Amino acid dipepetide frequency for Caenimonas sp. HX-9-20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.503AlaAla: 19.503 ± 0.148
1.395AlaCys: 1.395 ± 0.031
6.426AlaAsp: 6.426 ± 0.068
6.848AlaGlu: 6.848 ± 0.065
4.225AlaPhe: 4.225 ± 0.054
12.12AlaGly: 12.12 ± 0.121
2.699AlaHis: 2.699 ± 0.041
5.155AlaIle: 5.155 ± 0.052
4.133AlaLys: 4.133 ± 0.065
14.644AlaLeu: 14.644 ± 0.124
3.681AlaMet: 3.681 ± 0.05
2.861AlaAsn: 2.861 ± 0.05
6.746AlaPro: 6.746 ± 0.09
5.714AlaGln: 5.714 ± 0.066
9.782AlaArg: 9.782 ± 0.079
6.824AlaSer: 6.824 ± 0.068
6.284AlaThr: 6.284 ± 0.067
9.44AlaVal: 9.44 ± 0.071
2.109AlaTrp: 2.109 ± 0.043
2.415AlaTyr: 2.415 ± 0.039
0.0AlaXaa: 0.0 ± 0.0
Cys
1.238CysAla: 1.238 ± 0.027
0.139CysCys: 0.139 ± 0.009
0.544CysAsp: 0.544 ± 0.02
0.523CysGlu: 0.523 ± 0.018
0.301CysPhe: 0.301 ± 0.012
1.04CysGly: 1.04 ± 0.026
0.266CysHis: 0.266 ± 0.013
0.41CysIle: 0.41 ± 0.015
0.248CysLys: 0.248 ± 0.012
0.798CysLeu: 0.798 ± 0.022
0.216CysMet: 0.216 ± 0.009
0.226CysAsn: 0.226 ± 0.01
0.477CysPro: 0.477 ± 0.018
0.245CysGln: 0.245 ± 0.012
0.625CysArg: 0.625 ± 0.02
0.524CysSer: 0.524 ± 0.017
0.471CysThr: 0.471 ± 0.015
0.734CysVal: 0.734 ± 0.022
0.138CysTrp: 0.138 ± 0.009
0.203CysTyr: 0.203 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
6.988AspAla: 6.988 ± 0.07
0.461AspCys: 0.461 ± 0.017
2.611AspAsp: 2.611 ± 0.048
3.083AspGlu: 3.083 ± 0.05
2.02AspPhe: 2.02 ± 0.038
4.607AspGly: 4.607 ± 0.062
1.132AspHis: 1.132 ± 0.029
2.203AspIle: 2.203 ± 0.039
1.679AspLys: 1.679 ± 0.034
5.214AspLeu: 5.214 ± 0.059
1.142AspMet: 1.142 ± 0.025
1.11AspAsn: 1.11 ± 0.03
3.004AspPro: 3.004 ± 0.044
1.538AspGln: 1.538 ± 0.032
3.42AspArg: 3.42 ± 0.051
2.104AspSer: 2.104 ± 0.037
2.344AspThr: 2.344 ± 0.04
3.981AspVal: 3.981 ± 0.051
0.94AspTrp: 0.94 ± 0.022
1.297AspTyr: 1.297 ± 0.029
0.0AspXaa: 0.0 ± 0.0
Glu
7.175GluAla: 7.175 ± 0.08
0.435GluCys: 0.435 ± 0.017
2.285GluAsp: 2.285 ± 0.037
2.766GluGlu: 2.766 ± 0.049
1.926GluPhe: 1.926 ± 0.034
3.979GluGly: 3.979 ± 0.051
1.314GluHis: 1.314 ± 0.028
2.564GluIle: 2.564 ± 0.039
1.926GluLys: 1.926 ± 0.037
5.957GluLeu: 5.957 ± 0.055
1.312GluMet: 1.312 ± 0.029
1.181GluAsn: 1.181 ± 0.025
2.67GluPro: 2.67 ± 0.042
2.472GluGln: 2.472 ± 0.038
4.742GluArg: 4.742 ± 0.057
2.513GluSer: 2.513 ± 0.039
2.515GluThr: 2.515 ± 0.041
4.293GluVal: 4.293 ± 0.055
0.791GluTrp: 0.791 ± 0.02
1.01GluTyr: 1.01 ± 0.026
0.0GluXaa: 0.0 ± 0.0
Phe
4.422PheAla: 4.422 ± 0.059
0.369PheCys: 0.369 ± 0.015
2.4PheAsp: 2.4 ± 0.035
2.08PheGlu: 2.08 ± 0.031
1.275PhePhe: 1.275 ± 0.031
3.512PheGly: 3.512 ± 0.047
0.798PheHis: 0.798 ± 0.021
1.41PheIle: 1.41 ± 0.028
1.108PheLys: 1.108 ± 0.026
3.129PheLeu: 3.129 ± 0.044
0.81PheMet: 0.81 ± 0.02
1.049PheAsn: 1.049 ± 0.025
1.604PhePro: 1.604 ± 0.031
1.127PheGln: 1.127 ± 0.023
2.073PheArg: 2.073 ± 0.036
2.003PheSer: 2.003 ± 0.033
1.955PheThr: 1.955 ± 0.033
2.713PheVal: 2.713 ± 0.042
0.524PheTrp: 0.524 ± 0.019
0.836PheTyr: 0.836 ± 0.022
0.0PheXaa: 0.0 ± 0.0
Gly
10.008GlyAla: 10.008 ± 0.091
0.892GlyCys: 0.892 ± 0.025
4.088GlyAsp: 4.088 ± 0.067
4.663GlyGlu: 4.663 ± 0.048
3.369GlyPhe: 3.369 ± 0.046
7.767GlyGly: 7.767 ± 0.125
1.979GlyHis: 1.979 ± 0.035
4.056GlyIle: 4.056 ± 0.054
3.284GlyLys: 3.284 ± 0.046
8.823GlyLeu: 8.823 ± 0.081
2.421GlyMet: 2.421 ± 0.044
2.246GlyAsn: 2.246 ± 0.055
3.526GlyPro: 3.526 ± 0.046
3.341GlyGln: 3.341 ± 0.045
5.928GlyArg: 5.928 ± 0.061
4.8GlySer: 4.8 ± 0.056
4.813GlyThr: 4.813 ± 0.078
6.66GlyVal: 6.66 ± 0.067
1.58GlyTrp: 1.58 ± 0.032
2.297GlyTyr: 2.297 ± 0.041
0.0GlyXaa: 0.0 ± 0.0
His
2.939HisAla: 2.939 ± 0.047
0.306HisCys: 0.306 ± 0.013
1.159HisAsp: 1.159 ± 0.029
1.193HisGlu: 1.193 ± 0.028
0.89HisPhe: 0.89 ± 0.022
2.228HisGly: 2.228 ± 0.034
0.598HisHis: 0.598 ± 0.021
0.826HisIle: 0.826 ± 0.025
0.566HisLys: 0.566 ± 0.017
2.268HisLeu: 2.268 ± 0.042
0.509HisMet: 0.509 ± 0.017
0.468HisAsn: 0.468 ± 0.019
1.502HisPro: 1.502 ± 0.03
0.716HisGln: 0.716 ± 0.018
1.599HisArg: 1.599 ± 0.031
1.005HisSer: 1.005 ± 0.023
1.004HisThr: 1.004 ± 0.024
1.722HisVal: 1.722 ± 0.033
0.445HisTrp: 0.445 ± 0.016
0.581HisTyr: 0.581 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
5.974IleAla: 5.974 ± 0.054
0.427IleCys: 0.427 ± 0.018
2.732IleAsp: 2.732 ± 0.043
2.807IleGlu: 2.807 ± 0.045
1.271IlePhe: 1.271 ± 0.023
3.888IleGly: 3.888 ± 0.044
0.884IleHis: 0.884 ± 0.023
1.337IleIle: 1.337 ± 0.032
1.26IleLys: 1.26 ± 0.028
3.191IleLeu: 3.191 ± 0.046
0.693IleMet: 0.693 ± 0.019
1.154IleAsn: 1.154 ± 0.029
2.019IlePro: 2.019 ± 0.035
1.302IleGln: 1.302 ± 0.026
2.622IleArg: 2.622 ± 0.045
2.124IleSer: 2.124 ± 0.032
2.33IleThr: 2.33 ± 0.047
3.422IleVal: 3.422 ± 0.053
0.446IleTrp: 0.446 ± 0.016
0.912IleTyr: 0.912 ± 0.023
0.0IleXaa: 0.0 ± 0.0
Lys
4.145LysAla: 4.145 ± 0.064
0.173LysCys: 0.173 ± 0.009
1.689LysAsp: 1.689 ± 0.035
1.618LysGlu: 1.618 ± 0.034
1.027LysPhe: 1.027 ± 0.025
2.425LysGly: 2.425 ± 0.045
0.708LysHis: 0.708 ± 0.02
1.357LysIle: 1.357 ± 0.03
1.387LysLys: 1.387 ± 0.037
3.667LysLeu: 3.667 ± 0.051
0.801LysMet: 0.801 ± 0.023
0.852LysAsn: 0.852 ± 0.024
2.144LysPro: 2.144 ± 0.037
1.205LysGln: 1.205 ± 0.026
2.277LysArg: 2.277 ± 0.036
1.741LysSer: 1.741 ± 0.032
1.804LysThr: 1.804 ± 0.034
2.621LysVal: 2.621 ± 0.048
0.359LysTrp: 0.359 ± 0.015
0.669LysTyr: 0.669 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
15.806LeuAla: 15.806 ± 0.118
0.974LeuCys: 0.974 ± 0.022
5.486LeuAsp: 5.486 ± 0.064
5.358LeuGlu: 5.358 ± 0.059
3.348LeuPhe: 3.348 ± 0.047
8.697LeuGly: 8.697 ± 0.08
2.386LeuHis: 2.386 ± 0.042
3.748LeuIle: 3.748 ± 0.05
3.527LeuLys: 3.527 ± 0.05
11.158LeuLeu: 11.158 ± 0.116
2.357LeuMet: 2.357 ± 0.035
2.465LeuAsn: 2.465 ± 0.04
6.127LeuPro: 6.127 ± 0.062
4.162LeuGln: 4.162 ± 0.05
8.041LeuArg: 8.041 ± 0.075
5.287LeuSer: 5.287 ± 0.059
4.999LeuThr: 4.999 ± 0.061
8.196LeuVal: 8.196 ± 0.082
1.392LeuTrp: 1.392 ± 0.031
1.899LeuTyr: 1.899 ± 0.034
0.0LeuXaa: 0.0 ± 0.0
Met
3.154MetAla: 3.154 ± 0.04
0.185MetCys: 0.185 ± 0.01
1.156MetAsp: 1.156 ± 0.027
1.13MetGlu: 1.13 ± 0.026
0.724MetPhe: 0.724 ± 0.02
1.918MetGly: 1.918 ± 0.038
0.555MetHis: 0.555 ± 0.017
0.901MetIle: 0.901 ± 0.023
1.126MetLys: 1.126 ± 0.025
2.682MetLeu: 2.682 ± 0.042
0.56MetMet: 0.56 ± 0.02
0.851MetAsn: 0.851 ± 0.02
1.497MetPro: 1.497 ± 0.032
1.127MetGln: 1.127 ± 0.022
1.843MetArg: 1.843 ± 0.036
1.386MetSer: 1.386 ± 0.027
1.549MetThr: 1.549 ± 0.03
1.775MetVal: 1.775 ± 0.032
0.211MetTrp: 0.211 ± 0.01
0.381MetTyr: 0.381 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
3.112AsnAla: 3.112 ± 0.044
0.25AsnCys: 0.25 ± 0.012
1.296AsnAsp: 1.296 ± 0.036
1.173AsnGlu: 1.173 ± 0.029
0.913AsnPhe: 0.913 ± 0.025
2.12AsnGly: 2.12 ± 0.045
0.489AsnHis: 0.489 ± 0.017
1.115AsnIle: 1.115 ± 0.029
0.774AsnLys: 0.774 ± 0.021
2.513AsnLeu: 2.513 ± 0.041
0.547AsnMet: 0.547 ± 0.018
0.684AsnAsn: 0.684 ± 0.022
1.703AsnPro: 1.703 ± 0.028
0.824AsnGln: 0.824 ± 0.023
1.608AsnArg: 1.608 ± 0.034
1.1AsnSer: 1.1 ± 0.029
1.274AsnThr: 1.274 ± 0.037
1.921AsnVal: 1.921 ± 0.039
0.399AsnTrp: 0.399 ± 0.017
0.623AsnTyr: 0.623 ± 0.017
0.0AsnXaa: 0.0 ± 0.0
Pro
7.983ProAla: 7.983 ± 0.087
0.376ProCys: 0.376 ± 0.015
3.095ProAsp: 3.095 ± 0.039
3.392ProGlu: 3.392 ± 0.051
1.801ProPhe: 1.801 ± 0.036
5.243ProGly: 5.243 ± 0.066
1.163ProHis: 1.163 ± 0.029
1.86ProIle: 1.86 ± 0.035
1.633ProLys: 1.633 ± 0.032
5.208ProLeu: 5.208 ± 0.062
1.319ProMet: 1.319 ± 0.029
1.245ProAsn: 1.245 ± 0.033
3.145ProPro: 3.145 ± 0.053
2.159ProGln: 2.159 ± 0.035
3.359ProArg: 3.359 ± 0.043
2.676ProSer: 2.676 ± 0.04
2.581ProThr: 2.581 ± 0.045
4.44ProVal: 4.44 ± 0.055
0.845ProTrp: 0.845 ± 0.024
1.147ProTyr: 1.147 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
5.705GlnAla: 5.705 ± 0.061
0.306GlnCys: 0.306 ± 0.014
1.578GlnAsp: 1.578 ± 0.028
1.698GlnGlu: 1.698 ± 0.034
1.246GlnPhe: 1.246 ± 0.028
3.185GlnGly: 3.185 ± 0.047
0.886GlnHis: 0.886 ± 0.021
1.446GlnIle: 1.446 ± 0.024
1.172GlnLys: 1.172 ± 0.03
4.224GlnLeu: 4.224 ± 0.057
0.942GlnMet: 0.942 ± 0.022
0.753GlnAsn: 0.753 ± 0.023
2.331GlnPro: 2.331 ± 0.037
1.802GlnGln: 1.802 ± 0.035
3.333GlnArg: 3.333 ± 0.048
1.833GlnSer: 1.833 ± 0.031
1.596GlnThr: 1.596 ± 0.033
3.185GlnVal: 3.185 ± 0.046
0.645GlnTrp: 0.645 ± 0.02
0.712GlnTyr: 0.712 ± 0.022
0.0GlnXaa: 0.0 ± 0.0
Arg
8.427ArgAla: 8.427 ± 0.073
0.643ArgCys: 0.643 ± 0.017
3.695ArgAsp: 3.695 ± 0.042
4.547ArgGlu: 4.547 ± 0.063
2.877ArgPhe: 2.877 ± 0.042
5.166ArgGly: 5.166 ± 0.052
1.918ArgHis: 1.918 ± 0.036
3.538ArgIle: 3.538 ± 0.044
2.425ArgLys: 2.425 ± 0.037
7.841ArgLeu: 7.841 ± 0.076
2.052ArgMet: 2.052 ± 0.034
1.828ArgAsn: 1.828 ± 0.034
3.54ArgPro: 3.54 ± 0.049
2.923ArgGln: 2.923 ± 0.039
5.441ArgArg: 5.441 ± 0.069
3.595ArgSer: 3.595 ± 0.046
3.368ArgThr: 3.368 ± 0.043
5.277ArgVal: 5.277 ± 0.057
1.285ArgTrp: 1.285 ± 0.029
1.7ArgTyr: 1.7 ± 0.037
0.0ArgXaa: 0.0 ± 0.0
Ser
6.242SerAla: 6.242 ± 0.061
0.468SerCys: 0.468 ± 0.019
2.392SerAsp: 2.392 ± 0.042
2.399SerGlu: 2.399 ± 0.036
1.988SerPhe: 1.988 ± 0.031
5.133SerGly: 5.133 ± 0.057
1.137SerHis: 1.137 ± 0.029
2.188SerIle: 2.188 ± 0.04
1.435SerLys: 1.435 ± 0.03
5.271SerLeu: 5.271 ± 0.063
1.388SerMet: 1.388 ± 0.031
1.24SerAsn: 1.24 ± 0.029
2.847SerPro: 2.847 ± 0.041
1.743SerGln: 1.743 ± 0.03
3.506SerArg: 3.506 ± 0.039
2.776SerSer: 2.776 ± 0.047
2.708SerThr: 2.708 ± 0.041
3.784SerVal: 3.784 ± 0.05
0.774SerTrp: 0.774 ± 0.021
1.23SerTyr: 1.23 ± 0.028
0.0SerXaa: 0.0 ± 0.0
Thr
6.143ThrAla: 6.143 ± 0.072
0.437ThrCys: 0.437 ± 0.016
2.374ThrAsp: 2.374 ± 0.04
2.378ThrGlu: 2.378 ± 0.035
1.792ThrPhe: 1.792 ± 0.035
4.875ThrGly: 4.875 ± 0.062
1.15ThrHis: 1.15 ± 0.024
2.049ThrIle: 2.049 ± 0.037
1.267ThrLys: 1.267 ± 0.027
5.895ThrLeu: 5.895 ± 0.073
1.211ThrMet: 1.211 ± 0.028
1.133ThrAsn: 1.133 ± 0.028
3.474ThrPro: 3.474 ± 0.044
1.774ThrGln: 1.774 ± 0.033
3.274ThrArg: 3.274 ± 0.038
2.571ThrSer: 2.571 ± 0.042
2.682ThrThr: 2.682 ± 0.053
4.276ThrVal: 4.276 ± 0.062
0.725ThrTrp: 0.725 ± 0.023
1.05ThrTyr: 1.05 ± 0.025
0.0ThrXaa: 0.0 ± 0.0
Val
10.018ValAla: 10.018 ± 0.083
0.71ValCys: 0.71 ± 0.022
3.979ValAsp: 3.979 ± 0.046
4.393ValGlu: 4.393 ± 0.056
2.724ValPhe: 2.724 ± 0.044
5.607ValGly: 5.607 ± 0.061
1.669ValHis: 1.669 ± 0.035
3.256ValIle: 3.256 ± 0.046
2.558ValLys: 2.558 ± 0.047
8.528ValLeu: 8.528 ± 0.086
1.892ValMet: 1.892 ± 0.03
2.063ValAsn: 2.063 ± 0.035
4.426ValPro: 4.426 ± 0.045
2.925ValGln: 2.925 ± 0.042
5.667ValArg: 5.667 ± 0.052
3.893ValSer: 3.893 ± 0.046
4.296ValThr: 4.296 ± 0.054
6.456ValVal: 6.456 ± 0.076
0.957ValTrp: 0.957 ± 0.022
1.534ValTyr: 1.534 ± 0.03
0.0ValXaa: 0.0 ± 0.0
Trp
1.492TrpAla: 1.492 ± 0.029
0.189TrpCys: 0.189 ± 0.012
0.683TrpAsp: 0.683 ± 0.02
0.607TrpGlu: 0.607 ± 0.018
0.586TrpPhe: 0.586 ± 0.018
1.038TrpGly: 1.038 ± 0.026
0.366TrpHis: 0.366 ± 0.016
0.667TrpIle: 0.667 ± 0.022
0.525TrpLys: 0.525 ± 0.017
2.102TrpLeu: 2.102 ± 0.039
0.432TrpMet: 0.432 ± 0.017
0.462TrpAsn: 0.462 ± 0.016
0.81TrpPro: 0.81 ± 0.022
0.711TrpGln: 0.711 ± 0.02
1.3TrpArg: 1.3 ± 0.03
0.777TrpSer: 0.777 ± 0.022
0.799TrpThr: 0.799 ± 0.022
1.078TrpVal: 1.078 ± 0.025
0.304TrpTrp: 0.304 ± 0.014
0.313TrpTyr: 0.313 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.528TyrAla: 2.528 ± 0.038
0.233TyrCys: 0.233 ± 0.012
1.211TyrAsp: 1.211 ± 0.027
1.172TyrGlu: 1.172 ± 0.028
0.893TyrPhe: 0.893 ± 0.023
1.994TyrGly: 1.994 ± 0.038
0.427TyrHis: 0.427 ± 0.015
0.712TyrIle: 0.712 ± 0.02
0.633TyrLys: 0.633 ± 0.02
2.301TyrLeu: 2.301 ± 0.042
0.427TyrMet: 0.427 ± 0.014
0.57TyrAsn: 0.57 ± 0.017
1.076TyrPro: 1.076 ± 0.025
0.766TyrGln: 0.766 ± 0.019
1.646TyrArg: 1.646 ± 0.035
1.114TyrSer: 1.114 ± 0.03
1.158TyrThr: 1.158 ± 0.024
1.577TyrVal: 1.577 ± 0.029
0.373TyrTrp: 0.373 ± 0.014
0.519TyrTyr: 0.519 ± 0.018
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5612 proteins (1817594 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski