Amino acid dipepetide frequency for Sphingomonas sp. Ant20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.452AlaAla: 20.452 ± 0.242	1.139AlaCys: 1.139 ± 0.044	8.165AlaAsp: 8.165 ± 0.109	7.212AlaGlu: 7.212 ± 0.116	4.46AlaPhe: 4.46 ± 0.08	11.544AlaGly: 11.544 ± 0.136	2.33AlaHis: 2.33 ± 0.064	7.389AlaIle: 7.389 ± 0.112	4.249AlaLys: 4.249 ± 0.092	13.897AlaLeu: 13.897 ± 0.156	4.164AlaMet: 4.164 ± 0.076	3.13AlaAsn: 3.13 ± 0.08	6.093AlaPro: 6.093 ± 0.09	4.597AlaGln: 4.597 ± 0.1	9.966AlaArg: 9.966 ± 0.123	6.833AlaSer: 6.833 ± 0.105	7.863AlaThr: 7.863 ± 0.09	9.377AlaVal: 9.377 ± 0.122	1.697AlaTrp: 1.697 ± 0.044	2.632AlaTyr: 2.632 ± 0.063	0.0AlaXaa: 0.0 ± 0.0
Cys	1.009CysAla: 1.009 ± 0.042	0.1CysCys: 0.1 ± 0.012	0.536CysAsp: 0.536 ± 0.026	0.358CysGlu: 0.358 ± 0.024	0.29CysPhe: 0.29 ± 0.021	0.841CysGly: 0.841 ± 0.039	0.194CysHis: 0.194 ± 0.018	0.338CysIle: 0.338 ± 0.024	0.144CysLys: 0.144 ± 0.013	0.653CysLeu: 0.653 ± 0.027	0.14CysMet: 0.14 ± 0.014	0.193CysAsn: 0.193 ± 0.014	0.482CysPro: 0.482 ± 0.03	0.18CysGln: 0.18 ± 0.016	0.603CysArg: 0.603 ± 0.033	0.451CysSer: 0.451 ± 0.024	0.406CysThr: 0.406 ± 0.02	0.573CysVal: 0.573 ± 0.029	0.108CysTrp: 0.108 ± 0.012	0.147CysTyr: 0.147 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	8.671AspAla: 8.671 ± 0.126	0.448AspCys: 0.448 ± 0.027	3.717AspAsp: 3.717 ± 0.071	3.265AspGlu: 3.265 ± 0.068	2.039AspPhe: 2.039 ± 0.053	5.669AspGly: 5.669 ± 0.092	1.382AspHis: 1.382 ± 0.042	2.758AspIle: 2.758 ± 0.053	1.565AspLys: 1.565 ± 0.054	5.784AspLeu: 5.784 ± 0.085	1.408AspMet: 1.408 ± 0.045	1.182AspAsn: 1.182 ± 0.043	3.844AspPro: 3.844 ± 0.066	1.931AspGln: 1.931 ± 0.047	5.451AspArg: 5.451 ± 0.088	2.258AspSer: 2.258 ± 0.061	3.231AspThr: 3.231 ± 0.069	4.373AspVal: 4.373 ± 0.078	1.139AspTrp: 1.139 ± 0.04	1.501AspTyr: 1.501 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	7.137GluAla: 7.137 ± 0.11	0.276GluCys: 0.276 ± 0.019	2.471GluAsp: 2.471 ± 0.065	2.29GluGlu: 2.29 ± 0.06	1.352GluPhe: 1.352 ± 0.04	3.984GluGly: 3.984 ± 0.079	1.058GluHis: 1.058 ± 0.044	2.643GluIle: 2.643 ± 0.053	1.636GluLys: 1.636 ± 0.05	4.568GluLeu: 4.568 ± 0.077	1.241GluMet: 1.241 ± 0.043	1.102GluAsn: 1.102 ± 0.035	2.447GluPro: 2.447 ± 0.065	2.006GluGln: 2.006 ± 0.05	4.991GluArg: 4.991 ± 0.088	2.087GluSer: 2.087 ± 0.048	3.314GluThr: 3.314 ± 0.058	3.205GluVal: 3.205 ± 0.069	0.649GluTrp: 0.649 ± 0.031	0.858GluTyr: 0.858 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	4.939PheAla: 4.939 ± 0.082	0.315PheCys: 0.315 ± 0.02	2.707PheAsp: 2.707 ± 0.058	1.816PheGlu: 1.816 ± 0.045	1.183PhePhe: 1.183 ± 0.044	3.554PheGly: 3.554 ± 0.069	0.661PheHis: 0.661 ± 0.026	1.258PheIle: 1.258 ± 0.041	0.89PheLys: 0.89 ± 0.032	2.962PheLeu: 2.962 ± 0.076	0.69PheMet: 0.69 ± 0.031	0.938PheAsn: 0.938 ± 0.038	1.309PhePro: 1.309 ± 0.043	0.788PheGln: 0.788 ± 0.032	2.227PheArg: 2.227 ± 0.054	1.848PheSer: 1.848 ± 0.049	2.02PheThr: 2.02 ± 0.055	2.669PheVal: 2.669 ± 0.063	0.511PheTrp: 0.511 ± 0.026	0.82PheTyr: 0.82 ± 0.038	0.0PheXaa: 0.0 ± 0.0
Gly	9.922GlyAla: 9.922 ± 0.109	0.819GlyCys: 0.819 ± 0.035	5.197GlyAsp: 5.197 ± 0.078	4.429GlyGlu: 4.429 ± 0.079	3.637GlyPhe: 3.637 ± 0.076	7.986GlyGly: 7.986 ± 0.14	1.858GlyHis: 1.858 ± 0.044	4.591GlyIle: 4.591 ± 0.078	3.0GlyLys: 3.0 ± 0.068	8.3GlyLeu: 8.3 ± 0.112	2.41GlyMet: 2.41 ± 0.059	2.068GlyAsn: 2.068 ± 0.05	3.382GlyPro: 3.382 ± 0.068	2.905GlyGln: 2.905 ± 0.066	6.628GlyArg: 6.628 ± 0.101	4.913GlySer: 4.913 ± 0.09	5.205GlyThr: 5.205 ± 0.097	6.47GlyVal: 6.47 ± 0.092	1.666GlyTrp: 1.666 ± 0.044	2.416GlyTyr: 2.416 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	2.668HisAla: 2.668 ± 0.07	0.215HisCys: 0.215 ± 0.017	1.354HisAsp: 1.354 ± 0.049	0.972HisGlu: 0.972 ± 0.04	0.715HisPhe: 0.715 ± 0.031	1.809HisGly: 1.809 ± 0.048	0.556HisHis: 0.556 ± 0.035	0.863HisIle: 0.863 ± 0.031	0.404HisLys: 0.404 ± 0.026	1.796HisLeu: 1.796 ± 0.043	0.401HisMet: 0.401 ± 0.023	0.404HisAsn: 0.404 ± 0.023	1.232HisPro: 1.232 ± 0.038	0.541HisGln: 0.541 ± 0.03	1.643HisArg: 1.643 ± 0.045	0.826HisSer: 0.826 ± 0.032	0.741HisThr: 0.741 ± 0.03	1.553HisVal: 1.553 ± 0.044	0.315HisTrp: 0.315 ± 0.02	0.515HisTyr: 0.515 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	8.344IleAla: 8.344 ± 0.126	0.404IleCys: 0.404 ± 0.023	4.133IleAsp: 4.133 ± 0.083	3.333IleGlu: 3.333 ± 0.056	1.383IlePhe: 1.383 ± 0.046	5.35IleGly: 5.35 ± 0.087	0.804IleHis: 0.804 ± 0.035	1.83IleIle: 1.83 ± 0.056	1.163IleLys: 1.163 ± 0.036	4.108IleLeu: 4.108 ± 0.081	0.892IleMet: 0.892 ± 0.034	1.19IleAsn: 1.19 ± 0.042	2.219IlePro: 2.219 ± 0.052	1.116IleGln: 1.116 ± 0.036	3.266IleArg: 3.266 ± 0.06	2.302IleSer: 2.302 ± 0.056	2.71IleThr: 2.71 ± 0.058	4.506IleVal: 4.506 ± 0.081	0.542IleTrp: 0.542 ± 0.025	0.908IleTyr: 0.908 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	3.993LysAla: 3.993 ± 0.082	0.137LysCys: 0.137 ± 0.012	1.476LysAsp: 1.476 ± 0.047	0.968LysGlu: 0.968 ± 0.039	0.794LysPhe: 0.794 ± 0.034	2.523LysGly: 2.523 ± 0.061	0.499LysHis: 0.499 ± 0.025	1.423LysIle: 1.423 ± 0.046	0.995LysLys: 0.995 ± 0.05	2.976LysLeu: 2.976 ± 0.062	0.768LysMet: 0.768 ± 0.033	0.669LysAsn: 0.669 ± 0.031	2.061LysPro: 2.061 ± 0.055	0.877LysGln: 0.877 ± 0.034	2.358LysArg: 2.358 ± 0.058	1.479LysSer: 1.479 ± 0.046	1.927LysThr: 1.927 ± 0.05	2.104LysVal: 2.104 ± 0.062	0.342LysTrp: 0.342 ± 0.02	0.552LysTyr: 0.552 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	13.942LeuAla: 13.942 ± 0.162	0.754LeuCys: 0.754 ± 0.03	6.127LeuAsp: 6.127 ± 0.092	4.239LeuGlu: 4.239 ± 0.075	3.395LeuPhe: 3.395 ± 0.079	8.338LeuGly: 8.338 ± 0.105	1.859LeuHis: 1.859 ± 0.053	4.727LeuIle: 4.727 ± 0.074	2.892LeuLys: 2.892 ± 0.072	9.022LeuLeu: 9.022 ± 0.138	2.074LeuMet: 2.074 ± 0.051	2.305LeuAsn: 2.305 ± 0.059	5.356LeuPro: 5.356 ± 0.092	2.465LeuGln: 2.465 ± 0.053	7.038LeuArg: 7.038 ± 0.101	5.635LeuSer: 5.635 ± 0.086	5.695LeuThr: 5.695 ± 0.085	7.379LeuVal: 7.379 ± 0.113	1.185LeuTrp: 1.185 ± 0.042	1.911LeuTyr: 1.911 ± 0.052	0.0LeuXaa: 0.0 ± 0.0
Met	3.346MetAla: 3.346 ± 0.063	0.142MetCys: 0.142 ± 0.015	1.058MetAsp: 1.058 ± 0.038	0.931MetGlu: 0.931 ± 0.036	0.713MetPhe: 0.713 ± 0.034	1.743MetGly: 1.743 ± 0.05	0.446MetHis: 0.446 ± 0.025	1.502MetIle: 1.502 ± 0.039	0.956MetLys: 0.956 ± 0.036	2.714MetLeu: 2.714 ± 0.061	0.659MetMet: 0.659 ± 0.033	0.649MetAsn: 0.649 ± 0.027	1.59MetPro: 1.59 ± 0.045	0.755MetGln: 0.755 ± 0.031	1.852MetArg: 1.852 ± 0.048	1.404MetSer: 1.404 ± 0.041	2.159MetThr: 2.159 ± 0.057	1.634MetVal: 1.634 ± 0.044	0.173MetTrp: 0.173 ± 0.014	0.269MetTyr: 0.269 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.185AsnAla: 3.185 ± 0.072	0.234AsnCys: 0.234 ± 0.017	1.435AsnAsp: 1.435 ± 0.045	1.025AsnGlu: 1.025 ± 0.042	0.815AsnPhe: 0.815 ± 0.038	2.328AsnGly: 2.328 ± 0.077	0.445AsnHis: 0.445 ± 0.025	1.198AsnIle: 1.198 ± 0.036	0.634AsnLys: 0.634 ± 0.029	2.238AsnLeu: 2.238 ± 0.055	0.482AsnMet: 0.482 ± 0.026	0.562AsnAsn: 0.562 ± 0.033	1.554AsnPro: 1.554 ± 0.049	0.712AsnGln: 0.712 ± 0.037	1.766AsnArg: 1.766 ± 0.047	1.103AsnSer: 1.103 ± 0.042	1.253AsnThr: 1.253 ± 0.049	1.755AsnVal: 1.755 ± 0.05	0.374AsnTrp: 0.374 ± 0.02	0.654AsnTyr: 0.654 ± 0.037	0.0AsnXaa: 0.0 ± 0.0
Pro	7.218ProAla: 7.218 ± 0.103	0.336ProCys: 0.336 ± 0.019	3.733ProAsp: 3.733 ± 0.076	2.971ProGlu: 2.971 ± 0.07	1.848ProPhe: 1.848 ± 0.042	4.642ProGly: 4.642 ± 0.094	1.009ProHis: 1.009 ± 0.04	2.593ProIle: 2.593 ± 0.061	1.51ProLys: 1.51 ± 0.047	4.587ProLeu: 4.587 ± 0.073	1.279ProMet: 1.279 ± 0.043	1.191ProAsn: 1.191 ± 0.04	2.512ProPro: 2.512 ± 0.074	1.511ProGln: 1.511 ± 0.048	3.219ProArg: 3.219 ± 0.071	2.717ProSer: 2.717 ± 0.064	2.886ProThr: 2.886 ± 0.062	4.333ProVal: 4.333 ± 0.079	0.64ProTrp: 0.64 ± 0.027	1.042ProTyr: 1.042 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	4.311GlnAla: 4.311 ± 0.084	0.206GlnCys: 0.206 ± 0.017	1.412GlnAsp: 1.412 ± 0.042	1.107GlnGlu: 1.107 ± 0.04	0.982GlnPhe: 0.982 ± 0.039	2.358GlnGly: 2.358 ± 0.061	0.564GlnHis: 0.564 ± 0.029	1.75GlnIle: 1.75 ± 0.045	0.804GlnLys: 0.804 ± 0.027	2.933GlnLeu: 2.933 ± 0.063	0.819GlnMet: 0.819 ± 0.031	0.723GlnAsn: 0.723 ± 0.032	1.689GlnPro: 1.689 ± 0.045	1.172GlnGln: 1.172 ± 0.048	2.593GlnArg: 2.593 ± 0.064	1.782GlnSer: 1.782 ± 0.045	1.858GlnThr: 1.858 ± 0.052	2.24GlnVal: 2.24 ± 0.059	0.395GlnTrp: 0.395 ± 0.024	0.564GlnTyr: 0.564 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	9.225ArgAla: 9.225 ± 0.146	0.559ArgCys: 0.559 ± 0.031	4.725ArgAsp: 4.725 ± 0.088	3.85ArgGlu: 3.85 ± 0.074	3.074ArgPhe: 3.074 ± 0.065	5.309ArgGly: 5.309 ± 0.092	1.802ArgHis: 1.802 ± 0.06	4.285ArgIle: 4.285 ± 0.074	2.088ArgLys: 2.088 ± 0.053	8.033ArgLeu: 8.033 ± 0.121	2.213ArgMet: 2.213 ± 0.048	1.778ArgAsn: 1.778 ± 0.051	3.766ArgPro: 3.766 ± 0.07	2.533ArgGln: 2.533 ± 0.059	6.311ArgArg: 6.311 ± 0.104	3.999ArgSer: 3.999 ± 0.074	4.264ArgThr: 4.264 ± 0.073	5.376ArgVal: 5.376 ± 0.097	1.229ArgTrp: 1.229 ± 0.039	1.895ArgTyr: 1.895 ± 0.056	0.0ArgXaa: 0.0 ± 0.0
Ser	6.363SerAla: 6.363 ± 0.107	0.425SerCys: 0.425 ± 0.023	3.079SerAsp: 3.079 ± 0.057	2.273SerGlu: 2.273 ± 0.053	2.02SerPhe: 2.02 ± 0.047	5.29SerGly: 5.29 ± 0.091	0.924SerHis: 0.924 ± 0.032	2.712SerIle: 2.712 ± 0.064	1.388SerLys: 1.388 ± 0.044	4.928SerLeu: 4.928 ± 0.079	1.225SerMet: 1.225 ± 0.041	1.447SerAsn: 1.447 ± 0.045	2.809SerPro: 2.809 ± 0.075	1.439SerGln: 1.439 ± 0.047	3.652SerArg: 3.652 ± 0.08	2.777SerSer: 2.777 ± 0.084	2.936SerThr: 2.936 ± 0.07	3.973SerVal: 3.973 ± 0.073	0.72SerTrp: 0.72 ± 0.026	1.268SerTyr: 1.268 ± 0.045	0.0SerXaa: 0.0 ± 0.0
Thr	7.493ThrAla: 7.493 ± 0.109	0.373ThrCys: 0.373 ± 0.019	3.281ThrAsp: 3.281 ± 0.07	2.344ThrGlu: 2.344 ± 0.059	1.759ThrPhe: 1.759 ± 0.05	5.838ThrGly: 5.838 ± 0.093	0.967ThrHis: 0.967 ± 0.038	3.515ThrIle: 3.515 ± 0.064	1.534ThrLys: 1.534 ± 0.041	6.482ThrLeu: 6.482 ± 0.093	1.395ThrMet: 1.395 ± 0.04	1.387ThrAsn: 1.387 ± 0.05	3.749ThrPro: 3.749 ± 0.07	1.595ThrGln: 1.595 ± 0.042	4.134ThrArg: 4.134 ± 0.081	3.028ThrSer: 3.028 ± 0.074	3.47ThrThr: 3.47 ± 0.075	4.607ThrVal: 4.607 ± 0.083	0.651ThrTrp: 0.651 ± 0.027	1.237ThrTyr: 1.237 ± 0.044	0.0ThrXaa: 0.0 ± 0.0
Val	10.727ValAla: 10.727 ± 0.135	0.521ValCys: 0.521 ± 0.025	4.737ValAsp: 4.737 ± 0.078	4.408ValGlu: 4.408 ± 0.083	2.332ValPhe: 2.332 ± 0.059	5.69ValGly: 5.69 ± 0.084	1.361ValHis: 1.361 ± 0.037	3.681ValIle: 3.681 ± 0.071	2.097ValLys: 2.097 ± 0.065	6.761ValLeu: 6.761 ± 0.103	1.718ValMet: 1.718 ± 0.046	1.858ValAsn: 1.858 ± 0.055	3.914ValPro: 3.914 ± 0.072	1.98ValGln: 1.98 ± 0.053	5.446ValArg: 5.446 ± 0.084	4.153ValSer: 4.153 ± 0.079	4.955ValThr: 4.955 ± 0.082	5.884ValVal: 5.884 ± 0.104	0.892ValTrp: 0.892 ± 0.03	1.362ValTyr: 1.362 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	1.383TrpAla: 1.383 ± 0.038	0.13TrpCys: 0.13 ± 0.014	0.702TrpAsp: 0.702 ± 0.031	0.483TrpGlu: 0.483 ± 0.026	0.542TrpPhe: 0.542 ± 0.024	0.894TrpGly: 0.894 ± 0.029	0.358TrpHis: 0.358 ± 0.022	0.635TrpIle: 0.635 ± 0.033	0.463TrpLys: 0.463 ± 0.025	1.63TrpLeu: 1.63 ± 0.055	0.323TrpMet: 0.323 ± 0.019	0.42TrpAsn: 0.42 ± 0.025	0.749TrpPro: 0.749 ± 0.031	0.53TrpGln: 0.53 ± 0.025	1.293TrpArg: 1.293 ± 0.045	0.931TrpSer: 0.931 ± 0.036	0.944TrpThr: 0.944 ± 0.037	0.861TrpVal: 0.861 ± 0.031	0.254TrpTrp: 0.254 ± 0.019	0.275TrpTyr: 0.275 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.86TyrAla: 2.86 ± 0.063	0.213TyrCys: 0.213 ± 0.017	1.572TyrAsp: 1.572 ± 0.048	1.042TyrGlu: 1.042 ± 0.035	0.773TyrPhe: 0.773 ± 0.033	2.113TyrGly: 2.113 ± 0.056	0.447TyrHis: 0.447 ± 0.022	0.81TyrIle: 0.81 ± 0.035	0.509TyrLys: 0.509 ± 0.026	1.967TyrLeu: 1.967 ± 0.05	0.393TyrMet: 0.393 ± 0.025	0.559TyrAsn: 0.559 ± 0.033	1.018TyrPro: 1.018 ± 0.033	0.628TyrGln: 0.628 ± 0.033	1.878TyrArg: 1.878 ± 0.051	1.121TyrSer: 1.121 ± 0.039	1.003TyrThr: 1.003 ± 0.035	1.603TyrVal: 1.603 ± 0.05	0.32TyrTrp: 0.32 ± 0.021	0.538TyrTyr: 0.538 ± 0.033	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.005XaaXaa: 0.005 ± 0.005

Statistics based on 3084 proteins (807437 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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