Tortoise microvirus 14

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W653|A0A4P8W653_9VIRU Uncharacterized protein OS=Tortoise microvirus 14 OX=2583115 PE=4 SV=1
MM1 pKa = 7.54SKK3 pKa = 9.0TVNIGGDD10 pKa = 3.26RR11 pKa = 11.84LGSGNKK17 pKa = 9.15MNVQLHH23 pKa = 6.16GYY25 pKa = 9.01SRR27 pKa = 11.84SNHH30 pKa = 6.01DD31 pKa = 3.97LSYY34 pKa = 10.17VWRR37 pKa = 11.84STMSPGTLVPFMVQVALPGDD57 pKa = 3.65SHH59 pKa = 8.64DD60 pKa = 3.91IEE62 pKa = 5.41LNAEE66 pKa = 4.33VMTHH70 pKa = 5.88PTLGPLFGSYY80 pKa = 10.52KK81 pKa = 10.09LQLDD85 pKa = 3.8VFSIPFRR92 pKa = 11.84LYY94 pKa = 11.06NSWLHH99 pKa = 5.48NNRR102 pKa = 11.84LGIGLDD108 pKa = 3.27MSKK111 pKa = 10.93VKK113 pKa = 10.65LPKK116 pKa = 10.47LKK118 pKa = 10.81FNLPYY123 pKa = 10.53INATGSNIPVDD134 pKa = 3.91EE135 pKa = 4.04QQINPSSLLAYY146 pKa = 10.32LGIRR150 pKa = 11.84GGIRR154 pKa = 11.84TNTGQISSPYY164 pKa = 9.67RR165 pKa = 11.84RR166 pKa = 11.84NTKK169 pKa = 8.89MAIPYY174 pKa = 8.91LAYY177 pKa = 8.98WDD179 pKa = 3.77IYY181 pKa = 10.0KK182 pKa = 10.56NYY184 pKa = 9.9YY185 pKa = 9.6SNKK188 pKa = 8.24QEE190 pKa = 4.1EE191 pKa = 4.58IGVFIHH197 pKa = 6.77NSGEE201 pKa = 4.24VPQITQLLQAIRR213 pKa = 11.84SRR215 pKa = 11.84GGTIEE220 pKa = 5.78LIFTSSTLPYY230 pKa = 10.38QGILVPLGSDD240 pKa = 3.23LTIQNLPGSATAEE253 pKa = 3.68MLRR256 pKa = 11.84FNDD259 pKa = 3.44TRR261 pKa = 11.84SGEE264 pKa = 4.1SGTWDD269 pKa = 3.21KK270 pKa = 11.23FFNNYY275 pKa = 6.58TTTLTPSGTRR285 pKa = 11.84VIQFLDD291 pKa = 3.3SKK293 pKa = 10.87YY294 pKa = 10.45EE295 pKa = 3.99NEE297 pKa = 4.32AGTGFEE303 pKa = 5.2GFIDD307 pKa = 4.02QNPQIIPNTAEE318 pKa = 3.67ITLKK322 pKa = 10.47EE323 pKa = 4.17FPLEE327 pKa = 4.56NIDD330 pKa = 4.12NMRR333 pKa = 11.84DD334 pKa = 3.37KK335 pKa = 10.91ILAQQGNIEE344 pKa = 4.26FEE346 pKa = 4.25LMEE349 pKa = 4.66NQPSPYY355 pKa = 10.16GDD357 pKa = 3.78PLLFYY362 pKa = 10.48PDD364 pKa = 3.81TNKK367 pKa = 10.55SNSFFNLEE375 pKa = 3.73GLAVKK380 pKa = 9.15TYY382 pKa = 10.71QSDD385 pKa = 3.92IFNNWINTEE394 pKa = 3.94WLDD397 pKa = 3.92GEE399 pKa = 4.68NGINTITAVDD409 pKa = 3.86VSSGFLNLDD418 pKa = 3.54SLNLAKK424 pKa = 10.25KK425 pKa = 10.31VYY427 pKa = 11.1DD428 pKa = 3.56MLNRR432 pKa = 11.84IAISGGSYY440 pKa = 10.56KK441 pKa = 10.71DD442 pKa = 3.14WLEE445 pKa = 4.18SVWMHH450 pKa = 6.14EE451 pKa = 4.46YY452 pKa = 10.27ISNAEE457 pKa = 4.07SPVYY461 pKa = 10.33EE462 pKa = 4.74GGLSKK467 pKa = 10.68EE468 pKa = 4.16IVFSEE473 pKa = 4.6VVSNSSGIGEE483 pKa = 4.49DD484 pKa = 4.47SSNQPLGTLAGRR496 pKa = 11.84GHH498 pKa = 7.83LSGKK502 pKa = 9.8HH503 pKa = 5.31KK504 pKa = 10.39GGHH507 pKa = 5.68IIIRR511 pKa = 11.84TNEE514 pKa = 3.53PSLIMGIVSITPRR527 pKa = 11.84IDD529 pKa = 3.26YY530 pKa = 10.52SQGNEE535 pKa = 3.52WFTNLDD541 pKa = 3.6TMDD544 pKa = 5.27DD545 pKa = 3.63LHH547 pKa = 8.3KK548 pKa = 10.67PGLDD552 pKa = 3.44GIGFQEE558 pKa = 4.75LVTEE562 pKa = 5.45QFAAWDD568 pKa = 4.34TVWDD572 pKa = 4.03TNEE575 pKa = 3.56DD576 pKa = 3.48RR577 pKa = 11.84VIKK580 pKa = 10.34FSAGKK585 pKa = 8.74QPAWINYY592 pKa = 4.33MTNYY596 pKa = 9.25NRR598 pKa = 11.84VYY600 pKa = 11.34GNFAKK605 pKa = 10.56KK606 pKa = 9.86NNEE609 pKa = 3.38MFMTLTRR616 pKa = 11.84RR617 pKa = 11.84FSYY620 pKa = 10.8NKK622 pKa = 9.5NAANNKK628 pKa = 9.57SAIEE632 pKa = 4.14DD633 pKa = 3.54LTTYY637 pKa = 8.45VQPDD641 pKa = 3.01KK642 pKa = 11.23HH643 pKa = 7.01QDD645 pKa = 2.87TFAITTIDD653 pKa = 3.42AQNFWTQIAVKK664 pKa = 9.89CFARR668 pKa = 11.84RR669 pKa = 11.84KK670 pKa = 8.28MSAKK674 pKa = 10.15IIPNLL679 pKa = 3.51

Molecular weight: 76.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V1FVW7|A0A4V1FVW7_9VIRU DNA pilot protein OS=Tortoise microvirus 14 OX=2583115 PE=4 SV=1
MM1 pKa = 7.66LKK3 pKa = 10.54NNSKK7 pKa = 9.83IKK9 pKa = 10.46NMKK12 pKa = 9.67ISINMEE18 pKa = 3.93TFTLQDD24 pKa = 3.68LKK26 pKa = 10.79ILQSILLRR34 pKa = 11.84YY35 pKa = 9.45KK36 pKa = 9.58STYY39 pKa = 9.24NRR41 pKa = 11.84NALKK45 pKa = 10.52NINKK49 pKa = 10.03LIVDD53 pKa = 5.01IINEE57 pKa = 4.13KK58 pKa = 10.5YY59 pKa = 10.68SIPFEE64 pKa = 4.49PDD66 pKa = 2.75PLFF69 pKa = 4.52

Molecular weight: 8.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1616

69

679

269.3

30.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.755 ± 1.538

0.433 ± 0.292

4.517 ± 0.398

7.611 ± 1.445

3.465 ± 0.746

6.869 ± 0.935

1.361 ± 0.264

8.478 ± 0.623

8.168 ± 2.199

7.611 ± 0.788

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.723 ± 0.428

7.735 ± 0.617

3.527 ± 0.677

4.703 ± 1.331

5.012 ± 0.792

6.559 ± 0.773

5.941 ± 0.786

3.527 ± 0.718

1.361 ± 0.283

4.641 ± 0.99
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski