Temnothorax longispinosus

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea; Formicidae;

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13035 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S2KMM1|A0A4S2KMM1_9HYME Uncharacterized protein OS=Temnothorax longispinosus OX=300112 GN=DBV15_11296 PE=4 SV=1
MM1 pKa = 7.38TSNPGRR7 pKa = 11.84TMTMYY12 pKa = 10.34DD13 pKa = 2.84IPGIVAKK20 pKa = 10.31ALPLAVTYY28 pKa = 10.09QNIVSGFEE36 pKa = 4.02CTGISPFNPDD46 pKa = 2.32IFQEE50 pKa = 4.49YY51 pKa = 10.26EE52 pKa = 3.83FLTNSVTDD60 pKa = 3.39RR61 pKa = 11.84EE62 pKa = 4.55YY63 pKa = 10.51PFTFVDD69 pKa = 3.34PDD71 pKa = 4.02KK72 pKa = 10.91IYY74 pKa = 11.4LNATDD79 pKa = 5.61SDD81 pKa = 4.02AADD84 pKa = 3.56QNEE87 pKa = 4.49DD88 pKa = 3.66KK89 pKa = 10.63VDD91 pKa = 3.75PMALSMSHH99 pKa = 6.78YY100 pKa = 10.7GDD102 pKa = 3.63TLSGEE107 pKa = 4.48LEE109 pKa = 4.28RR110 pKa = 11.84NDD112 pKa = 3.51GG113 pKa = 3.67

Molecular weight: 12.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S2L373|A0A4S2L373_9HYME Uncharacterized protein OS=Temnothorax longispinosus OX=300112 GN=DBV15_10873 PE=4 SV=1
MM1 pKa = 7.5EE2 pKa = 5.24KK3 pKa = 9.98KK4 pKa = 10.54KK5 pKa = 10.79KK6 pKa = 9.42IAAINGKK13 pKa = 9.64IGGAKK18 pKa = 9.61RR19 pKa = 11.84NRR21 pKa = 11.84GAVRR25 pKa = 11.84GRR27 pKa = 11.84GGFRR31 pKa = 11.84GRR33 pKa = 11.84GARR36 pKa = 11.84IKK38 pKa = 10.82RR39 pKa = 11.84NVGPILKK46 pKa = 9.43NQQQQNWQRR55 pKa = 11.84NANTASNARR64 pKa = 11.84GGFARR69 pKa = 11.84NRR71 pKa = 11.84FKK73 pKa = 11.2RR74 pKa = 11.84NGHH77 pKa = 5.85LARR80 pKa = 11.84SRR82 pKa = 11.84SNLSLNQKK90 pKa = 9.69ANNVRR95 pKa = 11.84GGAFNVRR102 pKa = 11.84RR103 pKa = 11.84GNRR106 pKa = 11.84GNRR109 pKa = 11.84FGLTRR114 pKa = 11.84SRR116 pKa = 11.84SRR118 pKa = 11.84TNLTFNQGGFFTNNKK133 pKa = 9.67SPLKK137 pKa = 9.55RR138 pKa = 11.84TSSLPNLRR146 pKa = 11.84DD147 pKa = 3.36PNSVHH152 pKa = 6.28NRR154 pKa = 11.84LGYY157 pKa = 10.01QSPAQIAYY165 pKa = 9.4RR166 pKa = 11.84NRR168 pKa = 11.84VKK170 pKa = 10.6RR171 pKa = 11.84AKK173 pKa = 10.04QLLLQRR179 pKa = 11.84QNQRR183 pKa = 11.84LNLQNQFRR191 pKa = 11.84MAQTGQKK198 pKa = 9.68IGLHH202 pKa = 5.57PMQPRR207 pKa = 11.84TLGRR211 pKa = 11.84QQILTSQRR219 pKa = 11.84RR220 pKa = 11.84FTTGGLL226 pKa = 3.47

Molecular weight: 25.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13004

31

13035

7195076

36

14793

552.0

62.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.484 ± 0.022

2.036 ± 0.031

5.457 ± 0.017

6.667 ± 0.034

3.529 ± 0.018

5.488 ± 0.027

2.5 ± 0.01

5.693 ± 0.025

6.198 ± 0.028

8.941 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.262 ± 0.011

4.903 ± 0.02

5.043 ± 0.027

4.045 ± 0.022

6.276 ± 0.024

8.103 ± 0.026

6.039 ± 0.018

6.11 ± 0.014

1.079 ± 0.007

3.108 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski