Amino acid dipepetide frequency for Pseudanabaena biceps PCC 7429

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.763AlaAla: 6.763 ± 0.092
0.785AlaCys: 0.785 ± 0.025
4.31AlaAsp: 4.31 ± 0.067
5.124AlaGlu: 5.124 ± 0.082
2.919AlaPhe: 2.919 ± 0.048
5.149AlaGly: 5.149 ± 0.073
1.314AlaHis: 1.314 ± 0.03
9.216AlaIle: 9.216 ± 0.097
4.859AlaLys: 4.859 ± 0.069
8.168AlaLeu: 8.168 ± 0.095
1.855AlaMet: 1.855 ± 0.042
4.162AlaAsn: 4.162 ± 0.078
2.992AlaPro: 2.992 ± 0.055
3.851AlaGln: 3.851 ± 0.058
3.302AlaArg: 3.302 ± 0.053
5.346AlaSer: 5.346 ± 0.066
4.605AlaThr: 4.605 ± 0.054
4.836AlaVal: 4.836 ± 0.067
1.006AlaTrp: 1.006 ± 0.027
2.259AlaTyr: 2.259 ± 0.042
0.0AlaXaa: 0.0 ± 0.0
Cys
0.661CysAla: 0.661 ± 0.02
0.129CysCys: 0.129 ± 0.01
0.724CysAsp: 0.724 ± 0.026
0.535CysGlu: 0.535 ± 0.02
0.408CysPhe: 0.408 ± 0.019
0.753CysGly: 0.753 ± 0.024
0.267CysHis: 0.267 ± 0.015
0.602CysIle: 0.602 ± 0.02
0.393CysLys: 0.393 ± 0.017
1.081CysLeu: 1.081 ± 0.03
0.173CysMet: 0.173 ± 0.011
0.405CysAsn: 0.405 ± 0.017
0.48CysPro: 0.48 ± 0.018
0.532CysGln: 0.532 ± 0.02
0.476CysArg: 0.476 ± 0.016
0.564CysSer: 0.564 ± 0.02
0.391CysThr: 0.391 ± 0.016
0.507CysVal: 0.507 ± 0.021
0.117CysTrp: 0.117 ± 0.008
0.325CysTyr: 0.325 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
3.963AspAla: 3.963 ± 0.061
0.564AspCys: 0.564 ± 0.02
2.531AspAsp: 2.531 ± 0.061
2.982AspGlu: 2.982 ± 0.054
2.448AspPhe: 2.448 ± 0.05
3.648AspGly: 3.648 ± 0.073
0.876AspHis: 0.876 ± 0.027
3.999AspIle: 3.999 ± 0.052
2.336AspLys: 2.336 ± 0.046
6.63AspLeu: 6.63 ± 0.084
0.897AspMet: 0.897 ± 0.022
2.105AspAsn: 2.105 ± 0.039
2.575AspPro: 2.575 ± 0.048
1.613AspGln: 1.613 ± 0.035
5.736AspArg: 5.736 ± 0.072
3.195AspSer: 3.195 ± 0.051
2.347AspThr: 2.347 ± 0.043
2.97AspVal: 2.97 ± 0.047
0.858AspTrp: 0.858 ± 0.027
1.859AspTyr: 1.859 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
5.368GluAla: 5.368 ± 0.08
0.473GluCys: 0.473 ± 0.021
2.788GluAsp: 2.788 ± 0.048
3.652GluGlu: 3.652 ± 0.055
2.466GluPhe: 2.466 ± 0.042
3.116GluGly: 3.116 ± 0.053
0.994GluHis: 0.994 ± 0.03
5.374GluIle: 5.374 ± 0.068
3.351GluLys: 3.351 ± 0.051
6.747GluLeu: 6.747 ± 0.083
1.388GluMet: 1.388 ± 0.031
2.679GluAsn: 2.679 ± 0.047
2.143GluPro: 2.143 ± 0.047
3.132GluGln: 3.132 ± 0.056
3.236GluArg: 3.236 ± 0.057
3.878GluSer: 3.878 ± 0.065
3.275GluThr: 3.275 ± 0.049
4.156GluVal: 4.156 ± 0.06
0.785GluTrp: 0.785 ± 0.023
1.865GluTyr: 1.865 ± 0.042
0.0GluXaa: 0.0 ± 0.0
Phe
3.41PheAla: 3.41 ± 0.055
0.515PheCys: 0.515 ± 0.018
2.456PheAsp: 2.456 ± 0.047
2.213PheGlu: 2.213 ± 0.042
1.598PhePhe: 1.598 ± 0.04
2.919PheGly: 2.919 ± 0.052
0.754PheHis: 0.754 ± 0.023
2.322PheIle: 2.322 ± 0.038
1.702PheLys: 1.702 ± 0.037
4.133PheLeu: 4.133 ± 0.049
0.783PheMet: 0.783 ± 0.021
1.67PheAsn: 1.67 ± 0.038
1.804PhePro: 1.804 ± 0.037
1.748PheGln: 1.748 ± 0.036
1.881PheArg: 1.881 ± 0.037
3.0PheSer: 3.0 ± 0.055
2.432PheThr: 2.432 ± 0.043
2.537PheVal: 2.537 ± 0.051
0.667PheTrp: 0.667 ± 0.022
1.319PheTyr: 1.319 ± 0.038
0.0PheXaa: 0.0 ± 0.0
Gly
4.907GlyAla: 4.907 ± 0.079
0.698GlyCys: 0.698 ± 0.022
3.741GlyAsp: 3.741 ± 0.061
3.661GlyGlu: 3.661 ± 0.056
2.94GlyPhe: 2.94 ± 0.045
4.482GlyGly: 4.482 ± 0.097
1.116GlyHis: 1.116 ± 0.034
5.036GlyIle: 5.036 ± 0.067
4.383GlyLys: 4.383 ± 0.061
6.586GlyLeu: 6.586 ± 0.072
1.494GlyMet: 1.494 ± 0.032
3.268GlyAsn: 3.268 ± 0.091
0.755GlyPro: 0.755 ± 0.025
2.621GlyGln: 2.621 ± 0.051
2.988GlyArg: 2.988 ± 0.051
4.176GlySer: 4.176 ± 0.065
3.612GlyThr: 3.612 ± 0.072
4.048GlyVal: 4.048 ± 0.06
1.0GlyTrp: 1.0 ± 0.031
2.318GlyTyr: 2.318 ± 0.042
0.0GlyXaa: 0.0 ± 0.0
His
1.135HisAla: 1.135 ± 0.026
0.253HisCys: 0.253 ± 0.013
0.878HisAsp: 0.878 ± 0.028
0.968HisGlu: 0.968 ± 0.023
0.804HisPhe: 0.804 ± 0.026
1.117HisGly: 1.117 ± 0.029
0.583HisHis: 0.583 ± 0.02
1.156HisIle: 1.156 ± 0.027
0.789HisLys: 0.789 ± 0.026
2.202HisLeu: 2.202 ± 0.042
0.261HisMet: 0.261 ± 0.014
0.744HisAsn: 0.744 ± 0.021
1.214HisPro: 1.214 ± 0.03
1.057HisGln: 1.057 ± 0.029
1.126HisArg: 1.126 ± 0.032
1.139HisSer: 1.139 ± 0.031
0.929HisThr: 0.929 ± 0.025
0.866HisVal: 0.866 ± 0.026
0.302HisTrp: 0.302 ± 0.014
0.608HisTyr: 0.608 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
9.167IleAla: 9.167 ± 0.081
0.8IleCys: 0.8 ± 0.026
4.574IleAsp: 4.574 ± 0.059
4.585IleGlu: 4.585 ± 0.065
2.881IlePhe: 2.881 ± 0.056
4.939IleGly: 4.939 ± 0.063
1.337IleHis: 1.337 ± 0.033
3.727IleIle: 3.727 ± 0.057
3.305IleLys: 3.305 ± 0.055
6.976IleLeu: 6.976 ± 0.08
1.022IleMet: 1.022 ± 0.027
3.201IleAsn: 3.201 ± 0.05
3.577IlePro: 3.577 ± 0.051
3.012IleGln: 3.012 ± 0.053
3.226IleArg: 3.226 ± 0.054
5.293IleSer: 5.293 ± 0.074
3.919IleThr: 3.919 ± 0.052
4.718IleVal: 4.718 ± 0.054
0.862IleTrp: 0.862 ± 0.027
2.14IleTyr: 2.14 ± 0.034
0.0IleXaa: 0.0 ± 0.0
Lys
4.216LysAla: 4.216 ± 0.061
0.337LysCys: 0.337 ± 0.014
2.556LysAsp: 2.556 ± 0.052
3.017LysGlu: 3.017 ± 0.048
2.071LysPhe: 2.071 ± 0.042
2.943LysGly: 2.943 ± 0.048
0.876LysHis: 0.876 ± 0.028
3.831LysIle: 3.831 ± 0.054
2.484LysLys: 2.484 ± 0.051
5.606LysLeu: 5.606 ± 0.073
1.048LysMet: 1.048 ± 0.026
2.225LysAsn: 2.225 ± 0.046
2.446LysPro: 2.446 ± 0.044
2.659LysGln: 2.659 ± 0.048
2.51LysArg: 2.51 ± 0.047
3.524LysSer: 3.524 ± 0.056
2.846LysThr: 2.846 ± 0.046
3.321LysVal: 3.321 ± 0.053
0.567LysTrp: 0.567 ± 0.022
1.63LysTyr: 1.63 ± 0.037
0.0LysXaa: 0.0 ± 0.0
Leu
9.403LeuAla: 9.403 ± 0.095
1.017LeuCys: 1.017 ± 0.026
5.742LeuAsp: 5.742 ± 0.072
7.126LeuGlu: 7.126 ± 0.097
3.756LeuPhe: 3.756 ± 0.05
7.289LeuGly: 7.289 ± 0.073
1.9LeuHis: 1.9 ± 0.036
6.79LeuIle: 6.79 ± 0.076
5.451LeuLys: 5.451 ± 0.077
10.612LeuLeu: 10.612 ± 0.128
2.132LeuMet: 2.132 ± 0.038
4.698LeuAsn: 4.698 ± 0.063
5.399LeuPro: 5.399 ± 0.061
5.381LeuGln: 5.381 ± 0.076
5.559LeuArg: 5.559 ± 0.077
7.938LeuSer: 7.938 ± 0.096
6.0LeuThr: 6.0 ± 0.075
6.766LeuVal: 6.766 ± 0.069
1.297LeuTrp: 1.297 ± 0.038
2.748LeuTyr: 2.748 ± 0.046
0.0LeuXaa: 0.0 ± 0.0
Met
1.706MetAla: 1.706 ± 0.032
0.127MetCys: 0.127 ± 0.008
0.868MetAsp: 0.868 ± 0.022
1.08MetGlu: 1.08 ± 0.026
0.608MetPhe: 0.608 ± 0.022
1.463MetGly: 1.463 ± 0.034
0.329MetHis: 0.329 ± 0.017
1.171MetIle: 1.171 ± 0.025
0.911MetLys: 0.911 ± 0.026
2.067MetLeu: 2.067 ± 0.043
0.483MetMet: 0.483 ± 0.019
0.833MetAsn: 0.833 ± 0.024
1.033MetPro: 1.033 ± 0.028
1.094MetGln: 1.094 ± 0.024
1.103MetArg: 1.103 ± 0.029
1.601MetSer: 1.601 ± 0.033
1.386MetThr: 1.386 ± 0.027
1.244MetVal: 1.244 ± 0.029
0.189MetTrp: 0.189 ± 0.013
0.403MetTyr: 0.403 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
3.565AsnAla: 3.565 ± 0.054
0.465AsnCys: 0.465 ± 0.017
2.034AsnAsp: 2.034 ± 0.047
2.05AsnGlu: 2.05 ± 0.04
2.036AsnPhe: 2.036 ± 0.04
3.048AsnGly: 3.048 ± 0.078
0.884AsnHis: 0.884 ± 0.024
3.24AsnIle: 3.24 ± 0.067
1.69AsnLys: 1.69 ± 0.041
5.97AsnLeu: 5.97 ± 0.097
0.742AsnMet: 0.742 ± 0.023
2.127AsnAsn: 2.127 ± 0.054
2.811AsnPro: 2.811 ± 0.049
2.437AsnGln: 2.437 ± 0.047
2.311AsnArg: 2.311 ± 0.047
3.375AsnSer: 3.375 ± 0.076
2.271AsnThr: 2.271 ± 0.052
2.496AsnVal: 2.496 ± 0.048
0.656AsnTrp: 0.656 ± 0.021
1.486AsnTyr: 1.486 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
2.992ProAla: 2.992 ± 0.044
0.332ProCys: 0.332 ± 0.013
2.952ProAsp: 2.952 ± 0.052
3.557ProGlu: 3.557 ± 0.054
1.711ProPhe: 1.711 ± 0.036
1.106ProGly: 1.106 ± 0.033
0.916ProHis: 0.916 ± 0.029
3.531ProIle: 3.531 ± 0.049
2.543ProLys: 2.543 ± 0.052
4.284ProLeu: 4.284 ± 0.059
0.842ProMet: 0.842 ± 0.025
2.49ProAsn: 2.49 ± 0.045
1.932ProPro: 1.932 ± 0.05
2.3ProGln: 2.3 ± 0.045
1.626ProArg: 1.626 ± 0.033
3.319ProSer: 3.319 ± 0.061
2.712ProThr: 2.712 ± 0.054
2.824ProVal: 2.824 ± 0.047
0.541ProTrp: 0.541 ± 0.02
1.295ProTyr: 1.295 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
4.038GlnAla: 4.038 ± 0.073
0.354GlnCys: 0.354 ± 0.019
2.181GlnAsp: 2.181 ± 0.038
3.145GlnGlu: 3.145 ± 0.049
1.765GlnPhe: 1.765 ± 0.038
2.856GlnGly: 2.856 ± 0.043
0.738GlnHis: 0.738 ± 0.025
4.007GlnIle: 4.007 ± 0.051
2.866GlnLys: 2.866 ± 0.051
5.005GlnLeu: 5.005 ± 0.062
1.033GlnMet: 1.033 ± 0.027
2.213GlnAsn: 2.213 ± 0.042
2.127GlnPro: 2.127 ± 0.043
2.834GlnGln: 2.834 ± 0.06
2.54GlnArg: 2.54 ± 0.043
3.279GlnSer: 3.279 ± 0.047
2.761GlnThr: 2.761 ± 0.043
3.431GlnVal: 3.431 ± 0.046
0.677GlnTrp: 0.677 ± 0.022
1.246GlnTyr: 1.246 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
3.493ArgAla: 3.493 ± 0.059
0.484ArgCys: 0.484 ± 0.018
3.137ArgAsp: 3.137 ± 0.051
3.321ArgGlu: 3.321 ± 0.049
2.22ArgPhe: 2.22 ± 0.036
2.816ArgGly: 2.816 ± 0.051
1.049ArgHis: 1.049 ± 0.029
3.66ArgIle: 3.66 ± 0.056
2.493ArgLys: 2.493 ± 0.049
5.896ArgLeu: 5.896 ± 0.071
1.061ArgMet: 1.061 ± 0.027
2.162ArgAsn: 2.162 ± 0.036
1.921ArgPro: 1.921 ± 0.038
3.053ArgGln: 3.053 ± 0.049
2.686ArgArg: 2.686 ± 0.049
3.92ArgSer: 3.92 ± 0.063
2.434ArgThr: 2.434 ± 0.047
3.227ArgVal: 3.227 ± 0.049
0.718ArgTrp: 0.718 ± 0.022
1.868ArgTyr: 1.868 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
4.883SerAla: 4.883 ± 0.058
0.61SerCys: 0.61 ± 0.023
4.153SerAsp: 4.153 ± 0.053
4.115SerGlu: 4.115 ± 0.066
2.806SerPhe: 2.806 ± 0.049
4.825SerGly: 4.825 ± 0.078
1.356SerHis: 1.356 ± 0.038
4.486SerIle: 4.486 ± 0.057
3.248SerLys: 3.248 ± 0.049
7.831SerLeu: 7.831 ± 0.096
1.349SerMet: 1.349 ± 0.029
3.33SerAsn: 3.33 ± 0.075
3.63SerPro: 3.63 ± 0.055
3.872SerGln: 3.872 ± 0.052
3.245SerArg: 3.245 ± 0.049
5.11SerSer: 5.11 ± 0.091
3.735SerThr: 3.735 ± 0.067
3.93SerVal: 3.93 ± 0.05
0.861SerTrp: 0.861 ± 0.027
2.133SerTyr: 2.133 ± 0.048
0.0SerXaa: 0.0 ± 0.0
Thr
4.558ThrAla: 4.558 ± 0.067
0.468ThrCys: 0.468 ± 0.02
2.923ThrAsp: 2.923 ± 0.048
3.205ThrGlu: 3.205 ± 0.052
2.113ThrPhe: 2.113 ± 0.042
4.105ThrGly: 4.105 ± 0.082
0.983ThrHis: 0.983 ± 0.026
4.096ThrIle: 4.096 ± 0.061
2.683ThrLys: 2.683 ± 0.043
5.666ThrLeu: 5.666 ± 0.06
0.913ThrMet: 0.913 ± 0.025
2.636ThrAsn: 2.636 ± 0.058
2.793ThrPro: 2.793 ± 0.049
2.365ThrGln: 2.365 ± 0.04
2.198ThrArg: 2.198 ± 0.036
3.774ThrSer: 3.774 ± 0.068
3.265ThrThr: 3.265 ± 0.079
3.642ThrVal: 3.642 ± 0.061
0.7ThrTrp: 0.7 ± 0.023
1.656ThrTyr: 1.656 ± 0.051
0.0ThrXaa: 0.0 ± 0.0
Val
5.382ValAla: 5.382 ± 0.07
0.616ValCys: 0.616 ± 0.023
3.285ValAsp: 3.285 ± 0.052
3.847ValGlu: 3.847 ± 0.063
2.379ValPhe: 2.379 ± 0.043
4.275ValGly: 4.275 ± 0.055
0.926ValHis: 0.926 ± 0.022
4.349ValIle: 4.349 ± 0.06
3.335ValLys: 3.335 ± 0.05
6.285ValLeu: 6.285 ± 0.075
1.446ValMet: 1.446 ± 0.029
2.986ValAsn: 2.986 ± 0.052
2.576ValPro: 2.576 ± 0.048
2.498ValGln: 2.498 ± 0.04
3.142ValArg: 3.142 ± 0.051
4.379ValSer: 4.379 ± 0.068
3.76ValThr: 3.76 ± 0.066
4.174ValVal: 4.174 ± 0.068
0.783ValTrp: 0.783 ± 0.026
1.734ValTyr: 1.734 ± 0.04
0.0ValXaa: 0.0 ± 0.0
Trp
0.864TrpAla: 0.864 ± 0.027
0.139TrpCys: 0.139 ± 0.009
0.614TrpAsp: 0.614 ± 0.021
0.888TrpGlu: 0.888 ± 0.027
0.577TrpPhe: 0.577 ± 0.019
0.856TrpGly: 0.856 ± 0.024
0.323TrpHis: 0.323 ± 0.015
0.87TrpIle: 0.87 ± 0.026
0.603TrpLys: 0.603 ± 0.02
1.749TrpLeu: 1.749 ± 0.04
0.326TrpMet: 0.326 ± 0.014
0.594TrpAsn: 0.594 ± 0.02
0.109TrpPro: 0.109 ± 0.009
1.213TrpGln: 1.213 ± 0.03
0.793TrpArg: 0.793 ± 0.026
0.782TrpSer: 0.782 ± 0.028
0.598TrpThr: 0.598 ± 0.022
0.781TrpVal: 0.781 ± 0.025
0.237TrpTrp: 0.237 ± 0.014
0.377TrpTyr: 0.377 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.347TyrAla: 2.347 ± 0.039
0.356TyrCys: 0.356 ± 0.017
1.685TyrAsp: 1.685 ± 0.059
1.798TyrGlu: 1.798 ± 0.041
1.357TyrPhe: 1.357 ± 0.034
2.127TyrGly: 2.127 ± 0.043
0.61TyrHis: 0.61 ± 0.023
1.8TyrIle: 1.8 ± 0.045
1.288TyrLys: 1.288 ± 0.031
3.511TyrLeu: 3.511 ± 0.051
0.45TyrMet: 0.45 ± 0.016
1.165TyrAsn: 1.165 ± 0.033
1.486TyrPro: 1.486 ± 0.032
1.823TyrGln: 1.823 ± 0.037
1.934TyrArg: 1.934 ± 0.04
1.914TyrSer: 1.914 ± 0.048
1.466TyrThr: 1.466 ± 0.039
1.683TyrVal: 1.683 ± 0.04
0.473TyrTrp: 0.473 ± 0.018
0.968TyrTyr: 0.968 ± 0.033
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4751 proteins (1492453 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski