± 0.022

9.183 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.064 ± 0.007

3.676 ± 0.013

6.198 ± 0.028

3.647 ± 0.018

6.197 ± 0.02

9.157 ± 0.038

6.05 ± 0.017

6.2 ± 0.019

1.276 ± 0.009

2.599 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski